Restriction map for
pF1KB3092
(Linear) (Six-base) MAPSORT of: KIBB3092.seq Check: 8100 from: 1 to: 2148
KIBB3092.orf
With 182 enzymes: SgfI *
July 18, 2007 13:36 ..
AatII G_ACGT'C
Cuts at: 0 480 2148
Size: 480 1668
AccI GT'mk_AC
Cuts at: 0 487 2148
Size: 487 1661
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 400 632 2148
Size: 400 232 1516
Fragments arranged by size:
1516 400 232
AflII C'TTAA_G
Cuts at: 0 180 2148
Size: 180 1968
AflIII A'CryG_T
Cuts at: 0 846 1716 2148
Size: 846 870 432
Fragments arranged by size:
870 846 432
AhdI GACnn_n'nnGTC
Cuts at: 0 476 2148
Size: 476 1672
AlwNI CAG_nnn'CTG
Cuts at: 0 976 2148
Size: 976 1172
ApoI r'AATT_y
Cuts at: 0 895 916 950 1048 1370 1556 1900 2148
Size: 895 21 34 98 322 186 344 248
Fragments arranged by size:
895 344 322 248 186 98 34 21
AvaI C'yCGr_G
Cuts at: 0 443 995 1433 2148
Size: 443 552 438 715
Fragments arranged by size:
715 552 443 438
BamHI G'GATC_C
Cuts at: 0 1042 2148
Size: 1042 1106
BanII G_rGCy'C
Cuts at: 0 393 1764 2148
Size: 393 1371 384
Fragments arranged by size:
1371 393 384
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3 37 2148
Size: 3 34 2111
Fragments arranged by size:
2111 34 3
BclI T'GATC_A
Cuts at: 0 222 1404 2148
Size: 222 1182 744
Fragments arranged by size:
1182 744 222
BfrBI ATG'CAT
Cuts at: 0 628 1185 2148
Size: 628 557 963
Fragments arranged by size:
963 628 557
BglI GCCn_nnn'nGGC
Cuts at: 0 1701 2148
Size: 1701 447
BglII A'GATC_T
Cuts at: 0 411 2148
Size: 411 1737
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 466 1558 2148
Size: 466 1092 590
Fragments arranged by size:
1092 590 466
Bpu10I CC'TnA_GC
Cuts at: 0 143 2148
Size: 143 2005
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 650 2143 2148
Size: 650 1493 5
Fragments arranged by size:
1493 650 5
BsaHI Gr'CG_yC
Cuts at: 0 477 2148
Size: 477 1671
BsaWI w'CCGG_w
Cuts at: 0 782 1453 2148
Size: 782 671 695
Fragments arranged by size:
782 695 671
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 95 125 464 494 1525 1555 1787 1817
Size: 95 30 339 30 1031 30 232 30
Cuts at: 1817 2148
Size: 331
Fragments arranged by size:
1031 339 331 232 95 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1825 2148
Size: 1825 323
BseYI C'CCAG_C
Cuts at: 0 157 1763 2148
Size: 157 1606 385
Fragments arranged by size:
1606 385 157
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 287 2148
Size: 287 1861
BsiHKAI G_wGCw'C
Cuts at: 0 19 393 2148
Size: 19 374 1755
Fragments arranged by size:
1755 374 19
BsmI GAATG_Cn'
Cuts at: 0 1122 2148
Size: 1122 1026
BsmBI CGTCTCn'nnnn_
Cuts at: 0 484 2148
Size: 484 1664
Bsp1286I G_dGCh'C
Cuts at: 0 19 393 1764 2148
Size: 19 374 1371 384
Fragments arranged by size:
1371 384 374 19
BspEI T'CCGG_A
Cuts at: 0 782 1453 2148
Size: 782 671 695
Fragments arranged by size:
782 695 671
BspHI T'CATG_A
Cuts at: 0 590 1623 2148
Size: 590 1033 525
Fragments arranged by size:
1033 590 525
BsrDI GCAATG_nn'
Cuts at: 0 1673 2148
Size: 1673 475
BstEII G'GTnAC_C
Cuts at: 0 1521 1893 2148
Size: 1521 372 255
Fragments arranged by size:
1521 372 255
BstYI r'GATC_y
Cuts at: 0 411 813 1042 2148
Size: 411 402 229 1106
Fragments arranged by size:
1106 411 402 229
Bsu36I CC'TnA_GG
Cuts at: 0 697 2148
Size: 697 1451
BtgI C'CryG_G
Cuts at: 0 1696 2148
Size: 1696 452
BtsI GCAGTG_nn'
Cuts at: 0 168 2148
Size: 168 1980
DraI TTT'AAA
Cuts at: 0 453 633 1239 2148
Size: 453 180 606 909
Fragments arranged by size:
909 606 453 180
EaeI y'GGCC_r
Cuts at: 0 424 2148
Size: 424 1724
EarI CTCTTCn'nnn_
Cuts at: 0 398 724 1159 1794 2148
Size: 398 326 435 635 354
Fragments arranged by size:
635 435 398 354 326
EcoICRI GAG'CTC
Cuts at: 0 391 2148
Size: 391 1757
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 400 466 632 1558 2148
Size: 400 66 166 926 590
Fragments arranged by size:
926 590 400 166 66
EcoO109I rG'GnC_Cy
Cuts at: 0 186 1567 2148
Size: 186 1381 581
Fragments arranged by size:
1381 581 186
EcoRI G'AATT_C
Cuts at: 0 895 950 2148
Size: 895 55 1198
Fragments arranged by size:
1198 895 55
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 970 2148
Size: 970 1178
HaeII r_GCGC'y
Cuts at: 0 526 2148
Size: 526 1622
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 462 494 547 579 1194 1226 2148
Size: 462 32 53 32 615 32 922
Fragments arranged by size:
922 615 462 53 32 32 32
HindIII A'AGCT_T
Cuts at: 0 1918 2072 2148
Size: 1918 154 76
Fragments arranged by size:
1918 154 76
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 942 1445 2117 2148
Size: 942 503 672 31
Fragments arranged by size:
942 672 503 31
MscI TGG'CCA
Cuts at: 0 426 2148
Size: 426 1722
MslI CAynn'nnrTG
Cuts at: 0 131 623 2148
Size: 131 492 1525
Fragments arranged by size:
1525 492 131
MspA1I CmG'CkG
Cuts at: 0 228 1501 1767 2148
Size: 228 1273 266 381
Fragments arranged by size:
1273 381 266 228
NdeI CA'TA_TG
Cuts at: 0 2013 2148
Size: 2013 135
NsiI A_TGCA'T
Cuts at: 0 630 1187 2148
Size: 630 557 961
Fragments arranged by size:
961 630 557
NspI r_CATG'y
Cuts at: 0 850 1329 1720 2148
Size: 850 479 391 428
Fragments arranged by size:
850 479 428 391
PasI CC'CwG_GG
Cuts at: 0 970 2148
Size: 970 1178
PciI A'CATG_T
Cuts at: 0 846 1716 2148
Size: 846 870 432
Fragments arranged by size:
870 846 432
PflMI CCAn_nnn'nTGG
Cuts at: 0 976 1711 2148
Size: 976 735 437
Fragments arranged by size:
976 735 437
PfoI T'CCnGG_A
Cuts at: 0 246 2148
Size: 246 1902
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 122 154 2148
Size: 122 32 1994
Fragments arranged by size:
1994 122 32
PpuMI rG'GwC_Cy
Cuts at: 0 186 2148
Size: 186 1962
PshAI GACnn'nnGTC
Cuts at: 0 1719 2148
Size: 1719 429
PspXI vC'TCGA_Gb
Cuts at: 0 1433 2148
Size: 1433 715
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 342 374 2148
Size: 342 32 1774
Fragments arranged by size:
1774 342 32
PstI C_TGCA'G
Cuts at: 0 810 2148
Size: 810 1338
PvuII CAG'CTG
Cuts at: 0 228 1501 1767 2148
Size: 228 1273 266 381
Fragments arranged by size:
1273 381 266 228
SacI G_AGCT'C
Cuts at: 0 393 2148
Size: 393 1755
SapI GCTCTTCn'nnn_
Cuts at: 0 398 2148
Size: 398 1750
ScaI AGT'ACT
Cuts at: 0 421 2148
Size: 421 1727
SfcI C'TryA_G
Cuts at: 0 806 1935 2148
Size: 806 1129 213
Fragments arranged by size:
1129 806 213
SmlI C'TyrA_G
Cuts at: 0 180 665 1433 2122 2148
Size: 180 485 768 689 26
Fragments arranged by size:
768 689 485 180 26
StyI C'CwwG_G
Cuts at: 0 189 1342 2148
Size: 189 1153 806
Fragments arranged by size:
1153 806 189
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1057 2148
Size: 1057 1091
TatI w'GTAC_w
Cuts at: 0 349 419 2148
Size: 349 70 1729
Fragments arranged by size:
1729 349 70
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 464 496 1787 1819 2148
Size: 464 32 1291 32 329
Fragments arranged by size:
1291 464 329 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1533 2148
Size: 1533 615
XhoI C'TCGA_G
Cuts at: 0 1433 2148
Size: 1433 715
XmnI GAAnn'nnTTC
Cuts at: 0 653 2148
Size: 653 1495
ZraI GAC'GTC
Cuts at: 0 478 2148
Size: 478 1670
Enzymes that do cut:
AatII AccI AcuI AflII AflIII AhdI AlwNI ApoI
AvaI BamHI BanII BcgI BclI BfrBI BglI BglII
BpmI Bpu10I BpuEI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BsrDI
BstEII BstYI Bsu36I BtgI BtsI DraI EaeI EarI
EcoICRI Eco57MI EcoO109I EcoRI FalI HaeII Hin4I HindIII
MmeI MscI MslI MspA1I NdeI NsiI NspI PasI
PciI PflMI PfoI PpiI PpuMI PshAI PspXI PsrI
PstI PvuII SacI SapI ScaI SfcI SmlI StyI
TaqII TatI TstI XcmI XhoI XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AgeI AleI AlfI AloI
ApaI ApaLI AscI AseI AsiSI AvrII BaeI BanI
BbeI BbsI BbvCI BciVI BlpI Bme1580I BmgBI BmrI
BmtI BplI BsaI BsaAI BsaBI BsiEI BsiWI BspMI
BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstXI
BstZ17I BtgZI ClaI CspCI DraIII DrdI EagI EciI
EcoNI EcoRV FseI FspI FspAI HincII HpaI KasI
KpnI MfeI MluI NaeI NarI NcoI NgoMIV NheI
NotI NruI PacI PmeI PmlI PsiI PspOMI PvuI
RsrII SacII SalI SanDI SbfI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
StuI SwaI Tth111I XbaI XmaI