Restriction map for
pF1KB3111
(Linear) (Six-base) MAPSORT of: KIBB3111.seq Check: 3695 from: 1 to: 1083
KIBB3111.orf
With 182 enzymes: SgfI *
March 3, 2008 10:56 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 559 1083
Size: 559 524
AccI GT'mk_AC
Cuts at: 0 282 1083
Size: 282 801
AgeI A'CCGG_T
Cuts at: 0 7 1083
Size: 7 1076
AhdI GACnn_n'nnGTC
Cuts at: 0 289 1083
Size: 289 794
AleI CACnn'nnGTG
Cuts at: 0 10 1083
Size: 10 1073
ApaLI G'TGCA_C
Cuts at: 0 131 1083
Size: 131 952
BanI G'GyrC_C
Cuts at: 0 907 1083
Size: 907 176
BanII G_rGCy'C
Cuts at: 0 387 1083
Size: 387 696
BbeI G_GCGC'C
Cuts at: 0 911 1083
Size: 911 172
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 66 100 1083
Size: 66 34 983
Fragments arranged by size:
983 66 34
BclI T'GATC_A
Cuts at: 0 221 1083
Size: 221 862
BfrBI ATG'CAT
Cuts at: 0 60 835 1083
Size: 60 775 248
Fragments arranged by size:
775 248 60
BglII A'GATC_T
Cuts at: 0 482 1083
Size: 482 601
Bme1580I G_kGCm'C
Cuts at: 0 135 1083
Size: 135 948
BmrI ACTGGGnnnn_n'
Cuts at: 0 958 1083
Size: 958 125
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 673 788 1083
Size: 673 115 295
Fragments arranged by size:
673 295 115
Bpu10I CC'TnA_GC
Cuts at: 0 386 1083
Size: 386 697
BsaAI yAC'GTr
Cuts at: 0 136 1083
Size: 136 947
BsaHI Gr'CG_yC
Cuts at: 0 900 908 1083
Size: 900 8 175
Fragments arranged by size:
900 175 8
BsaWI w'CCGG_w
Cuts at: 0 7 555 775 1083
Size: 7 548 220 308
Fragments arranged by size:
548 308 220 7
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 89 119 1083
Size: 89 30 964
Fragments arranged by size:
964 89 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 136 1083
Size: 136 947
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 709 1083
Size: 709 374
BsiHKAI G_wGCw'C
Cuts at: 0 135 1083
Size: 135 948
BsmI GAATG_Cn'
Cuts at: 0 449 1083
Size: 449 634
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1054 1083
Size: 1054 29
Bsp1286I G_dGCh'C
Cuts at: 0 135 387 1083
Size: 135 252 696
Fragments arranged by size:
696 252 135
BspHI T'CATG_A
Cuts at: 0 855 1083
Size: 855 228
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 559 978 1083
Size: 559 419 105
Fragments arranged by size:
559 419 105
BsrBI CCG'CTC
Cuts at: 0 429 1083
Size: 429 654
BsrDI GCAATG_nn'
Cuts at: 0 743 1083
Size: 743 340
BsrFI r'CCGG_y
Cuts at: 0 7 533 1083
Size: 7 526 550
Fragments arranged by size:
550 526 7
BstEII G'GTnAC_C
Cuts at: 0 291 990 1083
Size: 291 699 93
Fragments arranged by size:
699 291 93
BstYI r'GATC_y
Cuts at: 0 482 923 973 1003 1083
Size: 482 441 50 30 80
Fragments arranged by size:
482 441 80 50 30
Bsu36I CC'TnA_GG
Cuts at: 0 298 1083
Size: 298 785
EarI CTCTTCn'nnn_
Cuts at: 0 233 1083
Size: 233 850
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 673 788 1083
Size: 673 115 295
Fragments arranged by size:
673 295 115
HaeII r_GCGC'y
Cuts at: 0 911 1083
Size: 911 172
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 17 49 275 307 341 373 837 869
Size: 17 32 226 32 34 32 464 32
Cuts at: 869 1083
Size: 214
Fragments arranged by size:
464 226 214 34 32 32 32 32
17
KasI G'GCGC_C
Cuts at: 0 907 1083
Size: 907 176
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 479 1083
Size: 479 604
MslI CAynn'nnrTG
Cuts at: 0 10 1083
Size: 10 1073
MspA1I CmG'CkG
Cuts at: 0 274 1083
Size: 274 809
NarI GG'CG_CC
Cuts at: 0 908 1083
Size: 908 175
NsiI A_TGCA'T
Cuts at: 0 62 837 1083
Size: 62 775 246
Fragments arranged by size:
775 246 62
PvuII CAG'CTG
Cuts at: 0 274 1083
Size: 274 809
SfoI GGC'GCC
Cuts at: 0 909 1083
Size: 909 174
SgrAI Cr'CCGG_yG
Cuts at: 0 7 1083
Size: 7 1076
StuI AGG'CCT
Cuts at: 0 673 1083
Size: 673 410
TatI w'GTAC_w
Cuts at: 0 376 1083
Size: 376 707
XbaI T'CTAG_A
Cuts at: 0 666 1083
Size: 666 417
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1020 1083
Size: 1020 63
Enzymes that do cut:
AarI AccI AgeI AhdI AleI ApaLI BanI BanII
BbeI BcgI BclI BfrBI BglII Bme1580I BmrI BpmI
Bpu10I BsaAI BsaHI BsaWI BsaXI BseRI BsgI BsiHKAI
BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BstEII BstYI Bsu36I EarI Eco57MI HaeII Hin4I KasI
MmeI MslI MspA1I NarI NsiI PvuII SfoI SgrAI
StuI TatI XbaI XcmI
Enzymes that do not cut:
AatII Acc65I AclI AcuI AfeI AflII AflIII AlfI
AloI AlwNI ApaI ApoI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BbsI BbvCI BciVI BglI BlpI
BmgBI BmtI BplI BpuEI BsaI BsaBI BseYI BsiEI
BsiWI BspEI BsrGI BssHII BssSI BstAPI BstBI BstXI
BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EciI EcoICRI EcoNI EcoO109I EcoRI
EcoRV FalI FseI FspI FspAI HincII HindIII HpaI
KpnI MfeI MluI MscI NaeI NcoI NdeI NgoMIV
NheI NotI NruI NspI PacI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PsrI PstI PvuI RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfcI SfiI SgfI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StyI
SwaI TaqII TstI Tth111I XhoI XmaI XmnI ZraI