Restriction map for
pF1KB3168
(Linear) (Six-base) MAPSORT of: KIBB3168.seq Check: 6726 from: 1 to: 2370
KIBB3168.orf
With 182 enzymes: SgfI *
February 15, 2010 17:49 ..
AclI AA'CG_TT
Cuts at: 0 1488 2370
Size: 1488 882
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 541 808 2370
Size: 541 267 1562
Fragments arranged by size:
1562 541 267
AflIII A'CryG_T
Cuts at: 0 332 2370
Size: 332 2038
AleI CACnn'nnGTG
Cuts at: 0 2246 2370
Size: 2246 124
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2125 2370
Size: 2125 245
AlwNI CAG_nnn'CTG
Cuts at: 0 880 2370
Size: 880 1490
ApaI G_GGCC'C
Cuts at: 0 1279 2233 2241 2292 2370
Size: 1279 954 8 51 78
Fragments arranged by size:
1279 954 78 51 8
ApoI r'AATT_y
Cuts at: 0 415 451 692 2370
Size: 415 36 241 1678
Fragments arranged by size:
1678 415 241 36
AvaI C'yCGr_G
Cuts at: 0 1303 1313 1552 2150 2370
Size: 1303 10 239 598 220
Fragments arranged by size:
1303 598 239 220 10
BamHI G'GATC_C
Cuts at: 0 1548 2370
Size: 1548 822
BanI G'GyrC_C
Cuts at: 0 1987 2370
Size: 1987 383
BanII G_rGCy'C
Cuts at: 0 1279 1289 1334 1441 1720 2195 2233 2241
Size: 1279 10 45 107 279 475 38 8
Cuts at: 2241 2292 2370
Size: 51 78
Fragments arranged by size:
1279 475 279 107 78 51 45 38
10 8
BbsI GAAGACnn'nnnn_
Cuts at: 0 86 857 2270 2370
Size: 86 771 1413 100
Fragments arranged by size:
1413 771 100 86
BbvCI CC'TCA_GC
Cuts at: 0 1651 2370
Size: 1651 719
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1284 1318 1741 1775 2370
Size: 1284 34 423 34 595
Fragments arranged by size:
1284 595 423 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 2008 2370
Size: 2008 362
BclI T'GATC_A
Cuts at: 0 2301 2370
Size: 2301 69
BfrBI ATG'CAT
Cuts at: 0 315 2370
Size: 315 2055
Bme1580I G_kGCm'C
Cuts at: 0 1103 1133 1279 2233 2241 2292 2370
Size: 1103 30 146 954 8 51 78
Fragments arranged by size:
1103 954 146 78 51 30 8
BmrI ACTGGGnnnn_n'
Cuts at: 0 1370 2370
Size: 1370 1000
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1659 2370
Size: 1659 711
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 59 451 1558 1629 1835 2370
Size: 59 392 1107 71 206 535
Fragments arranged by size:
1107 535 392 206 71 59
Bpu10I CC'TnA_GC
Cuts at: 0 1651 2370
Size: 1651 719
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 373 1760 2118 2370
Size: 373 1387 358 252
Fragments arranged by size:
1387 373 358 252
BsaI GGTCTCn'nnnn_
Cuts at: 0 827 1408 2370
Size: 827 581 962
Fragments arranged by size:
962 827 581
BsaBI GATnn'nnATC
Cuts at: 0 931 2370
Size: 931 1439
BsaHI Gr'CG_yC
Cuts at: 0 1754 2370
Size: 1754 616
BsaWI w'CCGG_w
Cuts at: 0 266 1712 2370
Size: 266 1446 658
Fragments arranged by size:
1446 658 266
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1104 1366 1632 2203 2370
Size: 1104 262 266 571 167
Fragments arranged by size:
1104 571 266 262 167
BseYI C'CCAG_C
Cuts at: 0 947 1133 1199 1393 2370
Size: 947 186 66 194 977
Fragments arranged by size:
977 947 194 186 66
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1452 1486 1766 1839 2370
Size: 1452 34 280 73 531
Fragments arranged by size:
1452 531 280 73 34
BsiHKAI G_wGCw'C
Cuts at: 0 1222 2195 2370
Size: 1222 973 175
Fragments arranged by size:
1222 973 175
BsmI GAATG_Cn'
Cuts at: 0 730 1728 1987 2347 2370
Size: 730 998 259 360 23
Fragments arranged by size:
998 730 360 259 23
Bsp1286I G_dGCh'C
Cuts at: 0 1103 1133 1222 1279 1289 1334 1441 1720
Size: 1103 30 89 57 10 45 107 279
Cuts at: 1720 2195 2233 2241 2292 2370
Size: 475 38 8 51 78
Fragments arranged by size:
1103 475 279 107 89 78 57 51
45 38 30 10 8
BspEI T'CCGG_A
Cuts at: 0 266 1712 2370
Size: 266 1446 658
Fragments arranged by size:
1446 658 266
BspHI T'CATG_A
Cuts at: 0 2058 2370
Size: 2058 312
BsrDI GCAATG_nn'
Cuts at: 0 2319 2370
Size: 2319 51
BsrFI r'CCGG_y
Cuts at: 0 1828 2370
Size: 1828 542
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1613 2342 2370
Size: 1613 729 28
Fragments arranged by size:
1613 729 28
BstEII G'GTnAC_C
Cuts at: 0 780 2370
Size: 780 1590
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1744 2370
Size: 1744 626
BstYI r'GATC_y
Cuts at: 0 876 1548 1601 1695 2370
Size: 876 672 53 94 675
Fragments arranged by size:
876 675 672 94 53
Bsu36I CC'TnA_GG
Cuts at: 0 20 1280 1349 1920 2370
Size: 20 1260 69 571 450
Fragments arranged by size:
1260 571 450 69 20
BtgI C'CryG_G
Cuts at: 0 539 954 1115 2370
Size: 539 415 161 1255
Fragments arranged by size:
1255 539 415 161
BtsI GCAGTG_nn'
Cuts at: 0 1418 2370
Size: 1418 952
DraI TTT'AAA
Cuts at: 0 90 120 414 2370
Size: 90 30 294 1956
Fragments arranged by size:
1956 294 90 30
DraIII CAC_nnn'GTG
Cuts at: 0 1877 2370
Size: 1877 493
EarI CTCTTCn'nnn_
Cuts at: 0 2072 2370
Size: 2072 298
EcoICRI GAG'CTC
Cuts at: 0 2193 2370
Size: 2193 177
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 59 451 541 808 1558 1629 1835 2370
Size: 59 392 90 267 750 71 206 535
Fragments arranged by size:
750 535 392 267 206 90 71 59
EcoNI CCTnn'n_nnAGG
Cuts at: 0 19 2370
Size: 19 2351
EcoO109I rG'GnC_Cy
Cuts at: 0 1276 1307 1366 2230 2237 2289 2370
Size: 1276 31 59 864 7 52 81
Fragments arranged by size:
1276 864 81 59 52 31 7
HaeII r_GCGC'y
Cuts at: 0 283 376 1303 2370
Size: 283 93 927 1067
Fragments arranged by size:
1067 927 283 93
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 96 128 2370
Size: 96 32 2242
Fragments arranged by size:
2242 96 32
HincII GTy'rAC
Cuts at: 0 828 2370
Size: 828 1542
HindIII A'AGCT_T
Cuts at: 0 271 2370
Size: 271 2099
HpaI GTT'AAC
Cuts at: 0 828 2370
Size: 828 1542
MfeI C'AATT_G
Cuts at: 0 303 2370
Size: 303 2067
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 968 2370
Size: 968 1402
MslI CAynn'nnrTG
Cuts at: 0 538 1189 1423 2246 2370
Size: 538 651 234 823 124
Fragments arranged by size:
823 651 538 234 124
MspA1I CmG'CkG
Cuts at: 0 422 2370
Size: 422 1948
NaeI GCC'GGC
Cuts at: 0 1830 2370
Size: 1830 540
NcoI C'CATG_G
Cuts at: 0 539 954 1115 2370
Size: 539 415 161 1255
Fragments arranged by size:
1255 539 415 161
NgoMIV G'CCGG_C
Cuts at: 0 1828 2370
Size: 1828 542
NsiI A_TGCA'T
Cuts at: 0 317 2370
Size: 317 2053
NspI r_CATG'y
Cuts at: 0 319 336 2370
Size: 319 17 2034
Fragments arranged by size:
2034 319 17
PasI CC'CwG_GG
Cuts at: 0 2233 2370
Size: 2233 137
PciI A'CATG_T
Cuts at: 0 332 2370
Size: 332 2038
PflMI CCAn_nnn'nTGG
Cuts at: 0 2226 2370
Size: 2226 144
PfoI T'CCnGG_A
Cuts at: 0 1574 2370
Size: 1574 796
PspOMI G'GGCC_C
Cuts at: 0 1275 2229 2237 2288 2370
Size: 1275 954 8 51 82
Fragments arranged by size:
1275 954 82 51 8
PstI C_TGCA'G
Cuts at: 0 1609 2370
Size: 1609 761
SacI G_AGCT'C
Cuts at: 0 2195 2370
Size: 2195 175
SapI GCTCTTCn'nnn_
Cuts at: 0 2072 2370
Size: 2072 298
ScaI AGT'ACT
Cuts at: 0 916 1611 2370
Size: 916 695 759
Fragments arranged by size:
916 759 695
SfcI C'TryA_G
Cuts at: 0 729 1605 1628 2370
Size: 729 876 23 742
Fragments arranged by size:
876 742 729 23
SmaI CCC'GGG
Cuts at: 0 1554 2152 2370
Size: 1554 598 218
Fragments arranged by size:
1554 598 218
SmlI C'TyrA_G
Cuts at: 0 388 1775 2133 2370
Size: 388 1387 358 237
Fragments arranged by size:
1387 388 358 237
SphI G_CATG'C
Cuts at: 0 319 2370
Size: 319 2051
SrfI GCCC'GGGC
Cuts at: 0 2152 2370
Size: 2152 218
StuI AGG'CCT
Cuts at: 0 1639 2370
Size: 1639 731
StyI C'CwwG_G
Cuts at: 0 539 776 942 954 1115 1334 1992 2370
Size: 539 237 166 12 161 219 658 378
Fragments arranged by size:
658 539 378 237 219 166 161 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 552 676 1213 1407 2370
Size: 552 124 537 194 963
Fragments arranged by size:
963 552 537 194 124
TatI w'GTAC_w
Cuts at: 0 329 560 914 1055 1609 1889 2370
Size: 329 231 354 141 554 280 481
Fragments arranged by size:
554 481 354 329 280 231 141
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1669 1701 2370
Size: 1669 32 669
Fragments arranged by size:
1669 669 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1968 2223 2370
Size: 1968 255 147
Fragments arranged by size:
1968 255 147
XmaI C'CCGG_G
Cuts at: 0 1552 2150 2370
Size: 1552 598 220
Fragments arranged by size:
1552 598 220
XmnI GAAnn'nnTTC
Cuts at: 0 1043 2029 2345 2370
Size: 1043 986 316 25
Fragments arranged by size:
1043 986 316 25
Enzymes that do cut:
AclI AcuI AflIII AleI AlfI AlwNI ApaI ApoI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BfrBI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiHKAI
BsmI Bsp1286I BspEI BspHI BsrDI BsrFI BstAPI BstEII
BstXI BstYI Bsu36I BtgI BtsI DraI DraIII EarI
EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I HincII HindIII
HpaI MfeI MmeI MslI MspA1I NaeI NcoI NgoMIV
NsiI NspI PasI PciI PflMI PfoI PspOMI PstI
SacI SapI ScaI SfcI SmaI SmlI SphI SrfI
StuI StyI TaqII TatI TstI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AfeI AflII AgeI AhdI
AloI ApaLI AscI AseI AsiSI AvrII BaeI BbeI
BglI BglII BlpI BmgBI BmtI BsaAI BsaXI BsiEI
BsiWI BsmBI BspMI BsrBI BsrGI BssHII BssSI BstBI
BstZ17I BtgZI ClaI CspCI DrdI EaeI EagI EciI
EcoRI EcoRV FalI FseI FspI FspAI KasI KpnI
MluI MscI NarI NdeI NheI NotI NruI PacI
PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI
PvuI PvuII RsrII SacII SalI SanDI SbfI SexAI
SfiI SfoI SgfI SgrAI SnaBI SpeI SspI SwaI
Tth111I XbaI XhoI ZraI