Restriction map for
pF1KB3313
(Linear) (Six-base) MAPSORT of: KIBB3313.seq Check: 166 from: 1 to: 1659
KIBB3313.orf
With 182 enzymes: SgfI *
April 24, 2009 13:01 ..
AatII G_ACGT'C
Cuts at: 0 298 1659
Size: 298 1361
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 199 416 428 437 974 1659
Size: 199 217 12 9 537 685
Fragments arranged by size:
685 537 217 199 12 9
AflIII A'CryG_T
Cuts at: 0 1076 1659
Size: 1076 583
AhdI GACnn_n'nnGTC
Cuts at: 0 294 1659
Size: 294 1365
AleI CACnn'nnGTG
Cuts at: 0 772 1659
Size: 772 887
AlwNI CAG_nnn'CTG
Cuts at: 0 880 1176 1244 1659
Size: 880 296 68 415
Fragments arranged by size:
880 415 296 68
ApaI G_GGCC'C
Cuts at: 0 933 1030 1659
Size: 933 97 629
Fragments arranged by size:
933 629 97
ApoI r'AATT_y
Cuts at: 0 133 1659
Size: 133 1526
AvrII C'CTAG_G
Cuts at: 0 1278 1659
Size: 1278 381
BamHI G'GATC_C
Cuts at: 0 996 1049 1659
Size: 996 53 610
Fragments arranged by size:
996 610 53
BanII G_rGCy'C
Cuts at: 0 489 933 1030 1659
Size: 489 444 97 629
Fragments arranged by size:
629 489 444 97
BbsI GAAGACnn'nnnn_
Cuts at: 0 434 1270 1659
Size: 434 836 389
Fragments arranged by size:
836 434 389
BlpI GC'TnA_GC
Cuts at: 0 664 1524 1659
Size: 664 860 135
Fragments arranged by size:
860 664 135
Bme1580I G_kGCm'C
Cuts at: 0 933 1030 1659
Size: 933 97 629
Fragments arranged by size:
933 629 97
BmrI ACTGGGnnnn_n'
Cuts at: 0 1025 1659
Size: 1025 634
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 480 1659
Size: 480 1179
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1232 1659
Size: 1232 427
BsaI GGTCTCn'nnnn_
Cuts at: 0 832 1659
Size: 832 827
BsaAI yAC'GTr
Cuts at: 0 1071 1659
Size: 1071 588
BsaBI GATnn'nnATC
Cuts at: 0 638 1312 1659
Size: 638 674 347
Fragments arranged by size:
674 638 347
BsaHI Gr'CG_yC
Cuts at: 0 295 1659
Size: 295 1364
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 358 1659
Size: 358 1301
BseYI C'CCAG_C
Cuts at: 0 1023 1391 1536 1659
Size: 1023 368 145 123
Fragments arranged by size:
1023 368 145 123
BsiEI CG_ry'CG
Cuts at: 0 51 1659
Size: 51 1608
BsmBI CGTCTCn'nnnn_
Cuts at: 0 852 966 1164 1659
Size: 852 114 198 495
Fragments arranged by size:
852 495 198 114
Bsp1286I G_dGCh'C
Cuts at: 0 489 933 1030 1659
Size: 489 444 97 629
Fragments arranged by size:
629 489 444 97
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 976 1659
Size: 976 683
BsrDI GCAATG_nn'
Cuts at: 0 1299 1529 1659
Size: 1299 230 130
Fragments arranged by size:
1299 230 130
BsrGI T'GTAC_A
Cuts at: 0 1589 1659
Size: 1589 70
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1244 1659
Size: 1244 415
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1017 1536 1608 1659
Size: 1017 519 72 51
Fragments arranged by size:
1017 519 72 51
BstYI r'GATC_y
Cuts at: 0 537 639 996 1049 1515 1659
Size: 537 102 357 53 466 144
Fragments arranged by size:
537 466 357 144 102 53
Bsu36I CC'TnA_GG
Cuts at: 0 268 1659
Size: 268 1391
BtgI C'CryG_G
Cuts at: 0 1565 1659
Size: 1565 94
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1270 1659
Size: 1270 389
BtsI GCAGTG_nn'
Cuts at: 0 893 1659
Size: 893 766
DraI TTT'AAA
Cuts at: 0 600 1659
Size: 600 1059
DraIII CAC_nnn'GTG
Cuts at: 0 876 1659
Size: 876 783
DrdI GACnn_nn'nnGTC
Cuts at: 0 1386 1659
Size: 1386 273
EaeI y'GGCC_r
Cuts at: 0 48 64 578 590 1007 1343 1430 1659
Size: 48 16 514 12 417 336 87 229
Fragments arranged by size:
514 417 336 229 87 48 16 12
EagI C'GGCC_G
Cuts at: 0 48 1659
Size: 48 1611
EarI CTCTTCn'nnn_
Cuts at: 0 254 427 456 722 1659
Size: 254 173 29 266 937
Fragments arranged by size:
937 266 254 173 29
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1042 1623 1659
Size: 1042 581 36
Fragments arranged by size:
1042 581 36
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 199 416 428 437 974 1232 1659
Size: 199 217 12 9 537 258 427
Fragments arranged by size:
537 427 258 217 199 12 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 65 1372 1417 1659
Size: 65 1307 45 242
Fragments arranged by size:
1307 242 65 45
EcoO109I rG'GnC_Cy
Cuts at: 0 929 1014 1027 1659
Size: 929 85 13 632
Fragments arranged by size:
929 632 85 13
EcoRV GAT'ATC
Cuts at: 0 55 1659
Size: 55 1604
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 138 1287 1659
Size: 138 1149 372
Fragments arranged by size:
1149 372 138
HaeII r_GCGC'y
Cuts at: 0 1143 1609 1659
Size: 1143 466 50
Fragments arranged by size:
1143 466 50
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 66 98 280 312 1659
Size: 66 32 182 32 1347
Fragments arranged by size:
1347 182 66 32 32
HincII GTy'rAC
Cuts at: 0 1082 1551 1659
Size: 1082 469 108
Fragments arranged by size:
1082 469 108
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 875 974 1582 1659
Size: 875 99 608 77
Fragments arranged by size:
875 608 99 77
MscI TGG'CCA
Cuts at: 0 66 1345 1432 1659
Size: 66 1279 87 227
Fragments arranged by size:
1279 227 87 66
MslI CAynn'nnrTG
Cuts at: 0 772 1659
Size: 772 887
MspA1I CmG'CkG
Cuts at: 0 177 685 760 952 1176 1244 1573 1659
Size: 177 508 75 192 224 68 329 86
Fragments arranged by size:
508 329 224 192 177 86 75 68
NcoI C'CATG_G
Cuts at: 0 1565 1659
Size: 1565 94
NspI r_CATG'y
Cuts at: 0 1080 1659
Size: 1080 579
PasI CC'CwG_GG
Cuts at: 0 1057 1659
Size: 1057 602
PciI A'CATG_T
Cuts at: 0 1076 1659
Size: 1076 583
PflMI CCAn_nnn'nTGG
Cuts at: 0 1257 1659
Size: 1257 402
PfoI T'CCnGG_A
Cuts at: 0 548 920 1442 1659
Size: 548 372 522 217
Fragments arranged by size:
548 522 372 217
PpuMI rG'GwC_Cy
Cuts at: 0 1014 1659
Size: 1014 645
PspOMI G'GGCC_C
Cuts at: 0 929 1026 1659
Size: 929 97 633
Fragments arranged by size:
929 633 97
PstI C_TGCA'G
Cuts at: 0 900 1240 1578 1659
Size: 900 340 338 81
Fragments arranged by size:
900 340 338 81
PvuII CAG'CTG
Cuts at: 0 177 685 760 1176 1244 1573 1659
Size: 177 508 75 416 68 329 86
Fragments arranged by size:
508 416 329 177 86 75 68
RsrII CG'GwC_CG
Cuts at: 0 583 1659
Size: 583 1076
SapI GCTCTTCn'nnn_
Cuts at: 0 456 1659
Size: 456 1203
SexAI A'CCwGG_T
Cuts at: 0 1016 1659
Size: 1016 643
SfcI C'TryA_G
Cuts at: 0 896 1236 1574 1659
Size: 896 340 338 85
Fragments arranged by size:
896 340 338 85
StyI C'CwwG_G
Cuts at: 0 61 822 1001 1010 1278 1329 1565 1601
Size: 61 761 179 9 268 51 236 36
Cuts at: 1601 1659
Size: 58
Fragments arranged by size:
761 268 236 179 61 58 51 36
9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 600 807 1528 1659
Size: 600 207 721 131
Fragments arranged by size:
721 600 207 131
TatI w'GTAC_w
Cuts at: 0 1589 1659
Size: 1589 70
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1438 1470 1659
Size: 1438 32 189
Fragments arranged by size:
1438 189 32
Tth111I GACn'n_nGTC
Cuts at: 0 1099 1659
Size: 1099 560
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1440 1659
Size: 1440 219
XmnI GAAnn'nnTTC
Cuts at: 0 121 1659
Size: 121 1538
ZraI GAC'GTC
Cuts at: 0 296 1659
Size: 296 1363
Enzymes that do cut:
AatII AcuI AflIII AhdI AleI AlwNI ApaI ApoI
AvrII BamHI BanII BbsI BlpI Bme1580I BmrI BplI
BpmI BsaI BsaAI BsaBI BsaHI BseRI BseYI BsiEI
BsmBI Bsp1286I BspMI BsrDI BsrGI BstAPI BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRV FalI
HaeII Hin4I HincII MmeI MscI MslI MspA1I NcoI
NspI PasI PciI PflMI PfoI PpuMI PspOMI PstI
PvuII RsrII SapI SexAI SfcI StyI TaqII TatI
TstI Tth111I XcmI XmnI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AgeI AlfI
AloI ApaLI AscI AseI AsiSI AvaI BaeI BanI
BbeI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BmgBI BmtI Bpu10I BpuEI BsaWI BsaXI BsgI BsiHKAI
BsiWI BsmI BspEI BspHI BsrBI BsrFI BssHII BssSI
BstBI BstEII BstZ17I ClaI CspCI EcoICRI EcoRI FseI
FspI FspAI HindIII HpaI KasI KpnI MfeI MluI
NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI
PacI PmeI PmlI PpiI PshAI PsiI PspXI PsrI
PvuI SacI SacII SalI SanDI SbfI ScaI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI SwaI XbaI XhoI XmaI