Restriction map for
pF1KB3088
(Linear) (Six-base) MAPSORT of: KIBB3088.seq Check: 7135 from: 1 to: 1254
KIBB3088.orf
With 182 enzymes: SgfI *
February 28, 2007 11:23 ..
AccI GT'mk_AC
Cuts at: 0 887 1254
Size: 887 367
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 738 1254
Size: 738 516
AlwNI CAG_nnn'CTG
Cuts at: 0 170 1090 1254
Size: 170 920 164
Fragments arranged by size:
920 170 164
AvaI C'yCGr_G
Cuts at: 0 1079 1254
Size: 1079 175
BamHI G'GATC_C
Cuts at: 0 474 1254
Size: 474 780
BanI G'GyrC_C
Cuts at: 0 65 139 184 202 279 877 1254
Size: 65 74 45 18 77 598 377
Fragments arranged by size:
598 377 77 74 65 45 18
BanII G_rGCy'C
Cuts at: 0 239 1254
Size: 239 1015
BbeI G_GCGC'C
Cuts at: 0 188 283 1254
Size: 188 95 971
Fragments arranged by size:
971 188 95
BbvCI CC'TCA_GC
Cuts at: 0 61 1254
Size: 61 1193
BclI T'GATC_A
Cuts at: 0 417 546 1254
Size: 417 129 708
Fragments arranged by size:
708 417 129
BglI GCCn_nnn'nGGC
Cuts at: 0 161 1254
Size: 161 1093
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 106 293 974 1254
Size: 106 187 681 280
Fragments arranged by size:
681 280 187 106
Bpu10I CC'TnA_GC
Cuts at: 0 61 914 1254
Size: 61 853 340
Fragments arranged by size:
853 340 61
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 476 1254
Size: 476 778
BsaHI Gr'CG_yC
Cuts at: 0 185 280 1254
Size: 185 95 974
Fragments arranged by size:
974 185 95
BsaWI w'CCGG_w
Cuts at: 0 477 1254
Size: 477 777
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 472 502 1254
Size: 472 30 752
Fragments arranged by size:
752 472 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 247 280 283 286 1254
Size: 247 33 3 3 968
Fragments arranged by size:
968 247 33 3 3
BseYI C'CCAG_C
Cuts at: 0 208 1254
Size: 208 1046
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 368 1031 1254
Size: 368 663 223
Fragments arranged by size:
663 368 223
BsiHKAI G_wGCw'C
Cuts at: 0 729 1254
Size: 729 525
Bsp1286I G_dGCh'C
Cuts at: 0 239 729 1254
Size: 239 490 525
Fragments arranged by size:
525 490 239
BspEI T'CCGG_A
Cuts at: 0 477 1254
Size: 477 777
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 89 1254
Size: 89 1165
BsrDI GCAATG_nn'
Cuts at: 0 908 1130 1254
Size: 908 222 124
Fragments arranged by size:
908 222 124
BsrGI T'GTAC_A
Cuts at: 0 680 1254
Size: 680 574
BssHII G'CGCG_C
Cuts at: 0 145 1254
Size: 145 1109
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1090 1254
Size: 1090 164
BstEII G'GTnAC_C
Cuts at: 0 747 1254
Size: 747 507
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1102 1254
Size: 1102 152
BstYI r'GATC_y
Cuts at: 0 11 474 1254
Size: 11 463 780
Fragments arranged by size:
780 463 11
BstZ17I GTA'TAC
Cuts at: 0 888 1254
Size: 888 366
BtgI C'CryG_G
Cuts at: 0 74 284 1254
Size: 74 210 970
Fragments arranged by size:
970 210 74
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1170 1254
Size: 1170 84
BtsI GCAGTG_nn'
Cuts at: 0 707 817 1247 1254
Size: 707 110 430 7
Fragments arranged by size:
707 430 110 7
EaeI y'GGCC_r
Cuts at: 0 153 1254
Size: 153 1101
EarI CTCTTCn'nnn_
Cuts at: 0 995 1254
Size: 995 259
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 106 293 738 974 1254
Size: 106 187 445 236 280
Fragments arranged by size:
445 280 236 187 106
EcoNI CCTnn'n_nnAGG
Cuts at: 0 118 1254
Size: 118 1136
EcoO109I rG'GnC_Cy
Cuts at: 0 167 177 1156 1254
Size: 167 10 979 98
Fragments arranged by size:
979 167 98 10
HaeII r_GCGC'y
Cuts at: 0 188 283 1254
Size: 188 95 971
Fragments arranged by size:
971 188 95
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 373 387 405 419 472 504 890 922
Size: 373 14 18 14 53 32 386 32
Cuts at: 922 1254
Size: 332
Fragments arranged by size:
386 373 332 53 32 32 18 14
14
HincII GTy'rAC
Cuts at: 0 222 1254
Size: 222 1032
HindIII A'AGCT_T
Cuts at: 0 353 804 1254
Size: 353 451 450
Fragments arranged by size:
451 450 353
KasI G'GCGC_C
Cuts at: 0 184 279 1254
Size: 184 95 975
Fragments arranged by size:
975 184 95
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 272 603 1254
Size: 272 331 651
Fragments arranged by size:
651 331 272
MspA1I CmG'CkG
Cuts at: 0 76 190 286 1254
Size: 76 114 96 968
Fragments arranged by size:
968 114 96 76
NarI GG'CG_CC
Cuts at: 0 185 280 1254
Size: 185 95 974
Fragments arranged by size:
974 185 95
NspI r_CATG'y
Cuts at: 0 541 1254
Size: 541 713
PfoI T'CCnGG_A
Cuts at: 0 391 1254
Size: 391 863
PpuMI rG'GwC_Cy
Cuts at: 0 167 177 1156 1254
Size: 167 10 979 98
Fragments arranged by size:
979 167 98 10
PsiI TTA'TAA
Cuts at: 0 359 1254
Size: 359 895
PspXI vC'TCGA_Gb
Cuts at: 0 1079 1254
Size: 1079 175
PstI C_TGCA'G
Cuts at: 0 41 101 119 1254
Size: 41 60 18 1135
Fragments arranged by size:
1135 60 41 18
SacII CC_GC'GG
Cuts at: 0 77 287 1254
Size: 77 210 967
Fragments arranged by size:
967 210 77
ScaI AGT'ACT
Cuts at: 0 1045 1254
Size: 1045 209
SfcI C'TryA_G
Cuts at: 0 37 97 115 1254
Size: 37 60 18 1139
Fragments arranged by size:
1139 60 37 18
SfoI GGC'GCC
Cuts at: 0 186 281 1254
Size: 186 95 973
Fragments arranged by size:
973 186 95
SmlI C'TyrA_G
Cuts at: 0 491 1079 1254
Size: 491 588 175
Fragments arranged by size:
588 491 175
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 705 1254
Size: 705 549
TatI w'GTAC_w
Cuts at: 0 680 1043 1254
Size: 680 363 211
Fragments arranged by size:
680 363 211
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 809 841 1254
Size: 809 32 413
Fragments arranged by size:
809 413 32
XhoI C'TCGA_G
Cuts at: 0 1079 1254
Size: 1079 175
XmnI GAAnn'nnTTC
Cuts at: 0 1148 1254
Size: 1148 106
Enzymes that do cut:
AccI AcuI AlwNI AvaI BamHI BanI BanII BbeI
BbvCI BclI BglI BpmI Bpu10I BpuEI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspEI BspMI
BsrDI BsrGI BssHII BstAPI BstEII BstXI BstYI BstZ17I
BtgI BtgZI BtsI EaeI EarI Eco57MI EcoNI EcoO109I
HaeII Hin4I HincII HindIII KasI MmeI MspA1I NarI
NspI PfoI PpuMI PsiI PspXI PstI SacII ScaI
SfcI SfoI SmlI TaqII TatI TstI XhoI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BbsI BcgI BciVI BfrBI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BsaI
BsaAI BsaBI BsiEI BsiWI BsmI BsmBI BspHI BsrBI
BsrFI BssSI BstBI Bsu36I ClaI CspCI DraI DraIII
DrdI EagI EciI EcoICRI EcoRI EcoRV FalI FseI
FspI FspAI HpaI KpnI MfeI MluI MscI MslI
NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI
PacI PasI PciI PflMI PmeI PmlI PpiI PshAI
PspOMI PsrI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI SexAI SfiI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI StuI StyI SwaI Tth111I
XbaI XcmI XmaI ZraI