Restriction map for
pF1KB3095
(Linear) (Six-base) MAPSORT of: KIBB3095.seq Check: 9691 from: 1 to: 981
KIBB3095.orf
With 182 enzymes: SgfI *
March 3, 2008 10:56 ..
AatII G_ACGT'C
Cuts at: 0 939 981
Size: 939 42
AccI GT'mk_AC
Cuts at: 0 202 981
Size: 202 779
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 110 981
Size: 110 871
AflII C'TTAA_G
Cuts at: 0 357 981
Size: 357 624
AhdI GACnn_n'nnGTC
Cuts at: 0 940 981
Size: 940 41
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 948 980 981
Size: 948 32 1
Fragments arranged by size:
948 32 1
ApoI r'AATT_y
Cuts at: 0 176 697 763 927 981
Size: 176 521 66 164 54
Fragments arranged by size:
521 176 164 66 54
AvaI C'yCGr_G
Cuts at: 0 122 981
Size: 122 859
BanII G_rGCy'C
Cuts at: 0 738 981
Size: 738 243
BbsI GAAGACnn'nnnn_
Cuts at: 0 451 981
Size: 451 530
BglII A'GATC_T
Cuts at: 0 128 500 981
Size: 128 372 481
Fragments arranged by size:
481 372 128
BmtI G_CTAG'C
Cuts at: 0 383 981
Size: 383 598
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 67 981
Size: 67 914
BsaI GGTCTCn'nnnn_
Cuts at: 0 561 981
Size: 561 420
BsaHI Gr'CG_yC
Cuts at: 0 936 981
Size: 936 45
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 260 290 671 701 981
Size: 260 30 381 30 280
Fragments arranged by size:
381 280 260 30 30
BseYI C'CCAG_C
Cuts at: 0 641 981
Size: 641 340
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 100 981
Size: 100 881
BsiHKAI G_wGCw'C
Cuts at: 0 738 981
Size: 738 243
BsmI GAATG_Cn'
Cuts at: 0 418 981
Size: 418 563
Bsp1286I G_dGCh'C
Cuts at: 0 738 981
Size: 738 243
BspHI T'CATG_A
Cuts at: 0 711 981
Size: 711 270
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 542 981
Size: 542 439
BstEII G'GTnAC_C
Cuts at: 0 786 981
Size: 786 195
BstXI CCAn_nnnn'nTGG
Cuts at: 0 919 981
Size: 919 62
BstYI r'GATC_y
Cuts at: 0 128 500 981
Size: 128 372 481
Fragments arranged by size:
481 372 128
BstZ17I GTA'TAC
Cuts at: 0 203 981
Size: 203 778
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 19 956 981
Size: 19 937 25
Fragments arranged by size:
937 25 19
EcoICRI GAG'CTC
Cuts at: 0 736 981
Size: 736 245
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 67 110 981
Size: 67 43 871
Fragments arranged by size:
871 67 43
EcoNI CCTnn'n_nnAGG
Cuts at: 0 587 981
Size: 587 394
EcoRI G'AATT_C
Cuts at: 0 927 981
Size: 927 54
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 523 555 926 958 981
Size: 523 32 371 32 23
Fragments arranged by size:
523 371 32 32 23
MslI CAynn'nnrTG
Cuts at: 0 374 981
Size: 374 607
MspA1I CmG'CkG
Cuts at: 0 171 981
Size: 171 810
NheI G'CTAG_C
Cuts at: 0 379 981
Size: 379 602
PfoI T'CCnGG_A
Cuts at: 0 66 981
Size: 66 915
PsiI TTA'TAA
Cuts at: 0 326 981
Size: 326 655
SacI G_AGCT'C
Cuts at: 0 738 981
Size: 738 243
SfcI C'TryA_G
Cuts at: 0 476 981
Size: 476 505
SmlI C'TyrA_G
Cuts at: 0 357 981
Size: 357 624
SspI AAT'ATT
Cuts at: 0 432 981
Size: 432 549
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 62 94 260 292 981
Size: 62 32 166 32 689
Fragments arranged by size:
689 166 62 32 32
XmnI GAAnn'nnTTC
Cuts at: 0 448 502 981
Size: 448 54 479
Fragments arranged by size:
479 448 54
ZraI GAC'GTC
Cuts at: 0 937 981
Size: 937 44
Enzymes that do cut:
AatII AccI AcuI AflII AhdI AloI ApoI AvaI
BanII BbsI BglII BmtI BpmI BsaI BsaHI BsaXI
BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI BstEII
BstXI BstYI BstZ17I EciI EcoICRI Eco57MI EcoNI EcoRI
Hin4I MslI MspA1I NheI PfoI PsiI SacI SfcI
SmlI SspI TstI XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflIII AgeI AleI AlfI
AlwNI ApaI ApaLI AscI AseI AsiSI AvrII BaeI
BamHI BanI BbeI BbvCI BcgI BciVI BclI BfrBI
BglI BlpI Bme1580I BmgBI BmrI BplI Bpu10I BpuEI
BsaAI BsaBI BsaWI BseRI BsiEI BsiWI BsmBI BspEI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EcoO109I EcoRV FalI FseI
FspI FspAI HaeII HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI NaeI NarI NcoI NdeI
NgoMIV NotI NruI NsiI NspI PacI PasI PciI
PflMI PmeI PmlI PpiI PpuMI PshAI PspOMI PspXI
PsrI PstI PvuI PvuII RsrII SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI StuI StyI SwaI
TaqII TatI Tth111I XbaI XcmI XhoI XmaI