Restriction map for
pF1KB3219
(Linear) (Six-base) MAPSORT of: KIBB3219.seq Check: 9003 from: 1 to: 2007
KIBB3219.orf
With 182 enzymes: SgfI *
February 28, 2007 11:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1508 2007
Size: 1508 499
AccI GT'mk_AC
Cuts at: 0 1565 2007
Size: 1565 442
Acc65I G'GTAC_C
Cuts at: 0 1885 2007
Size: 1885 122
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1321 1570 2007
Size: 1321 249 437
Fragments arranged by size:
1321 437 249
AfeI AGC'GCT
Cuts at: 0 1222 2007
Size: 1222 785
AflIII A'CryG_T
Cuts at: 0 452 2007
Size: 452 1555
AgeI A'CCGG_T
Cuts at: 0 185 2007
Size: 185 1822
AleI CACnn'nnGTG
Cuts at: 0 16 1645 2007
Size: 16 1629 362
Fragments arranged by size:
1629 362 16
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1940 2007
Size: 1940 67
AlwNI CAG_nnn'CTG
Cuts at: 0 63 603 1122 2007
Size: 63 540 519 885
Fragments arranged by size:
885 540 519 63
ApaI G_GGCC'C
Cuts at: 0 954 1434 1815 1938 2007
Size: 954 480 381 123 69
Fragments arranged by size:
954 480 381 123 69
ApaLI G'TGCA_C
Cuts at: 0 220 1527 2007
Size: 220 1307 480
Fragments arranged by size:
1307 480 220
AscI GG'CGCG_CC
Cuts at: 0 248 2007
Size: 248 1759
AvaI C'yCGr_G
Cuts at: 0 1206 1931 2007
Size: 1206 725 76
Fragments arranged by size:
1206 725 76
BanI G'GyrC_C
Cuts at: 0 517 1189 1366 1885 2007
Size: 517 672 177 519 122
Fragments arranged by size:
672 519 517 177 122
BanII G_rGCy'C
Cuts at: 0 810 954 1022 1285 1434 1454 1815 1938
Size: 810 144 68 263 149 20 361 123
Cuts at: 1938 2007
Size: 69
Fragments arranged by size:
810 361 263 149 144 123 69 68
20
BbeI G_GCGC'C
Cuts at: 0 521 2007
Size: 521 1486
BbsI GAAGACnn'nnnn_
Cuts at: 0 773 1849 2007
Size: 773 1076 158
Fragments arranged by size:
1076 773 158
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1356 1390 2007
Size: 1356 34 617
Fragments arranged by size:
1356 617 34
BclI T'GATC_A
Cuts at: 0 1088 2007
Size: 1088 919
BglII A'GATC_T
Cuts at: 0 939 1980 2007
Size: 939 1041 27
Fragments arranged by size:
1041 939 27
BlpI GC'TnA_GC
Cuts at: 0 67 746 2007
Size: 67 679 1261
Fragments arranged by size:
1261 679 67
Bme1580I G_kGCm'C
Cuts at: 0 224 954 1369 1434 1531 1815 1938 2007
Size: 224 730 415 65 97 284 123 69
Fragments arranged by size:
730 415 284 224 123 97 69 65
BmgBI CAC'GTC
Cuts at: 0 165 291 2007
Size: 165 126 1716
Fragments arranged by size:
1716 165 126
BmrI ACTGGGnnnn_n'
Cuts at: 0 1461 2007
Size: 1461 546
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 40 406 448 529 607 984 1099 1291
Size: 40 366 42 81 78 377 115 192
Cuts at: 1291 1534 2005 2007
Size: 243 471 2
Fragments arranged by size:
471 377 366 243 192 115 81 78
42 40 2
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1561 2007
Size: 1561 446
BsaI GGTCTCn'nnnn_
Cuts at: 0 229 2007
Size: 229 1778
BsaAI yAC'GTr
Cuts at: 0 455 920 1332 1686 1908 2007
Size: 455 465 412 354 222 99
Fragments arranged by size:
465 455 412 354 222 99
BsaHI Gr'CG_yC
Cuts at: 0 518 1823 2007
Size: 518 1305 184
Fragments arranged by size:
1305 518 184
BsaWI w'CCGG_w
Cuts at: 0 185 1014 2007
Size: 185 829 993
Fragments arranged by size:
993 829 185
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 813 1087 2007
Size: 813 274 920
Fragments arranged by size:
920 813 274
BseYI C'CCAG_C
Cuts at: 0 606 909 1534 2007
Size: 606 303 625 473
Fragments arranged by size:
625 606 473 303
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1600 1666 1987 2007
Size: 1600 66 321 20
Fragments arranged by size:
1600 321 66 20
BsiEI CG_ry'CG
Cuts at: 0 75 2007
Size: 75 1932
BsiHKAI G_wGCw'C
Cuts at: 0 224 416 1531 1547 2007
Size: 224 192 1115 16 460
Fragments arranged by size:
1115 460 224 192 16
BsmI GAATG_Cn'
Cuts at: 0 1797 2007
Size: 1797 210
Bsp1286I G_dGCh'C
Cuts at: 0 224 416 810 954 1022 1285 1369 1434
Size: 224 192 394 144 68 263 84 65
Cuts at: 1434 1454 1531 1547 1815 1938 2007
Size: 20 77 16 268 123 69
Fragments arranged by size:
394 268 263 224 192 144 123 84
77 69 68 65 20 16
BspHI T'CATG_A
Cuts at: 0 461 1787 2007
Size: 461 1326 220
Fragments arranged by size:
1326 461 220
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 512 717 1013 1508 1517 2007
Size: 512 205 296 495 9 490
Fragments arranged by size:
512 495 490 296 205 9
BsrFI r'CCGG_y
Cuts at: 0 115 185 208 277 440 530 2007
Size: 115 70 23 69 163 90 1477
Fragments arranged by size:
1477 163 115 90 70 69 23
BsrGI T'GTAC_A
Cuts at: 0 20 2007
Size: 20 1987
BssHII G'CGCG_C
Cuts at: 0 248 1583 2007
Size: 248 1335 424
Fragments arranged by size:
1335 424 248
BssSI C'ACGA_G
Cuts at: 0 468 2007
Size: 468 1539
BstAPI GCAn_nnn'nTGC
Cuts at: 0 603 2007
Size: 603 1404
BstXI CCAn_nnnn'nTGG
Cuts at: 0 690 1482 1647 2007
Size: 690 792 165 360
Fragments arranged by size:
792 690 360 165
BstYI r'GATC_y
Cuts at: 0 590 939 1952 1980 2007
Size: 590 349 1013 28 27
Fragments arranged by size:
1013 590 349 28 27
Bsu36I CC'TnA_GG
Cuts at: 0 800 2007
Size: 800 1207
BtgI C'CryG_G
Cuts at: 0 312 890 974 1661 2007
Size: 312 578 84 687 346
Fragments arranged by size:
687 578 346 312 84
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 123 2007
Size: 123 1884
BtsI GCAGTG_nn'
Cuts at: 0 1924 2007
Size: 1924 83
EaeI y'GGCC_r
Cuts at: 0 72 113 153 279 378 434 447 662
Size: 72 41 40 126 99 56 13 215
Cuts at: 662 717 1484 1781 1877 2007
Size: 55 767 297 96 130
Fragments arranged by size:
767 297 215 130 126 99 96 72
56 55 41 40 13
EagI C'GGCC_G
Cuts at: 0 72 2007
Size: 72 1935
EarI CTCTTCn'nnn_
Cuts at: 0 1327 2007
Size: 1327 680
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 40 406 448 529 607 984 1099 1291
Size: 40 366 42 81 78 377 115 192
Cuts at: 1291 1321 1534 1570 2005 2007
Size: 30 213 36 435 2
Fragments arranged by size:
435 377 366 213 192 115 81 78
42 40 36 30 2
EcoNI CCTnn'n_nnAGG
Cuts at: 0 703 2007
Size: 703 1304
EcoO109I rG'GnC_Cy
Cuts at: 0 317 950 951 1119 1431 1811 1935 2007
Size: 317 633 1 168 312 380 124 72
Fragments arranged by size:
633 380 317 312 168 124 72 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1620 2007
Size: 1620 387
FspI TGC'GCA
Cuts at: 0 794 1111 2007
Size: 794 317 896
Fragments arranged by size:
896 794 317
FspAI rTGC'GCAy
Cuts at: 0 1111 2007
Size: 1111 896
HaeII r_GCGC'y
Cuts at: 0 521 542 1087 1224 1483 2007
Size: 521 21 545 137 259 524
Fragments arranged by size:
545 524 521 259 137 21
HincII GTy'rAC
Cuts at: 0 1062 2007
Size: 1062 945
KasI G'GCGC_C
Cuts at: 0 517 2007
Size: 517 1490
KpnI G_GTAC'C
Cuts at: 0 1889 2007
Size: 1889 118
MscI TGG'CCA
Cuts at: 0 436 1486 1879 2007
Size: 436 1050 393 128
Fragments arranged by size:
1050 436 393 128
MslI CAynn'nnrTG
Cuts at: 0 16 227 1624 1645 2007
Size: 16 211 1397 21 362
Fragments arranged by size:
1397 362 211 21 16
MspA1I CmG'CkG
Cuts at: 0 51 71 258 314 600 654 1029 1440
Size: 51 20 187 56 286 54 375 411
Cuts at: 1440 2007
Size: 567
Fragments arranged by size:
567 411 375 286 187 56 54 51
20
NaeI GCC'GGC
Cuts at: 0 117 279 2007
Size: 117 162 1728
Fragments arranged by size:
1728 162 117
NarI GG'CG_CC
Cuts at: 0 518 2007
Size: 518 1489
NcoI C'CATG_G
Cuts at: 0 974 2007
Size: 974 1033
NgoMIV G'CCGG_C
Cuts at: 0 115 277 2007
Size: 115 162 1730
Fragments arranged by size:
1730 162 115
NspI r_CATG'y
Cuts at: 0 1116 1257 2007
Size: 1116 141 750
Fragments arranged by size:
1116 750 141
PasI CC'CwG_GG
Cuts at: 0 1445 1589 2007
Size: 1445 144 418
Fragments arranged by size:
1445 418 144
PfoI T'CCnGG_A
Cuts at: 0 704 1130 1415 1973 2007
Size: 704 426 285 558 34
Fragments arranged by size:
704 558 426 285 34
PmlI CAC'GTG
Cuts at: 0 455 1686 2007
Size: 455 1231 321
Fragments arranged by size:
1231 455 321
PspOMI G'GGCC_C
Cuts at: 0 950 1430 1811 1934 2007
Size: 950 480 381 123 73
Fragments arranged by size:
950 480 381 123 73
PstI C_TGCA'G
Cuts at: 0 50 599 820 1439 1919 2007
Size: 50 549 221 619 480 88
Fragments arranged by size:
619 549 480 221 88 50
PvuII CAG'CTG
Cuts at: 0 51 600 1029 1440 2007
Size: 51 549 429 411 567
Fragments arranged by size:
567 549 429 411 51
SacII CC_GC'GG
Cuts at: 0 315 2007
Size: 315 1692
SexAI A'CCwGG_T
Cuts at: 0 176 401 2007
Size: 176 225 1606
Fragments arranged by size:
1606 225 176
SfcI C'TryA_G
Cuts at: 0 46 595 816 1435 1915 2007
Size: 46 549 221 619 480 92
Fragments arranged by size:
619 549 480 221 92 46
SfoI GGC'GCC
Cuts at: 0 519 2007
Size: 519 1488
SmaI CCC'GGG
Cuts at: 0 1933 2007
Size: 1933 74
SmlI C'TyrA_G
Cuts at: 0 1576 2007
Size: 1576 431
SnaBI TAC'GTA
Cuts at: 0 920 2007
Size: 920 1087
SphI G_CATG'C
Cuts at: 0 1116 2007
Size: 1116 891
SrfI GCCC'GGGC
Cuts at: 0 1933 2007
Size: 1933 74
StyI C'CwwG_G
Cuts at: 0 542 899 974 1475 2007
Size: 542 357 75 501 532
Fragments arranged by size:
542 532 501 357 75
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1135 1384 2007
Size: 1135 249 623
Fragments arranged by size:
1135 623 249
TatI w'GTAC_w
Cuts at: 0 20 2007
Size: 20 1987
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1016 1048 2007
Size: 1016 32 959
Fragments arranged by size:
1016 959 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1074 1359 2007
Size: 1074 285 648
Fragments arranged by size:
1074 648 285
XmaI C'CCGG_G
Cuts at: 0 1931 2007
Size: 1931 76
XmnI GAAnn'nnTTC
Cuts at: 0 1603 2007
Size: 1603 404
Enzymes that do cut:
AarI AccI Acc65I AcuI AfeI AflIII AgeI AleI
AlfI AlwNI ApaI ApaLI AscI AvaI BanI BanII
BbeI BbsI BcgI BclI BglII BlpI Bme1580I BmgBI
BmrI BpmI BpuEI BsaI BsaAI BsaHI BsaWI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI
BsrFI BsrGI BssHII BssSI BstAPI BstXI BstYI Bsu36I
BtgI BtgZI BtsI EaeI EagI EarI Eco57MI EcoNI
EcoO109I FalI FspI FspAI HaeII HincII KasI KpnI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PfoI PmlI PspOMI PstI PvuII SacII SexAI
SfcI SfoI SmaI SmlI SnaBI SphI SrfI StyI
TaqII TatI TstI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AclI AflII AhdI AloI ApoI AseI AsiSI
AvrII BaeI BamHI BbvCI BciVI BfrBI BglI BmtI
BplI Bpu10I BsaBI BsaXI BsiWI BsmBI BspEI BsrBI
BsrDI BstBI BstEII BstZ17I ClaI CspCI DraI DraIII
DrdI EciI EcoICRI EcoRI EcoRV FseI Hin4I HindIII
HpaI MfeI MluI MmeI NdeI NheI NotI NruI
NsiI PacI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PspXI PsrI PvuI RsrII SacI SalI SanDI
SapI SbfI ScaI SfiI SgfI SgrAI SpeI SspI
StuI SwaI Tth111I XbaI XhoI ZraI