(Linear) (Six-base) MAPSORT of: KIBB3092.seq Check: 8100 from: 1 to: 2148 KIBB3092.orf With 182 enzymes: SgfI * July 18, 2007 13:36 .. AatII G_ACGT'C Cuts at: 0 480 2148 Size: 480 1668 AccI GT'mk_AC Cuts at: 0 487 2148 Size: 487 1661 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 400 632 2148 Size: 400 232 1516 Fragments arranged by size: 1516 400 232 AflII C'TTAA_G Cuts at: 0 180 2148 Size: 180 1968 AflIII A'CryG_T Cuts at: 0 846 1716 2148 Size: 846 870 432 Fragments arranged by size: 870 846 432 AhdI GACnn_n'nnGTC Cuts at: 0 476 2148 Size: 476 1672 AlwNI CAG_nnn'CTG Cuts at: 0 976 2148 Size: 976 1172 ApoI r'AATT_y Cuts at: 0 895 916 950 1048 1370 1556 1900 2148 Size: 895 21 34 98 322 186 344 248 Fragments arranged by size: 895 344 322 248 186 98 34 21 AvaI C'yCGr_G Cuts at: 0 443 995 1433 2148 Size: 443 552 438 715 Fragments arranged by size: 715 552 443 438 BamHI G'GATC_C Cuts at: 0 1042 2148 Size: 1042 1106 BanII G_rGCy'C Cuts at: 0 393 1764 2148 Size: 393 1371 384 Fragments arranged by size: 1371 393 384 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 3 37 2148 Size: 3 34 2111 Fragments arranged by size: 2111 34 3 BclI T'GATC_A Cuts at: 0 222 1404 2148 Size: 222 1182 744 Fragments arranged by size: 1182 744 222 BfrBI ATG'CAT Cuts at: 0 628 1185 2148 Size: 628 557 963 Fragments arranged by size: 963 628 557 BglI GCCn_nnn'nGGC Cuts at: 0 1701 2148 Size: 1701 447 BglII A'GATC_T Cuts at: 0 411 2148 Size: 411 1737 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 466 1558 2148 Size: 466 1092 590 Fragments arranged by size: 1092 590 466 Bpu10I CC'TnA_GC Cuts at: 0 143 2148 Size: 143 2005 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 650 2143 2148 Size: 650 1493 5 Fragments arranged by size: 1493 650 5 BsaHI Gr'CG_yC Cuts at: 0 477 2148 Size: 477 1671 BsaWI w'CCGG_w Cuts at: 0 782 1453 2148 Size: 782 671 695 Fragments arranged by size: 782 695 671 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 95 125 464 494 1525 1555 1787 1817 Size: 95 30 339 30 1031 30 232 30 Cuts at: 1817 2148 Size: 331 Fragments arranged by size: 1031 339 331 232 95 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1825 2148 Size: 1825 323 BseYI C'CCAG_C Cuts at: 0 157 1763 2148 Size: 157 1606 385 Fragments arranged by size: 1606 385 157 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 287 2148 Size: 287 1861 BsiHKAI G_wGCw'C Cuts at: 0 19 393 2148 Size: 19 374 1755 Fragments arranged by size: 1755 374 19 BsmI GAATG_Cn' Cuts at: 0 1122 2148 Size: 1122 1026 BsmBI CGTCTCn'nnnn_ Cuts at: 0 484 2148 Size: 484 1664 Bsp1286I G_dGCh'C Cuts at: 0 19 393 1764 2148 Size: 19 374 1371 384 Fragments arranged by size: 1371 384 374 19 BspEI T'CCGG_A Cuts at: 0 782 1453 2148 Size: 782 671 695 Fragments arranged by size: 782 695 671 BspHI T'CATG_A Cuts at: 0 590 1623 2148 Size: 590 1033 525 Fragments arranged by size: 1033 590 525 BsrDI GCAATG_nn' Cuts at: 0 1673 2148 Size: 1673 475 BstEII G'GTnAC_C Cuts at: 0 1521 1893 2148 Size: 1521 372 255 Fragments arranged by size: 1521 372 255 BstYI r'GATC_y Cuts at: 0 411 813 1042 2148 Size: 411 402 229 1106 Fragments arranged by size: 1106 411 402 229 Bsu36I CC'TnA_GG Cuts at: 0 697 2148 Size: 697 1451 BtgI C'CryG_G Cuts at: 0 1696 2148 Size: 1696 452 BtsI GCAGTG_nn' Cuts at: 0 168 2148 Size: 168 1980 DraI TTT'AAA Cuts at: 0 453 633 1239 2148 Size: 453 180 606 909 Fragments arranged by size: 909 606 453 180 EaeI y'GGCC_r Cuts at: 0 424 2148 Size: 424 1724 EarI CTCTTCn'nnn_ Cuts at: 0 398 724 1159 1794 2148 Size: 398 326 435 635 354 Fragments arranged by size: 635 435 398 354 326 EcoICRI GAG'CTC Cuts at: 0 391 2148 Size: 391 1757 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 400 466 632 1558 2148 Size: 400 66 166 926 590 Fragments arranged by size: 926 590 400 166 66 EcoO109I rG'GnC_Cy Cuts at: 0 186 1567 2148 Size: 186 1381 581 Fragments arranged by size: 1381 581 186 EcoRI G'AATT_C Cuts at: 0 895 950 2148 Size: 895 55 1198 Fragments arranged by size: 1198 895 55 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 970 2148 Size: 970 1178 HaeII r_GCGC'y Cuts at: 0 526 2148 Size: 526 1622 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 462 494 547 579 1194 1226 2148 Size: 462 32 53 32 615 32 922 Fragments arranged by size: 922 615 462 53 32 32 32 HindIII A'AGCT_T Cuts at: 0 1918 2072 2148 Size: 1918 154 76 Fragments arranged by size: 1918 154 76 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 942 1445 2117 2148 Size: 942 503 672 31 Fragments arranged by size: 942 672 503 31 MscI TGG'CCA Cuts at: 0 426 2148 Size: 426 1722 MslI CAynn'nnrTG Cuts at: 0 131 623 2148 Size: 131 492 1525 Fragments arranged by size: 1525 492 131 MspA1I CmG'CkG Cuts at: 0 228 1501 1767 2148 Size: 228 1273 266 381 Fragments arranged by size: 1273 381 266 228 NdeI CA'TA_TG Cuts at: 0 2013 2148 Size: 2013 135 NsiI A_TGCA'T Cuts at: 0 630 1187 2148 Size: 630 557 961 Fragments arranged by size: 961 630 557 NspI r_CATG'y Cuts at: 0 850 1329 1720 2148 Size: 850 479 391 428 Fragments arranged by size: 850 479 428 391 PasI CC'CwG_GG Cuts at: 0 970 2148 Size: 970 1178 PciI A'CATG_T Cuts at: 0 846 1716 2148 Size: 846 870 432 Fragments arranged by size: 870 846 432 PflMI CCAn_nnn'nTGG Cuts at: 0 976 1711 2148 Size: 976 735 437 Fragments arranged by size: 976 735 437 PfoI T'CCnGG_A Cuts at: 0 246 2148 Size: 246 1902 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 122 154 2148 Size: 122 32 1994 Fragments arranged by size: 1994 122 32 PpuMI rG'GwC_Cy Cuts at: 0 186 2148 Size: 186 1962 PshAI GACnn'nnGTC Cuts at: 0 1719 2148 Size: 1719 429 PspXI vC'TCGA_Gb Cuts at: 0 1433 2148 Size: 1433 715 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 342 374 2148 Size: 342 32 1774 Fragments arranged by size: 1774 342 32 PstI C_TGCA'G Cuts at: 0 810 2148 Size: 810 1338 PvuII CAG'CTG Cuts at: 0 228 1501 1767 2148 Size: 228 1273 266 381 Fragments arranged by size: 1273 381 266 228 SacI G_AGCT'C Cuts at: 0 393 2148 Size: 393 1755 SapI GCTCTTCn'nnn_ Cuts at: 0 398 2148 Size: 398 1750 ScaI AGT'ACT Cuts at: 0 421 2148 Size: 421 1727 SfcI C'TryA_G Cuts at: 0 806 1935 2148 Size: 806 1129 213 Fragments arranged by size: 1129 806 213 SmlI C'TyrA_G Cuts at: 0 180 665 1433 2122 2148 Size: 180 485 768 689 26 Fragments arranged by size: 768 689 485 180 26 StyI C'CwwG_G Cuts at: 0 189 1342 2148 Size: 189 1153 806 Fragments arranged by size: 1153 806 189 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1057 2148 Size: 1057 1091 TatI w'GTAC_w Cuts at: 0 349 419 2148 Size: 349 70 1729 Fragments arranged by size: 1729 349 70 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 464 496 1787 1819 2148 Size: 464 32 1291 32 329 Fragments arranged by size: 1291 464 329 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1533 2148 Size: 1533 615 XhoI C'TCGA_G Cuts at: 0 1433 2148 Size: 1433 715 XmnI GAAnn'nnTTC Cuts at: 0 653 2148 Size: 653 1495 ZraI GAC'GTC Cuts at: 0 478 2148 Size: 478 1670 Enzymes that do cut: AatII AccI AcuI AflII AflIII AhdI AlwNI ApoI AvaI BamHI BanII BcgI BclI BfrBI BglI BglII BpmI Bpu10I BpuEI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BsrDI BstEII BstYI Bsu36I BtgI BtsI DraI EaeI EarI EcoICRI Eco57MI EcoO109I EcoRI FalI HaeII Hin4I HindIII MmeI MscI MslI MspA1I NdeI NsiI NspI PasI PciI PflMI PfoI PpiI PpuMI PshAI PspXI PsrI PstI PvuII SacI SapI ScaI SfcI SmlI StyI TaqII TatI TstI XcmI XhoI XmnI ZraI Enzymes that do not cut: AarI Acc65I AclI AfeI AgeI AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI AvrII BaeI BanI BbeI BbsI BbvCI BciVI BlpI Bme1580I BmgBI BmrI BmtI BplI BsaI BsaAI BsaBI BsiEI BsiWI BspMI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstXI BstZ17I BtgZI ClaI CspCI DraIII DrdI EagI EciI EcoNI EcoRV FseI FspI FspAI HincII HpaI KasI KpnI MfeI MluI NaeI NarI NcoI NgoMIV NheI NotI NruI PacI PmeI PmlI PsiI PspOMI PvuI RsrII SacII SalI SanDI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI Tth111I XbaI XmaI