(Linear) (Six-base) MAPSORT of: KIBB3248.seq  Check: 3058  from: 1  to: 630



KIBB3248.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:23  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     190     630 
   Size:        190     440
 
ApoI r'AATT_y

Cuts at:      0     121     331     343     630 
   Size:        121     210      12     287

  Fragments arranged by size: 

                 287     210     121      12
 
AvaI C'yCGr_G

Cuts at:      0     539     630 
   Size:        539      91
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     474     630 
   Size:        474     156
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      46     630 
   Size:         46     584
 
BspHI T'CATG_A

Cuts at:      0     117     630 
   Size:        117     513
 
BstEII G'GTnAC_C

Cuts at:      0       6     630 
   Size:          6     624
 
BstYI r'GATC_y

Cuts at:      0     159     630 
   Size:        159     471
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      54     630 
   Size:         54     576
 
EaeI y'GGCC_r

Cuts at:      0     336     630 
   Size:        336     294
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     190     474     630 
   Size:        190     284     156

  Fragments arranged by size: 

                 284     190     156
 
EcoO109I rG'GnC_Cy

Cuts at:      0     556     630 
   Size:        556      74
 
EcoRI G'AATT_C

Cuts at:      0     121     630 
   Size:        121     509
 
MscI TGG'CCA

Cuts at:      0     338     630 
   Size:        338     292
 
NspI r_CATG'y

Cuts at:      0      43     312     630 
   Size:         43     269     318

  Fragments arranged by size: 

                 318     269      43
 
PpuMI rG'GwC_Cy

Cuts at:      0     556     630 
   Size:        556      74
 
PspXI vC'TCGA_Gb

Cuts at:      0     539     630 
   Size:        539      91
 
PstI C_TGCA'G

Cuts at:      0      38     201     630 
   Size:         38     163     429

  Fragments arranged by size: 

                 429     163      38
 
SanDI GG'GwC_CC

Cuts at:      0     556     630 
   Size:        556      74
 
SfcI C'TryA_G

Cuts at:      0      34     197     514     630 
   Size:         34     163     317     116

  Fragments arranged by size: 

                 317     163     116      34
 
SmlI C'TyrA_G

Cuts at:      0     539     630 
   Size:        539      91
 
TatI w'GTAC_w

Cuts at:      0     220     630 
   Size:        220     410
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     561     593     630 
   Size:        561      32      37

  Fragments arranged by size: 

                 561      37      32
 
XhoI C'TCGA_G

Cuts at:      0     539     630 
   Size:        539      91
 

 Enzymes that do cut:

     AcuI     ApoI     AvaI     BpmI    BseRI    BspHI   BstEII    BstYI
    BtgZI     EaeI  Eco57MI EcoO109I    EcoRI     MscI     NspI    PpuMI
    PspXI     PstI    SanDI     SfcI     SmlI     TatI     TstI     XhoI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseYI
     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI    BstXI  BstZ17I   Bsu36I     BtgI     BtsI     ClaI    CspCI
     DraI   DraIII     DrdI     EagI     EarI     EciI  EcoICRI    EcoNI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MslI
   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PshAI     PsiI   PspOMI     PsrI     PvuI    PvuII
    RsrII     SacI    SacII     SalI     SapI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     StyI     SwaI    TaqII  Tth111I     XbaI
     XcmI     XmaI     XmnI     ZraI