Restriction map for
pF1KB3300
(Linear) (Six-base) MAPSORT of: KIBB3300.seq Check: 6380 from: 1 to: 2169
KIBB3300.orf
With 182 enzymes: SgfI *
September 18, 2009 17:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2122 2169
Size: 2122 47
AccI GT'mk_AC
Cuts at: 0 557 2169
Size: 557 1612
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 436 838 871 1003 2169
Size: 436 402 33 132 1166
Fragments arranged by size:
1166 436 402 132 33
AfeI AGC'GCT
Cuts at: 0 1522 2169
Size: 1522 647
AhdI GACnn_n'nnGTC
Cuts at: 0 1491 2169
Size: 1491 678
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 178 326 1379 2169
Size: 178 148 1053 790
Fragments arranged by size:
1053 790 178 148
AlwNI CAG_nnn'CTG
Cuts at: 0 504 927 1023 1176 1293 1338 1551 1846
Size: 504 423 96 153 117 45 213 295
Cuts at: 1846 1884 2106 2169
Size: 38 222 63
Fragments arranged by size:
504 423 295 222 213 153 117 96
63 45 38
ApaI G_GGCC'C
Cuts at: 0 713 1530 2169
Size: 713 817 639
Fragments arranged by size:
817 713 639
AvaI C'yCGr_G
Cuts at: 0 692 802 2169
Size: 692 110 1367
Fragments arranged by size:
1367 692 110
BamHI G'GATC_C
Cuts at: 0 1013 1901 2169
Size: 1013 888 268
Fragments arranged by size:
1013 888 268
BanI G'GyrC_C
Cuts at: 0 580 815 2169
Size: 580 235 1354
Fragments arranged by size:
1354 580 235
BanII G_rGCy'C
Cuts at: 0 122 713 1530 1586 1813 2169
Size: 122 591 817 56 227 356
Fragments arranged by size:
817 591 356 227 122 56
BbeI G_GCGC'C
Cuts at: 0 819 2169
Size: 819 1350
BbvCI CC'TCA_GC
Cuts at: 0 1531 2169
Size: 1531 638
BfrBI ATG'CAT
Cuts at: 0 1401 2169
Size: 1401 768
BglII A'GATC_T
Cuts at: 0 258 2169
Size: 258 1911
BlpI GC'TnA_GC
Cuts at: 0 532 1748 1798 2169
Size: 532 1216 50 371
Fragments arranged by size:
1216 532 371 50
Bme1580I G_kGCm'C
Cuts at: 0 713 1530 2169
Size: 713 817 639
Fragments arranged by size:
817 713 639
BmgBI CAC'GTC
Cuts at: 0 1147 2076 2169
Size: 1147 929 93
Fragments arranged by size:
1147 929 93
BmrI ACTGGGnnnn_n'
Cuts at: 0 23 835 2169
Size: 23 812 1334
Fragments arranged by size:
1334 812 23
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1527 2169
Size: 1527 642
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 73 301 370 544 688 814 937 1363
Size: 73 228 69 174 144 126 123 426
Cuts at: 1363 1483 1528 1753 2023 2169
Size: 120 45 225 270 146
Fragments arranged by size:
426 270 228 225 174 146 144 126
123 120 73 69 45
Bpu10I CC'TnA_GC
Cuts at: 0 1531 2169
Size: 1531 638
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 711 2169
Size: 711 1458
BsaHI Gr'CG_yC
Cuts at: 0 542 816 1835 2169
Size: 542 274 1019 334
Fragments arranged by size:
1019 542 334 274
BsaWI w'CCGG_w
Cuts at: 0 14 1868 2169
Size: 14 1854 301
Fragments arranged by size:
1854 301 14
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 727 757 892 922 1141 1171 1726 1756
Size: 727 30 135 30 219 30 555 30
Cuts at: 1756 2169
Size: 413
Fragments arranged by size:
727 555 413 219 135 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 49 748 916 1138 1750 2169
Size: 49 699 168 222 612 419
Fragments arranged by size:
699 612 419 222 168 49
BseYI C'CCAG_C
Cuts at: 0 82 380 845 1523 2141 2169
Size: 82 298 465 678 618 28
Fragments arranged by size:
678 618 465 298 82 28
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2029 2169
Size: 2029 140
BsiHKAI G_wGCw'C
Cuts at: 0 122 842 938 1586 1754 2169
Size: 122 720 96 648 168 415
Fragments arranged by size:
720 648 415 168 122 96
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1842 2169
Size: 1842 327
Bsp1286I G_dGCh'C
Cuts at: 0 122 713 842 938 1530 1586 1754 1813
Size: 122 591 129 96 592 56 168 59
Cuts at: 1813 2169
Size: 356
Fragments arranged by size:
592 591 356 168 129 122 96 59
56
BspEI T'CCGG_A
Cuts at: 0 14 1868 2169
Size: 14 1854 301
Fragments arranged by size:
1854 301 14
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1766 1802 2122 2169
Size: 1766 36 320 47
Fragments arranged by size:
1766 320 47 36
BsrBI CCG'CTC
Cuts at: 0 6 960 1987 2169
Size: 6 954 1027 182
Fragments arranged by size:
1027 954 182 6
BsrFI r'CCGG_y
Cuts at: 0 583 1196 2169
Size: 583 613 973
Fragments arranged by size:
973 613 583
BssSI C'ACGA_G
Cuts at: 0 1858 2169
Size: 1858 311
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1173 1758 2169
Size: 1173 585 411
Fragments arranged by size:
1173 585 411
BstBI TT'CG_AA
Cuts at: 0 447 516 2169
Size: 447 69 1653
Fragments arranged by size:
1653 447 69
BstYI r'GATC_y
Cuts at: 0 258 482 881 1013 1334 1862 1901 2169
Size: 258 224 399 132 321 528 39 268
Fragments arranged by size:
528 399 321 268 258 224 132 39
BtgI C'CryG_G
Cuts at: 0 434 551 565 713 2169
Size: 434 117 14 148 1456
Fragments arranged by size:
1456 434 148 117 14
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1366 2121 2169
Size: 1366 755 48
Fragments arranged by size:
1366 755 48
BtsI GCAGTG_nn'
Cuts at: 0 587 2098 2104 2169
Size: 587 1511 6 65
Fragments arranged by size:
1511 587 65 6
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 453 488 2169
Size: 453 35 1681
Fragments arranged by size:
1681 453 35
EaeI y'GGCC_r
Cuts at: 0 548 593 1262 1823 2070 2169
Size: 548 45 669 561 247 99
Fragments arranged by size:
669 561 548 247 99 45
EarI CTCTTCn'nnn_
Cuts at: 0 13 766 1574 1694 2169
Size: 13 753 808 120 475
Fragments arranged by size:
808 753 475 120 13
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1149 2169
Size: 1149 1020
EcoICRI GAG'CTC
Cuts at: 0 120 1584 2169
Size: 120 1464 585
Fragments arranged by size:
1464 585 120
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 73 301 370 436 544 688 814 838
Size: 73 228 69 66 108 144 126 24
Cuts at: 838 871 937 1003 1363 1483 1528 1753 2023
Size: 33 66 66 360 120 45 225 270
Cuts at: 2023 2169
Size: 146
Fragments arranged by size:
360 270 228 225 146 144 126 120
108 73 69 66 66 66 45 33
24
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1732 1825 2169
Size: 1732 93 344
Fragments arranged by size:
1732 344 93
EcoO109I rG'GnC_Cy
Cuts at: 0 924 1527 1818 2169
Size: 924 603 291 351
Fragments arranged by size:
924 603 351 291
FspI TGC'GCA
Cuts at: 0 1772 2110 2169
Size: 1772 338 59
Fragments arranged by size:
1772 338 59
HaeII r_GCGC'y
Cuts at: 0 819 945 1524 1716 2148 2169
Size: 819 126 579 192 432 21
Fragments arranged by size:
819 579 432 192 126 21
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 492 524 709 741 892 924 1141 1173
Size: 492 32 185 32 151 32 217 32
Cuts at: 1173 1477 1509 1638 1670 1726 1758 2169
Size: 304 32 129 32 56 32 411
Fragments arranged by size:
492 411 304 217 185 151 129 56
32 32 32 32 32 32 32
KasI G'GCGC_C
Cuts at: 0 815 2169
Size: 815 1354
MscI TGG'CCA
Cuts at: 0 1825 2072 2169
Size: 1825 247 97
Fragments arranged by size:
1825 247 97
MslI CAynn'nnrTG
Cuts at: 0 1207 2169
Size: 1207 962
MspA1I CmG'CkG
Cuts at: 0 276 306 567 834 981 1452 1461 1551
Size: 276 30 261 267 147 471 9 90
Cuts at: 1551 1719 2121 2169
Size: 168 402 48
Fragments arranged by size:
471 402 276 267 261 168 147 90
48 30 9
NaeI GCC'GGC
Cuts at: 0 585 2169
Size: 585 1584
NarI GG'CG_CC
Cuts at: 0 816 2169
Size: 816 1353
NcoI C'CATG_G
Cuts at: 0 551 2169
Size: 551 1618
NgoMIV G'CCGG_C
Cuts at: 0 583 2169
Size: 583 1586
NsiI A_TGCA'T
Cuts at: 0 1403 2169
Size: 1403 766
NspI r_CATG'y
Cuts at: 0 155 2169
Size: 155 2014
PasI CC'CwG_GG
Cuts at: 0 1813 2169
Size: 1813 356
PflMI CCAn_nnn'nTGG
Cuts at: 0 1722 2169
Size: 1722 447
PfoI T'CCnGG_A
Cuts at: 0 1499 1586 1730 1877 2169
Size: 1499 87 144 147 292
Fragments arranged by size:
1499 292 147 144 87
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 742 774 2169
Size: 742 32 1395
Fragments arranged by size:
1395 742 32
PpuMI rG'GwC_Cy
Cuts at: 0 1818 2169
Size: 1818 351
PspOMI G'GGCC_C
Cuts at: 0 709 1526 2169
Size: 709 817 643
Fragments arranged by size:
817 709 643
PspXI vC'TCGA_Gb
Cuts at: 0 802 2169
Size: 802 1367
PstI C_TGCA'G
Cuts at: 0 167 226 974 1031 1343 1856 1998 2169
Size: 167 59 748 57 312 513 142 171
Fragments arranged by size:
748 513 312 171 167 142 59 57
PvuII CAG'CTG
Cuts at: 0 276 306 981 1452 1461 1551 1719 2169
Size: 276 30 675 471 9 90 168 450
Fragments arranged by size:
675 471 450 276 168 90 30 9
SacI G_AGCT'C
Cuts at: 0 122 1586 2169
Size: 122 1464 583
Fragments arranged by size:
1464 583 122
SacII CC_GC'GG
Cuts at: 0 568 2169
Size: 568 1601
SapI GCTCTTCn'nnn_
Cuts at: 0 13 1574 2169
Size: 13 1561 595
Fragments arranged by size:
1561 595 13
SbfI CC_TGCA'GG
Cuts at: 0 1343 2169
Size: 1343 826
ScaI AGT'ACT
Cuts at: 0 652 2169
Size: 652 1517
SfcI C'TryA_G
Cuts at: 0 163 222 970 1027 1339 1852 1994 2169
Size: 163 59 748 57 312 513 142 175
Fragments arranged by size:
748 513 312 175 163 142 59 57
SfoI GGC'GCC
Cuts at: 0 817 2169
Size: 817 1352
SmlI C'TyrA_G
Cuts at: 0 726 802 2169
Size: 726 76 1367
Fragments arranged by size:
1367 726 76
StuI AGG'CCT
Cuts at: 0 1249 2014 2169
Size: 1249 765 155
Fragments arranged by size:
1249 765 155
StyI C'CwwG_G
Cuts at: 0 551 1826 2169
Size: 551 1275 343
Fragments arranged by size:
1275 551 343
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 859 1918 2169
Size: 859 1059 251
Fragments arranged by size:
1059 859 251
TatI w'GTAC_w
Cuts at: 0 650 2169
Size: 650 1519
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 15 47 187 219 727 759 2169
Size: 15 32 140 32 508 32 1410
Fragments arranged by size:
1410 508 140 32 32 32 15
XbaI T'CTAG_A
Cuts at: 0 255 2169
Size: 255 1914
XhoI C'TCGA_G
Cuts at: 0 802 2169
Size: 802 1367
XmnI GAAnn'nnTTC
Cuts at: 0 754 2169
Size: 754 1415
Enzymes that do cut:
AarI AccI AcuI AfeI AhdI AlfI AlwNI ApaI
AvaI BamHI BanI BanII BbeI BbvCI BfrBI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI
Bsp1286I BspEI BspMI BsrBI BsrFI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI CspCI EaeI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I FspI HaeII Hin4I KasI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NsiI
NspI PasI PflMI PfoI PpiI PpuMI PspOMI PspXI
PstI PvuII SacI SacII SapI SbfI ScaI SfcI
SfoI SmlI StuI StyI TaqII TatI TstI XbaI
XhoI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AflII AflIII AgeI AleI AloI
ApaLI ApoI AscI AseI AsiSI AvrII BaeI BbsI
BcgI BciVI BclI BglI BmtI BsaI BsaAI BsaBI
BsiEI BsiWI BsmI BspHI BsrDI BsrGI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI DraI DraIII DrdI EagI
EcoRI EcoRV FalI FseI FspAI HincII HindIII HpaI
KpnI MfeI MluI MmeI NdeI NheI NotI NruI
PacI PciI PmeI PmlI PshAI PsiI PsrI PvuI
RsrII SalI SanDI SexAI SfiI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI SwaI Tth111I XcmI
XmaI ZraI