Restriction map for
pF1KB3287
(Linear) (Six-base) MAPSORT of: KIBB3287.seq Check: 5279 from: 1 to: 2868
KIBB3287.orf
With 182 enzymes: SgfI *
February 28, 2007 11:24 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 706 1312 2356 2868
Size: 706 606 1044 512
Fragments arranged by size:
1044 706 606 512
AflIII A'CryG_T
Cuts at: 0 2078 2868
Size: 2078 790
AhdI GACnn_n'nnGTC
Cuts at: 0 564 2868
Size: 564 2304
AleI CACnn'nnGTG
Cuts at: 0 1733 2868
Size: 1733 1135
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1966 2868
Size: 1966 902
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1756 1788 2868
Size: 1756 32 1080
Fragments arranged by size:
1756 1080 32
AlwNI CAG_nnn'CTG
Cuts at: 0 963 1790 2712 2790 2868
Size: 963 827 922 78 78
Fragments arranged by size:
963 922 827 78 78
ApoI r'AATT_y
Cuts at: 0 1090 2040 2142 2868
Size: 1090 950 102 726
Fragments arranged by size:
1090 950 726 102
AscI GG'CGCG_CC
Cuts at: 0 2592 2868
Size: 2592 276
AvaI C'yCGr_G
Cuts at: 0 590 1426 2626 2767 2863 2868
Size: 590 836 1200 141 96 5
Fragments arranged by size:
1200 836 590 141 96 5
BamHI G'GATC_C
Cuts at: 0 455 2868
Size: 455 2413
BanI G'GyrC_C
Cuts at: 0 15 1030 1438 2868
Size: 15 1015 408 1430
Fragments arranged by size:
1430 1015 408 15
BanII G_rGCy'C
Cuts at: 0 1825 2693 2864 2868
Size: 1825 868 171 4
Fragments arranged by size:
1825 868 171 4
BbeI G_GCGC'C
Cuts at: 0 19 2868
Size: 19 2849
BbvCI CC'TCA_GC
Cuts at: 0 1192 2868
Size: 1192 1676
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2244 2278 2786 2820 2868
Size: 2244 34 508 34 48
Fragments arranged by size:
2244 508 48 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 2584 2868
Size: 2584 284
BclI T'GATC_A
Cuts at: 0 1889 2868
Size: 1889 979
BglI GCCn_nnn'nGGC
Cuts at: 0 1666 1918 2613 2734 2868
Size: 1666 252 695 121 134
Fragments arranged by size:
1666 695 252 134 121
BglII A'GATC_T
Cuts at: 0 1016 1482 2868
Size: 1016 466 1386
Fragments arranged by size:
1386 1016 466
BlpI GC'TnA_GC
Cuts at: 0 2659 2868
Size: 2659 209
Bme1580I G_kGCm'C
Cuts at: 0 890 1740 1955 2368 2650 2799 2868
Size: 890 850 215 413 282 149 69
Fragments arranged by size:
890 850 413 282 215 149 69
BmrI ACTGGGnnnn_n'
Cuts at: 0 905 2868
Size: 905 1963
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 144 2868
Size: 144 2724
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 291 739 763 2004 2287 2347 2851 2868
Size: 291 448 24 1241 283 60 504 17
Fragments arranged by size:
1241 504 448 291 283 60 24 17
Bpu10I CC'TnA_GC
Cuts at: 0 1192 2868
Size: 1192 1676
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 93 595 1339 2868
Size: 93 502 744 1529
Fragments arranged by size:
1529 744 502 93
BsaAI yAC'GTr
Cuts at: 0 1179 2868
Size: 1179 1689
BsaBI GATnn'nnATC
Cuts at: 0 1894 2868
Size: 1894 974
BsaHI Gr'CG_yC
Cuts at: 0 16 1637 1705 1868 2868
Size: 16 1621 68 163 1000
Fragments arranged by size:
1621 1000 163 68 16
BsaWI w'CCGG_w
Cuts at: 0 968 1797 2868
Size: 968 829 1071
Fragments arranged by size:
1071 968 829
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1756 1786 2002 2032 2868
Size: 1756 30 216 30 836
Fragments arranged by size:
1756 836 216 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 385 598 900 2134 2644 2836 2868
Size: 385 213 302 1234 510 192 32
Fragments arranged by size:
1234 510 385 302 213 192 32
BseYI C'CCAG_C
Cuts at: 0 174 300 421 451 2095 2868
Size: 174 126 121 30 1644 773
Fragments arranged by size:
1644 773 174 126 121 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 129 459 976 1957 2730 2868
Size: 129 330 517 981 773 138
Fragments arranged by size:
981 773 517 330 138 129
BsiEI CG_ry'CG
Cuts at: 0 1802 2641 2803 2868
Size: 1802 839 162 65
Fragments arranged by size:
1802 839 162 65
BsiHKAI G_wGCw'C
Cuts at: 0 2116 2868
Size: 2116 752
BsmI GAATG_Cn'
Cuts at: 0 514 1568 1879 2868
Size: 514 1054 311 989
Fragments arranged by size:
1054 989 514 311
BsmBI CGTCTCn'nnnn_
Cuts at: 0 15 2868
Size: 15 2853
Bsp1286I G_dGCh'C
Cuts at: 0 890 1740 1825 1955 2116 2368 2650 2693
Size: 890 850 85 130 161 252 282 43
Cuts at: 2693 2799 2864 2868
Size: 106 65 4
Fragments arranged by size:
890 850 282 252 161 130 106 85
65 43 4
BspHI T'CATG_A
Cuts at: 0 1040 1550 2868
Size: 1040 510 1318
Fragments arranged by size:
1318 1040 510
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1058 2868
Size: 1058 1810
BsrBI CCG'CTC
Cuts at: 0 123 606 2868
Size: 123 483 2262
Fragments arranged by size:
2262 483 123
BsrDI GCAATG_nn'
Cuts at: 0 1326 2868
Size: 1326 1542
BsrFI r'CCGG_y
Cuts at: 0 1078 1745 2585 2868
Size: 1078 667 840 283
Fragments arranged by size:
1078 840 667 283
BssHII G'CGCG_C
Cuts at: 0 2592 2868
Size: 2592 276
BssSI C'ACGA_G
Cuts at: 0 43 2868
Size: 43 2825
BstEII G'GTnAC_C
Cuts at: 0 2535 2868
Size: 2535 333
BstXI CCAn_nnnn'nTGG
Cuts at: 0 138 270 1269 2463 2868
Size: 138 132 999 1194 405
Fragments arranged by size:
1194 999 405 138 132
BstYI r'GATC_y
Cuts at: 0 254 455 617 1016 1370 1391 1482 2330
Size: 254 201 162 399 354 21 91 848
Cuts at: 2330 2868
Size: 538
Fragments arranged by size:
848 538 399 354 254 201 162 91
21
BtgI C'CryG_G
Cuts at: 0 101 2641 2739 2868
Size: 101 2540 98 129
Fragments arranged by size:
2540 129 101 98
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1815 2868
Size: 1815 1053
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 312 347 2050 2085 2868
Size: 312 35 1703 35 783
Fragments arranged by size:
1703 783 312 35 35
DraI TTT'AAA
Cuts at: 0 518 2868
Size: 518 2350
EaeI y'GGCC_r
Cuts at: 0 140 1080 1167 1667 1910 2294 2453 2800
Size: 140 940 87 500 243 384 159 347
Cuts at: 2800 2868
Size: 68
Fragments arranged by size:
940 500 384 347 243 159 140 87
68
EagI C'GGCC_G
Cuts at: 0 2800 2868
Size: 2800 68
EarI CTCTTCn'nnn_
Cuts at: 0 1243 1651 1690 2104 2868
Size: 1243 408 39 414 764
Fragments arranged by size:
1243 764 414 408 39
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1384 2868
Size: 1384 1484
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 291 706 739 763 1312 2004 2287 2347
Size: 291 415 33 24 549 692 283 60
Cuts at: 2347 2356 2851 2868
Size: 9 495 17
Fragments arranged by size:
692 549 495 415 291 283 60 33
24 17 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 262 685 2563 2868
Size: 262 423 1878 305
Fragments arranged by size:
1878 423 305 262
EcoO109I rG'GnC_Cy
Cuts at: 0 345 594 1188 2223 2787 2868
Size: 345 249 594 1035 564 81
Fragments arranged by size:
1035 594 564 345 249 81
EcoRI G'AATT_C
Cuts at: 0 1090 2142 2868
Size: 1090 1052 726
Fragments arranged by size:
1090 1052 726
FspI TGC'GCA
Cuts at: 0 1054 2548 2868
Size: 1054 1494 320
Fragments arranged by size:
1494 1054 320
HaeII r_GCGC'y
Cuts at: 0 19 2637 2868
Size: 19 2618 231
Fragments arranged by size:
2618 231 19
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 550 582 2868
Size: 550 32 2286
Fragments arranged by size:
2286 550 32
HincII GTy'rAC
Cuts at: 0 789 1703 2868
Size: 789 914 1165
Fragments arranged by size:
1165 914 789
KasI G'GCGC_C
Cuts at: 0 15 2868
Size: 15 2853
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1139 1244 2171 2453 2868
Size: 1139 105 927 282 415
Fragments arranged by size:
1139 927 415 282 105
MscI TGG'CCA
Cuts at: 0 142 2296 2455 2868
Size: 142 2154 159 413
Fragments arranged by size:
2154 413 159 142
MslI CAynn'nnrTG
Cuts at: 0 439 1211 1731 1733 1948 2461 2868
Size: 439 772 520 2 215 513 407
Fragments arranged by size:
772 520 513 439 407 215 2
MspA1I CmG'CkG
Cuts at: 0 1513 1676 1856 2307 2643 2741 2868
Size: 1513 163 180 451 336 98 127
Fragments arranged by size:
1513 451 336 180 163 127 98
NaeI GCC'GGC
Cuts at: 0 2587 2868
Size: 2587 281
NarI GG'CG_CC
Cuts at: 0 16 2868
Size: 16 2852
NcoI C'CATG_G
Cuts at: 0 101 2868
Size: 101 2767
NdeI CA'TA_TG
Cuts at: 0 1586 2868
Size: 1586 1282
NgoMIV G'CCGG_C
Cuts at: 0 2585 2868
Size: 2585 283
NspI r_CATG'y
Cuts at: 0 579 1353 2070 2082 2259 2868
Size: 579 774 717 12 177 609
Fragments arranged by size:
774 717 609 579 177 12
PasI CC'CwG_GG
Cuts at: 0 1740 1776 2868
Size: 1740 36 1092
Fragments arranged by size:
1740 1092 36
PciI A'CATG_T
Cuts at: 0 2078 2868
Size: 2078 790
PflMI CCAn_nnn'nTGG
Cuts at: 0 762 1311 2532 2868
Size: 762 549 1221 336
Fragments arranged by size:
1221 762 549 336
PfoI T'CCnGG_A
Cuts at: 0 620 1268 1616 2162 2276 2868
Size: 620 648 348 546 114 592
Fragments arranged by size:
648 620 592 546 348 114
PmlI CAC'GTG
Cuts at: 0 1179 2868
Size: 1179 1689
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1756 1788 2868
Size: 1756 32 1080
Fragments arranged by size:
1756 1080 32
PpuMI rG'GwC_Cy
Cuts at: 0 345 594 2868
Size: 345 249 2274
Fragments arranged by size:
2274 345 249
PshAI GACnn'nnGTC
Cuts at: 0 291 2868
Size: 291 2577
PstI C_TGCA'G
Cuts at: 0 61 240 2312 2868
Size: 61 179 2072 556
Fragments arranged by size:
2072 556 179 61
PvuII CAG'CTG
Cuts at: 0 1513 1676 2307 2868
Size: 1513 163 631 561
Fragments arranged by size:
1513 631 561 163
SacII CC_GC'GG
Cuts at: 0 2644 2742 2868
Size: 2644 98 126
Fragments arranged by size:
2644 126 98
SanDI GG'GwC_CC
Cuts at: 0 594 2868
Size: 594 2274
SapI GCTCTTCn'nnn_
Cuts at: 0 1651 2104 2868
Size: 1651 453 764
Fragments arranged by size:
1651 764 453
ScaI AGT'ACT
Cuts at: 0 1771 2185 2868
Size: 1771 414 683
Fragments arranged by size:
1771 683 414
SfcI C'TryA_G
Cuts at: 0 57 236 446 2308 2868
Size: 57 179 210 1862 560
Fragments arranged by size:
1862 560 210 179 57
SfoI GGC'GCC
Cuts at: 0 17 2868
Size: 17 2851
SgrAI Cr'CCGG_yG
Cuts at: 0 2585 2868
Size: 2585 283
SmaI CCC'GGG
Cuts at: 0 592 2868
Size: 592 2276
SmlI C'TyrA_G
Cuts at: 0 72 610 1354 2868
Size: 72 538 744 1514
Fragments arranged by size:
1514 744 538 72
SphI G_CATG'C
Cuts at: 0 579 2259 2868
Size: 579 1680 609
Fragments arranged by size:
1680 609 579
SspI AAT'ATT
Cuts at: 0 2412 2868
Size: 2412 456
StuI AGG'CCT
Cuts at: 0 1499 2396 2868
Size: 1499 897 472
Fragments arranged by size:
1499 897 472
StyI C'CwwG_G
Cuts at: 0 101 185 263 554 2237 2369 2868
Size: 101 84 78 291 1683 132 499
Fragments arranged by size:
1683 499 291 132 101 84 78
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1745 2554 2868
Size: 1745 809 314
Fragments arranged by size:
1745 809 314
TatI w'GTAC_w
Cuts at: 0 1769 2183 2868
Size: 1769 414 685
Fragments arranged by size:
1769 685 414
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2002 2034 2411 2443 2868
Size: 2002 32 377 32 425
Fragments arranged by size:
2002 425 377 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 192 1962 2868
Size: 192 1770 906
Fragments arranged by size:
1770 906 192
XmaI C'CCGG_G
Cuts at: 0 590 2868
Size: 590 2278
XmnI GAAnn'nnTTC
Cuts at: 0 466 2868
Size: 466 2402
Enzymes that do cut:
AcuI AflIII AhdI AleI AlfI AloI AlwNI ApoI
AscI AvaI BamHI BanI BanII BbeI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstEII
BstXI BstYI BtgI BtgZI CspCI DraI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII KasI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NspI PasI PciI PflMI
PfoI PmlI PpiI PpuMI PshAI PstI PvuII SacII
SanDI SapI ScaI SfcI SfoI SgrAI SmaI SmlI
SphI SspI StuI StyI TaqII TatI TstI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
ApaI ApaLI AseI AsiSI AvrII BaeI BbsI BfrBI
BmgBI BmtI BsaI BsiWI BspEI BsrGI BstAPI BstBI
BstZ17I Bsu36I BtsI ClaI DraIII DrdI EcoICRI EcoRV
FalI FseI FspAI HindIII HpaI KpnI MfeI MluI
NheI NotI NruI NsiI PacI PmeI PsiI PspOMI
PspXI PsrI PvuI RsrII SacI SalI SbfI SexAI
SfiI SgfI SnaBI SpeI SrfI SwaI Tth111I XbaI
XhoI ZraI