Restriction map for
pF1KB3089
(Linear) (Six-base) MAPSORT of: KIBB3089.seq Check: 3647 from: 1 to: 1425
KIBB3089.orf
With 182 enzymes: SgfI *
February 28, 2007 11:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1383 1425
Size: 1383 42
AatII G_ACGT'C
Cuts at: 0 623 1425
Size: 623 802
AccI GT'mk_AC
Cuts at: 0 617 1425
Size: 617 808
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 158 1009 1163 1425
Size: 158 851 154 262
Fragments arranged by size:
851 262 158 154
AfeI AGC'GCT
Cuts at: 0 808 1425
Size: 808 617
AhdI GACnn_n'nnGTC
Cuts at: 0 339 1425
Size: 339 1086
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 31 1425
Size: 31 1394
AlwNI CAG_nnn'CTG
Cuts at: 0 51 1425
Size: 51 1374
ApoI r'AATT_y
Cuts at: 0 118 1425
Size: 118 1307
AvaI C'yCGr_G
Cuts at: 0 582 1425
Size: 582 843
BanI G'GyrC_C
Cuts at: 0 273 1102 1371 1425
Size: 273 829 269 54
Fragments arranged by size:
829 273 269 54
BanII G_rGCy'C
Cuts at: 0 351 486 902 1425
Size: 351 135 416 523
Fragments arranged by size:
523 416 351 135
BbsI GAAGACnn'nnnn_
Cuts at: 0 146 997 1011 1425
Size: 146 851 14 414
Fragments arranged by size:
851 414 146 14
BbvCI CC'TCA_GC
Cuts at: 0 149 1425
Size: 149 1276
BciVI GTATCCnnnnn_n'
Cuts at: 0 463 1425
Size: 463 962
BmrI ACTGGGnnnn_n'
Cuts at: 0 358 1425
Size: 358 1067
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1222 1425
Size: 1222 203
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 28 1425
Size: 28 1397
Bpu10I CC'TnA_GC
Cuts at: 0 149 350 1425
Size: 149 201 1075
Fragments arranged by size:
1075 201 149
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 927 1425
Size: 927 498
BsaHI Gr'CG_yC
Cuts at: 0 620 1425
Size: 620 805
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 418 448 793 823 1333 1363 1425
Size: 418 30 345 30 510 30 62
Fragments arranged by size:
510 418 345 62 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 442 445 1425
Size: 442 3 980
Fragments arranged by size:
980 442 3
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 891 1425
Size: 891 534
BsiHKAI G_wGCw'C
Cuts at: 0 799 1425
Size: 799 626
Bsp1286I G_dGCh'C
Cuts at: 0 351 486 799 902 1425
Size: 351 135 313 103 523
Fragments arranged by size:
523 351 313 135 103
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1383 1425
Size: 1383 42
BsrDI GCAATG_nn'
Cuts at: 0 497 1425
Size: 497 928
BsrGI T'GTAC_A
Cuts at: 0 296 1425
Size: 296 1129
BssSI C'ACGA_G
Cuts at: 0 19 802 1425
Size: 19 783 623
Fragments arranged by size:
783 623 19
BstXI CCAn_nnnn'nTGG
Cuts at: 0 390 493 1425
Size: 390 103 932
Fragments arranged by size:
932 390 103
Bsu36I CC'TnA_GG
Cuts at: 0 127 1425
Size: 127 1298
BtgI C'CryG_G
Cuts at: 0 63 188 237 371 1106 1425
Size: 63 125 49 134 735 319
Fragments arranged by size:
735 319 134 125 63 49
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 266 301 1425
Size: 266 35 1124
Fragments arranged by size:
1124 266 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 319 1425
Size: 319 1106
EaeI y'GGCC_r
Cuts at: 0 32 74 287 1109 1425
Size: 32 42 213 822 316
Fragments arranged by size:
822 316 213 42 32
EarI CTCTTCn'nnn_
Cuts at: 0 87 826 1222 1425
Size: 87 739 396 203
Fragments arranged by size:
739 396 203 87
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 28 158 1009 1163 1425
Size: 28 130 851 154 262
Fragments arranged by size:
851 262 154 130 28
EcoO109I rG'GnC_Cy
Cuts at: 0 131 644 1425
Size: 131 513 781
Fragments arranged by size:
781 513 131
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 903 1344 1425
Size: 903 441 81
Fragments arranged by size:
903 441 81
HaeII r_GCGC'y
Cuts at: 0 810 1425
Size: 810 615
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 86 118 325 357 728 760 984 1016
Size: 86 32 207 32 371 32 224 32
Cuts at: 1016 1333 1365 1425
Size: 317 32 60
Fragments arranged by size:
371 317 224 207 86 60 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 528 690 1425
Size: 528 162 735
Fragments arranged by size:
735 528 162
MslI CAynn'nnrTG
Cuts at: 0 328 1425
Size: 328 1097
MspA1I CmG'CkG
Cuts at: 0 65 148 373 553 926 1425
Size: 65 83 225 180 373 499
Fragments arranged by size:
499 373 225 180 83 65
NcoI C'CATG_G
Cuts at: 0 237 1425
Size: 237 1188
NspI r_CATG'y
Cuts at: 0 688 1425
Size: 688 737
PflMI CCAn_nnn'nTGG
Cuts at: 0 465 1425
Size: 465 960
PfoI T'CCnGG_A
Cuts at: 0 171 1425
Size: 171 1254
PpuMI rG'GwC_Cy
Cuts at: 0 131 644 1425
Size: 131 513 781
Fragments arranged by size:
781 513 131
PvuII CAG'CTG
Cuts at: 0 148 553 1425
Size: 148 405 872
Fragments arranged by size:
872 405 148
SacII CC_GC'GG
Cuts at: 0 66 374 1425
Size: 66 308 1051
Fragments arranged by size:
1051 308 66
SanDI GG'GwC_CC
Cuts at: 0 644 1425
Size: 644 781
SfcI C'TryA_G
Cuts at: 0 864 1425
Size: 864 561
SmlI C'TyrA_G
Cuts at: 0 906 1425
Size: 906 519
StuI AGG'CCT
Cuts at: 0 862 1081 1425
Size: 862 219 344
Fragments arranged by size:
862 344 219
StyI C'CwwG_G
Cuts at: 0 237 477 878 1425
Size: 237 240 401 547
Fragments arranged by size:
547 401 240 237
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 261 1425
Size: 261 1164
TatI w'GTAC_w
Cuts at: 0 296 1425
Size: 296 1129
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 386 418 793 825 1044 1076 1425
Size: 386 32 375 32 219 32 349
Fragments arranged by size:
386 375 349 219 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 454 1425
Size: 454 971
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 324 676 1425
Size: 324 352 749
Fragments arranged by size:
749 352 324
ZraI GAC'GTC
Cuts at: 0 621 1425
Size: 621 804
Enzymes that do cut:
AarI AatII AccI AcuI AfeI AhdI AlfI AlwNI
ApoI AvaI BanI BanII BbsI BbvCI BciVI BmrI
BplI BpmI Bpu10I BpuEI BsaHI BsaXI BseRI BsgI
BsiHKAI Bsp1286I BspMI BsrDI BsrGI BssSI BstXI Bsu36I
BtgI CspCI DrdI EaeI EarI Eco57MI EcoO109I FalI
HaeII Hin4I HincII MslI MspA1I NcoI NspI PflMI
PfoI PpuMI PvuII SacII SanDI SfcI SmlI StuI
StyI TaqII TatI TstI Tth111I XcmI ZraI
Enzymes that do not cut:
Acc65I AclI AflII AflIII AgeI AleI AloI ApaI
ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbeI
BcgI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmtI BsaI BsaAI BsaBI BsaWI BseYI BsiEI BsiWI
BsmI BsmBI BspEI BspHI BsrBI BsrFI BssHII BstAPI
BstBI BstEII BstYI BstZ17I BtgZI BtsI ClaI DraI
DraIII EagI EciI EcoICRI EcoNI EcoRI EcoRV FseI
FspI FspAI HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI NaeI NarI NdeI NgoMIV NheI NotI
NruI NsiI PacI PasI PciI PmeI PmlI PpiI
PshAI PsiI PspOMI PspXI PsrI PstI PvuI RsrII
SacI SalI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
SwaI XbaI XhoI XmaI XmnI