Restriction map for
pF1KB3331
(Linear) (Six-base) MAPSORT of: KIBB3331.seq Check: 8598 from: 1 to: 1542
KIBB3331.orf
With 182 enzymes: SgfI *
April 24, 2009 13:01 ..
AccI GT'mk_AC
Cuts at: 0 1463 1542
Size: 1463 79
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1460 1542
Size: 1460 82
AflIII A'CryG_T
Cuts at: 0 68 1542
Size: 68 1474
AleI CACnn'nnGTG
Cuts at: 0 37 1542
Size: 37 1505
AlwNI CAG_nnn'CTG
Cuts at: 0 310 1542
Size: 310 1232
ApoI r'AATT_y
Cuts at: 0 486 1195 1263 1542
Size: 486 709 68 279
Fragments arranged by size:
709 486 279 68
BanI G'GyrC_C
Cuts at: 0 569 1300 1542
Size: 569 731 242
Fragments arranged by size:
731 569 242
BanII G_rGCy'C
Cuts at: 0 775 1542
Size: 775 767
BbsI GAAGACnn'nnnn_
Cuts at: 0 803 1542
Size: 803 739
BclI T'GATC_A
Cuts at: 0 1410 1542
Size: 1410 132
BfrBI ATG'CAT
Cuts at: 0 1308 1542
Size: 1308 234
BglII A'GATC_T
Cuts at: 0 686 1542
Size: 686 856
BmrI ACTGGGnnnn_n'
Cuts at: 0 558 737 1542
Size: 558 179 805
Fragments arranged by size:
805 558 179
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1157 1307 1341 1542
Size: 1157 150 34 201
Fragments arranged by size:
1157 201 150 34
Bpu10I CC'TnA_GC
Cuts at: 0 375 1542
Size: 375 1167
BsaBI GATnn'nnATC
Cuts at: 0 413 1542
Size: 413 1129
BsaWI w'CCGG_w
Cuts at: 0 1228 1542
Size: 1228 314
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1265 1295 1542
Size: 1265 30 247
Fragments arranged by size:
1265 247 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 22 1542
Size: 22 1520
BseYI C'CCAG_C
Cuts at: 0 614 815 1542
Size: 614 201 727
Fragments arranged by size:
727 614 201
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 350 509 887 917 1542
Size: 350 159 378 30 625
Fragments arranged by size:
625 378 350 159 30
BsmI GAATG_Cn'
Cuts at: 0 219 353 1542
Size: 219 134 1189
Fragments arranged by size:
1189 219 134
Bsp1286I G_dGCh'C
Cuts at: 0 775 1542
Size: 775 767
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 274 1542
Size: 274 1268
BsrGI T'GTAC_A
Cuts at: 0 27 966 1542
Size: 27 939 576
Fragments arranged by size:
939 576 27
BstEII G'GTnAC_C
Cuts at: 0 909 1542
Size: 909 633
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1508 1542
Size: 1508 34
BstYI r'GATC_y
Cuts at: 0 338 664 686 819 1071 1542
Size: 338 326 22 133 252 471
Fragments arranged by size:
471 338 326 252 133 22
BstZ17I GTA'TAC
Cuts at: 0 1464 1542
Size: 1464 78
BtgI C'CryG_G
Cuts at: 0 577 1542
Size: 577 965
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 496 1542
Size: 496 1046
BtsI GCAGTG_nn'
Cuts at: 0 875 1195 1542
Size: 875 320 347
Fragments arranged by size:
875 347 320
ClaI AT'CG_AT
Cuts at: 0 899 1542
Size: 899 643
EarI CTCTTCn'nnn_
Cuts at: 0 49 104 1542
Size: 49 55 1438
Fragments arranged by size:
1438 55 49
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1157 1307 1341 1460 1542
Size: 1157 150 34 119 82
Fragments arranged by size:
1157 150 119 82 34
EcoO109I rG'GnC_Cy
Cuts at: 0 59 205 307 1542
Size: 59 146 102 1235
Fragments arranged by size:
1235 146 102 59
EcoRI G'AATT_C
Cuts at: 0 486 1542
Size: 486 1056
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 208 1542
Size: 208 1334
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 762 794 1369 1401 1542
Size: 762 32 575 32 141
Fragments arranged by size:
762 575 141 32 32
HindIII A'AGCT_T
Cuts at: 0 139 1542
Size: 139 1403
MfeI C'AATT_G
Cuts at: 0 491 1542
Size: 491 1051
MslI CAynn'nnrTG
Cuts at: 0 37 851 863 989 1542
Size: 37 814 12 126 553
Fragments arranged by size:
814 553 126 37 12
MspA1I CmG'CkG
Cuts at: 0 364 1357 1438 1508 1542
Size: 364 993 81 70 34
Fragments arranged by size:
993 364 81 70 34
NdeI CA'TA_TG
Cuts at: 0 170 228 1542
Size: 170 58 1314
Fragments arranged by size:
1314 170 58
NsiI A_TGCA'T
Cuts at: 0 1310 1542
Size: 1310 232
NspI r_CATG'y
Cuts at: 0 72 1542
Size: 72 1470
PciI A'CATG_T
Cuts at: 0 68 1542
Size: 68 1474
PflMI CCAn_nnn'nTGG
Cuts at: 0 310 1542
Size: 310 1232
PpuMI rG'GwC_Cy
Cuts at: 0 59 205 307 1542
Size: 59 146 102 1235
Fragments arranged by size:
1235 146 102 59
PstI C_TGCA'G
Cuts at: 0 1023 1542
Size: 1023 519
PvuII CAG'CTG
Cuts at: 0 364 1357 1438 1508 1542
Size: 364 993 81 70 34
Fragments arranged by size:
993 364 81 70 34
ScaI AGT'ACT
Cuts at: 0 741 1542
Size: 741 801
SfcI C'TryA_G
Cuts at: 0 476 588 915 1001 1019 1542
Size: 476 112 327 86 18 523
Fragments arranged by size:
523 476 327 112 86 18
SspI AAT'ATT
Cuts at: 0 1394 1542
Size: 1394 148
StyI C'CwwG_G
Cuts at: 0 401 1542
Size: 401 1141
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 587 1542
Size: 587 955
TatI w'GTAC_w
Cuts at: 0 27 739 966 1542
Size: 27 712 227 576
Fragments arranged by size:
712 576 227 27
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 80 112 1542
Size: 80 32 1430
Fragments arranged by size:
1430 80 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 563 1542
Size: 563 979
Enzymes that do cut:
AccI AcuI AflIII AleI AlwNI ApoI BanI BanII
BbsI BclI BfrBI BglII BmrI BpmI Bpu10I BsaBI
BsaWI BsaXI BseRI BseYI BsgI BsmI Bsp1286I BspMI
BsrGI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI
ClaI EarI Eco57MI EcoO109I EcoRI FalI Hin4I HindIII
MfeI MslI MspA1I NdeI NsiI NspI PciI PflMI
PpuMI PstI PvuII ScaI SfcI SspI StyI TaqII
TatI TstI XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AlfI AloI ApaI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BbeI BbvCI BcgI BciVI BglI
BlpI Bme1580I BmgBI BmtI BplI BpuEI BsaI BsaAI
BsaHI BsiEI BsiHKAI BsiWI BsmBI BspEI BspHI BsrBI
BsrDI BsrFI BssHII BssSI BstAPI BstBI Bsu36I CspCI
DraI DraIII DrdI EaeI EagI EciI EcoICRI EcoNI
EcoRV FseI FspI FspAI HaeII HincII HpaI KasI
KpnI MluI MmeI MscI NaeI NarI NcoI NgoMIV
NheI NotI NruI PacI PasI PfoI PmeI PmlI
PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII
SacI SacII SalI SanDI SapI SbfI SexAI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI StuI SwaI Tth111I XbaI XhoI XmaI XmnI
ZraI