Restriction map for
pF1KB3319
(Linear) (Six-base) MAPSORT of: KIBB3319.seq Check: 9709 from: 1 to: 2418
KIBB3319.orf
With 182 enzymes: SgfI *
September 18, 2009 17:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 188 1437 1905 2418
Size: 188 1249 468 513
Fragments arranged by size:
1249 513 468 188
AccI GT'mk_AC
Cuts at: 0 1880 2418
Size: 1880 538
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 403 1120 1744 2418
Size: 403 717 624 674
Fragments arranged by size:
717 674 624 403
AfeI AGC'GCT
Cuts at: 0 1351 1777 2101 2418
Size: 1351 426 324 317
Fragments arranged by size:
1351 426 324 317
AflIII A'CryG_T
Cuts at: 0 62 474 1611 2418
Size: 62 412 1137 807
Fragments arranged by size:
1137 807 412 62
AleI CACnn'nnGTG
Cuts at: 0 2054 2418
Size: 2054 364
AlwNI CAG_nnn'CTG
Cuts at: 0 130 378 1548 2320 2418
Size: 130 248 1170 772 98
Fragments arranged by size:
1170 772 248 130 98
ApaI G_GGCC'C
Cuts at: 0 151 1262 1683 2407 2418
Size: 151 1111 421 724 11
Fragments arranged by size:
1111 724 421 151 11
ApaLI G'TGCA_C
Cuts at: 0 175 2418
Size: 175 2243
AvaI C'yCGr_G
Cuts at: 0 293 2418
Size: 293 2125
BamHI G'GATC_C
Cuts at: 0 1149 2166 2418
Size: 1149 1017 252
Fragments arranged by size:
1149 1017 252
BanI G'GyrC_C
Cuts at: 0 571 742 1888 2056 2418
Size: 571 171 1146 168 362
Fragments arranged by size:
1146 571 362 171 168
BanII G_rGCy'C
Cuts at: 0 151 599 1262 1285 1683 1985 2407 2418
Size: 151 448 663 23 398 302 422 11
Fragments arranged by size:
663 448 422 398 302 151 23 11
BbeI G_GCGC'C
Cuts at: 0 1892 2418
Size: 1892 526
BbsI GAAGACnn'nnnn_
Cuts at: 0 526 2418
Size: 526 1892
BbvCI CC'TCA_GC
Cuts at: 0 2177 2418
Size: 2177 241
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1028 1062 2418
Size: 1028 34 1356
Fragments arranged by size:
1356 1028 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 2250 2418
Size: 2250 168
BglI GCCn_nnn'nGGC
Cuts at: 0 1750 2411 2418
Size: 1750 661 7
Fragments arranged by size:
1750 661 7
BglII A'GATC_T
Cuts at: 0 257 356 2418
Size: 257 99 2062
Fragments arranged by size:
2062 257 99
BlpI GC'TnA_GC
Cuts at: 0 265 1789 2096 2103 2418
Size: 265 1524 307 7 315
Fragments arranged by size:
1524 315 307 265 7
Bme1580I G_kGCm'C
Cuts at: 0 151 179 239 576 747 962 1262 1683
Size: 151 28 60 337 171 215 300 421
Cuts at: 1683 2361 2407 2418
Size: 678 46 11
Fragments arranged by size:
678 421 337 300 215 171 151 60
46 28 11
BmgBI CAC'GTC
Cuts at: 0 63 2418
Size: 63 2355
BmrI ACTGGGnnnn_n'
Cuts at: 0 546 615 786 2418
Size: 546 69 171 1632
Fragments arranged by size:
1632 546 171 69
BmtI G_CTAG'C
Cuts at: 0 863 2418
Size: 863 1555
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1821 2418
Size: 1821 597
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 156 400 571 649 1945 2074 2172 2260
Size: 156 244 171 78 1296 129 98 88
Cuts at: 2260 2287 2353 2418
Size: 27 66 65
Fragments arranged by size:
1296 244 171 156 129 98 88 78
66 65 27
Bpu10I CC'TnA_GC
Cuts at: 0 2177 2224 2418
Size: 2177 47 194
Fragments arranged by size:
2177 194 47
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 339 2418
Size: 339 2079
BsaI GGTCTCn'nnnn_
Cuts at: 0 496 1219 1792 2418
Size: 496 723 573 626
Fragments arranged by size:
723 626 573 496
BsaAI yAC'GTr
Cuts at: 0 213 475 1612 1908 2418
Size: 213 262 1137 296 510
Fragments arranged by size:
1137 510 296 262 213
BsaHI Gr'CG_yC
Cuts at: 0 1271 1889 2418
Size: 1271 618 529
Fragments arranged by size:
1271 618 529
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1120 1150 2418
Size: 1120 30 1268
Fragments arranged by size:
1268 1120 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 153 178 733 862 1141 1225 1360 1750
Size: 153 25 555 129 279 84 135 390
Cuts at: 1750 2380 2418
Size: 630 38
Fragments arranged by size:
630 555 390 279 153 135 129 84
38 25
BseYI C'CCAG_C
Cuts at: 0 955 1548 1671 2418
Size: 955 593 123 747
Fragments arranged by size:
955 747 593 123
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 367 391 853 1072 1315 1540 1567 1930
Size: 367 24 462 219 243 225 27 363
Cuts at: 1930 2418
Size: 488
Fragments arranged by size:
488 462 367 363 243 225 219 27
24
BsiHKAI G_wGCw'C
Cuts at: 0 179 599 1370 1985 2342 2418
Size: 179 420 771 615 357 76
Fragments arranged by size:
771 615 420 357 179 76
Bsp1286I G_dGCh'C
Cuts at: 0 151 179 239 576 599 747 962 1262
Size: 151 28 60 337 23 148 215 300
Cuts at: 1262 1285 1370 1683 1985 2342 2361 2407 2418
Size: 23 85 313 302 357 19 46 11
Fragments arranged by size:
357 337 313 302 300 215 151 148
85 60 46 28 23 23 19 11
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 188 1094 1437 1905 2089 2418
Size: 188 906 343 468 184 329
Fragments arranged by size:
906 468 343 329 188 184
BsrBI CCG'CTC
Cuts at: 0 1493 2418
Size: 1493 925
BsrFI r'CCGG_y
Cuts at: 0 1334 1631 1744 2110 2418
Size: 1334 297 113 366 308
Fragments arranged by size:
1334 366 308 297 113
BssHII G'CGCG_C
Cuts at: 0 44 2069 2071 2418
Size: 44 2025 2 347
Fragments arranged by size:
2025 347 44 2
BssSI C'ACGA_G
Cuts at: 0 1069 1090 2362 2418
Size: 1069 21 1272 56
Fragments arranged by size:
1272 1069 56 21
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2320 2418
Size: 2320 98
BstXI CCAn_nnnn'nTGG
Cuts at: 0 432 924 1050 2418
Size: 432 492 126 1368
Fragments arranged by size:
1368 492 432 126
BstYI r'GATC_y
Cuts at: 0 257 356 1149 1184 2166 2418
Size: 257 99 793 35 982 252
Fragments arranged by size:
982 793 257 252 99 35
Bsu36I CC'TnA_GG
Cuts at: 0 2291 2418
Size: 2291 127
BtgI C'CryG_G
Cuts at: 0 1001 1286 1868 2418
Size: 1001 285 582 550
Fragments arranged by size:
1001 582 550 285
BtsI GCAGTG_nn'
Cuts at: 0 583 854 1495 1606 2418
Size: 583 271 641 111 812
Fragments arranged by size:
812 641 583 271 111
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1743 1778 2418
Size: 1743 35 640
Fragments arranged by size:
1743 640 35
EaeI y'GGCC_r
Cuts at: 0 2 116 942 1391 1697 1751 2039 2418
Size: 2 114 826 449 306 54 288 379
Fragments arranged by size:
826 449 379 306 288 114 54 2
EarI CTCTTCn'nnn_
Cuts at: 0 875 1024 1414 2418
Size: 875 149 390 1004
Fragments arranged by size:
1004 875 390 149
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 682 2418
Size: 682 1736
EcoICRI GAG'CTC
Cuts at: 0 597 1983 2418
Size: 597 1386 435
Fragments arranged by size:
1386 597 435
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 156 400 403 571 649 1120 1744 1945
Size: 156 244 3 168 78 471 624 201
Cuts at: 1945 2074 2172 2260 2287 2353 2418
Size: 129 98 88 27 66 65
Fragments arranged by size:
624 471 244 201 168 156 129 98
88 78 66 65 27 3
EcoNI CCTnn'n_nnAGG
Cuts at: 0 769 1090 2332 2418
Size: 769 321 1242 86
Fragments arranged by size:
1242 769 321 86
EcoO109I rG'GnC_Cy
Cuts at: 0 148 429 909 1095 1258 1679 1680 2282
Size: 148 281 480 186 163 421 1 602
Cuts at: 2282 2295 2404 2418
Size: 13 109 14
Fragments arranged by size:
602 480 421 281 186 163 148 109
14 13 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 543 645 2418
Size: 543 102 1773
Fragments arranged by size:
1773 543 102
FspI TGC'GCA
Cuts at: 0 2008 2418
Size: 2008 410
HaeII r_GCGC'y
Cuts at: 0 1296 1353 1779 1892 1945 2021 2103 2418
Size: 1296 57 426 113 53 76 82 315
Fragments arranged by size:
1296 426 315 113 82 76 57 53
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 2178 2210 2418
Size: 2178 32 208
Fragments arranged by size:
2178 208 32
KasI G'GCGC_C
Cuts at: 0 1888 2418
Size: 1888 530
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1920 2418
Size: 1920 498
MscI TGG'CCA
Cuts at: 0 4 118 1393 1753 2418
Size: 4 114 1275 360 665
Fragments arranged by size:
1275 665 360 114 4
MslI CAynn'nnrTG
Cuts at: 0 539 1849 2054 2418
Size: 539 1310 205 364
Fragments arranged by size:
1310 539 364 205
MspA1I CmG'CkG
Cuts at: 0 201 308 398 1329 1548 1793 2134 2202
Size: 201 107 90 931 219 245 341 68
Cuts at: 2202 2418
Size: 216
Fragments arranged by size:
931 341 245 219 216 201 107 90
68
NaeI GCC'GGC
Cuts at: 0 1746 2112 2418
Size: 1746 366 306
Fragments arranged by size:
1746 366 306
NarI GG'CG_CC
Cuts at: 0 1889 2418
Size: 1889 529
NcoI C'CATG_G
Cuts at: 0 1001 2418
Size: 1001 1417
NgoMIV G'CCGG_C
Cuts at: 0 1744 2110 2418
Size: 1744 366 308
Fragments arranged by size:
1744 366 308
NheI G'CTAG_C
Cuts at: 0 859 2418
Size: 859 1559
NspI r_CATG'y
Cuts at: 0 1080 2418
Size: 1080 1338
PasI CC'CwG_GG
Cuts at: 0 2142 2400 2418
Size: 2142 258 18
Fragments arranged by size:
2142 258 18
PflMI CCAn_nnn'nTGG
Cuts at: 0 201 498 1548 2418
Size: 201 297 1050 870
Fragments arranged by size:
1050 870 297 201
PfoI T'CCnGG_A
Cuts at: 0 1625 2418
Size: 1625 793
PmlI CAC'GTG
Cuts at: 0 475 1612 1908 2418
Size: 475 1137 296 510
Fragments arranged by size:
1137 510 475 296
PpuMI rG'GwC_Cy
Cuts at: 0 429 909 1095 2295 2418
Size: 429 480 186 1200 123
Fragments arranged by size:
1200 480 429 186 123
PspOMI G'GGCC_C
Cuts at: 0 147 1258 1679 2403 2418
Size: 147 1111 421 724 15
Fragments arranged by size:
1111 724 421 147 15
PstI C_TGCA'G
Cuts at: 0 1601 2119 2418
Size: 1601 518 299
Fragments arranged by size:
1601 518 299
PvuII CAG'CTG
Cuts at: 0 201 308 1329 1548 2134 2202 2418
Size: 201 107 1021 219 586 68 216
Fragments arranged by size:
1021 586 219 216 201 107 68
SacI G_AGCT'C
Cuts at: 0 599 1985 2418
Size: 599 1386 433
Fragments arranged by size:
1386 599 433
SapI GCTCTTCn'nnn_
Cuts at: 0 875 2418
Size: 875 1543
SfcI C'TryA_G
Cuts at: 0 1597 2115 2418
Size: 1597 518 303
Fragments arranged by size:
1597 518 303
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 2411 2418
Size: 2411 7
SfoI GGC'GCC
Cuts at: 0 1890 2418
Size: 1890 528
SgrAI Cr'CCGG_yG
Cuts at: 0 1334 2418
Size: 1334 1084
SmlI C'TyrA_G
Cuts at: 0 318 2418
Size: 318 2100
SphI G_CATG'C
Cuts at: 0 1080 2418
Size: 1080 1338
StyI C'CwwG_G
Cuts at: 0 56 866 890 1001 1511 1554 2409 2418
Size: 56 810 24 111 510 43 855 9
Fragments arranged by size:
855 810 510 111 56 43 24 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 730 2418
Size: 730 1688
TatI w'GTAC_w
Cuts at: 0 167 1136 2418
Size: 167 969 1282
Fragments arranged by size:
1282 969 167
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 726 758 1418 1450 2418
Size: 726 32 660 32 968
Fragments arranged by size:
968 726 660 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 229 778 2418
Size: 229 549 1640
Fragments arranged by size:
1640 549 229
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 683 2418
Size: 683 1735
XmnI GAAnn'nnTTC
Cuts at: 0 628 2418
Size: 628 1790
Enzymes that do cut:
AarI AccI AcuI AfeI AflIII AleI AlwNI ApaI
ApaLI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI
BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI BsrBI
BsrFI BssHII BssSI BstAPI BstXI BstYI Bsu36I BtgI
BtsI CspCI EaeI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I FalI FspI HaeII Hin4I KasI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NheI NspI
PasI PflMI PfoI PmlI PpuMI PspOMI PstI PvuII
SacI SapI SfcI SfiI SfoI SgrAI SmlI SphI
StyI TaqII TatI TstI Tth111I XcmI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AflII AgeI AhdI AlfI AloI
ApoI AscI AseI AsiSI AvrII BaeI BclI BfrBI
BsaBI BsaWI BsiEI BsiWI BsmI BsmBI BspEI BspHI
BsrDI BsrGI BstBI BstEII BstZ17I BtgZI ClaI DraI
DraIII DrdI EagI EcoRI EcoRV FseI FspAI HincII
HindIII HpaI KpnI MfeI MluI NdeI NotI NruI
NsiI PacI PciI PmeI PpiI PshAI PsiI PspXI
PsrI PvuI RsrII SacII SalI SanDI SbfI ScaI
SexAI SgfI SmaI SnaBI SpeI SrfI SspI StuI
SwaI XbaI XhoI XmaI ZraI