Restriction map for
pF1KB3336
(Linear) (Six-base) MAPSORT of: KIBB3336.seq Check: 3120 from: 1 to: 1653
KIBB3336.orf
With 182 enzymes: SgfI *
March 3, 2008 10:56 ..
AatII G_ACGT'C
Cuts at: 0 899 1653
Size: 899 754
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 669 841 853 921 1653
Size: 669 172 12 68 732
Fragments arranged by size:
732 669 172 68 12
AflII C'TTAA_G
Cuts at: 0 1044 1653
Size: 1044 609
AlwNI CAG_nnn'CTG
Cuts at: 0 291 678 1230 1397 1413 1653
Size: 291 387 552 167 16 240
Fragments arranged by size:
552 387 291 240 167 16
ApaI G_GGCC'C
Cuts at: 0 1572 1653
Size: 1572 81
AvaI C'yCGr_G
Cuts at: 0 117 1653
Size: 117 1536
BanI G'GyrC_C
Cuts at: 0 954 1026 1194 1653
Size: 954 72 168 459
Fragments arranged by size:
954 459 168 72
BanII G_rGCy'C
Cuts at: 0 159 276 664 880 1037 1572 1653
Size: 159 117 388 216 157 535 81
Fragments arranged by size:
535 388 216 159 157 117 81
BbsI GAAGACnn'nnnn_
Cuts at: 0 1175 1199 1653
Size: 1175 24 454
Fragments arranged by size:
1175 454 24
BbvCI CC'TCA_GC
Cuts at: 0 751 1653
Size: 751 902
BclI T'GATC_A
Cuts at: 0 791 1653
Size: 791 862
BglI GCCn_nnn'nGGC
Cuts at: 0 947 1416 1551 1653
Size: 947 469 135 102
Fragments arranged by size:
947 469 135 102
BglII A'GATC_T
Cuts at: 0 962 1653
Size: 962 691
BlpI GC'TnA_GC
Cuts at: 0 874 1653
Size: 874 779
Bme1580I G_kGCm'C
Cuts at: 0 1197 1572 1653
Size: 1197 375 81
Fragments arranged by size:
1197 375 81
BmrI ACTGGGnnnn_n'
Cuts at: 0 90 600 1289 1407 1653
Size: 90 510 689 118 246
Fragments arranged by size:
689 510 246 118 90
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 162 514 604 678 687 1003 1251 1609
Size: 162 352 90 74 9 316 248 358
Cuts at: 1609 1653
Size: 44
Fragments arranged by size:
358 352 316 248 162 90 74 44
9
Bpu10I CC'TnA_GC
Cuts at: 0 751 1031 1466 1653
Size: 751 280 435 187
Fragments arranged by size:
751 435 280 187
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 541 1653
Size: 541 1112
BsaI GGTCTCn'nnnn_
Cuts at: 0 424 1653
Size: 424 1229
BsaAI yAC'GTr
Cuts at: 0 426 1653
Size: 426 1227
BsaBI GATnn'nnATC
Cuts at: 0 358 1653
Size: 358 1295
BsaHI Gr'CG_yC
Cuts at: 0 24 896 1247 1653
Size: 24 872 351 406
Fragments arranged by size:
872 406 351 24
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 881 911 1653
Size: 881 30 742
Fragments arranged by size:
881 742 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 25 34 550 730 905 1291 1507 1653
Size: 25 9 516 180 175 386 216 146
Fragments arranged by size:
516 386 216 180 175 146 25 9
BseYI C'CCAG_C
Cuts at: 0 452 869 1312 1406 1486 1653
Size: 452 417 443 94 80 167
Fragments arranged by size:
452 443 417 167 94 80
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1239 1653
Size: 1239 414
BsiHKAI G_wGCw'C
Cuts at: 0 880 1653
Size: 880 773
BsmBI CGTCTCn'nnnn_
Cuts at: 0 580 887 1653
Size: 580 307 766
Fragments arranged by size:
766 580 307
Bsp1286I G_dGCh'C
Cuts at: 0 159 276 664 880 1037 1197 1572 1653
Size: 159 117 388 216 157 160 375 81
Fragments arranged by size:
388 375 216 160 159 157 117 81
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1286 1653
Size: 1286 367
BsrFI r'CCGG_y
Cuts at: 0 1348 1653
Size: 1348 305
BssSI C'ACGA_G
Cuts at: 0 742 1653
Size: 742 911
BstYI r'GATC_y
Cuts at: 0 575 962 1653
Size: 575 387 691
Fragments arranged by size:
691 575 387
Bsu36I CC'TnA_GG
Cuts at: 0 316 1653
Size: 316 1337
BtgI C'CryG_G
Cuts at: 0 724 814 1653
Size: 724 90 839
Fragments arranged by size:
839 724 90
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 357 1653
Size: 357 1296
BtsI GCAGTG_nn'
Cuts at: 0 1325 1347 1653
Size: 1325 22 306
Fragments arranged by size:
1325 306 22
DraIII CAC_nnn'GTG
Cuts at: 0 1650 1653
Size: 1650 3
EaeI y'GGCC_r
Cuts at: 0 190 356 732 1187 1653
Size: 190 166 376 455 466
Fragments arranged by size:
466 455 376 190 166
EarI CTCTTCn'nnn_
Cuts at: 0 918 1291 1349 1653
Size: 918 373 58 304
Fragments arranged by size:
918 373 304 58
EcoICRI GAG'CTC
Cuts at: 0 878 1653
Size: 878 775
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 162 514 604 669 678 687 841 853
Size: 162 352 90 65 9 9 154 12
Cuts at: 853 921 1003 1251 1609 1653
Size: 68 82 248 358 44
Fragments arranged by size:
358 352 248 162 154 90 82 68
65 44 12 9 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 638 709 1015 1232 1539 1653
Size: 638 71 306 217 307 114
Fragments arranged by size:
638 307 306 217 114 71
EcoO109I rG'GnC_Cy
Cuts at: 0 540 828 1401 1417 1497 1568 1569 1635
Size: 540 288 573 16 80 71 1 66
Cuts at: 1635 1653
Size: 18
Fragments arranged by size:
573 540 288 80 71 66 18 16
1
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 113 145 881 913 1024 1056 1144 1176
Size: 113 32 736 32 111 32 88 32
Cuts at: 1176 1653
Size: 477
Fragments arranged by size:
736 477 113 111 88 32 32 32
32
HincII GTy'rAC
Cuts at: 0 303 1653
Size: 303 1350
MscI TGG'CCA
Cuts at: 0 358 1653
Size: 358 1295
MslI CAynn'nnrTG
Cuts at: 0 778 1653
Size: 778 875
MspA1I CmG'CkG
Cuts at: 0 79 755 873 1005 1070 1653
Size: 79 676 118 132 65 583
Fragments arranged by size:
676 583 132 118 79 65
NcoI C'CATG_G
Cuts at: 0 814 1653
Size: 814 839
NspI r_CATG'y
Cuts at: 0 107 1653
Size: 107 1546
PasI CC'CwG_GG
Cuts at: 0 1333 1413 1514 1653
Size: 1333 80 101 139
Fragments arranged by size:
1333 139 101 80
PflMI CCAn_nnn'nTGG
Cuts at: 0 678 725 1397 1413 1653
Size: 678 47 672 16 240
Fragments arranged by size:
678 672 240 47 16
PfoI T'CCnGG_A
Cuts at: 0 362 1106 1653
Size: 362 744 547
Fragments arranged by size:
744 547 362
PmlI CAC'GTG
Cuts at: 0 426 1653
Size: 426 1227
PpuMI rG'GwC_Cy
Cuts at: 0 828 1401 1497 1635 1653
Size: 828 573 96 138 18
Fragments arranged by size:
828 573 138 96 18
PspOMI G'GGCC_C
Cuts at: 0 1568 1653
Size: 1568 85
PstI C_TGCA'G
Cuts at: 0 78 164 702 802 1016 1226 1653
Size: 78 86 538 100 214 210 427
Fragments arranged by size:
538 427 214 210 100 86 78
PvuII CAG'CTG
Cuts at: 0 873 1005 1070 1653
Size: 873 132 65 583
Fragments arranged by size:
873 583 132 65
SacI G_AGCT'C
Cuts at: 0 880 1653
Size: 880 773
SanDI GG'GwC_CC
Cuts at: 0 1401 1653
Size: 1401 252
SapI GCTCTTCn'nnn_
Cuts at: 0 1291 1653
Size: 1291 362
SexAI A'CCwGG_T
Cuts at: 0 1442 1653
Size: 1442 211
SfcI C'TryA_G
Cuts at: 0 74 160 698 798 1012 1222 1653
Size: 74 86 538 100 214 210 431
Fragments arranged by size:
538 431 214 210 100 86 74
SmlI C'TyrA_G
Cuts at: 0 117 556 1044 1653
Size: 117 439 488 609
Fragments arranged by size:
609 488 439 117
StuI AGG'CCT
Cuts at: 0 1129 1545 1653
Size: 1129 416 108
Fragments arranged by size:
1129 416 108
StyI C'CwwG_G
Cuts at: 0 705 814 1115 1653
Size: 705 109 301 538
Fragments arranged by size:
705 538 301 109
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 619 1212 1653
Size: 619 593 441
Fragments arranged by size:
619 593 441
TatI w'GTAC_w
Cuts at: 0 782 1139 1653
Size: 782 357 514
Fragments arranged by size:
782 514 357
Tth111I GACn'n_nGTC
Cuts at: 0 307 513 1653
Size: 307 206 1140
Fragments arranged by size:
1140 307 206
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 527 1653
Size: 527 1126
XhoI C'TCGA_G
Cuts at: 0 117 1653
Size: 117 1536
ZraI GAC'GTC
Cuts at: 0 897 1653
Size: 897 756
Enzymes that do cut:
AatII AcuI AflII AlwNI ApaI AvaI BanI BanII
BbsI BbvCI BclI BglI BglII BlpI Bme1580I BmrI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaXI
BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspMI BsrFI
BssSI BstYI Bsu36I BtgI BtgZI BtsI DraIII EaeI
EarI EcoICRI Eco57MI EcoNI EcoO109I Hin4I HincII MscI
MslI MspA1I NcoI NspI PasI PflMI PfoI PmlI
PpuMI PspOMI PstI PvuII SacI SanDI SapI SexAI
SfcI SmlI StuI StyI TaqII TatI Tth111I XcmI
XhoI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflIII AgeI AhdI
AleI AlfI AloI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbeI BcgI BciVI BfrBI BmgBI
BmtI BplI BsaWI BsiEI BsiWI BsmI BspEI BspHI
BsrBI BsrDI BsrGI BssHII BstAPI BstBI BstEII BstXI
BstZ17I ClaI CspCI DraI DrdI EagI EciI EcoRI
EcoRV FalI FseI FspI FspAI HaeII HindIII HpaI
KasI KpnI MfeI MluI MmeI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI PacI PciI PmeI
PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacII
SalI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI SwaI TstI XbaI
XmaI XmnI