Restriction map for
pF1KB3226
(Linear) (Six-base) MAPSORT of: KIBB3226.seq Check: 9845 from: 1 to: 1860
KIBB3226.orf
With 182 enzymes: SgfI *
February 28, 2007 11:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 180 1355 1417 1860
Size: 180 1175 62 443
Fragments arranged by size:
1175 443 180 62
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 175 1860
Size: 175 1685
AflIII A'CryG_T
Cuts at: 0 1337 1860
Size: 1337 523
AhdI GACnn_n'nnGTC
Cuts at: 0 228 297 1860
Size: 228 69 1563
Fragments arranged by size:
1563 228 69
AleI CACnn'nnGTG
Cuts at: 0 910 1723 1860
Size: 910 813 137
Fragments arranged by size:
910 813 137
AlwNI CAG_nnn'CTG
Cuts at: 0 107 615 1860
Size: 107 508 1245
Fragments arranged by size:
1245 508 107
ApaI G_GGCC'C
Cuts at: 0 896 1059 1552 1860
Size: 896 163 493 308
Fragments arranged by size:
896 493 308 163
AvaI C'yCGr_G
Cuts at: 0 68 585 817 837 864 889 1123 1174
Size: 68 517 232 20 27 25 234 51
Cuts at: 1174 1567 1860
Size: 393 293
Fragments arranged by size:
517 393 293 234 232 68 51 27
25 20
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1006 1039 1860
Size: 1006 33 821
Fragments arranged by size:
1006 821 33
BamHI G'GATC_C
Cuts at: 0 1759 1860
Size: 1759 101
BanI G'GyrC_C
Cuts at: 0 259 303 494 577 675 763 822 841
Size: 259 44 191 83 98 88 59 19
Cuts at: 841 1860
Size: 1019
Fragments arranged by size:
1019 259 191 98 88 83 59 44
19
BanII G_rGCy'C
Cuts at: 0 547 806 838 896 1059 1310 1552 1823
Size: 547 259 32 58 163 251 242 271
Cuts at: 1823 1860
Size: 37
Fragments arranged by size:
547 271 259 251 242 163 58 37
32
BbeI G_GCGC'C
Cuts at: 0 307 498 581 679 767 826 845 1860
Size: 307 191 83 98 88 59 19 1015
Fragments arranged by size:
1015 307 191 98 88 83 59 19
BbsI GAAGACnn'nnnn_
Cuts at: 0 1438 1677 1860
Size: 1438 239 183
Fragments arranged by size:
1438 239 183
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 226 260 300 334 1860
Size: 226 34 40 34 1526
Fragments arranged by size:
1526 226 40 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1007 1848 1860
Size: 1007 841 12
Fragments arranged by size:
1007 841 12
BglI GCCn_nnn'nGGC
Cuts at: 0 276 655 1322 1331 1860
Size: 276 379 667 9 529
Fragments arranged by size:
667 529 379 276 9
BlpI GC'TnA_GC
Cuts at: 0 1817 1860
Size: 1817 43
Bme1580I G_kGCm'C
Cuts at: 0 212 896 1059 1124 1405 1552 1860
Size: 212 684 163 65 281 147 308
Fragments arranged by size:
684 308 281 212 163 147 65
BmgBI CAC'GTC
Cuts at: 0 1101 1694 1860
Size: 1101 593 166
Fragments arranged by size:
1101 593 166
BmrI ACTGGGnnnn_n'
Cuts at: 0 986 1860
Size: 986 874
BmtI G_CTAG'C
Cuts at: 0 1796 1860
Size: 1796 64
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 707 734 1860
Size: 707 27 1126
Fragments arranged by size:
1126 707 27
Bpu10I CC'TnA_GC
Cuts at: 0 1304 1553 1860
Size: 1304 249 307
Fragments arranged by size:
1304 307 249
BsaHI Gr'CG_yC
Cuts at: 0 197 304 495 578 676 764 823 842
Size: 197 107 191 83 98 88 59 19
Cuts at: 842 1860
Size: 1018
Fragments arranged by size:
1018 197 191 107 98 88 83 59
19
BseYI C'CCAG_C
Cuts at: 0 200 745 896 1860
Size: 200 545 151 964
Fragments arranged by size:
964 545 200 151
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1057 1215 1860
Size: 1057 158 645
Fragments arranged by size:
1057 645 158
BsiEI CG_ry'CG
Cuts at: 0 1031 1860
Size: 1031 829
BsiHKAI G_wGCw'C
Cuts at: 0 101 1310 1559 1823 1860
Size: 101 1209 249 264 37
Fragments arranged by size:
1209 264 249 101 37
Bsp1286I G_dGCh'C
Cuts at: 0 101 212 547 806 838 896 1059 1124
Size: 101 111 335 259 32 58 163 65
Cuts at: 1124 1310 1405 1552 1559 1823 1860
Size: 186 95 147 7 264 37
Fragments arranged by size:
335 264 259 186 163 147 111 101
95 65 58 37 32 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 180 233 1355 1417 1860
Size: 180 53 1122 62 443
Fragments arranged by size:
1122 443 180 62 53
BsrBI CCG'CTC
Cuts at: 0 534 1860
Size: 534 1326
BsrFI r'CCGG_y
Cuts at: 0 491 790 884 981 1860
Size: 491 299 94 97 879
Fragments arranged by size:
879 491 299 97 94
BssHII G'CGCG_C
Cuts at: 0 633 728 856 917 919 921 1860
Size: 633 95 128 61 2 2 939
Fragments arranged by size:
939 633 128 95 61 2 2
BssSI C'ACGA_G
Cuts at: 0 1309 1357 1860
Size: 1309 48 503
Fragments arranged by size:
1309 503 48
BstAPI GCAn_nnn'nTGC
Cuts at: 0 234 615 1238 1860
Size: 234 381 623 622
Fragments arranged by size:
623 622 381 234
BstBI TT'CG_AA
Cuts at: 0 1188 1860
Size: 1188 672
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1242 1860
Size: 1242 618
BstYI r'GATC_y
Cuts at: 0 1736 1759 1860
Size: 1736 23 101
Fragments arranged by size:
1736 101 23
BtgI C'CryG_G
Cuts at: 0 15 176 412 539 572 829 1703 1721
Size: 15 161 236 127 33 257 874 18
Cuts at: 1721 1776 1860
Size: 55 84
Fragments arranged by size:
874 257 236 161 127 84 55 33
18 15
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 413 1860
Size: 413 1447
BtsI GCAGTG_nn'
Cuts at: 0 616 1405 1602 1860
Size: 616 789 197 258
Fragments arranged by size:
789 616 258 197
DraIII CAC_nnn'GTG
Cuts at: 0 131 1860
Size: 131 1729
EaeI y'GGCC_r
Cuts at: 0 7 122 1028 1332 1361 1860
Size: 7 115 906 304 29 499
Fragments arranged by size:
906 499 304 115 29 7
EagI C'GGCC_G
Cuts at: 0 1028 1860
Size: 1028 832
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 866 1860
Size: 866 994
EcoICRI GAG'CTC
Cuts at: 0 1308 1821 1860
Size: 1308 513 39
Fragments arranged by size:
1308 513 39
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 175 707 734 1860
Size: 175 532 27 1126
Fragments arranged by size:
1126 532 175 27
EcoNI CCTnn'n_nnAGG
Cuts at: 0 187 1860
Size: 187 1673
EcoO109I rG'GnC_Cy
Cuts at: 0 718 893 942 1056 1548 1549 1860
Size: 718 175 49 114 492 1 311
Fragments arranged by size:
718 492 311 175 114 49 1
HaeII r_GCGC'y
Cuts at: 0 307 498 581 669 679 752 767 784
Size: 307 191 83 88 10 73 15 17
Cuts at: 784 826 845 1860
Size: 42 19 1015
Fragments arranged by size:
1015 307 191 88 83 73 42 19
17 15 10
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 214 246 283 315 412 444 1155 1187
Size: 214 32 37 32 97 32 711 32
Cuts at: 1187 1744 1751 1776 1783 1860
Size: 557 7 25 7 77
Fragments arranged by size:
711 557 214 97 77 37 32 32
32 32 25 7 7
HincII GTy'rAC
Cuts at: 0 624 1860
Size: 624 1236
KasI G'GCGC_C
Cuts at: 0 303 494 577 675 763 822 841 1860
Size: 303 191 83 98 88 59 19 1019
Fragments arranged by size:
1019 303 191 98 88 83 59 19
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 668 1694 1721 1787 1860
Size: 668 1026 27 66 73
Fragments arranged by size:
1026 668 73 66 27
MscI TGG'CCA
Cuts at: 0 1334 1363 1860
Size: 1334 29 497
Fragments arranged by size:
1334 497 29
MslI CAynn'nnrTG
Cuts at: 0 910 1240 1723 1860
Size: 910 330 483 137
Fragments arranged by size:
910 483 330 137
MspA1I CmG'CkG
Cuts at: 0 107 693 1328 1860
Size: 107 586 635 532
Fragments arranged by size:
635 586 532 107
NaeI GCC'GGC
Cuts at: 0 493 792 1860
Size: 493 299 1068
Fragments arranged by size:
1068 493 299
NarI GG'CG_CC
Cuts at: 0 304 495 578 676 764 823 842 1860
Size: 304 191 83 98 88 59 19 1018
Fragments arranged by size:
1018 304 191 98 88 83 59 19
NgoMIV G'CCGG_C
Cuts at: 0 491 790 1860
Size: 491 299 1070
Fragments arranged by size:
1070 491 299
NheI G'CTAG_C
Cuts at: 0 1792 1860
Size: 1792 68
NspI r_CATG'y
Cuts at: 0 468 1200 1341 1860
Size: 468 732 141 519
Fragments arranged by size:
732 519 468 141
PasI CC'CwG_GG
Cuts at: 0 1830 1860
Size: 1830 30
PciI A'CATG_T
Cuts at: 0 1337 1860
Size: 1337 523
PpuMI rG'GwC_Cy
Cuts at: 0 942 1860
Size: 942 918
PspOMI G'GGCC_C
Cuts at: 0 892 1055 1548 1860
Size: 892 163 493 312
Fragments arranged by size:
892 493 312 163
PstI C_TGCA'G
Cuts at: 0 230 1400 1851 1860
Size: 230 1170 451 9
Fragments arranged by size:
1170 451 230 9
SacI G_AGCT'C
Cuts at: 0 1310 1823 1860
Size: 1310 513 37
Fragments arranged by size:
1310 513 37
SanDI GG'GwC_CC
Cuts at: 0 942 1860
Size: 942 918
SfcI C'TryA_G
Cuts at: 0 226 1396 1847 1860
Size: 226 1170 451 13
Fragments arranged by size:
1170 451 226 13
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1322 1331 1860
Size: 1322 9 529
Fragments arranged by size:
1322 529 9
SfoI GGC'GCC
Cuts at: 0 305 496 579 677 765 824 843 1860
Size: 305 191 83 98 88 59 19 1017
Fragments arranged by size:
1017 305 191 98 88 83 59 19
SgrAI Cr'CCGG_yG
Cuts at: 0 491 1860
Size: 491 1369
SmaI CCC'GGG
Cuts at: 0 70 819 839 866 891 1125 1569 1860
Size: 70 749 20 27 25 234 444 291
Fragments arranged by size:
749 444 291 234 70 27 25 20
SrfI GCCC'GGGC
Cuts at: 0 839 891 1860
Size: 839 52 969
Fragments arranged by size:
969 839 52
StyI C'CwwG_G
Cuts at: 0 1853 1860
Size: 1853 7
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1765 1860
Size: 1765 95
TatI w'GTAC_w
Cuts at: 0 1367 1860
Size: 1367 493
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 651 1860
Size: 651 1209
XmaI C'CCGG_G
Cuts at: 0 68 817 837 864 889 1123 1567 1860
Size: 68 749 20 27 25 234 444 293
Fragments arranged by size:
749 444 293 234 68 27 25 20
XmnI GAAnn'nnTTC
Cuts at: 0 1576 1860
Size: 1576 284
Enzymes that do cut:
AarI AcuI AflIII AhdI AleI AlwNI ApaI AvaI
BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI
BglI BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I
BsaHI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrBI
BsrFI BssHII BssSI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI DraIII EaeI EagI EciI EcoICRI Eco57MI
EcoNI EcoO109I HaeII Hin4I HincII KasI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NheI NspI PasI
PciI PpuMI PspOMI PstI SacI SanDI SfcI SfiI
SfoI SgrAI SmaI SrfI StyI TaqII TatI XcmI
XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AlfI
AloI ApaLI ApoI AscI AseI AsiSI AvrII BbvCI
BclI BfrBI BglII BplI BpuEI BsaI BsaAI BsaBI
BsaWI BsaXI BseRI BsiWI BsmI BsmBI BspEI BspHI
BsrDI BsrGI BstEII BstZ17I Bsu36I ClaI CspCI DraI
DrdI EarI EcoRI EcoRV FalI FseI FspI FspAI
HindIII HpaI KpnI MfeI MluI NcoI NdeI NotI
NruI NsiI PacI PflMI PfoI PmeI PmlI PpiI
PshAI PsiI PspXI PsrI PvuI PvuII RsrII SacII
SalI SapI SbfI ScaI SexAI SgfI SmlI SnaBI
SpeI SphI SspI StuI SwaI TstI Tth111I XbaI
XhoI ZraI