Restriction map for
pF1KB3206
(Linear) (Six-base) MAPSORT of: KIBB3206.seq Check: 4491 from: 1 to: 2385
KIBB3206.orf
With 182 enzymes: SgfI *
July 18, 2007 13:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1499 2385
Size: 1499 886
AatII G_ACGT'C
Cuts at: 0 1331 2385
Size: 1331 1054
AccI GT'mk_AC
Cuts at: 0 2063 2385
Size: 2063 322
AlwNI CAG_nnn'CTG
Cuts at: 0 1087 2385
Size: 1087 1298
ApaI G_GGCC'C
Cuts at: 0 2191 2385
Size: 2191 194
ApoI r'AATT_y
Cuts at: 0 138 1001 2293 2385
Size: 138 863 1292 92
Fragments arranged by size:
1292 863 138 92
AseI AT'TA_AT
Cuts at: 0 704 881 2385
Size: 704 177 1504
Fragments arranged by size:
1504 704 177
AvaI C'yCGr_G
Cuts at: 0 160 191 1437 1785 1834 2385
Size: 160 31 1246 348 49 551
Fragments arranged by size:
1246 551 348 160 49 31
BanI G'GyrC_C
Cuts at: 0 1441 1594 1725 2178 2221 2385
Size: 1441 153 131 453 43 164
Fragments arranged by size:
1441 453 164 153 131 43
BanII G_rGCy'C
Cuts at: 0 82 192 284 362 623 1835 2191 2385
Size: 82 110 92 78 261 1212 356 194
Fragments arranged by size:
1212 356 261 194 110 92 82 78
BbeI G_GCGC'C
Cuts at: 0 1598 2385
Size: 1598 787
BbsI GAAGACnn'nnnn_
Cuts at: 0 170 387 2385
Size: 170 217 1998
Fragments arranged by size:
1998 217 170
BciVI GTATCCnnnnn_n'
Cuts at: 0 1100 2385
Size: 1100 1285
BclI T'GATC_A
Cuts at: 0 381 2385
Size: 381 2004
BlpI GC'TnA_GC
Cuts at: 0 1888 2385
Size: 1888 497
Bme1580I G_kGCm'C
Cuts at: 0 1444 1730 2191 2385
Size: 1444 286 461 194
Fragments arranged by size:
1444 461 286 194
BmgBI CAC'GTC
Cuts at: 0 2214 2385
Size: 2214 171
BmrI ACTGGGnnnn_n'
Cuts at: 0 98 251 2385
Size: 98 153 2134
Fragments arranged by size:
2134 153 98
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 960 2385
Size: 960 1425
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 346 775 1064 2259 2288 2385
Size: 346 429 289 1195 29 97
Fragments arranged by size:
1195 429 346 289 97 29
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1804 2385
Size: 1804 581
BsaI GGTCTCn'nnnn_
Cuts at: 0 1212 1326 1515 2385
Size: 1212 114 189 870
Fragments arranged by size:
1212 870 189 114
BsaAI yAC'GTr
Cuts at: 0 1977 2385
Size: 1977 408
BsaHI Gr'CG_yC
Cuts at: 0 1328 1595 1658 2385
Size: 1328 267 63 727
Fragments arranged by size:
1328 727 267 63
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1594 1624 1981 2011 2385
Size: 1594 30 357 30 374
Fragments arranged by size:
1594 374 357 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 292 2160 2385
Size: 292 1868 225
Fragments arranged by size:
1868 292 225
BseYI C'CCAG_C
Cuts at: 0 60 2000 2385
Size: 60 1940 385
Fragments arranged by size:
1940 385 60
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1387 1698 1957 2160 2385
Size: 1387 311 259 203 225
Fragments arranged by size:
1387 311 259 225 203
BsiEI CG_ry'CG
Cuts at: 0 1739 2165 2169 2385
Size: 1739 426 4 216
Fragments arranged by size:
1739 426 216 4
BsiHKAI G_wGCw'C
Cuts at: 0 284 362 623 1304 1982 2117 2335 2385
Size: 284 78 261 681 678 135 218 50
Fragments arranged by size:
681 678 284 261 218 135 78 50
BsmBI CGTCTCn'nnnn_
Cuts at: 0 795 2385
Size: 795 1590
Bsp1286I G_dGCh'C
Cuts at: 0 82 192 284 362 623 1304 1444 1730
Size: 82 110 92 78 261 681 140 286
Cuts at: 1730 1835 1982 2117 2191 2335 2385
Size: 105 147 135 74 144 50
Fragments arranged by size:
681 286 261 147 144 140 135 110
105 92 82 78 74 50
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1499 2018 2385
Size: 1499 519 367
Fragments arranged by size:
1499 519 367
BsrBI CCG'CTC
Cuts at: 0 122 2385
Size: 122 2263
BstAPI GCAn_nnn'nTGC
Cuts at: 0 42 2385
Size: 42 2343
BstEII G'GTnAC_C
Cuts at: 0 1755 2385
Size: 1755 630
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1767 2385
Size: 1767 618
Bsu36I CC'TnA_GG
Cuts at: 0 1546 2183 2385
Size: 1546 637 202
Fragments arranged by size:
1546 637 202
BtgI C'CryG_G
Cuts at: 0 1605 1826 2385
Size: 1605 221 559
Fragments arranged by size:
1605 559 221
BtsI GCAGTG_nn'
Cuts at: 0 635 2385
Size: 635 1750
EaeI y'GGCC_r
Cuts at: 0 1530 1736 1904 1958 2385
Size: 1530 206 168 54 427
Fragments arranged by size:
1530 427 206 168 54
EagI C'GGCC_G
Cuts at: 0 1736 2385
Size: 1736 649
EarI CTCTTCn'nnn_
Cuts at: 0 602 2104 2110 2385
Size: 602 1502 6 275
Fragments arranged by size:
1502 602 275 6
EcoICRI GAG'CTC
Cuts at: 0 282 360 621 2385
Size: 282 78 261 1764
Fragments arranged by size:
1764 282 261 78
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 346 775 1064 2259 2288 2385
Size: 346 429 289 1195 29 97
Fragments arranged by size:
1195 429 346 289 97 29
EcoNI CCTnn'n_nnAGG
Cuts at: 0 681 2385
Size: 681 1704
EcoO109I rG'GnC_Cy
Cuts at: 0 653 1280 1482 1566 2187 2188 2228 2385
Size: 653 627 202 84 621 1 40 157
Fragments arranged by size:
653 627 621 202 157 84 40 1
EcoRI G'AATT_C
Cuts at: 0 1001 2385
Size: 1001 1384
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 61 2385
Size: 61 2324
FspI TGC'GCA
Cuts at: 0 1873 2385
Size: 1873 512
FspAI rTGC'GCAy
Cuts at: 0 1873 2385
Size: 1873 512
HaeII r_GCGC'y
Cuts at: 0 598 615 1424 1598 1636 2385
Size: 598 17 809 174 38 749
Fragments arranged by size:
809 749 598 174 38 17
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 637 669 793 825 1105 1137 1420 1452
Size: 637 32 124 32 280 32 283 32
Cuts at: 1452 1594 1626 1768 1800 1981 2013 2385
Size: 142 32 142 32 181 32 372
Fragments arranged by size:
637 372 283 280 181 142 142 124
32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 1458 2385
Size: 1458 927
KasI G'GCGC_C
Cuts at: 0 1594 2385
Size: 1594 791
MfeI C'AATT_G
Cuts at: 0 932 2385
Size: 932 1453
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1349 2385
Size: 1349 1036
MslI CAynn'nnrTG
Cuts at: 0 1312 2385
Size: 1312 1073
MspA1I CmG'CkG
Cuts at: 0 228 1224 1692 1815 2385
Size: 228 996 468 123 570
Fragments arranged by size:
996 570 468 228 123
NarI GG'CG_CC
Cuts at: 0 1595 2385
Size: 1595 790
PasI CC'CwG_GG
Cuts at: 0 153 1730 2385
Size: 153 1577 655
Fragments arranged by size:
1577 655 153
PfoI T'CCnGG_A
Cuts at: 0 1154 2018 2196 2385
Size: 1154 864 178 189
Fragments arranged by size:
1154 864 189 178
PmlI CAC'GTG
Cuts at: 0 1977 2385
Size: 1977 408
PpuMI rG'GwC_Cy
Cuts at: 0 653 1280 1482 2228 2385
Size: 653 627 202 746 157
Fragments arranged by size:
746 653 627 202 157
PspOMI G'GGCC_C
Cuts at: 0 2187 2385
Size: 2187 198
PspXI vC'TCGA_Gb
Cuts at: 0 160 2385
Size: 160 2225
PstI C_TGCA'G
Cuts at: 0 29 1574 1697 2385
Size: 29 1545 123 688
Fragments arranged by size:
1545 688 123 29
PvuII CAG'CTG
Cuts at: 0 228 1815 2385
Size: 228 1587 570
Fragments arranged by size:
1587 570 228
SacI G_AGCT'C
Cuts at: 0 284 362 623 2385
Size: 284 78 261 1762
Fragments arranged by size:
1762 284 261 78
SanDI GG'GwC_CC
Cuts at: 0 653 1280 2228 2385
Size: 653 627 948 157
Fragments arranged by size:
948 653 627 157
SapI GCTCTTCn'nnn_
Cuts at: 0 602 2104 2385
Size: 602 1502 281
Fragments arranged by size:
1502 602 281
SbfI CC_TGCA'GG
Cuts at: 0 1574 2385
Size: 1574 811
SexAI A'CCwGG_T
Cuts at: 0 1751 2066 2385
Size: 1751 315 319
Fragments arranged by size:
1751 319 315
SfcI C'TryA_G
Cuts at: 0 25 992 1570 1693 2385
Size: 25 967 578 123 692
Fragments arranged by size:
967 692 578 123 25
SfoI GGC'GCC
Cuts at: 0 1596 2385
Size: 1596 789
SmaI CCC'GGG
Cuts at: 0 1836 2385
Size: 1836 549
SmlI C'TyrA_G
Cuts at: 0 160 1819 2385
Size: 160 1659 566
Fragments arranged by size:
1659 566 160
SrfI GCCC'GGGC
Cuts at: 0 1836 2385
Size: 1836 549
StyI C'CwwG_G
Cuts at: 0 677 768 1760 2385
Size: 677 91 992 625
Fragments arranged by size:
992 677 625 91
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1369 2155 2385
Size: 1369 786 230
Fragments arranged by size:
1369 786 230
TatI w'GTAC_w
Cuts at: 0 1247 2385
Size: 1247 1138
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 149 181 501 533 2192 2224 2385
Size: 149 32 320 32 1659 32 161
Fragments arranged by size:
1659 320 161 149 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 2232 2385
Size: 2232 153
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1914 2385
Size: 1914 471
XhoI C'TCGA_G
Cuts at: 0 160 2385
Size: 160 2225
XmaI C'CCGG_G
Cuts at: 0 1834 2385
Size: 1834 551
XmnI GAAnn'nnTTC
Cuts at: 0 860 2385
Size: 860 1525
ZraI GAC'GTC
Cuts at: 0 1329 2385
Size: 1329 1056
Enzymes that do cut:
AarI AatII AccI AlwNI ApaI ApoI AseI AvaI
BanI BanII BbeI BbsI BciVI BclI BlpI Bme1580I
BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaHI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspMI BsrBI BstAPI BstEII BstXI Bsu36I BtgI BtsI
EaeI EagI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI FspAI HaeII Hin4I HincII KasI MfeI
MmeI MslI MspA1I NarI PasI PfoI PmlI PpuMI
PspOMI PspXI PstI PvuII SacI SanDI SapI SbfI
SexAI SfcI SfoI SmaI SmlI SrfI StyI TaqII
TatI TstI Tth111I XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaLI AscI AsiSI AvrII BaeI
BamHI BbvCI BcgI BfrBI BglI BglII BmtI Bpu10I
BsaBI BsaWI BsiWI BsmI BspEI BspHI BsrDI BsrFI
BsrGI BssHII BssSI BstBI BstYI BstZ17I BtgZI ClaI
CspCI DraI DraIII DrdI EciI EcoRV FseI HindIII
HpaI KpnI MluI MscI NaeI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI PacI PciI PflMI
PmeI PpiI PshAI PsiI PsrI PvuI RsrII SacII
SalI ScaI SfiI SgfI SgrAI SnaBI SpeI SphI
SspI StuI SwaI XbaI