Restriction map for
pF1KB3304
(Linear) (Six-base) MAPSORT of: KIBB3304.seq Check: 766 from: 1 to: 1143
KIBB3304.orf
With 182 enzymes: SgfI *
February 28, 2007 11:24 ..
AccI GT'mk_AC
Cuts at: 0 311 1143
Size: 311 832
AfeI AGC'GCT
Cuts at: 0 86 303 1143
Size: 86 217 840
Fragments arranged by size:
840 217 86
AflIII A'CryG_T
Cuts at: 0 1068 1143
Size: 1068 75
AlwNI CAG_nnn'CTG
Cuts at: 0 201 640 901 1143
Size: 201 439 261 242
Fragments arranged by size:
439 261 242 201
ApaLI G'TGCA_C
Cuts at: 0 114 1143
Size: 114 1029
ApoI r'AATT_y
Cuts at: 0 742 1143
Size: 742 401
BanII G_rGCy'C
Cuts at: 0 59 1143
Size: 59 1084
BbvCI CC'TCA_GC
Cuts at: 0 305 1143
Size: 305 838
BclI T'GATC_A
Cuts at: 0 531 1143
Size: 531 612
BfrBI ATG'CAT
Cuts at: 0 808 1143
Size: 808 335
Bme1580I G_kGCm'C
Cuts at: 0 118 1143
Size: 118 1025
BmrI ACTGGGnnnn_n'
Cuts at: 0 259 1143
Size: 259 884
Bpu10I CC'TnA_GC
Cuts at: 0 305 1143
Size: 305 838
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 213 1143
Size: 213 930
BsaI GGTCTCn'nnnn_
Cuts at: 0 126 471 1143
Size: 126 345 672
Fragments arranged by size:
672 345 126
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 473 503 1143
Size: 473 30 640
Fragments arranged by size:
640 473 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 43 193 239 242 1143
Size: 43 150 46 3 901
Fragments arranged by size:
901 150 46 43 3
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 127 841 871 1143
Size: 127 714 30 272
Fragments arranged by size:
714 272 127 30
BsiEI CG_ry'CG
Cuts at: 0 681 1143
Size: 681 462
BsiHKAI G_wGCw'C
Cuts at: 0 59 118 1143
Size: 59 59 1025
Fragments arranged by size:
1025 59 59
BsmI GAATG_Cn'
Cuts at: 0 142 1143
Size: 142 1001
BsmBI CGTCTCn'nnnn_
Cuts at: 0 535 1143
Size: 535 608
Bsp1286I G_dGCh'C
Cuts at: 0 59 118 1143
Size: 59 59 1025
Fragments arranged by size:
1025 59 59
BssSI C'ACGA_G
Cuts at: 0 1001 1022 1143
Size: 1001 21 121
Fragments arranged by size:
1001 121 21
BstXI CCAn_nnnn'nTGG
Cuts at: 0 267 1143
Size: 267 876
Bsu36I CC'TnA_GG
Cuts at: 0 148 1143
Size: 148 995
BtgI C'CryG_G
Cuts at: 0 137 1143
Size: 137 1006
BtsI GCAGTG_nn'
Cuts at: 0 116 1143
Size: 116 1027
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 999 1034 1143
Size: 999 35 109
Fragments arranged by size:
999 109 35
EaeI y'GGCC_r
Cuts at: 0 374 1143
Size: 374 769
EarI CTCTTCn'nnn_
Cuts at: 0 923 1143
Size: 923 220
EcoICRI GAG'CTC
Cuts at: 0 57 1143
Size: 57 1086
EcoO109I rG'GnC_Cy
Cuts at: 0 955 1143
Size: 955 188
EcoRI G'AATT_C
Cuts at: 0 742 1143
Size: 742 401
HaeII r_GCGC'y
Cuts at: 0 88 305 1143
Size: 88 217 838
Fragments arranged by size:
838 217 88
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 183 215 234 266 281 313 316 348
Size: 183 32 19 32 15 32 3 32
Cuts at: 348 981 1013 1143
Size: 633 32 130
Fragments arranged by size:
633 183 130 32 32 32 32 32
19 15 3
HincII GTy'rAC
Cuts at: 0 558 1143
Size: 558 585
HpaI GTT'AAC
Cuts at: 0 558 1143
Size: 558 585
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 149 1143
Size: 149 994
MslI CAynn'nnrTG
Cuts at: 0 422 1143
Size: 422 721
MspA1I CmG'CkG
Cuts at: 0 898 1143
Size: 898 245
NcoI C'CATG_G
Cuts at: 0 137 1143
Size: 137 1006
NsiI A_TGCA'T
Cuts at: 0 810 1143
Size: 810 333
PshAI GACnn'nnGTC
Cuts at: 0 547 1143
Size: 547 596
PstI C_TGCA'G
Cuts at: 0 206 1143
Size: 206 937
PvuII CAG'CTG
Cuts at: 0 898 1143
Size: 898 245
SacI G_AGCT'C
Cuts at: 0 59 1143
Size: 59 1084
SfcI C'TryA_G
Cuts at: 0 202 690 1143
Size: 202 488 453
Fragments arranged by size:
488 453 202
SmlI C'TyrA_G
Cuts at: 0 228 1143
Size: 228 915
StyI C'CwwG_G
Cuts at: 0 137 1143
Size: 137 1006
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 273 453 695 905 1067 1143
Size: 273 180 242 210 162 76
Fragments arranged by size:
273 242 210 180 162 76
TatI w'GTAC_w
Cuts at: 0 1115 1143
Size: 1115 28
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 473 505 1143
Size: 473 32 638
Fragments arranged by size:
638 473 32
XmnI GAAnn'nnTTC
Cuts at: 0 1070 1143
Size: 1070 73
Enzymes that do cut:
AccI AfeI AflIII AlwNI ApaLI ApoI BanII BbvCI
BclI BfrBI Bme1580I BmrI Bpu10I BpuEI BsaI BsaXI
BseRI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BssSI
BstXI Bsu36I BtgI BtsI CspCI EaeI EarI EcoICRI
EcoO109I EcoRI HaeII Hin4I HincII HpaI MmeI MslI
MspA1I NcoI NsiI PshAI PstI PvuII SacI SfcI
SmlI StyI TaqII TatI TstI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AflII AgeI AhdI
AleI AlfI AloI ApaI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BanI BbeI BbsI BcgI BciVI
BglI BglII BlpI BmgBI BmtI BplI BpmI BsaAI
BsaBI BsaHI BsaWI BseYI BsiWI BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BstAPI BstBI BstEII
BstYI BstZ17I BtgZI ClaI DraI DraIII DrdI EagI
EciI Eco57MI EcoNI EcoRV FalI FseI FspI FspAI
HindIII KasI KpnI MfeI MluI MscI NaeI NarI
NdeI NgoMIV NheI NotI NruI NspI PacI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PsiI
PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI
Tth111I XbaI XcmI XhoI XmaI ZraI