(Linear) (Six-base) MAPSORT of: KIBB3277.seq Check: 5355 from: 1 to: 1815 KIBB3277.orf With 182 enzymes: SgfI * April 12, 2016 09:51 .. Acc65I G'GTAC_C Cuts at: 0 666 1815 Size: 666 1149 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 727 1403 1815 Size: 727 676 412 Fragments arranged by size: 727 676 412 AfeI AGC'GCT Cuts at: 0 743 1815 Size: 743 1072 AhdI GACnn_n'nnGTC Cuts at: 0 685 1815 Size: 685 1130 AlwNI CAG_nnn'CTG Cuts at: 0 241 1815 Size: 241 1574 ApaI G_GGCC'C Cuts at: 0 700 1815 Size: 700 1115 ApoI r'AATT_y Cuts at: 0 292 415 441 1815 Size: 292 123 26 1374 Fragments arranged by size: 1374 292 123 26 AseI AT'TA_AT Cuts at: 0 968 1815 Size: 968 847 AvaI C'yCGr_G Cuts at: 0 1064 1661 1815 Size: 1064 597 154 Fragments arranged by size: 1064 597 154 BanI G'GyrC_C Cuts at: 0 666 1652 1815 Size: 666 986 163 Fragments arranged by size: 986 666 163 BanII G_rGCy'C Cuts at: 0 700 1815 Size: 700 1115 BbsI GAAGACnn'nnnn_ Cuts at: 0 643 1815 Size: 643 1172 BbvCI CC'TCA_GC Cuts at: 0 1437 1815 Size: 1437 378 BciVI GTATCCnnnnn_n' Cuts at: 0 954 1815 Size: 954 861 BclI T'GATC_A Cuts at: 0 1335 1815 Size: 1335 480 BlpI GC'TnA_GC Cuts at: 0 885 1815 Size: 885 930 Bme1580I G_kGCm'C Cuts at: 0 700 1815 Size: 700 1115 BmgBI CAC'GTC Cuts at: 0 1668 1815 Size: 1668 147 BmrI ACTGGGnnnn_n' Cuts at: 0 355 572 836 1815 Size: 355 217 264 979 Fragments arranged by size: 979 355 264 217 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 118 408 1118 1225 1467 1815 Size: 118 290 710 107 242 348 Fragments arranged by size: 710 348 290 242 118 107 Bpu10I CC'TnA_GC Cuts at: 0 1261 1437 1815 Size: 1261 176 378 Fragments arranged by size: 1261 378 176 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1061 1192 1313 1815 Size: 1061 131 121 502 Fragments arranged by size: 1061 502 131 121 BsaI GGTCTCn'nnnn_ Cuts at: 0 1606 1815 Size: 1606 209 BsaAI yAC'GTr Cuts at: 0 1536 1569 1815 Size: 1536 33 246 Fragments arranged by size: 1536 246 33 BsaBI GATnn'nnATC Cuts at: 0 784 1815 Size: 784 1031 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 550 580 1215 1245 1815 Size: 550 30 635 30 570 Fragments arranged by size: 635 570 550 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 115 641 1425 1815 Size: 115 526 784 390 Fragments arranged by size: 784 526 390 115 BseYI C'CCAG_C Cuts at: 0 1234 1418 1780 1815 Size: 1234 184 362 35 Fragments arranged by size: 1234 362 184 35 BsiHKAI G_wGCw'C Cuts at: 0 647 1190 1815 Size: 647 543 625 Fragments arranged by size: 647 625 543 BsmI GAATG_Cn' Cuts at: 0 626 1815 Size: 626 1189 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1601 1815 Size: 1601 214 Bsp1286I G_dGCh'C Cuts at: 0 647 700 1190 1815 Size: 647 53 490 625 Fragments arranged by size: 647 625 490 53 BsrBI CCG'CTC Cuts at: 0 135 566 1357 1427 1815 Size: 135 431 791 70 388 Fragments arranged by size: 791 431 388 135 70 BsrDI GCAATG_nn' Cuts at: 0 934 1815 Size: 934 881 BsrFI r'CCGG_y Cuts at: 0 26 1815 Size: 26 1789 BstBI TT'CG_AA Cuts at: 0 260 1815 Size: 260 1555 BstEII G'GTnAC_C Cuts at: 0 795 1815 Size: 795 1020 BstXI CCAn_nnnn'nTGG Cuts at: 0 1462 1815 Size: 1462 353 BstYI r'GATC_y Cuts at: 0 865 1815 Size: 865 950 Bsu36I CC'TnA_GG Cuts at: 0 1037 1815 Size: 1037 778 BtgI C'CryG_G Cuts at: 0 1617 1815 Size: 1617 198 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 7 1441 1815 Size: 7 1434 374 Fragments arranged by size: 1434 374 7 BtsI GCAGTG_nn' Cuts at: 0 10 122 1467 1632 1815 Size: 10 112 1345 165 183 Fragments arranged by size: 1345 183 165 112 10 DraI TTT'AAA Cuts at: 0 758 1815 Size: 758 1057 DraIII CAC_nnn'GTG Cuts at: 0 1591 1815 Size: 1591 224 EaeI y'GGCC_r Cuts at: 0 248 1815 Size: 248 1567 EarI CTCTTCn'nnn_ Cuts at: 0 1434 1815 Size: 1434 381 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 792 1798 1815 Size: 792 1006 17 Fragments arranged by size: 1006 792 17 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 118 408 727 1118 1225 1403 1467 1815 Size: 118 290 319 391 107 178 64 348 Fragments arranged by size: 391 348 319 290 178 118 107 64 EcoO109I rG'GnC_Cy Cuts at: 0 697 1815 Size: 697 1118 EcoRV GAT'ATC Cuts at: 0 782 1760 1815 Size: 782 978 55 Fragments arranged by size: 978 782 55 FspI TGC'GCA Cuts at: 0 121 1815 Size: 121 1694 HaeII r_GCGC'y Cuts at: 0 745 1815 Size: 745 1070 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 671 703 1017 1049 1332 1364 1815 Size: 671 32 314 32 283 32 451 Fragments arranged by size: 671 451 314 283 32 32 32 HindIII A'AGCT_T Cuts at: 0 882 1815 Size: 882 933 KpnI G_GTAC'C Cuts at: 0 670 1815 Size: 670 1145 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 134 893 1815 Size: 134 759 922 Fragments arranged by size: 922 759 134 MscI TGG'CCA Cuts at: 0 250 1815 Size: 250 1565 MslI CAynn'nnrTG Cuts at: 0 224 727 1460 1815 Size: 224 503 733 355 Fragments arranged by size: 733 503 355 224 MspA1I CmG'CkG Cuts at: 0 802 1111 1815 Size: 802 309 704 Fragments arranged by size: 802 704 309 NaeI GCC'GGC Cuts at: 0 28 1815 Size: 28 1787 NgoMIV G'CCGG_C Cuts at: 0 26 1815 Size: 26 1789 NspI r_CATG'y Cuts at: 0 283 1815 Size: 283 1532 PflMI CCAn_nnn'nTGG Cuts at: 0 241 1815 Size: 241 1574 PmlI CAC'GTG Cuts at: 0 1569 1815 Size: 1569 246 PshAI GACnn'nnGTC Cuts at: 0 199 1815 Size: 199 1616 PspOMI G'GGCC_C Cuts at: 0 696 1815 Size: 696 1119 PstI C_TGCA'G Cuts at: 0 389 1815 Size: 389 1426 PvuII CAG'CTG Cuts at: 0 1111 1815 Size: 1111 704 SapI GCTCTTCn'nnn_ Cuts at: 0 1434 1815 Size: 1434 381 SfcI C'TryA_G Cuts at: 0 207 385 506 1815 Size: 207 178 121 1309 Fragments arranged by size: 1309 207 178 121 SgrAI Cr'CCGG_yG Cuts at: 0 26 1815 Size: 26 1789 SmaI CCC'GGG Cuts at: 0 1663 1815 Size: 1663 152 SmlI C'TyrA_G Cuts at: 0 1064 1076 1171 1328 1815 Size: 1064 12 95 157 487 Fragments arranged by size: 1064 487 157 95 12 SnaBI TAC'GTA Cuts at: 0 1536 1815 Size: 1536 279 StuI AGG'CCT Cuts at: 0 1210 1815 Size: 1210 605 StyI C'CwwG_G Cuts at: 0 998 1724 1815 Size: 998 726 91 Fragments arranged by size: 998 726 91 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 448 1240 1815 Size: 448 792 575 Fragments arranged by size: 792 575 448 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 57 89 1215 1247 1414 1446 1630 1662 Size: 57 32 1126 32 167 32 184 32 Cuts at: 1662 1815 Size: 153 Fragments arranged by size: 1126 184 167 153 57 32 32 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 681 1815 Size: 681 1134 XhoI C'TCGA_G Cuts at: 0 1064 1815 Size: 1064 751 XmaI C'CCGG_G Cuts at: 0 1661 1815 Size: 1661 154 Enzymes that do cut: Acc65I AcuI AfeI AhdI AlwNI ApaI ApoI AseI AvaI BanI BanII BbsI BbvCI BciVI BclI BlpI Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaXI BseRI BseYI BsiHKAI BsmI BsmBI Bsp1286I BsrBI BsrDI BsrFI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII EaeI EarI EciI Eco57MI EcoO109I EcoRV FspI HaeII Hin4I HindIII KpnI MmeI MscI MslI MspA1I NaeI NgoMIV NspI PflMI PmlI PshAI PspOMI PstI PvuII SapI SfcI SgrAI SmaI SmlI SnaBI StuI StyI TaqII TstI XcmI XhoI XmaI Enzymes that do not cut: AarI AatII AccI AclI AflII AflIII AgeI AleI AlfI AloI ApaLI AscI AsiSI AvrII BaeI BamHI BbeI BcgI BfrBI BglI BglII BmtI BplI BsaHI BsaWI BsgI BsiEI BsiWI BspEI BspHI BspMI BsrGI BssHII BssSI BstAPI BstZ17I ClaI CspCI DrdI EagI EcoICRI EcoNI EcoRI FalI FseI FspAI HincII HpaI KasI MfeI MluI NarI NcoI NdeI NheI NotI NruI NsiI PacI PasI PciI PfoI PmeI PpiI PpuMI PsiI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI SpeI SphI SrfI SspI SwaI TatI Tth111I XbaI XmnI ZraI