Restriction map for
pF1KB3114
(Linear) (Six-base) MAPSORT of: KIBB3114.seq Check: 2625 from: 1 to: 1635
KIBB3114.orf
With 182 enzymes: SgfI *
July 18, 2007 13:36 ..
AccI GT'mk_AC
Cuts at: 0 149 1635
Size: 149 1486
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 251 293 547 1067 1510 1628 1635
Size: 251 42 254 520 443 118 7
Fragments arranged by size:
520 443 254 251 118 42 7
AflIII A'CryG_T
Cuts at: 0 992 1635
Size: 992 643
AleI CACnn'nnGTG
Cuts at: 0 878 1387 1635
Size: 878 509 248
Fragments arranged by size:
878 509 248
AlwNI CAG_nnn'CTG
Cuts at: 0 90 445 1635
Size: 90 355 1190
Fragments arranged by size:
1190 355 90
ApoI r'AATT_y
Cuts at: 0 349 383 743 927 1635
Size: 349 34 360 184 708
Fragments arranged by size:
708 360 349 184 34
BanII G_rGCy'C
Cuts at: 0 242 1635
Size: 242 1393
BbsI GAAGACnn'nnnn_
Cuts at: 0 222 1598 1635
Size: 222 1376 37
Fragments arranged by size:
1376 222 37
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 475 509 1635
Size: 475 34 1126
Fragments arranged by size:
1126 475 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 219 1635
Size: 219 1416
BmrI ACTGGGnnnn_n'
Cuts at: 0 1236 1635
Size: 1236 399
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1356 1635
Size: 1356 279
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 966 1635
Size: 966 669
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 883 1635
Size: 883 752
BsaAI yAC'GTr
Cuts at: 0 876 1635
Size: 876 759
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1171 1635
Size: 1171 464
BseYI C'CCAG_C
Cuts at: 0 1107 1635
Size: 1107 528
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 902 1541 1635
Size: 902 639 94
Fragments arranged by size:
902 639 94
BsiHKAI G_wGCw'C
Cuts at: 0 242 1635
Size: 242 1393
Bsp1286I G_dGCh'C
Cuts at: 0 242 1635
Size: 242 1393
BspHI T'CATG_A
Cuts at: 0 774 1635
Size: 774 861
BsrDI GCAATG_nn'
Cuts at: 0 758 1400 1635
Size: 758 642 235
Fragments arranged by size:
758 642 235
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1389 1635
Size: 1389 246
BstYI r'GATC_y
Cuts at: 0 951 1635
Size: 951 684
Bsu36I CC'TnA_GG
Cuts at: 0 335 1635
Size: 335 1300
BtgI C'CryG_G
Cuts at: 0 1142 1232 1635
Size: 1142 90 403
Fragments arranged by size:
1142 403 90
BtsI GCAGTG_nn'
Cuts at: 0 1487 1635
Size: 1487 148
ClaI AT'CG_AT
Cuts at: 0 203 1262 1635
Size: 203 1059 373
Fragments arranged by size:
1059 373 203
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 3 38 1217 1252 1635
Size: 3 35 1179 35 383
Fragments arranged by size:
1179 383 35 35 3
DraIII CAC_nnn'GTG
Cuts at: 0 876 1216 1564 1635
Size: 876 340 348 71
Fragments arranged by size:
876 348 340 71
EarI CTCTTCn'nnn_
Cuts at: 0 1295 1486 1635
Size: 1295 191 149
Fragments arranged by size:
1295 191 149
EcoICRI GAG'CTC
Cuts at: 0 240 1635
Size: 240 1395
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 251 293 547 966 1067 1510 1628 1635
Size: 251 42 254 419 101 443 118 7
Fragments arranged by size:
443 419 254 251 118 101 42 7
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1153 1635
Size: 1153 482
EcoO109I rG'GnC_Cy
Cuts at: 0 1451 1635
Size: 1451 184
HaeII r_GCGC'y
Cuts at: 0 90 1635
Size: 90 1545
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 463 495 1210 1242 1635
Size: 463 32 715 32 393
Fragments arranged by size:
715 463 393 32 32
HincII GTy'rAC
Cuts at: 0 141 150 1229 1635
Size: 141 9 1079 406
Fragments arranged by size:
1079 406 141 9
HindIII A'AGCT_T
Cuts at: 0 614 1635
Size: 614 1021
HpaI GTT'AAC
Cuts at: 0 141 1635
Size: 141 1494
MslI CAynn'nnrTG
Cuts at: 0 779 878 1387 1635
Size: 779 99 509 248
Fragments arranged by size:
779 509 248 99
MspA1I CmG'CkG
Cuts at: 0 123 445 673 1635
Size: 123 322 228 962
Fragments arranged by size:
962 322 228 123
NcoI C'CATG_G
Cuts at: 0 1142 1232 1635
Size: 1142 90 403
Fragments arranged by size:
1142 403 90
NdeI CA'TA_TG
Cuts at: 0 419 1635
Size: 419 1216
NspI r_CATG'y
Cuts at: 0 370 996 1635
Size: 370 626 639
Fragments arranged by size:
639 626 370
PciI A'CATG_T
Cuts at: 0 992 1635
Size: 992 643
PflMI CCAn_nnn'nTGG
Cuts at: 0 1118 1635
Size: 1118 517
PfoI T'CCnGG_A
Cuts at: 0 278 1117 1635
Size: 278 839 518
Fragments arranged by size:
839 518 278
PmlI CAC'GTG
Cuts at: 0 876 1635
Size: 876 759
PpuMI rG'GwC_Cy
Cuts at: 0 1451 1635
Size: 1451 184
PstI C_TGCA'G
Cuts at: 0 1172 1635
Size: 1172 463
PvuII CAG'CTG
Cuts at: 0 123 445 673 1635
Size: 123 322 228 962
Fragments arranged by size:
962 322 228 123
SacI G_AGCT'C
Cuts at: 0 242 1635
Size: 242 1393
SalI G'TCGA_C
Cuts at: 0 148 1635
Size: 148 1487
SbfI CC_TGCA'GG
Cuts at: 0 1172 1635
Size: 1172 463
SexAI A'CCwGG_T
Cuts at: 0 1508 1635
Size: 1508 127
SfcI C'TryA_G
Cuts at: 0 986 1168 1635
Size: 986 182 467
Fragments arranged by size:
986 467 182
SmlI C'TyrA_G
Cuts at: 0 862 1635
Size: 862 773
SphI G_CATG'C
Cuts at: 0 370 1635
Size: 370 1265
SspI AAT'ATT
Cuts at: 0 165 1635
Size: 165 1470
StyI C'CwwG_G
Cuts at: 0 1142 1232 1635
Size: 1142 90 403
Fragments arranged by size:
1142 403 90
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1121 1635
Size: 1121 514
TatI w'GTAC_w
Cuts at: 0 638 1635
Size: 638 997
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 374 406 1635
Size: 374 32 1229
Fragments arranged by size:
1229 374 32
Tth111I GACn'n_nGTC
Cuts at: 0 262 822 1635
Size: 262 560 813
Fragments arranged by size:
813 560 262
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1115 1149 1635
Size: 1115 34 486
Fragments arranged by size:
1115 486 34
XmnI GAAnn'nnTTC
Cuts at: 0 1495 1635
Size: 1495 140
Enzymes that do cut:
AccI AcuI AflIII AleI AlwNI ApoI BanII BbsI
BcgI BciVI BmrI BplI BpmI BpuEI BsaAI BseRI
BseYI BsgI BsiHKAI Bsp1286I BspHI BsrDI BstXI BstYI
Bsu36I BtgI BtsI ClaI CspCI DraIII EarI EcoICRI
Eco57MI EcoNI EcoO109I HaeII Hin4I HincII HindIII HpaI
MslI MspA1I NcoI NdeI NspI PciI PflMI PfoI
PmlI PpuMI PstI PvuII SacI SalI SbfI SexAI
SfcI SmlI SphI SspI StyI TaqII TatI TstI
Tth111I XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AlfI AloI ApaI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BanI BbeI BbvCI BclI BfrBI
BglI BglII BlpI Bme1580I BmgBI BmtI Bpu10I BsaI
BsaBI BsaHI BsaWI BsaXI BsiEI BsiWI BsmI BsmBI
BspEI BspMI BsrBI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstZ17I BtgZI DraI DrdI EaeI EagI
EciI EcoRI EcoRV FalI FseI FspI FspAI KasI
KpnI MfeI MluI MmeI MscI NaeI NarI NgoMIV
NheI NotI NruI NsiI PacI PasI PmeI PpiI
PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacII
SanDI SapI ScaI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SrfI StuI SwaI XbaI XhoI XmaI
ZraI