Restriction map for
pF1KB3109
(Linear) (Six-base) MAPSORT of: KIBB3109.seq Check: 3533 from: 1 to: 5358
KIBB3109.orf
With 182 enzymes: SgfI *
February 28, 2007 11:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 3346 5358
Size: 3346 2012
AatII G_ACGT'C
Cuts at: 0 1614 5358
Size: 1614 3744
AccI GT'mk_AC
Cuts at: 0 1521 5358
Size: 1521 3837
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 623 992 4388 4418 4457 4666 5147 5358
Size: 623 369 3396 30 39 209 481 211
Fragments arranged by size:
3396 623 481 369 211 209 39 30
AflII C'TTAA_G
Cuts at: 0 322 1497 4972 5358
Size: 322 1175 3475 386
Fragments arranged by size:
3475 1175 386 322
AflIII A'CryG_T
Cuts at: 0 1195 4928 5358
Size: 1195 3733 430
Fragments arranged by size:
3733 1195 430
AhdI GACnn_n'nnGTC
Cuts at: 0 428 5358
Size: 428 4930
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 4709 5358
Size: 4709 649
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 58 90 4906 4938 5358
Size: 58 32 4816 32 420
Fragments arranged by size:
4816 420 58 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1479 2189 2638 2795 4177 4875 4909 5358
Size: 1479 710 449 157 1382 698 34 449
Fragments arranged by size:
1479 1382 710 698 449 449 157 34
ApaLI G'TGCA_C
Cuts at: 0 2417 3492 5358
Size: 2417 1075 1866
Fragments arranged by size:
2417 1866 1075
ApoI r'AATT_y
Cuts at: 0 388 675 1226 3919 5106 5358
Size: 388 287 551 2693 1187 252
Fragments arranged by size:
2693 1187 551 388 287 252
AvaI C'yCGr_G
Cuts at: 0 50 70 3507 5358
Size: 50 20 3437 1851
Fragments arranged by size:
3437 1851 50 20
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2199 2232 2838 2871 3036 3069 5358
Size: 2199 33 606 33 165 33 2289
Fragments arranged by size:
2289 2199 606 165 33 33 33
BanI G'GyrC_C
Cuts at: 0 3100 3946 5358
Size: 3100 846 1412
Fragments arranged by size:
3100 1412 846
BanII G_rGCy'C
Cuts at: 0 51 2089 3712 5358
Size: 51 2038 1623 1646
Fragments arranged by size:
2038 1646 1623 51
BbsI GAAGACnn'nnnn_
Cuts at: 0 3160 3507 4076 4445 4459 4859 5068 5249
Size: 3160 347 569 369 14 400 209 181
Cuts at: 5249 5358
Size: 109
Fragments arranged by size:
3160 569 400 369 347 209 181 109
14
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 791 825 2536 2570 3055 3089 5358
Size: 791 34 1711 34 485 34 2269
Fragments arranged by size:
2269 1711 791 485 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 662 2839 2875 3037 5358
Size: 662 2177 36 162 2321
Fragments arranged by size:
2321 2177 662 162 36
BclI T'GATC_A
Cuts at: 0 3563 5358
Size: 3563 1795
Bme1580I G_kGCm'C
Cuts at: 0 843 2421 2573 3103 3496 4362 5358
Size: 843 1578 152 530 393 866 996
Fragments arranged by size:
1578 996 866 843 530 393 152
BmgBI CAC'GTC
Cuts at: 0 164 2079 3378 4074 5358
Size: 164 1915 1299 696 1284
Fragments arranged by size:
1915 1299 1284 696 164
BmrI ACTGGGnnnn_n'
Cuts at: 0 1500 1878 2438 2583 2626 2648 2990 4089
Size: 1500 378 560 145 43 22 342 1099
Cuts at: 4089 5358
Size: 1269
Fragments arranged by size:
1500 1269 1099 560 378 342 145 43
22
BmtI G_CTAG'C
Cuts at: 0 22 5358
Size: 22 5336
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1421 1772 3622 5358
Size: 1421 351 1850 1736
Fragments arranged by size:
1850 1736 1421 351
Bpu10I CC'TnA_GC
Cuts at: 0 62 5358
Size: 62 5296
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 717 1965 2977 3779 4006 4649 5177 5258
Size: 717 1248 1012 802 227 643 528 81
Cuts at: 5258 5358
Size: 100
Fragments arranged by size:
1248 1012 802 717 643 528 227 100
81
BsaI GGTCTCn'nnnn_
Cuts at: 0 253 957 1264 3362 4398 5358
Size: 253 704 307 2098 1036 960
Fragments arranged by size:
2098 1036 960 704 307 253
BsaAI yAC'GTr
Cuts at: 0 1196 4642 5358
Size: 1196 3446 716
Fragments arranged by size:
3446 1196 716
BsaBI GATnn'nnATC
Cuts at: 0 251 757 5358
Size: 251 506 4601
Fragments arranged by size:
4601 506 251
BsaHI Gr'CG_yC
Cuts at: 0 1611 2778 3421 5358
Size: 1611 1167 643 1937
Fragments arranged by size:
1937 1611 1167 643
BsaWI w'CCGG_w
Cuts at: 0 1733 5358
Size: 1733 3625
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 294 324 2156 2186 5358
Size: 294 30 1832 30 3172
Fragments arranged by size:
3172 1832 294 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 2055 4009 4129 4507 5358
Size: 2055 1954 120 378 851
Fragments arranged by size:
2055 1954 851 378 120
BseYI C'CCAG_C
Cuts at: 0 1203 4934 5358
Size: 1203 3731 424
Fragments arranged by size:
3731 1203 424
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 63 157 918 1912 3510 5066 5358
Size: 63 94 761 994 1598 1556 292
Fragments arranged by size:
1598 1556 994 761 292 94 63
BsiEI CG_ry'CG
Cuts at: 0 135 457 2005 5358
Size: 135 322 1548 3353
Fragments arranged by size:
3353 1548 322 135
BsiHKAI G_wGCw'C
Cuts at: 0 141 1490 1588 2421 3116 3496 5358
Size: 141 1349 98 833 695 380 1862
Fragments arranged by size:
1862 1349 833 695 380 141 98
BsmI GAATG_Cn'
Cuts at: 0 2326 4011 5358
Size: 2326 1685 1347
Fragments arranged by size:
2326 1685 1347
BsmBI CGTCTCn'nnnn_
Cuts at: 0 3412 5358
Size: 3412 1946
Bsp1286I G_dGCh'C
Cuts at: 0 51 141 843 1490 1588 2089 2421 2573
Size: 51 90 702 647 98 501 332 152
Cuts at: 2573 3103 3116 3496 3712 4362 5358
Size: 530 13 380 216 650 996
Fragments arranged by size:
996 702 650 647 530 501 380 332
216 152 98 90 51 13
BspHI T'CATG_A
Cuts at: 0 255 4052 5358
Size: 255 3797 1306
Fragments arranged by size:
3797 1306 255
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 975 2413 3346 4883 5358
Size: 975 1438 933 1537 475
Fragments arranged by size:
1537 1438 975 933 475
BsrBI CCG'CTC
Cuts at: 0 1716 5358
Size: 1716 3642
BsrDI GCAATG_nn'
Cuts at: 0 1017 1262 1509 2613 3264 3294 3566 4494
Size: 1017 245 247 1104 651 30 272 928
Cuts at: 4494 4700 5358
Size: 206 658
Fragments arranged by size:
1104 1017 928 658 651 272 247 245
206 30
BsrFI r'CCGG_y
Cuts at: 0 1592 1756 2004 2381 3035 5358
Size: 1592 164 248 377 654 2323
Fragments arranged by size:
2323 1592 654 377 248 164
BssHII G'CGCG_C
Cuts at: 0 57 5358
Size: 57 5301
BstAPI GCAn_nnn'nTGC
Cuts at: 0 3347 3533 5358
Size: 3347 186 1825
Fragments arranged by size:
3347 1825 186
BstEII G'GTnAC_C
Cuts at: 0 153 2175 5358
Size: 153 2022 3183
Fragments arranged by size:
3183 2022 153
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1490 2608 3852 4338 5358
Size: 1490 1118 1244 486 1020
Fragments arranged by size:
1490 1244 1118 1020 486
BstYI r'GATC_y
Cuts at: 0 246 630 651 1170 1350 2479 2805 5358
Size: 246 384 21 519 180 1129 326 2553
Fragments arranged by size:
2553 1129 519 384 326 246 180 21
Bsu36I CC'TnA_GG
Cuts at: 0 2330 2877 5358
Size: 2330 547 2481
Fragments arranged by size:
2481 2330 547
BtgI C'CryG_G
Cuts at: 0 113 1058 1106 1820 2150 2669 3440 4331
Size: 113 945 48 714 330 519 771 891
Cuts at: 4331 5358
Size: 1027
Fragments arranged by size:
1027 945 891 771 714 519 330 113
48
BtsI GCAGTG_nn'
Cuts at: 0 888 1132 1350 1431 2554 2736 3310 4769
Size: 888 244 218 81 1123 182 574 1459
Cuts at: 4769 5358
Size: 589
Fragments arranged by size:
1459 1123 888 589 574 244 218 182
81
ClaI AT'CG_AT
Cuts at: 0 2102 5358
Size: 2102 3256
DraIII CAC_nnn'GTG
Cuts at: 0 3430 3484 5358
Size: 3430 54 1874
Fragments arranged by size:
3430 1874 54
DrdI GACnn_nn'nnGTC
Cuts at: 0 4348 5358
Size: 4348 1010
EaeI y'GGCC_r
Cuts at: 0 132 1055 1269 2002 2511 2610 3224 5358
Size: 132 923 214 733 509 99 614 2134
Fragments arranged by size:
2134 923 733 614 509 214 132 99
EagI C'GGCC_G
Cuts at: 0 132 2002 5358
Size: 132 1870 3356
Fragments arranged by size:
3356 1870 132
EarI CTCTTCn'nnn_
Cuts at: 0 3364 5123 5358
Size: 3364 1759 235
Fragments arranged by size:
3364 1759 235
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 623 992 1421 1772 3622 4388 4418 4457
Size: 623 369 429 351 1850 766 30 39
Cuts at: 4457 4666 5147 5358
Size: 209 481 211
Fragments arranged by size:
1850 766 623 481 429 369 351 211
209 39 30
EcoNI CCTnn'n_nnAGG
Cuts at: 0 971 3064 5358
Size: 971 2093 2294
Fragments arranged by size:
2294 2093 971
EcoO109I rG'GnC_Cy
Cuts at: 0 1693 4208 4279 5358
Size: 1693 2515 71 1079
Fragments arranged by size:
2515 1693 1079 71
EcoRI G'AATT_C
Cuts at: 0 388 5358
Size: 388 4970
FspI TGC'GCA
Cuts at: 0 90 5358
Size: 90 5268
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 252 284 414 446 1596 1628 1976 2008
Size: 252 32 130 32 1150 32 348 32
Cuts at: 2008 2091 2123 2860 2892 4075 4107 5358
Size: 83 32 737 32 1183 32 1251
Fragments arranged by size:
1251 1183 1150 737 348 252 130 83
32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 1522 5358
Size: 1522 3836
HindIII A'AGCT_T
Cuts at: 0 145 5358
Size: 145 5213
MluI A'CGCG_T
Cuts at: 0 4928 5358
Size: 4928 430
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 393 1936 2024 2376 5358
Size: 393 1543 88 352 2982
Fragments arranged by size:
2982 1543 393 352 88
MscI TGG'CCA
Cuts at: 0 1057 1271 2513 2612 3226 5358
Size: 1057 214 1242 99 614 2132
Fragments arranged by size:
2132 1242 1057 614 214 99
MslI CAynn'nnrTG
Cuts at: 0 1310 1888 2618 2681 5358
Size: 1310 578 730 63 2677
Fragments arranged by size:
2677 1310 730 578 63
MspA1I CmG'CkG
Cuts at: 0 101 436 1073 1207 3200 3222 4177 5137
Size: 101 335 637 134 1993 22 955 960
Cuts at: 5137 5358
Size: 221
Fragments arranged by size:
1993 960 955 637 335 221 134 101
22
NaeI GCC'GGC
Cuts at: 0 1594 1758 2383 5358
Size: 1594 164 625 2975
Fragments arranged by size:
2975 1594 625 164
NcoI C'CATG_G
Cuts at: 0 1106 1820 2669 4331 5358
Size: 1106 714 849 1662 1027
Fragments arranged by size:
1662 1106 1027 849 714
NdeI CA'TA_TG
Cuts at: 0 232 5358
Size: 232 5126
NgoMIV G'CCGG_C
Cuts at: 0 1592 1756 2381 5358
Size: 1592 164 625 2977
Fragments arranged by size:
2977 1592 625 164
NheI G'CTAG_C
Cuts at: 0 18 5358
Size: 18 5340
NspI r_CATG'y
Cuts at: 0 897 3318 5354 5358
Size: 897 2421 2036 4
Fragments arranged by size:
2421 2036 897 4
PasI CC'CwG_GG
Cuts at: 0 3407 4204 5358
Size: 3407 797 1154
Fragments arranged by size:
3407 1154 797
PflMI CCAn_nnn'nTGG
Cuts at: 0 2638 3215 5358
Size: 2638 577 2143
Fragments arranged by size:
2638 2143 577
PfoI T'CCnGG_A
Cuts at: 0 748 1398 1727 1744 2841 3599 5358
Size: 748 650 329 17 1097 758 1759
Fragments arranged by size:
1759 1097 758 748 650 329 17
PstI C_TGCA'G
Cuts at: 0 3343 4249 4776 5358
Size: 3343 906 527 582
Fragments arranged by size:
3343 906 582 527
PvuI CG_AT'CG
Cuts at: 0 457 5358
Size: 457 4901
PvuII CAG'CTG
Cuts at: 0 101 436 1207 3222 5137 5358
Size: 101 335 771 2015 1915 221
Fragments arranged by size:
2015 1915 771 335 221 101
SalI G'TCGA_C
Cuts at: 0 1520 5358
Size: 1520 3838
SexAI A'CCwGG_T
Cuts at: 0 1632 1955 5358
Size: 1632 323 3403
Fragments arranged by size:
3403 1632 323
SfcI C'TryA_G
Cuts at: 0 3339 3862 4245 4772 5358
Size: 3339 523 383 527 586
Fragments arranged by size:
3339 586 527 523 383
SmlI C'TyrA_G
Cuts at: 0 322 696 1497 1980 2992 3794 3985 4664
Size: 322 374 801 483 1012 802 191 679
Cuts at: 4664 4972 5192 5273 5358
Size: 308 220 81 85
Fragments arranged by size:
1012 802 801 679 483 374 322 308
220 191 85 81
SphI G_CATG'C
Cuts at: 0 3318 5358
Size: 3318 2040
StuI AGG'CCT
Cuts at: 0 514 2309 3616 5358
Size: 514 1795 1307 1742
Fragments arranged by size:
1795 1742 1307 514
StyI C'CwwG_G
Cuts at: 0 664 911 958 1106 1539 1820 2669 3611
Size: 664 247 47 148 433 281 849 942
Cuts at: 3611 3950 4331 5358
Size: 339 381 1027
Fragments arranged by size:
1027 942 849 664 433 381 339 281
247 148 47
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1166 5358
Size: 1166 4192
TatI w'GTAC_w
Cuts at: 0 2057 5358
Size: 2057 3301
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2156 2188 3581 3613 5358
Size: 2156 32 1393 32 1745
Fragments arranged by size:
2156 1745 1393 32 32
XbaI T'CTAG_A
Cuts at: 0 2217 5358
Size: 2217 3141
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 3316 4032 4473 5358
Size: 3316 716 441 885
Fragments arranged by size:
3316 885 716 441
XmnI GAAnn'nnTTC
Cuts at: 0 2198 4442 4856 5358
Size: 2198 2244 414 502
Fragments arranged by size:
2244 2198 502 414
ZraI GAC'GTC
Cuts at: 0 1612 5358
Size: 1612 3746
Enzymes that do cut:
AarI AatII AccI AcuI AflII AflIII AhdI AlfI
AloI AlwNI ApaLI ApoI AvaI BaeI BanI BanII
BbsI BcgI BciVI BclI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssHII BstAPI
BstEII BstXI BstYI Bsu36I BtgI BtsI ClaI DraIII
DrdI EaeI EagI EarI Eco57MI EcoNI EcoO109I EcoRI
FspI Hin4I HincII HindIII MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NspI PasI
PflMI PfoI PstI PvuI PvuII SalI SexAI SfcI
SmlI SphI StuI StyI TaqII TatI TstI XbaI
XcmI XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AgeI AleI ApaI AscI AseI
AsiSI AvrII BamHI BbeI BbvCI BfrBI BglI BglII
BlpI BplI BsiWI BspEI BsrGI BssSI BstBI BstZ17I
BtgZI CspCI DraI EciI EcoICRI EcoRV FalI FseI
FspAI HaeII HpaI KasI KpnI MfeI NarI NotI
NruI NsiI PacI PciI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PsrI RsrII SacI SacII
SanDI SapI SbfI ScaI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SrfI SspI SwaI Tth111I XhoI
XmaI