Restriction map for
pF1KB3312
(Linear) (Six-base) MAPSORT of: KIBB3312.seq Check: 7559 from: 1 to: 2472
KIBB3312.orf
With 182 enzymes: SgfI *
February 28, 2007 11:24 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1862 2472
Size: 1862 610
AfeI AGC'GCT
Cuts at: 0 1982 2472
Size: 1982 490
AflIII A'CryG_T
Cuts at: 0 366 2472
Size: 366 2106
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 235 553 2472
Size: 235 318 1919
Fragments arranged by size:
1919 318 235
AlwNI CAG_nnn'CTG
Cuts at: 0 490 2472
Size: 490 1982
ApoI r'AATT_y
Cuts at: 0 505 645 1692 1762 1948 2116 2274 2467
Size: 505 140 1047 70 186 168 158 193
Cuts at: 2467 2472
Size: 5
Fragments arranged by size:
1047 505 193 186 168 158 140 70
5
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 545 578 2472
Size: 545 33 1894
Fragments arranged by size:
1894 545 33
BamHI G'GATC_C
Cuts at: 0 883 2472
Size: 883 1589
BanII G_rGCy'C
Cuts at: 0 945 1093 2472
Size: 945 148 1379
Fragments arranged by size:
1379 945 148
BbsI GAAGACnn'nnnn_
Cuts at: 0 987 2472
Size: 987 1485
BciVI GTATCCnnnnn_n'
Cuts at: 0 2061 2472
Size: 2061 411
BfrBI ATG'CAT
Cuts at: 0 1909 1968 2472
Size: 1909 59 504
Fragments arranged by size:
1909 504 59
BglII A'GATC_T
Cuts at: 0 1610 2472
Size: 1610 862
BmrI ACTGGGnnnn_n'
Cuts at: 0 748 2472
Size: 748 1724
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1632 2472
Size: 1632 840
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 896 2472
Size: 896 1576
Bpu10I CC'TnA_GC
Cuts at: 0 150 2472
Size: 150 2322
BsaI GGTCTCn'nnnn_
Cuts at: 0 452 2472
Size: 452 2020
BsaBI GATnn'nnATC
Cuts at: 0 2297 2472
Size: 2297 175
BsaWI w'CCGG_w
Cuts at: 0 937 2472
Size: 937 1535
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 2377 2472
Size: 2377 95
Bsp1286I G_dGCh'C
Cuts at: 0 945 1093 2472
Size: 945 148 1379
Fragments arranged by size:
1379 945 148
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 227 2472
Size: 227 2245
BsrBI CCG'CTC
Cuts at: 0 149 2472
Size: 149 2323
BsrDI GCAATG_nn'
Cuts at: 0 214 2472
Size: 214 2258
BstBI TT'CG_AA
Cuts at: 0 1872 2472
Size: 1872 600
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1706 2472
Size: 1706 766
BstYI r'GATC_y
Cuts at: 0 486 883 1610 2298 2472
Size: 486 397 727 688 174
Fragments arranged by size:
727 688 486 397 174
Bsu36I CC'TnA_GG
Cuts at: 0 1079 2472
Size: 1079 1393
BtsI GCAGTG_nn'
Cuts at: 0 1117 2472
Size: 1117 1355
DraI TTT'AAA
Cuts at: 0 431 504 2472
Size: 431 73 1968
Fragments arranged by size:
1968 431 73
DraIII CAC_nnn'GTG
Cuts at: 0 1006 2472
Size: 1006 1466
EaeI y'GGCC_r
Cuts at: 0 972 2472
Size: 972 1500
EarI CTCTTCn'nnn_
Cuts at: 0 1305 2472
Size: 1305 1167
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 896 1862 2472
Size: 896 966 610
Fragments arranged by size:
966 896 610
EcoO109I rG'GnC_Cy
Cuts at: 0 1520 2472
Size: 1520 952
EcoRV GAT'ATC
Cuts at: 0 1989 2472
Size: 1989 483
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 690 1011 2472
Size: 690 321 1461
Fragments arranged by size:
1461 690 321
HaeII r_GCGC'y
Cuts at: 0 1984 2472
Size: 1984 488
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 916 948 2472
Size: 916 32 1524
Fragments arranged by size:
1524 916 32
HindIII A'AGCT_T
Cuts at: 0 250 2472
Size: 250 2222
MfeI C'AATT_G
Cuts at: 0 626 2333 2472
Size: 626 1707 139
Fragments arranged by size:
1707 626 139
MslI CAynn'nnrTG
Cuts at: 0 207 1904 2472
Size: 207 1697 568
Fragments arranged by size:
1697 568 207
MspA1I CmG'CkG
Cuts at: 0 712 2455 2472
Size: 712 1743 17
Fragments arranged by size:
1743 712 17
NsiI A_TGCA'T
Cuts at: 0 1911 1970 2472
Size: 1911 59 502
Fragments arranged by size:
1911 502 59
NspI r_CATG'y
Cuts at: 0 370 1548 2041 2472
Size: 370 1178 493 431
Fragments arranged by size:
1178 493 431 370
PciI A'CATG_T
Cuts at: 0 366 2472
Size: 366 2106
PflMI CCAn_nnn'nTGG
Cuts at: 0 790 844 874 2472
Size: 790 54 30 1598
Fragments arranged by size:
1598 790 54 30
PfoI T'CCnGG_A
Cuts at: 0 873 2472
Size: 873 1599
PsiI TTA'TAA
Cuts at: 0 1904 2472
Size: 1904 568
PstI C_TGCA'G
Cuts at: 0 14 1719 2472
Size: 14 1705 753
Fragments arranged by size:
1705 753 14
PvuII CAG'CTG
Cuts at: 0 712 2455 2472
Size: 712 1743 17
Fragments arranged by size:
1743 712 17
ScaI AGT'ACT
Cuts at: 0 670 2472
Size: 670 1802
SfcI C'TryA_G
Cuts at: 0 10 1715 2472
Size: 10 1705 757
Fragments arranged by size:
1705 757 10
SpeI A'CTAG_T
Cuts at: 0 1147 2472
Size: 1147 1325
SspI AAT'ATT
Cuts at: 0 1458 1562 2472
Size: 1458 104 910
Fragments arranged by size:
1458 910 104
StuI AGG'CCT
Cuts at: 0 1351 1705 2472
Size: 1351 354 767
Fragments arranged by size:
1351 767 354
StyI C'CwwG_G
Cuts at: 0 1166 2472
Size: 1166 1306
SwaI ATTT'AAAT
Cuts at: 0 504 2472
Size: 504 1968
TatI w'GTAC_w
Cuts at: 0 102 196 668 1069 1541 2472
Size: 102 94 472 401 472 931
Fragments arranged by size:
931 472 472 401 102 94
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1193 1225 2472
Size: 1193 32 1247
Fragments arranged by size:
1247 1193 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1489 2472
Size: 1489 983
XmnI GAAnn'nnTTC
Cuts at: 0 1131 1726 2472
Size: 1131 595 746
Fragments arranged by size:
1131 746 595
Enzymes that do cut:
AcuI AfeI AflIII AlfI AlwNI ApoI BaeI BamHI
BanII BbsI BciVI BfrBI BglII BmrI BplI BpmI
Bpu10I BsaI BsaBI BsaWI BseRI Bsp1286I BspMI BsrBI
BsrDI BstBI BstXI BstYI Bsu36I BtsI DraI DraIII
EaeI EarI Eco57MI EcoO109I EcoRV FalI HaeII Hin4I
HindIII MfeI MslI MspA1I NsiI NspI PciI PflMI
PfoI PsiI PstI PvuII ScaI SfcI SpeI SspI
StuI StyI SwaI TatI TstI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AgeI AhdI
AleI AloI ApaI ApaLI AscI AseI AsiSI AvaI
AvrII BanI BbeI BbvCI BcgI BclI BglI BlpI
Bme1580I BmgBI BmtI BpuEI BsaAI BsaHI BsaXI BseYI
BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI BspEI BspHI
BsrFI BsrGI BssHII BssSI BstAPI BstEII BstZ17I BtgI
BtgZI ClaI CspCI DrdI EagI EciI EcoICRI EcoNI
EcoRI FseI FspI FspAI HincII HpaI KasI KpnI
MluI MmeI MscI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI PacI PasI PmeI PmlI PpiI
PpuMI PshAI PspOMI PspXI PsrI PvuI RsrII SacI
SacII SalI SanDI SapI SbfI SexAI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SphI SrfI TaqII
Tth111I XbaI XhoI XmaI ZraI