Restriction map for
pF1KB3288
(Linear) (Six-base) MAPSORT of: KIBB3288.seq Check: 1191 from: 1 to: 2232
KIBB3288.orf
With 182 enzymes: SgfI *
July 18, 2007 13:36 ..
AccI GT'mk_AC
Cuts at: 0 1979 2232
Size: 1979 253
Acc65I G'GTAC_C
Cuts at: 0 906 2232
Size: 906 1326
AflIII A'CryG_T
Cuts at: 0 2018 2060 2232
Size: 2018 42 172
Fragments arranged by size:
2018 172 42
AgeI A'CCGG_T
Cuts at: 0 1881 2232
Size: 1881 351
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 235 2232
Size: 235 1997
AlwNI CAG_nnn'CTG
Cuts at: 0 30 159 2232
Size: 30 129 2073
Fragments arranged by size:
2073 129 30
ApaI G_GGCC'C
Cuts at: 0 401 845 853 1207 1379 1486 1513 1577
Size: 401 444 8 354 172 107 27 64
Cuts at: 1577 1893 2232
Size: 316 339
Fragments arranged by size:
444 401 354 339 316 172 107 64
27 8
ApoI r'AATT_y
Cuts at: 0 116 140 275 2232
Size: 116 24 135 1957
Fragments arranged by size:
1957 135 116 24
BamHI G'GATC_C
Cuts at: 0 572 965 2232
Size: 572 393 1267
Fragments arranged by size:
1267 572 393
BanI G'GyrC_C
Cuts at: 0 82 285 906 1141 1642 2232
Size: 82 203 621 235 501 590
Fragments arranged by size:
621 590 501 235 203 82
BanII G_rGCy'C
Cuts at: 0 401 845 853 873 1110 1207 1379 1486
Size: 401 444 8 20 237 97 172 107
Cuts at: 1486 1513 1577 1893 2051 2232
Size: 27 64 316 158 181
Fragments arranged by size:
444 401 316 237 181 172 158 107
97 64 27 20 8
BbeI G_GCGC'C
Cuts at: 0 289 2232
Size: 289 1943
BciVI GTATCCnnnnn_n'
Cuts at: 0 257 416 926 1504 2232
Size: 257 159 510 578 728
Fragments arranged by size:
728 578 510 257 159
BglI GCCn_nnn'nGGC
Cuts at: 0 633 788 1266 1517 1667 1804 2232
Size: 633 155 478 251 150 137 428
Fragments arranged by size:
633 478 428 251 155 150 137
Bme1580I G_kGCm'C
Cuts at: 0 401 845 853 1146 1207 1379 1486 1513
Size: 401 444 8 293 61 172 107 27
Cuts at: 1513 1577 1647 1893 2232
Size: 64 70 246 339
Fragments arranged by size:
444 401 339 293 246 172 107 70
64 61 27 8
BmrI ACTGGGnnnn_n'
Cuts at: 0 1888 2232
Size: 1888 344
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 578 752 857 1148 1193 1676 1685 2131
Size: 578 174 105 291 45 483 9 446
Cuts at: 2131 2232
Size: 101
Fragments arranged by size:
578 483 446 291 174 105 101 45
9
Bpu10I CC'TnA_GC
Cuts at: 0 2122 2232
Size: 2122 110
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 683 1640 2232
Size: 683 957 592
Fragments arranged by size:
957 683 592
BsaI GGTCTCn'nnnn_
Cuts at: 0 2126 2232
Size: 2126 106
BsaAI yAC'GTr
Cuts at: 0 2063 2232
Size: 2063 169
BsaHI Gr'CG_yC
Cuts at: 0 286 2232
Size: 286 1946
BsaWI w'CCGG_w
Cuts at: 0 1881 2232
Size: 1881 351
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1397 1427 2123 2153 2232
Size: 1397 30 696 30 79
Fragments arranged by size:
1397 696 79 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 113 2190 2232
Size: 113 2077 42
Fragments arranged by size:
2077 113 42
BseYI C'CCAG_C
Cuts at: 0 30 1390 1710 1767 2232
Size: 30 1360 320 57 465
Fragments arranged by size:
1360 465 320 57 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2129 2232
Size: 2129 103
BsiWI C'GTAC_G
Cuts at: 0 2063 2232
Size: 2063 169
BsmI GAATG_Cn'
Cuts at: 0 497 2232
Size: 497 1735
Bsp1286I G_dGCh'C
Cuts at: 0 401 845 853 873 1110 1146 1207 1379
Size: 401 444 8 20 237 36 61 172
Cuts at: 1379 1486 1513 1577 1647 1893 2051 2232
Size: 107 27 64 70 246 158 181
Fragments arranged by size:
444 401 246 237 181 172 158 107
70 64 61 36 27 20 8
BsrFI r'CCGG_y
Cuts at: 0 282 1881 2232
Size: 282 1599 351
Fragments arranged by size:
1599 351 282
BsrGI T'GTAC_A
Cuts at: 0 2057 2232
Size: 2057 175
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1011 2232
Size: 1011 1221
BstEII G'GTnAC_C
Cuts at: 0 641 1078 2232
Size: 641 437 1154
Fragments arranged by size:
1154 641 437
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2101 2232
Size: 2101 131
BstYI r'GATC_y
Cuts at: 0 572 965 2232
Size: 572 393 1267
Fragments arranged by size:
1267 572 393
Bsu36I CC'TnA_GG
Cuts at: 0 1442 2232
Size: 1442 790
BtgI C'CryG_G
Cuts at: 0 206 366 512 2232
Size: 206 160 146 1720
Fragments arranged by size:
1720 206 160 146
BtsI GCAGTG_nn'
Cuts at: 0 2001 2117 2232
Size: 2001 116 115
Fragments arranged by size:
2001 116 115
EaeI y'GGCC_r
Cuts at: 0 188 2232
Size: 188 2044
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 578 752 857 1148 1193 1676 1685 2131
Size: 578 174 105 291 45 483 9 446
Cuts at: 2131 2232
Size: 101
Fragments arranged by size:
578 483 446 291 174 105 101 45
9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1592 2092 2232
Size: 1592 500 140
Fragments arranged by size:
1592 500 140
EcoO109I rG'GnC_Cy
Cuts at: 0 361 398 589 598 676 694 712 763
Size: 361 37 191 9 78 18 18 51
Cuts at: 763 842 849 850 895 922 1027 1069 1159
Size: 79 7 1 45 27 105 42 90
Cuts at: 1159 1195 1204 1276 1339 1366 1375 1482 1483
Size: 36 9 72 63 27 9 107 1
Cuts at: 1483 1509 1510 1546 1573 1574 1678 1687 1696
Size: 26 1 36 27 1 104 9 9
Cuts at: 1696 1705 1889 1890 1968 2232
Size: 9 184 1 78 264
Fragments arranged by size:
361 264 191 184 107 105 104 90
79 78 78 72 63 51 45 42
37 36 36 27 27 27 26 18
18 9 9 9 9 9 9 7
1 1 1 1 1
EcoRV GAT'ATC
Cuts at: 0 460 2232
Size: 460 1772
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 217 2232
Size: 217 2015
HaeII r_GCGC'y
Cuts at: 0 289 2232
Size: 289 1943
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 2083 2115 2232
Size: 2083 32 117
Fragments arranged by size:
2083 117 32
KasI G'GCGC_C
Cuts at: 0 285 2232
Size: 285 1947
KpnI G_GTAC'C
Cuts at: 0 910 2232
Size: 910 1322
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 450 777 2232
Size: 450 327 1455
Fragments arranged by size:
1455 450 327
MscI TGG'CCA
Cuts at: 0 190 2232
Size: 190 2042
MslI CAynn'nnrTG
Cuts at: 0 517 2232
Size: 517 1715
MspA1I CmG'CkG
Cuts at: 0 27 105 1714 2232
Size: 27 78 1609 518
Fragments arranged by size:
1609 518 78 27
NarI GG'CG_CC
Cuts at: 0 286 2232
Size: 286 1946
NcoI C'CATG_G
Cuts at: 0 206 512 2232
Size: 206 306 1720
Fragments arranged by size:
1720 306 206
NspI r_CATG'y
Cuts at: 0 183 522 2232
Size: 183 339 1710
Fragments arranged by size:
1710 339 183
PasI CC'CwG_GG
Cuts at: 0 159 838 846 855 1462 1498 1570 1579
Size: 159 679 8 9 607 36 72 9
Cuts at: 1579 1630 1740 2232
Size: 51 110 492
Fragments arranged by size:
679 607 492 159 110 72 51 36
9 9 8
PflMI CCAn_nnn'nTGG
Cuts at: 0 159 418 469 1048 1504 2232
Size: 159 259 51 579 456 728
Fragments arranged by size:
728 579 456 259 159 51
PfoI T'CCnGG_A
Cuts at: 0 462 873 1164 1227 1674 1692 1701 2087
Size: 462 411 291 63 447 18 9 386
Cuts at: 2087 2232
Size: 145
Fragments arranged by size:
462 447 411 386 291 145 63 18
9
PpuMI rG'GwC_Cy
Cuts at: 0 361 589 676 694 712 922 1027 1069
Size: 361 228 87 18 18 210 105 42
Cuts at: 1069 1159 1195 1276 1366 1678 1696 1705 1968
Size: 90 36 81 90 312 18 9 263
Cuts at: 1968 2232
Size: 264
Fragments arranged by size:
361 312 264 263 228 210 105 90
90 87 81 42 36 18 18 18
9
PspOMI G'GGCC_C
Cuts at: 0 397 841 849 1203 1375 1482 1509 1573
Size: 397 444 8 354 172 107 27 64
Cuts at: 1573 1889 2232
Size: 316 343
Fragments arranged by size:
444 397 354 343 316 172 107 64
27 8
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 899 931 2232
Size: 899 32 1301
Fragments arranged by size:
1301 899 32
PstI C_TGCA'G
Cuts at: 0 26 2172 2232
Size: 26 2146 60
Fragments arranged by size:
2146 60 26
PvuII CAG'CTG
Cuts at: 0 27 1714 2232
Size: 27 1687 518
Fragments arranged by size:
1687 518 27
SexAI A'CCwGG_T
Cuts at: 0 1272 2232
Size: 1272 960
SfcI C'TryA_G
Cuts at: 0 22 2168 2232
Size: 22 2146 64
Fragments arranged by size:
2146 64 22
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 788 1266 1517 1667 2232
Size: 788 478 251 150 565
Fragments arranged by size:
788 565 478 251 150
SfoI GGC'GCC
Cuts at: 0 287 2232
Size: 287 1945
SgrAI Cr'CCGG_yG
Cuts at: 0 1881 2232
Size: 1881 351
SmlI C'TyrA_G
Cuts at: 0 698 1655 2232
Size: 698 957 577
Fragments arranged by size:
957 698 577
SphI G_CATG'C
Cuts at: 0 522 2232
Size: 522 1710
StuI AGG'CCT
Cuts at: 0 704 1037 1178 2232
Size: 704 333 141 1054
Fragments arranged by size:
1054 704 333 141
StyI C'CwwG_G
Cuts at: 0 206 512 1280 1326 1379 1647 2232
Size: 206 306 768 46 53 268 585
Fragments arranged by size:
768 585 306 268 206 53 46
TatI w'GTAC_w
Cuts at: 0 2057 2072 2232
Size: 2057 15 160
Fragments arranged by size:
2057 160 15
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 615 647 729 761 1626 1658 2123 2155
Size: 615 32 82 32 865 32 465 32
Cuts at: 2155 2232
Size: 77
Fragments arranged by size:
865 615 465 82 77 32 32 32
32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 232 2232
Size: 232 2000
Enzymes that do cut:
AccI Acc65I AflIII AgeI AlfI AlwNI ApaI ApoI
BamHI BanI BanII BbeI BciVI BglI Bme1580I BmrI
BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiWI BsmI Bsp1286I BsrFI BsrGI
BstAPI BstEII BstXI BstYI Bsu36I BtgI BtsI EaeI
Eco57MI EcoNI EcoO109I EcoRV FalI HaeII Hin4I KasI
KpnI MmeI MscI MslI MspA1I NarI NcoI NspI
PasI PflMI PfoI PpuMI PspOMI PsrI PstI PvuII
SexAI SfcI SfiI SfoI SgrAI SmlI SphI StuI
StyI TatI TstI XcmI
Enzymes that do not cut:
AarI AatII AclI AcuI AfeI AflII AhdI AleI
AloI ApaLI AscI AseI AsiSI AvaI AvrII BaeI
BbsI BbvCI BcgI BclI BfrBI BglII BlpI BmgBI
BmtI BplI BsaBI BsiEI BsiHKAI BsmBI BspEI BspHI
BspMI BsrBI BsrDI BssHII BssSI BstBI BstZ17I BtgZI
ClaI CspCI DraI DraIII DrdI EagI EarI EciI
EcoICRI EcoRI FseI FspI FspAI HincII HindIII HpaI
MfeI MluI NaeI NdeI NgoMIV NheI NotI NruI
NsiI PacI PciI PmeI PmlI PpiI PshAI PsiI
PspXI PvuI RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SgfI SmaI SnaBI SpeI SrfI SspI
SwaI TaqII Tth111I XbaI XhoI XmaI XmnI ZraI