Restriction map for
pF1KB3244
(Linear) (Six-base) MAPSORT of: KIBB3244.seq Check: 4564 from: 1 to: 6486
KIBB3244.orf
With 182 enzymes: SgfI *
July 18, 2007 13:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 3274 4238 5042 5482 6486
Size: 3274 964 804 440 1004
Fragments arranged by size:
3274 1004 964 804 440
AatII G_ACGT'C
Cuts at: 0 5870 6486
Size: 5870 616
AccI GT'mk_AC
Cuts at: 0 64 356 1769 2636 6486
Size: 64 292 1413 867 3850
Fragments arranged by size:
3850 1413 867 292 64
Acc65I G'GTAC_C
Cuts at: 0 474 606 1646 3056 5211 6486
Size: 474 132 1040 1410 2155 1275
Fragments arranged by size:
2155 1410 1275 1040 474 132
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 975 1013 1288 1801 2855 2948 4253 4495
Size: 975 38 275 513 1054 93 1305 242
Cuts at: 4495 5710 6073 6486
Size: 1215 363 413
Fragments arranged by size:
1305 1215 1054 975 513 413 363 275
242 93 38
AfeI AGC'GCT
Cuts at: 0 3136 4633 6486
Size: 3136 1497 1853
Fragments arranged by size:
3136 1853 1497
AflII C'TTAA_G
Cuts at: 0 654 6486
Size: 654 5832
AflIII A'CryG_T
Cuts at: 0 2762 3462 6486
Size: 2762 700 3024
Fragments arranged by size:
3024 2762 700
AhdI GACnn_n'nnGTC
Cuts at: 0 3024 6486
Size: 3024 3462
AleI CACnn'nnGTG
Cuts at: 0 293 1513 4306 5056 6040 6229 6486
Size: 293 1220 2793 750 984 189 257
Fragments arranged by size:
2793 1220 984 750 293 257 189
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1969 2071 3437 6486
Size: 1969 102 1366 3049
Fragments arranged by size:
3049 1969 1366 102
AlwNI CAG_nnn'CTG
Cuts at: 0 290 1335 1633 2490 4282 4374 4384 5208
Size: 290 1045 298 857 1792 92 10 824
Cuts at: 5208 5284 5520 5979 6486
Size: 76 236 459 507
Fragments arranged by size:
1792 1045 857 824 507 459 298 290
236 92 76 10
ApaLI G'TGCA_C
Cuts at: 0 970 4104 4225 4639 6486
Size: 970 3134 121 414 1847
Fragments arranged by size:
3134 1847 970 414 121
ApoI r'AATT_y
Cuts at: 0 1231 1605 2659 6193 6486
Size: 1231 374 1054 3534 293
Fragments arranged by size:
3534 1231 1054 374 293
AvaI C'yCGr_G
Cuts at: 0 109 234 362 1242 1590 2824 3022 5217
Size: 109 125 128 880 348 1234 198 2195
Cuts at: 5217 6329 6486
Size: 1112 157
Fragments arranged by size:
2195 1234 1112 880 348 198 157 128
125 109
AvrII C'CTAG_G
Cuts at: 0 4128 6486
Size: 4128 2358
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 590 623 3048 3081 6486
Size: 590 33 2425 33 3405
Fragments arranged by size:
3405 2425 590 33 33
BamHI G'GATC_C
Cuts at: 0 530 6402 6486
Size: 530 5872 84
Fragments arranged by size:
5872 530 84
BanI G'GyrC_C
Cuts at: 0 474 520 606 1646 2578 2917 3056 3597
Size: 474 46 86 1040 932 339 139 541
Cuts at: 3597 4387 4447 5152 5211 5470 5882 6123 6178
Size: 790 60 705 59 259 412 241 55
Cuts at: 6178 6486
Size: 308
Fragments arranged by size:
1040 932 790 705 541 474 412 339
308 259 241 139 86 60 59 55
46
BanII G_rGCy'C
Cuts at: 0 853 927 1201 1249 1693 1721 1964 2297
Size: 853 74 274 48 444 28 243 333
Cuts at: 2297 2825 3014 6486
Size: 528 189 3472
Fragments arranged by size:
3472 853 528 444 333 274 243 189
74 48 28
BbeI G_GCGC'C
Cuts at: 0 4391 6486
Size: 4391 2095
BbvCI CC'TCA_GC
Cuts at: 0 593 5264 5277 5405 6486
Size: 593 4671 13 128 1081
Fragments arranged by size:
4671 1081 593 128 13
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 560 594 4840 4874 6486
Size: 560 34 4246 34 1612
Fragments arranged by size:
4246 1612 560 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 708 6486
Size: 708 5778
BclI T'GATC_A
Cuts at: 0 1295 2181 5936 6486
Size: 1295 886 3755 550
Fragments arranged by size:
3755 1295 886 550
BglI GCCn_nnn'nGGC
Cuts at: 0 787 1564 1897 6166 6486
Size: 787 777 333 4269 320
Fragments arranged by size:
4269 787 777 333 320
BglII A'GATC_T
Cuts at: 0 143 2789 6486
Size: 143 2646 3697
Fragments arranged by size:
3697 2646 143
BlpI GC'TnA_GC
Cuts at: 0 3088 3721 4619 4978 5785 6486
Size: 3088 633 898 359 807 701
Fragments arranged by size:
3088 898 807 701 633 359
Bme1580I G_kGCm'C
Cuts at: 0 525 974 1510 2581 2920 3934 4085 4108
Size: 525 449 536 1071 339 1014 151 23
Cuts at: 4108 4229 4450 4643 5155 6486
Size: 121 221 193 512 1331
Fragments arranged by size:
1331 1071 1014 536 525 512 449 339
221 193 151 121 23
BmgBI CAC'GTC
Cuts at: 0 3219 3636 6486
Size: 3219 417 2850
Fragments arranged by size:
3219 2850 417
BmrI ACTGGGnnnn_n'
Cuts at: 0 683 930 2500 3508 3674 6090 6486
Size: 683 247 1570 1008 166 2416 396
Fragments arranged by size:
2416 1570 1008 683 396 247 166
BmtI G_CTAG'C
Cuts at: 0 1939 3964 6486
Size: 1939 2025 2522
Fragments arranged by size:
2522 2025 1939
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 921 1737 4515 4821 5442 6486
Size: 921 816 2778 306 621 1044
Fragments arranged by size:
2778 1044 921 816 621 306
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 312 1247 3151 3367 3599 3835 3973 4780
Size: 312 935 1904 216 232 236 138 807
Cuts at: 4780 5092 5915 6486
Size: 312 823 571
Fragments arranged by size:
1904 935 823 807 571 312 312 236
232 216 138
Bpu10I CC'TnA_GC
Cuts at: 0 593 777 1200 1958 1963 2044 3142 3206
Size: 593 184 423 758 5 81 1098 64
Cuts at: 3206 4744 4796 5264 5277 5405 6486
Size: 1538 52 468 13 128 1081
Fragments arranged by size:
1538 1098 1081 758 593 468 423 184
128 81 64 52 13 5
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 538 3694 6486
Size: 538 3156 2792
Fragments arranged by size:
3156 2792 538
BsaI GGTCTCn'nnnn_
Cuts at: 0 217 401 489 652 1513 3119 4486 4927
Size: 217 184 88 163 861 1606 1367 441
Cuts at: 4927 6486
Size: 1559
Fragments arranged by size:
1606 1559 1367 861 441 217 184 163
88
BsaAI yAC'GTr
Cuts at: 0 246 1393 1841 3463 4653 6120 6486
Size: 246 1147 448 1622 1190 1467 366
Fragments arranged by size:
1622 1467 1190 1147 448 366 246
BsaBI GATnn'nnATC
Cuts at: 0 2812 6486
Size: 2812 3674
BsaHI Gr'CG_yC
Cuts at: 0 1820 2633 3878 4020 4346 4388 5867 6486
Size: 1820 813 1245 142 326 42 1479 619
Fragments arranged by size:
1820 1479 1245 813 619 326 142 42
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 10 40 664 694 1568 1598 1786 1816
Size: 10 30 624 30 874 30 188 30
Cuts at: 1816 3581 3611 4879 4909 5065 5095 5765 5795
Size: 1765 30 1268 30 156 30 670 30
Cuts at: 5795 6486
Size: 691
Fragments arranged by size:
1765 1268 874 691 670 624 188 156
30 30 30 30 30 30 30 30
10
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 917 1729 2143 3925 4681 4903 5422 6019
Size: 917 812 414 1782 756 222 519 597
Cuts at: 6019 6223 6486
Size: 204 263
Fragments arranged by size:
1782 917 812 756 597 519 414 263
222 204
BseYI C'CCAG_C
Cuts at: 0 283 402 1084 1641 1737 2002 2351 2913
Size: 283 119 682 557 96 265 349 562
Cuts at: 2913 4009 4291 5092 5769 5888 5944 6009 6130
Size: 1096 282 801 677 119 56 65 121
Cuts at: 6130 6474 6486
Size: 344 12
Fragments arranged by size:
1096 801 682 677 562 557 349 344
283 282 265 121 119 119 96 65
56 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1339 1685 2305 5524 5548 5683 5863 6486
Size: 1339 346 620 3219 24 135 180 623
Fragments arranged by size:
3219 1339 623 620 346 180 135 24
BsiEI CG_ry'CG
Cuts at: 0 2310 6486
Size: 2310 4176
BsiHKAI G_wGCw'C
Cuts at: 0 155 974 1016 1721 1964 2297 2458 2825
Size: 155 819 42 705 243 333 161 367
Cuts at: 2825 3014 4108 4229 4643 5731 5786 5879 5966
Size: 189 1094 121 414 1088 55 93 87
Cuts at: 5966 6236 6486
Size: 270 250
Fragments arranged by size:
1094 1088 819 705 414 367 333 270
250 243 189 161 155 121 93 87
55 42
BsiWI C'GTAC_G
Cuts at: 0 3459 6486
Size: 3459 3027
BsmI GAATG_Cn'
Cuts at: 0 1866 3285 3291 3457 6486
Size: 1866 1419 6 166 3029
Fragments arranged by size:
3029 1866 1419 166 6
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1811 3165 4027 6486
Size: 1811 1354 862 2459
Fragments arranged by size:
2459 1811 1354 862
Bsp1286I G_dGCh'C
Cuts at: 0 155 525 853 927 974 1016 1201 1249
Size: 155 370 328 74 47 42 185 48
Cuts at: 1249 1510 1693 1721 1964 2297 2458 2581 2825
Size: 261 183 28 243 333 161 123 244
Cuts at: 2825 2920 3014 3934 4085 4108 4229 4450 4643
Size: 95 94 920 151 23 121 221 193
Cuts at: 4643 5155 5731 5786 5879 5966 6236 6486
Size: 512 576 55 93 87 270 250
Fragments arranged by size:
920 576 512 370 333 328 270 261
250 244 243 221 193 185 183 161
155 151 123 121 95 94 93 87
74 55 48 47 42 28 23
BspHI T'CATG_A
Cuts at: 0 6323 6486
Size: 6323 163
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2402 3274 4238 4723 5000 5042 5193 5482
Size: 2402 872 964 485 277 42 151 289
Cuts at: 5482 6486
Size: 1004
Fragments arranged by size:
2402 1004 964 872 485 289 277 151
42
BsrBI CCG'CTC
Cuts at: 0 1116 3092 3351 5673 6486
Size: 1116 1976 259 2322 813
Fragments arranged by size:
2322 1976 1116 813 259
BsrDI GCAATG_nn'
Cuts at: 0 3419 6486
Size: 3419 3067
BsrFI r'CCGG_y
Cuts at: 0 91 3676 4016 4973 6486
Size: 91 3585 340 957 1513
Fragments arranged by size:
3585 1513 957 340 91
BsrGI T'GTAC_A
Cuts at: 0 6443 6486
Size: 6443 43
BssSI C'ACGA_G
Cuts at: 0 1729 6486
Size: 1729 4757
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1672 5004 6363 6486
Size: 1672 3332 1359 123
Fragments arranged by size:
3332 1672 1359 123
BstBI TT'CG_AA
Cuts at: 0 2601 6486
Size: 2601 3885
BstEII G'GTnAC_C
Cuts at: 0 670 3312 6046 6486
Size: 670 2642 2734 440
Fragments arranged by size:
2734 2642 670 440
BstXI CCAn_nnnn'nTGG
Cuts at: 0 49 883 920 2877 3345 3498 6474 6486
Size: 49 834 37 1957 468 153 2976 12
Fragments arranged by size:
2976 1957 834 468 153 49 37 12
BstYI r'GATC_y
Cuts at: 0 143 530 566 749 2495 2789 3737 4670
Size: 143 387 36 183 1746 294 948 933
Cuts at: 4670 5067 6402 6486
Size: 397 1335 84
Fragments arranged by size:
1746 1335 948 933 397 387 294 183
143 84 36
BtgI C'CryG_G
Cuts at: 0 499 882 2370 2852 2882 3050 3176 3227
Size: 499 383 1488 482 30 168 126 51
Cuts at: 3227 4304 4451 4595 5325 5759 6174 6486
Size: 1077 147 144 730 434 415 312
Fragments arranged by size:
1488 1077 730 499 482 434 415 383
312 168 147 144 126 51 30
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2325 3240 6486
Size: 2325 915 3246
Fragments arranged by size:
3246 2325 915
BtsI GCAGTG_nn'
Cuts at: 0 907 2513 2908 3332 5647 5906 5919 6486
Size: 907 1606 395 424 2315 259 13 567
Fragments arranged by size:
2315 1606 907 567 424 395 259 13
ClaI AT'CG_AT
Cuts at: 0 4094 5195 6486
Size: 4094 1101 1291
Fragments arranged by size:
4094 1291 1101
DraIII CAC_nnn'GTG
Cuts at: 0 2190 3502 6486
Size: 2190 1312 2984
Fragments arranged by size:
2984 2190 1312
DrdI GACnn_nn'nnGTC
Cuts at: 0 28 4522 6486
Size: 28 4494 1964
Fragments arranged by size:
4494 1964 28
EaeI y'GGCC_r
Cuts at: 0 1218 1337 1889 2539 2879 3521 3674 4064
Size: 1218 119 552 650 340 642 153 390
Cuts at: 4064 4454 5245 5349 5909 6486
Size: 390 791 104 560 577
Fragments arranged by size:
1218 791 650 642 577 560 552 390
390 340 153 119 104
EarI CTCTTCn'nnn_
Cuts at: 0 655 937 2083 4032 6486
Size: 655 282 1146 1949 2454
Fragments arranged by size:
2454 1949 1146 655 282
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 5149 6412 6486
Size: 5149 1263 74
Fragments arranged by size:
5149 1263 74
EcoICRI GAG'CTC
Cuts at: 0 1719 1962 2295 2823 3012 6486
Size: 1719 243 333 528 189 3474
Fragments arranged by size:
3474 1719 528 333 243 189
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 312 975 1013 1247 1288 1801 2855 2948
Size: 312 663 38 234 41 513 1054 93
Cuts at: 2948 3151 3367 3599 3835 3973 4253 4495 4780
Size: 203 216 232 236 138 280 242 285
Cuts at: 4780 5092 5710 5915 6073 6486
Size: 312 618 205 158 413
Fragments arranged by size:
1054 663 618 513 413 312 312 285
280 242 236 234 232 216 205 203
158 138 93 41 38
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1270 1707 5132 6486
Size: 1270 437 3425 1354
Fragments arranged by size:
3425 1354 1270 437
EcoO109I rG'GnC_Cy
Cuts at: 0 114 1206 1263 1500 2040 2145 3400 4125
Size: 114 1092 57 237 540 105 1255 725
Cuts at: 4125 4418 4771 4907 5525 5976 6486
Size: 293 353 136 618 451 510
Fragments arranged by size:
1255 1092 725 618 540 510 451 353
293 237 136 114 105 57
EcoRI G'AATT_C
Cuts at: 0 6193 6486
Size: 6193 293
EcoRV GAT'ATC
Cuts at: 0 1053 3330 4098 6486
Size: 1053 2277 768 2388
Fragments arranged by size:
2388 2277 1053 768
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 4278 6486
Size: 4278 2208
FspI TGC'GCA
Cuts at: 0 2152 6139 6486
Size: 2152 3987 347
Fragments arranged by size:
3987 2152 347
HaeII r_GCGC'y
Cuts at: 0 861 1131 3138 4391 4635 4767 6217 6486
Size: 861 270 2007 1253 244 132 1450 269
Fragments arranged by size:
2007 1450 1253 861 270 269 244 132
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 10 42 664 696 1137 1169 1524 1556
Size: 10 32 622 32 441 32 355 32
Cuts at: 1556 2776 2808 3010 3042 5605 5637 6486
Size: 1220 32 202 32 2563 32 849
Fragments arranged by size:
2563 1220 849 622 441 355 202 32
32 32 32 32 32 32 10
HincII GTy'rAC
Cuts at: 0 65 4170 6375 6486
Size: 65 4105 2205 111
Fragments arranged by size:
4105 2205 111 65
HindIII A'AGCT_T
Cuts at: 0 4189 4477 6287 6486
Size: 4189 288 1810 199
Fragments arranged by size:
4189 1810 288 199
KasI G'GCGC_C
Cuts at: 0 4387 6486
Size: 4387 2099
KpnI G_GTAC'C
Cuts at: 0 478 610 1650 3060 5215 6486
Size: 478 132 1040 1410 2155 1271
Fragments arranged by size:
2155 1410 1271 1040 478 132
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2195 2248 5411 5937 6486
Size: 2195 53 3163 526 549
Fragments arranged by size:
3163 2195 549 526 53
MscI TGG'CCA
Cuts at: 0 1220 1891 2541 2881 3523 5247 6486
Size: 1220 671 650 340 642 1724 1239
Fragments arranged by size:
1724 1239 1220 671 650 642 340
MslI CAynn'nnrTG
Cuts at: 0 293 1513 1855 2722 2881 3296 4306 5056
Size: 293 1220 342 867 159 415 1010 750
Cuts at: 5056 6040 6229 6451 6486
Size: 984 189 222 35
Fragments arranged by size:
1220 1010 984 867 750 415 342 293
222 189 159 35
MspA1I CmG'CkG
Cuts at: 0 1084 1633 2254 2312 2355 2533 2865 3438
Size: 1084 549 621 58 43 178 332 573
Cuts at: 3438 3695 3856 3892 3942 4069 4282 5404 6176
Size: 257 161 36 50 127 213 1122 772
Cuts at: 6176 6486
Size: 310
Fragments arranged by size:
1122 1084 772 621 573 549 332 310
257 213 178 161 127 58 50 43
36
NaeI GCC'GGC
Cuts at: 0 3678 4018 4975 6486
Size: 3678 340 957 1511
Fragments arranged by size:
3678 1511 957 340
NarI GG'CG_CC
Cuts at: 0 4388 6486
Size: 4388 2098
NcoI C'CATG_G
Cuts at: 0 882 2852 2882 4304 5759 6486
Size: 882 1970 30 1422 1455 727
Fragments arranged by size:
1970 1455 1422 882 727 30
NdeI CA'TA_TG
Cuts at: 0 4645 6486
Size: 4645 1841
NgoMIV G'CCGG_C
Cuts at: 0 3676 4016 4973 6486
Size: 3676 340 957 1513
Fragments arranged by size:
3676 1513 957 340
NheI G'CTAG_C
Cuts at: 0 1935 3960 6486
Size: 1935 2025 2526
Fragments arranged by size:
2526 2025 1935
NspI r_CATG'y
Cuts at: 0 1585 2350 2766 3274 3373 4168 5103 6144
Size: 1585 765 416 508 99 795 935 1041
Cuts at: 6144 6444 6486
Size: 300 42
Fragments arranged by size:
1585 1041 935 795 765 508 416 300
99 42
PasI CC'CwG_GG
Cuts at: 0 611 1093 1141 1329 1677 4924 5335 6486
Size: 611 482 48 188 348 3247 411 1151
Fragments arranged by size:
3247 1151 611 482 411 348 188 48
PciI A'CATG_T
Cuts at: 0 2762 6486
Size: 2762 3724
PflMI CCAn_nnn'nTGG
Cuts at: 0 290 771 1335 3051 4374 4533 4818 4884
Size: 290 481 564 1716 1323 159 285 66
Cuts at: 4884 5520 6486
Size: 636 966
Fragments arranged by size:
1716 1323 966 636 564 481 290 285
159 66
PfoI T'CCnGG_A
Cuts at: 0 3197 3950 6486
Size: 3197 753 2536
Fragments arranged by size:
3197 2536 753
PmlI CAC'GTG
Cuts at: 0 4653 6486
Size: 4653 1833
PpuMI rG'GwC_Cy
Cuts at: 0 1500 2040 3400 5525 6486
Size: 1500 540 1360 2125 961
Fragments arranged by size:
2125 1500 1360 961 540
PspXI vC'TCGA_Gb
Cuts at: 0 3022 6486
Size: 3022 3464
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 6427 6459 6486
Size: 6427 32 27
Fragments arranged by size:
6427 32 27
PstI C_TGCA'G
Cuts at: 0 326 3416 4467 5000 6486
Size: 326 3090 1051 533 1486
Fragments arranged by size:
3090 1486 1051 533 326
PvuII CAG'CTG
Cuts at: 0 1084 2254 2355 2533 2865 3438 3856 3892
Size: 1084 1170 101 178 332 573 418 36
Cuts at: 3892 5404 6486
Size: 1512 1082
Fragments arranged by size:
1512 1170 1084 1082 573 418 332 178
101 36
SacI G_AGCT'C
Cuts at: 0 1721 1964 2297 2825 3014 6486
Size: 1721 243 333 528 189 3472
Fragments arranged by size:
3472 1721 528 333 243 189
SacII CC_GC'GG
Cuts at: 0 6177 6486
Size: 6177 309
SalI G'TCGA_C
Cuts at: 0 63 6486
Size: 63 6423
SanDI GG'GwC_CC
Cuts at: 0 5525 6486
Size: 5525 961
SapI GCTCTTCn'nnn_
Cuts at: 0 2083 6486
Size: 2083 4403
ScaI AGT'ACT
Cuts at: 0 3663 6486
Size: 3663 2823
SexAI A'CCwGG_T
Cuts at: 0 4239 6486
Size: 4239 2247
SfcI C'TryA_G
Cuts at: 0 322 2675 3388 3412 3828 4463 4996 6486
Size: 322 2353 713 24 416 635 533 1490
Fragments arranged by size:
2353 1490 713 635 533 416 322 24
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1897 6166 6486
Size: 1897 4269 320
Fragments arranged by size:
4269 1897 320
SfoI GGC'GCC
Cuts at: 0 4389 6486
Size: 4389 2097
SmaI CCC'GGG
Cuts at: 0 5219 6331 6486
Size: 5219 1112 155
Fragments arranged by size:
5219 1112 155
SmlI C'TyrA_G
Cuts at: 0 553 654 2824 3022 3709 6486
Size: 553 101 2170 198 687 2777
Fragments arranged by size:
2777 2170 687 553 198 101
SnaBI TAC'GTA
Cuts at: 0 1393 6486
Size: 1393 5093
SpeI A'CTAG_T
Cuts at: 0 5290 6486
Size: 5290 1196
SphI G_CATG'C
Cuts at: 0 3373 6144 6486
Size: 3373 2771 342
Fragments arranged by size:
3373 2771 342
SspI AAT'ATT
Cuts at: 0 1308 6486
Size: 1308 5178
StuI AGG'CCT
Cuts at: 0 2134 6486
Size: 2134 4352
StyI C'CwwG_G
Cuts at: 0 882 1466 2622 2852 2882 3425 3518 4040
Size: 882 584 1156 230 30 543 93 522
Cuts at: 4040 4128 4304 4413 4442 4883 5111 5609 5759
Size: 88 176 109 29 441 228 498 150
Cuts at: 5759 6486
Size: 727
Fragments arranged by size:
1156 882 727 584 543 522 498 441
230 228 176 150 109 93 88 30
29
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 18 199 1022 1549 1669 2599 2845 3490
Size: 18 181 823 527 120 930 246 645
Cuts at: 3490 3634 5986 6034 6109 6162 6486
Size: 144 2352 48 75 53 324
Fragments arranged by size:
2352 930 823 645 527 324 246 181
144 120 75 53 48 18
TatI w'GTAC_w
Cuts at: 0 3661 6069 6443 6486
Size: 3661 2408 374 43
Fragments arranged by size:
3661 2408 374 43
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1568 1600 1893 1925 2597 2629 3581 3613
Size: 1568 32 293 32 672 32 952 32
Cuts at: 3613 3913 3945 4813 4845 4879 4904 4911 4936
Size: 300 32 868 32 34 25 7 25
Cuts at: 4936 5065 5097 5765 5797 6486
Size: 129 32 668 32 689
Fragments arranged by size:
1568 952 868 689 672 668 300 293
129 34 32 32 32 32 32 32
32 32 25 25 7
Tth111I GACn'n_nGTC
Cuts at: 0 1345 5869 6486
Size: 1345 4524 617
Fragments arranged by size:
4524 1345 617
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1938 4116 5766 6342 6486
Size: 1938 2178 1650 576 144
Fragments arranged by size:
2178 1938 1650 576 144
XhoI C'TCGA_G
Cuts at: 0 2824 3022 6486
Size: 2824 198 3464
Fragments arranged by size:
3464 2824 198
XmaI C'CCGG_G
Cuts at: 0 5217 6329 6486
Size: 5217 1112 157
Fragments arranged by size:
5217 1112 157
XmnI GAAnn'nnTTC
Cuts at: 0 2953 6486
Size: 2953 3533
ZraI GAC'GTC
Cuts at: 0 5868 6486
Size: 5868 618
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AfeI AflII AflIII
AhdI AleI AlfI AlwNI ApaLI ApoI AvaI AvrII
BaeI BamHI BanI BanII BbeI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI
BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI DraIII DrdI EaeI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NspI
PasI PciI PflMI PfoI PmlI PpuMI PspXI PsrI
PstI PvuII SacI SacII SalI SanDI SapI ScaI
SexAI SfcI SfiI SfoI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AclI AgeI AloI ApaI AscI AseI AsiSI BbsI
BfrBI BsaWI BspEI BssHII BstZ17I Bsu36I CspCI DraI
EagI FseI FspAI HpaI MfeI MluI NotI NruI
NsiI PacI PmeI PpiI PshAI PsiI PspOMI PvuI
RsrII SbfI SgfI SgrAI SrfI SwaI XbaI