Restriction map for
pF1KB3264
(Linear) (Six-base) MAPSORT of: KIBB3264.seq Check: 4609 from: 1 to: 2541
KIBB3264.orf
With 182 enzymes: SgfI *
April 24, 2009 13:01 ..
AccI GT'mk_AC
Cuts at: 0 1676 2541
Size: 1676 865
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 190 1324 2541
Size: 190 1134 1217
Fragments arranged by size:
1217 1134 190
AflIII A'CryG_T
Cuts at: 0 987 2541
Size: 987 1554
AleI CACnn'nnGTG
Cuts at: 0 2524 2541
Size: 2524 17
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 64 2541
Size: 64 2477
AlwNI CAG_nnn'CTG
Cuts at: 0 1020 2541
Size: 1020 1521
ApoI r'AATT_y
Cuts at: 0 688 949 1167 1217 1441 1920 2203 2541
Size: 688 261 218 50 224 479 283 338
Fragments arranged by size:
688 479 338 283 261 224 218 50
AseI AT'TA_AT
Cuts at: 0 404 2060 2541
Size: 404 1656 481
Fragments arranged by size:
1656 481 404
AvaI C'yCGr_G
Cuts at: 0 1565 2541
Size: 1565 976
BamHI G'GATC_C
Cuts at: 0 1770 2541
Size: 1770 771
BanI G'GyrC_C
Cuts at: 0 657 2541
Size: 657 1884
BbsI GAAGACnn'nnnn_
Cuts at: 0 419 2541
Size: 419 2122
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2361 2395 2541
Size: 2361 34 146
Fragments arranged by size:
2361 146 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1860 2541
Size: 1860 681
BfrBI ATG'CAT
Cuts at: 0 1554 2541
Size: 1554 987
BglII A'GATC_T
Cuts at: 0 471 1335 2541
Size: 471 864 1206
Fragments arranged by size:
1206 864 471
Bme1580I G_kGCm'C
Cuts at: 0 1985 2541
Size: 1985 556
BmrI ACTGGGnnnn_n'
Cuts at: 0 195 2541
Size: 195 2346
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 142 2541
Size: 142 2399
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 997 1840 1952 2042 2541
Size: 997 843 112 90 499
Fragments arranged by size:
997 843 499 112 90
Bpu10I CC'TnA_GC
Cuts at: 0 83 2541
Size: 83 2458
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2236 2541
Size: 2236 305
BsaI GGTCTCn'nnnn_
Cuts at: 0 1120 2041 2541
Size: 1120 921 500
Fragments arranged by size:
1120 921 500
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2426 2456 2541
Size: 2426 30 85
Fragments arranged by size:
2426 85 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1000 2498 2541
Size: 1000 1498 43
Fragments arranged by size:
1498 1000 43
BseYI C'CCAG_C
Cuts at: 0 1824 2367 2541
Size: 1824 543 174
Fragments arranged by size:
1824 543 174
BsiHKAI G_wGCw'C
Cuts at: 0 1653 2541
Size: 1653 888
BsmBI CGTCTCn'nnnn_
Cuts at: 0 244 2541
Size: 244 2297
Bsp1286I G_dGCh'C
Cuts at: 0 1653 1985 2541
Size: 1653 332 556
Fragments arranged by size:
1653 556 332
BspHI T'CATG_A
Cuts at: 0 756 2541
Size: 756 1785
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1782 2541
Size: 1782 759
BsrDI GCAATG_nn'
Cuts at: 0 36 2033 2186 2372 2541
Size: 36 1997 153 186 169
Fragments arranged by size:
1997 186 169 153 36
BssHII G'CGCG_C
Cuts at: 0 20 2541
Size: 20 2521
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1586 2541
Size: 1586 955
BstBI TT'CG_AA
Cuts at: 0 1221 2541
Size: 1221 1320
BstEII G'GTnAC_C
Cuts at: 0 66 2541
Size: 66 2475
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2526 2541
Size: 2526 15
BstYI r'GATC_y
Cuts at: 0 471 1335 1770 2541
Size: 471 864 435 771
Fragments arranged by size:
864 771 471 435
Bsu36I CC'TnA_GG
Cuts at: 0 184 1202 1600 2541
Size: 184 1018 398 941
Fragments arranged by size:
1018 941 398 184
BtgI C'CryG_G
Cuts at: 0 7 2541
Size: 7 2534
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 128 2364 2541
Size: 128 2236 177
Fragments arranged by size:
2236 177 128
BtsI GCAGTG_nn'
Cuts at: 0 22 31 852 1289 2541
Size: 22 9 821 437 1252
Fragments arranged by size:
1252 821 437 22 9
DraI TTT'AAA
Cuts at: 0 995 1466 2208 2541
Size: 995 471 742 333
Fragments arranged by size:
995 742 471 333
DraIII CAC_nnn'GTG
Cuts at: 0 66 2541
Size: 66 2475
DrdI GACnn_nn'nnGTC
Cuts at: 0 2103 2541
Size: 2103 438
EaeI y'GGCC_r
Cuts at: 0 365 2541
Size: 365 2176
EarI CTCTTCn'nnn_
Cuts at: 0 962 1665 2525 2541
Size: 962 703 860 16
Fragments arranged by size:
962 860 703 16
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 190 997 1324 1840 1952 2042 2541
Size: 190 807 327 516 112 90 499
Fragments arranged by size:
807 516 499 327 190 112 90
EcoO109I rG'GnC_Cy
Cuts at: 0 380 1840 2541
Size: 380 1460 701
Fragments arranged by size:
1460 701 380
FspI TGC'GCA
Cuts at: 0 101 2541
Size: 101 2440
MfeI C'AATT_G
Cuts at: 0 1680 2541
Size: 1680 861
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1066 1920 2541
Size: 1066 854 621
Fragments arranged by size:
1066 854 621
MscI TGG'CCA
Cuts at: 0 367 2541
Size: 367 2174
MslI CAynn'nnrTG
Cuts at: 0 2458 2524 2541
Size: 2458 66 17
Fragments arranged by size:
2458 66 17
MspA1I CmG'CkG
Cuts at: 0 135 2317 2541
Size: 135 2182 224
Fragments arranged by size:
2182 224 135
NdeI CA'TA_TG
Cuts at: 0 2523 2541
Size: 2523 18
NsiI A_TGCA'T
Cuts at: 0 1556 2541
Size: 1556 985
PflMI CCAn_nnn'nTGG
Cuts at: 0 1020 2541
Size: 1020 1521
PfoI T'CCnGG_A
Cuts at: 0 1013 2541
Size: 1013 1528
PpuMI rG'GwC_Cy
Cuts at: 0 1840 2541
Size: 1840 701
PspXI vC'TCGA_Gb
Cuts at: 0 1565 2541
Size: 1565 976
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 2259 2291 2541
Size: 2259 32 250
Fragments arranged by size:
2259 250 32
PstI C_TGCA'G
Cuts at: 0 847 2288 2541
Size: 847 1441 253
Fragments arranged by size:
1441 847 253
PvuII CAG'CTG
Cuts at: 0 135 2317 2541
Size: 135 2182 224
Fragments arranged by size:
2182 224 135
ScaI AGT'ACT
Cuts at: 0 841 2076 2233 2541
Size: 841 1235 157 308
Fragments arranged by size:
1235 841 308 157
SfcI C'TryA_G
Cuts at: 0 356 843 1160 2284 2541
Size: 356 487 317 1124 257
Fragments arranged by size:
1124 487 356 317 257
SmlI C'TyrA_G
Cuts at: 0 1565 2251 2541
Size: 1565 686 290
Fragments arranged by size:
1565 686 290
SspI AAT'ATT
Cuts at: 0 649 2541
Size: 649 1892
StuI AGG'CCT
Cuts at: 0 428 2541
Size: 428 2113
StyI C'CwwG_G
Cuts at: 0 1422 2541
Size: 1422 1119
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2065 2541
Size: 2065 476
TatI w'GTAC_w
Cuts at: 0 839 1346 2074 2132 2231 2541
Size: 839 507 728 58 99 310
Fragments arranged by size:
839 728 507 310 99 58
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2426 2458 2541
Size: 2426 32 83
Fragments arranged by size:
2426 83 32
XbaI T'CTAG_A
Cuts at: 0 1233 2541
Size: 1233 1308
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1086 1429 2541
Size: 1086 343 1112
Fragments arranged by size:
1112 1086 343
XhoI C'TCGA_G
Cuts at: 0 1565 2541
Size: 1565 976
Enzymes that do cut:
AccI AcuI AflIII AleI AlfI AlwNI ApoI AseI
AvaI BamHI BanI BbsI BcgI BciVI BfrBI BglII
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaXI
BseRI BseYI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrDI
BssHII BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EarI Eco57MI
EcoO109I FspI MfeI MmeI MscI MslI MspA1I NdeI
NsiI PflMI PfoI PpuMI PspXI PsrI PstI PvuII
ScaI SfcI SmlI SspI StuI StyI TaqII TatI
TstI XbaI XcmI XhoI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AloI ApaI ApaLI AscI AsiSI AvrII BaeI BanII
BbeI BbvCI BclI BglI BlpI BmgBI BmtI BsaAI
BsaBI BsaHI BsaWI BsgI BsiEI BsiWI BsmI BspEI
BsrBI BsrFI BsrGI BssSI BstZ17I ClaI CspCI EagI
EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspAI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MluI
NaeI NarI NcoI NgoMIV NheI NotI NruI NspI
PacI PasI PciI PmeI PmlI PpiI PshAI PsiI
PspOMI PvuI RsrII SacI SacII SalI SanDI SapI
SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SwaI Tth111I XmaI XmnI ZraI