(Linear) (Six-base) MAPSORT of: KIBB3107.seq  Check: 5618  from: 1  to: 495



KIBB3107.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:23  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     442     495 
   Size:        442      53
 
AfeI AGC'GCT

Cuts at:      0     264     495 
   Size:        264     231
 
AflII C'TTAA_G

Cuts at:      0     328     495 
   Size:        328     167
 
ApoI r'AATT_y

Cuts at:      0     450     495 
   Size:        450      45
 
AvaI C'yCGr_G

Cuts at:      0     369     495 
   Size:        369     126
 
BanI G'GyrC_C

Cuts at:      0     137     495 
   Size:        137     358
 
BmgBI CAC'GTC

Cuts at:      0     185     495 
   Size:        185     310
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     127     495 
   Size:        127     368
 
BseYI C'CCAG_C

Cuts at:      0     168     495 
   Size:        168     327
 
BsiEI CG_ry'CG

Cuts at:      0      44     495 
   Size:         44     451
 
BsiHKAI G_wGCw'C

Cuts at:      0     151     495 
   Size:        151     344
 
Bsp1286I G_dGCh'C

Cuts at:      0     151     495 
   Size:        151     344
 
BspHI T'CATG_A

Cuts at:      0     299     495 
   Size:        299     196
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     358     495 
   Size:        358     137
 
BsrDI GCAATG_nn'

Cuts at:      0     321     495 
   Size:        321     174
 
BsrFI r'CCGG_y

Cuts at:      0      43     134     495 
   Size:         43      91     361

  Fragments arranged by size: 

                 361      91      43
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     266     495 
   Size:        266     229
 
BtgI C'CryG_G

Cuts at:      0     113     495 
   Size:        113     382
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     487     495 
   Size:        487       8
 
EaeI y'GGCC_r

Cuts at:      0      41     441     495 
   Size:         41     400      54

  Fragments arranged by size: 

                 400      54      41
 
EagI C'GGCC_G

Cuts at:      0      41     495 
   Size:         41     454
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     442     495 
   Size:        442      53
 
EcoRI G'AATT_C

Cuts at:      0     450     495 
   Size:        450      45
 
FspI TGC'GCA

Cuts at:      0      69     495 
   Size:         69     426
 
HaeII r_GCGC'y

Cuts at:      0     266     495 
   Size:        266     229
 
MscI TGG'CCA

Cuts at:      0     443     495 
   Size:        443      52
 
MspA1I CmG'CkG

Cuts at:      0     168     495 
   Size:        168     327
 
NaeI GCC'GGC

Cuts at:      0      45     495 
   Size:         45     450
 
NcoI C'CATG_G

Cuts at:      0     113     495 
   Size:        113     382
 
NgoMIV G'CCGG_C

Cuts at:      0      43     495 
   Size:         43     452
 
NspI r_CATG'y

Cuts at:      0     344     495 
   Size:        344     151
 
PfoI T'CCnGG_A

Cuts at:      0      55     495 
   Size:         55     440
 
PvuII CAG'CTG

Cuts at:      0     168     495 
   Size:        168     327
 
RsrII CG'GwC_CG

Cuts at:      0      93     495 
   Size:         93     402
 
SfcI C'TryA_G

Cuts at:      0     425     495 
   Size:        425      70
 
SmaI CCC'GGG

Cuts at:      0     371     495 
   Size:        371     124
 
SmlI C'TyrA_G

Cuts at:      0     142     328     495 
   Size:        142     186     167

  Fragments arranged by size: 

                 186     167     142
 
StyI C'CwwG_G

Cuts at:      0     113     495 
   Size:        113     382
 
XmaI C'CCGG_G

Cuts at:      0     369     495 
   Size:        369     126
 

 Enzymes that do cut:

     AcuI     AfeI    AflII     ApoI     AvaI     BanI    BmgBI    BpuEI
    BseYI    BsiEI  BsiHKAI Bsp1286I    BspHI    BspMI    BsrDI    BsrFI
   BstAPI     BtgI     DrdI     EaeI     EagI  Eco57MI    EcoRI     FspI
    HaeII     MscI   MspA1I     NaeI     NcoI   NgoMIV     NspI     PfoI
    PvuII    RsrII     SfcI     SmaI     SmlI     StyI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI   AflIII     AgeI     AhdI
     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI    BanII     BbeI     BbsI    BbvCI
     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I
     BmrI     BmtI     BplI     BpmI   Bpu10I     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI     BsgI    BsiWI     BsmI    BsmBI
    BspEI    BsrBI    BsrGI   BssHII    BssSI    BstBI   BstEII    BstXI
    BstYI  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     EarI     EciI  EcoICRI    EcoNI EcoO109I    EcoRV     FalI
     FseI    FspAI    Hin4I   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MslI     NarI     NdeI     NheI     NotI
     NruI     NsiI     PacI     PasI     PciI    PflMI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     SwaI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmnI     ZraI