Restriction map for
pF1KB3224
(Linear) (Six-base) MAPSORT of: KIBB3224.seq Check: 2782 from: 1 to: 2241
KIBB3224.orf
With 182 enzymes: SgfI *
July 18, 2007 13:36 ..
AccI GT'mk_AC
Cuts at: 0 202 2241
Size: 202 2039
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 433 494 523 701 848 1040 1871 2138
Size: 433 61 29 178 147 192 831 267
Cuts at: 2138 2241
Size: 103
Fragments arranged by size:
831 433 267 192 178 147 103 61
29
AflII C'TTAA_G
Cuts at: 0 1129 2241
Size: 1129 1112
AflIII A'CryG_T
Cuts at: 0 1054 2241
Size: 1054 1187
AhdI GACnn_n'nnGTC
Cuts at: 0 1215 2241
Size: 1215 1026
AleI CACnn'nnGTG
Cuts at: 0 73 2241
Size: 73 2168
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1004 2241
Size: 1004 1237
AlwNI CAG_nnn'CTG
Cuts at: 0 98 331 997 2241
Size: 98 233 666 1244
Fragments arranged by size:
1244 666 233 98
BamHI G'GATC_C
Cuts at: 0 2007 2241
Size: 2007 234
BanII G_rGCy'C
Cuts at: 0 856 2241
Size: 856 1385
BbvCI CC'TCA_GC
Cuts at: 0 1262 2241
Size: 1262 979
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 698 732 2241
Size: 698 34 1509
Fragments arranged by size:
1509 698 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1044 1666 2241
Size: 1044 622 575
Fragments arranged by size:
1044 622 575
BclI T'GATC_A
Cuts at: 0 165 948 2241
Size: 165 783 1293
Fragments arranged by size:
1293 783 165
BfrBI ATG'CAT
Cuts at: 0 838 2241
Size: 838 1403
BglI GCCn_nnn'nGGC
Cuts at: 0 137 2241
Size: 137 2104
BlpI GC'TnA_GC
Cuts at: 0 1517 2241
Size: 1517 724
BmrI ACTGGGnnnn_n'
Cuts at: 0 227 338 444 1756 2241
Size: 227 111 106 1312 485
Fragments arranged by size:
1312 485 227 111 106
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 76 623 808 1523 2017 2241
Size: 76 547 185 715 494 224
Fragments arranged by size:
715 547 494 224 185 76
Bpu10I CC'TnA_GC
Cuts at: 0 1262 2241
Size: 1262 979
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1936 2241
Size: 1936 305
BsaI GGTCTCn'nnnn_
Cuts at: 0 552 1983 2241
Size: 552 1431 258
Fragments arranged by size:
1431 552 258
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 549 579 2241
Size: 549 30 1662
Fragments arranged by size:
1662 549 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 488 570 2241
Size: 488 82 1671
Fragments arranged by size:
1671 488 82
BseYI C'CCAG_C
Cuts at: 0 256 1134 2186 2241
Size: 256 878 1052 55
Fragments arranged by size:
1052 878 256 55
BsmI GAATG_Cn'
Cuts at: 0 721 2241
Size: 721 1520
Bsp1286I G_dGCh'C
Cuts at: 0 856 2241
Size: 856 1385
BspHI T'CATG_A
Cuts at: 0 1308 2241
Size: 1308 933
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1512 2241
Size: 1512 729
BsrDI GCAATG_nn'
Cuts at: 0 856 1328 1938 2241
Size: 856 472 610 303
Fragments arranged by size:
856 610 472 303
BsrFI r'CCGG_y
Cuts at: 0 960 2241
Size: 960 1281
BsrGI T'GTAC_A
Cuts at: 0 457 1564 2241
Size: 457 1107 677
Fragments arranged by size:
1107 677 457
BstEII G'GTnAC_C
Cuts at: 0 75 2241
Size: 75 2166
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1878 2241
Size: 1878 363
BstYI r'GATC_y
Cuts at: 0 756 2007 2241
Size: 756 1251 234
Fragments arranged by size:
1251 756 234
BstZ17I GTA'TAC
Cuts at: 0 203 2241
Size: 203 2038
BtgI C'CryG_G
Cuts at: 0 132 782 2241
Size: 132 650 1459
Fragments arranged by size:
1459 650 132
BtsI GCAGTG_nn'
Cuts at: 0 147 1976 2241
Size: 147 1829 265
Fragments arranged by size:
1829 265 147
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1931 1966 2241
Size: 1931 35 275
Fragments arranged by size:
1931 275 35
DraI TTT'AAA
Cuts at: 0 245 2241
Size: 245 1996
DraIII CAC_nnn'GTG
Cuts at: 0 221 346 2241
Size: 221 125 1895
Fragments arranged by size:
1895 221 125
EaeI y'GGCC_r
Cuts at: 0 732 2241
Size: 732 1509
EarI CTCTTCn'nnn_
Cuts at: 0 971 1676 2241
Size: 971 705 565
Fragments arranged by size:
971 705 565
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 76 433 494 523 623 701 808 848
Size: 76 357 61 29 100 78 107 40
Cuts at: 848 1040 1523 1871 2017 2138 2241
Size: 192 483 348 146 121 103
Fragments arranged by size:
483 357 348 192 146 121 107 103
100 78 76 61 40 29
EcoRV GAT'ATC
Cuts at: 0 899 2241
Size: 899 1342
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1206 1675 2241
Size: 1206 469 566
Fragments arranged by size:
1206 566 469
FspI TGC'GCA
Cuts at: 0 1592 2241
Size: 1592 649
FspAI rTGC'GCAy
Cuts at: 0 1592 2241
Size: 1592 649
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 95 127 549 581 1201 1233 1390 1422
Size: 95 32 422 32 620 32 157 32
Cuts at: 1422 1640 1672 2241
Size: 218 32 569
Fragments arranged by size:
620 569 422 218 157 95 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 1767 2241
Size: 1767 474
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 245 573 1626 1649 2241
Size: 245 328 1053 23 592
Fragments arranged by size:
1053 592 328 245 23
MscI TGG'CCA
Cuts at: 0 734 2241
Size: 734 1507
MslI CAynn'nnrTG
Cuts at: 0 73 2241
Size: 73 2168
MspA1I CmG'CkG
Cuts at: 0 73 98 134 1261 1849 2241
Size: 73 25 36 1127 588 392
Fragments arranged by size:
1127 588 392 73 36 25
NcoI C'CATG_G
Cuts at: 0 782 2241
Size: 782 1459
NdeI CA'TA_TG
Cuts at: 0 1710 2241
Size: 1710 531
NsiI A_TGCA'T
Cuts at: 0 840 2241
Size: 840 1401
NspI r_CATG'y
Cuts at: 0 838 1058 1597 1626 2241
Size: 838 220 539 29 615
Fragments arranged by size:
838 615 539 220 29
PasI CC'CwG_GG
Cuts at: 0 1434 2241
Size: 1434 807
PciI A'CATG_T
Cuts at: 0 1054 2241
Size: 1054 1187
PflMI CCAn_nnn'nTGG
Cuts at: 0 651 1822 2241
Size: 651 1171 419
Fragments arranged by size:
1171 651 419
PfoI T'CCnGG_A
Cuts at: 0 1378 2241
Size: 1378 863
PvuII CAG'CTG
Cuts at: 0 98 1261 1849 2241
Size: 98 1163 588 392
Fragments arranged by size:
1163 588 392 98
SacII CC_GC'GG
Cuts at: 0 135 2241
Size: 135 2106
SapI GCTCTTCn'nnn_
Cuts at: 0 971 2241
Size: 971 1270
ScaI AGT'ACT
Cuts at: 0 271 1291 2241
Size: 271 1020 950
Fragments arranged by size:
1020 950 271
SfcI C'TryA_G
Cuts at: 0 410 1085 2241
Size: 410 675 1156
Fragments arranged by size:
1156 675 410
SmlI C'TyrA_G
Cuts at: 0 1129 1915 2241
Size: 1129 786 326
Fragments arranged by size:
1129 786 326
SphI G_CATG'C
Cuts at: 0 838 2241
Size: 838 1403
SspI AAT'ATT
Cuts at: 0 2164 2241
Size: 2164 77
StyI C'CwwG_G
Cuts at: 0 746 782 1735 2149 2241
Size: 746 36 953 414 92
Fragments arranged by size:
953 746 414 92 36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 209 357 1447 1706 1738 2241
Size: 209 148 1090 259 32 503
Fragments arranged by size:
1090 503 259 209 148 32
TatI w'GTAC_w
Cuts at: 0 158 227 269 457 1289 1564 1685 2241
Size: 158 69 42 188 832 275 121 556
Fragments arranged by size:
832 556 275 188 158 121 69 42
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1566 1598 2241
Size: 1566 32 643
Fragments arranged by size:
1566 643 32
Tth111I GACn'n_nGTC
Cuts at: 0 563 2241
Size: 563 1678
XbaI T'CTAG_A
Cuts at: 0 289 2241
Size: 289 1952
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 919 2241
Size: 919 1322
XmnI GAAnn'nnTTC
Cuts at: 0 719 1367 2241
Size: 719 648 874
Fragments arranged by size:
874 719 648
Enzymes that do cut:
AccI AcuI AflII AflIII AhdI AleI AlfI AlwNI
BamHI BanII BbvCI BcgI BciVI BclI BfrBI BglI
BlpI BmrI BpmI Bpu10I BpuEI BsaI BsaXI BseRI
BseYI BsmI Bsp1286I BspHI BspMI BsrDI BsrFI BsrGI
BstEII BstXI BstYI BstZ17I BtgI BtsI CspCI DraI
DraIII EaeI EarI Eco57MI EcoRV FalI FspI FspAI
Hin4I HincII MmeI MscI MslI MspA1I NcoI NdeI
NsiI NspI PasI PciI PflMI PfoI PvuII SacII
SapI ScaI SfcI SmlI SphI SspI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AgeI AloI ApaI
ApaLI ApoI AscI AseI AsiSI AvaI AvrII BaeI
BanI BbeI BbsI BglII Bme1580I BmgBI BmtI BplI
BsaAI BsaBI BsaHI BsaWI BsgI BsiEI BsiHKAI BsiWI
BsmBI BspEI BsrBI BssHII BssSI BstAPI BstBI Bsu36I
BtgZI ClaI DrdI EagI EciI EcoICRI EcoNI EcoO109I
EcoRI FseI HaeII HindIII HpaI KasI KpnI MfeI
MluI NaeI NarI NgoMIV NheI NotI NruI PacI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI RsrII SacI SalI SanDI SbfI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SrfI StuI SwaI XhoI XmaI ZraI