Restriction map for
pF1KB3091
(Linear) (Six-base) MAPSORT of: KIBB3091.seq Check: 4446 from: 1 to: 2802
KIBB3091.orf
With 182 enzymes: SgfI *
January 9, 2009 11:10 ..
AccI GT'mk_AC
Cuts at: 0 1687 1964 2802
Size: 1687 277 838
Fragments arranged by size:
1687 838 277
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 870 1739 2441 2759 2802
Size: 870 869 702 318 43
Fragments arranged by size:
870 869 702 318 43
AflIII A'CryG_T
Cuts at: 0 1829 2400 2802
Size: 1829 571 402
Fragments arranged by size:
1829 571 402
AgeI A'CCGG_T
Cuts at: 0 725 2802
Size: 725 2077
AleI CACnn'nnGTG
Cuts at: 0 97 2802
Size: 97 2705
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2631 2802
Size: 2631 171
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1832 1864 2802
Size: 1832 32 938
Fragments arranged by size:
1832 938 32
AlwNI CAG_nnn'CTG
Cuts at: 0 975 1260 2802
Size: 975 285 1542
Fragments arranged by size:
1542 975 285
ApaLI G'TGCA_C
Cuts at: 0 1547 2802
Size: 1547 1255
ApoI r'AATT_y
Cuts at: 0 249 529 662 1080 1399 1648 2802
Size: 249 280 133 418 319 249 1154
Fragments arranged by size:
1154 418 319 280 249 249 133
AseI AT'TA_AT
Cuts at: 0 1486 2230 2802
Size: 1486 744 572
Fragments arranged by size:
1486 744 572
AvaI C'yCGr_G
Cuts at: 0 161 2129 2802
Size: 161 1968 673
Fragments arranged by size:
1968 673 161
BanII G_rGCy'C
Cuts at: 0 927 2802
Size: 927 1875
BbsI GAAGACnn'nnnn_
Cuts at: 0 401 2802
Size: 401 2401
BclI T'GATC_A
Cuts at: 0 188 543 668 2466 2802
Size: 188 355 125 1798 336
Fragments arranged by size:
1798 355 336 188 125
BglII A'GATC_T
Cuts at: 0 270 1155 1503 2802
Size: 270 885 348 1299
Fragments arranged by size:
1299 885 348 270
BlpI GC'TnA_GC
Cuts at: 0 1799 2802
Size: 1799 1003
Bme1580I G_kGCm'C
Cuts at: 0 1551 2802
Size: 1551 1251
BmrI ACTGGGnnnn_n'
Cuts at: 0 2054 2802
Size: 2054 748
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 641 2802
Size: 641 2161
Bpu10I CC'TnA_GC
Cuts at: 0 2510 2802
Size: 2510 292
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 580 992 2572 2802
Size: 580 412 1580 230
Fragments arranged by size:
1580 580 412 230
BsaI GGTCTCn'nnnn_
Cuts at: 0 2419 2802
Size: 2419 383
BsaAI yAC'GTr
Cuts at: 0 1830 2802
Size: 1830 972
BsaBI GATnn'nnATC
Cuts at: 0 542 1379 1427 2802
Size: 542 837 48 1375
Fragments arranged by size:
1375 837 542 48
BsaWI w'CCGG_w
Cuts at: 0 725 2802
Size: 725 2077
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1832 1862 2548 2578 2647 2677 2802
Size: 1832 30 686 30 69 30 125
Fragments arranged by size:
1832 686 125 69 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 559 623 671 1084 1304 2569 2802
Size: 559 64 48 413 220 1265 233
Fragments arranged by size:
1265 559 413 233 220 64 48
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 28 1514 2802
Size: 28 1486 1288
Fragments arranged by size:
1486 1288 28
BsiEI CG_ry'CG
Cuts at: 0 44 114 2802
Size: 44 70 2688
Fragments arranged by size:
2688 70 44
BsiHKAI G_wGCw'C
Cuts at: 0 1551 1845 2802
Size: 1551 294 957
Fragments arranged by size:
1551 957 294
BsmBI CGTCTCn'nnnn_
Cuts at: 0 13 2802
Size: 13 2789
Bsp1286I G_dGCh'C
Cuts at: 0 927 1551 1845 2802
Size: 927 624 294 957
Fragments arranged by size:
957 927 624 294
BspHI T'CATG_A
Cuts at: 0 2352 2802
Size: 2352 450
BsrDI GCAATG_nn'
Cuts at: 0 2306 2802
Size: 2306 496
BsrFI r'CCGG_y
Cuts at: 0 204 725 2802
Size: 204 521 2077
Fragments arranged by size:
2077 521 204
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2497 2802
Size: 2497 305
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1639 2175 2802
Size: 1639 536 627
Fragments arranged by size:
1639 627 536
BstYI r'GATC_y
Cuts at: 0 270 1155 1503 2802
Size: 270 885 348 1299
Fragments arranged by size:
1299 885 348 270
BstZ17I GTA'TAC
Cuts at: 0 1688 1965 2802
Size: 1688 277 837
Fragments arranged by size:
1688 837 277
Bsu36I CC'TnA_GG
Cuts at: 0 2206 2802
Size: 2206 596
DraI TTT'AAA
Cuts at: 0 1676 2802
Size: 1676 1126
EaeI y'GGCC_r
Cuts at: 0 41 155 468 2369 2802
Size: 41 114 313 1901 433
Fragments arranged by size:
1901 433 313 114 41
EagI C'GGCC_G
Cuts at: 0 41 2802
Size: 41 2761
EarI CTCTTCn'nnn_
Cuts at: 0 1676 2247 2417 2802
Size: 1676 571 170 385
Fragments arranged by size:
1676 571 385 170
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 641 870 1739 2441 2759 2802
Size: 641 229 869 702 318 43
Fragments arranged by size:
869 702 641 318 229 43
EcoNI CCTnn'n_nnAGG
Cuts at: 0 937 2665 2802
Size: 937 1728 137
Fragments arranged by size:
1728 937 137
EcoO109I rG'GnC_Cy
Cuts at: 0 717 2802
Size: 717 2085
EcoRI G'AATT_C
Cuts at: 0 529 662 1399 2802
Size: 529 133 737 1403
Fragments arranged by size:
1403 737 529 133
EcoRV GAT'ATC
Cuts at: 0 1629 2592 2802
Size: 1629 963 210
Fragments arranged by size:
1629 963 210
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 292 1156 2276 2802
Size: 292 864 1120 526
Fragments arranged by size:
1120 864 526 292
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 535 567 2576 2608 2647 2679 2802
Size: 535 32 2009 32 39 32 123
Fragments arranged by size:
2009 535 123 39 32 32 32
HincII GTy'rAC
Cuts at: 0 1026 1481 2802
Size: 1026 455 1321
Fragments arranged by size:
1321 1026 455
HindIII A'AGCT_T
Cuts at: 0 1091 2802
Size: 1091 1711
HpaI GTT'AAC
Cuts at: 0 1026 2802
Size: 1026 1776
MfeI C'AATT_G
Cuts at: 0 2152 2802
Size: 2152 650
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 11 462 875 2168 2802
Size: 11 451 413 1293 634
Fragments arranged by size:
1293 634 451 413 11
MscI TGG'CCA
Cuts at: 0 470 2371 2802
Size: 470 1901 431
Fragments arranged by size:
1901 470 431
MslI CAynn'nnrTG
Cuts at: 0 97 1919 2173 2351 2802
Size: 97 1822 254 178 451
Fragments arranged by size:
1822 451 254 178 97
NaeI GCC'GGC
Cuts at: 0 206 2802
Size: 206 2596
NdeI CA'TA_TG
Cuts at: 0 1854 2802
Size: 1854 948
NgoMIV G'CCGG_C
Cuts at: 0 204 2802
Size: 204 2598
NspI r_CATG'y
Cuts at: 0 78 1918 2337 2404 2518 2802
Size: 78 1840 419 67 114 284
Fragments arranged by size:
1840 419 284 114 78 67
PasI CC'CwG_GG
Cuts at: 0 180 2802
Size: 180 2622
PciI A'CATG_T
Cuts at: 0 2400 2802
Size: 2400 402
PmlI CAC'GTG
Cuts at: 0 1830 2802
Size: 1830 972
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1832 1864 2802
Size: 1832 32 938
Fragments arranged by size:
1832 938 32
PpuMI rG'GwC_Cy
Cuts at: 0 717 2802
Size: 717 2085
PsiI TTA'TAA
Cuts at: 0 308 2802
Size: 308 2494
PstI C_TGCA'G
Cuts at: 0 29 227 2802
Size: 29 198 2575
Fragments arranged by size:
2575 198 29
ScaI AGT'ACT
Cuts at: 0 1620 2057 2802
Size: 1620 437 745
Fragments arranged by size:
1620 745 437
SfcI C'TryA_G
Cuts at: 0 25 223 1622 2802
Size: 25 198 1399 1180
Fragments arranged by size:
1399 1180 198 25
SmaI CCC'GGG
Cuts at: 0 163 2802
Size: 163 2639
SmlI C'TyrA_G
Cuts at: 0 559 1007 2551 2802
Size: 559 448 1544 251
Fragments arranged by size:
1544 559 448 251
SphI G_CATG'C
Cuts at: 0 78 1918 2802
Size: 78 1840 884
Fragments arranged by size:
1840 884 78
SspI AAT'ATT
Cuts at: 0 1749 2802
Size: 1749 1053
StyI C'CwwG_G
Cuts at: 0 335 2366 2666 2802
Size: 335 2031 300 136
Fragments arranged by size:
2031 335 300 136
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 433 2063 2802
Size: 433 1630 739
Fragments arranged by size:
1630 739 433
TatI w'GTAC_w
Cuts at: 0 194 1618 2055 2802
Size: 194 1424 437 747
Fragments arranged by size:
1424 747 437 194
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 438 478 1999 2802
Size: 438 40 1521 803
Fragments arranged by size:
1521 803 438 40
XmaI C'CCGG_G
Cuts at: 0 161 2802
Size: 161 2641
XmnI GAAnn'nnTTC
Cuts at: 0 398 2802
Size: 398 2404
Enzymes that do cut:
AccI AcuI AflIII AgeI AleI AlfI AloI AlwNI
ApaLI ApoI AseI AvaI BanII BbsI BclI BglII
BlpI Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaWI BsaXI BseRI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspHI BsrDI BsrFI BstAPI BstXI BstYI BstZ17I
Bsu36I DraI EaeI EagI EarI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI Hin4I HincII HindIII HpaI MfeI
MmeI MscI MslI NaeI NdeI NgoMIV NspI PasI
PciI PmlI PpiI PpuMI PsiI PstI ScaI SfcI
SmaI SmlI SphI SspI StyI TaqII TatI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AhdI ApaI
AscI AsiSI AvrII BaeI BamHI BanI BbeI BbvCI
BcgI BciVI BfrBI BglI BmgBI BmtI BplI BsaHI
BseYI BsiWI BsmI BspEI BspMI BsrBI BsrGI BssHII
BssSI BstBI BstEII BtgI BtgZI BtsI ClaI CspCI
DraIII DrdI EciI EcoICRI FseI FspI FspAI HaeII
KasI KpnI MluI MspA1I NarI NcoI NheI NotI
NruI NsiI PacI PflMI PfoI PmeI PshAI PspOMI
PspXI PsrI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI SexAI SfiI SfoI SgfI SgrAI
SnaBI SpeI SrfI StuI SwaI TstI Tth111I XbaI
XhoI ZraI