Restriction map for
pF1KB3343
(Linear) (Six-base) MAPSORT of: KIBB3343.seq Check: 9880 from: 1 to: 2691
KIBB3343.orf
With 182 enzymes: SgfI *
September 18, 2009 17:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 125 139 1896 2691
Size: 125 14 1757 795
Fragments arranged by size:
1757 795 125 14
AatII G_ACGT'C
Cuts at: 0 675 2691
Size: 675 2016
AccI GT'mk_AC
Cuts at: 0 521 2691
Size: 521 2170
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 517 2650 2691
Size: 517 2133 41
Fragments arranged by size:
2133 517 41
AhdI GACnn_n'nnGTC
Cuts at: 0 1308 2646 2691
Size: 1308 1338 45
Fragments arranged by size:
1338 1308 45
AleI CACnn'nnGTG
Cuts at: 0 622 2691
Size: 622 2069
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1210 2691
Size: 1210 1481
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1555 1587 2691
Size: 1555 32 1104
Fragments arranged by size:
1555 1104 32
AlwNI CAG_nnn'CTG
Cuts at: 0 18 96 741 1223 2272 2691
Size: 18 78 645 482 1049 419
Fragments arranged by size:
1049 645 482 419 78 18
ApaI G_GGCC'C
Cuts at: 0 1775 2489 2691
Size: 1775 714 202
Fragments arranged by size:
1775 714 202
AvaI C'yCGr_G
Cuts at: 0 629 2428 2523 2691
Size: 629 1799 95 168
Fragments arranged by size:
1799 629 168 95
BanI G'GyrC_C
Cuts at: 0 358 908 2601 2691
Size: 358 550 1693 90
Fragments arranged by size:
1693 550 358 90
BanII G_rGCy'C
Cuts at: 0 407 691 890 1003 1211 1775 1832 2095
Size: 407 284 199 113 208 564 57 263
Cuts at: 2095 2265 2306 2489 2691
Size: 170 41 183 202
Fragments arranged by size:
564 407 284 263 208 202 199 183
170 113 57 41
BbvCI CC'TCA_GC
Cuts at: 0 877 2691
Size: 877 1814
BglI GCCn_nnn'nGGC
Cuts at: 0 37 2691
Size: 37 2654
BglII A'GATC_T
Cuts at: 0 1677 2691
Size: 1677 1014
BlpI GC'TnA_GC
Cuts at: 0 772 2691
Size: 772 1919
Bme1580I G_kGCm'C
Cuts at: 0 278 361 932 1775 2489 2597 2606 2691
Size: 278 83 571 843 714 108 9 85
Fragments arranged by size:
843 714 571 278 108 85 83 9
BmrI ACTGGGnnnn_n'
Cuts at: 0 865 1233 2691
Size: 865 368 1458
Fragments arranged by size:
1458 865 368
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1311 2691
Size: 1311 1380
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 229 474 526 763 1156 1225 1293 1582
Size: 229 245 52 237 393 69 68 289
Cuts at: 1582 1649 1850 1897 2023 2135 2279 2691
Size: 67 201 47 126 112 144 412
Fragments arranged by size:
412 393 289 245 237 229 201 144
126 112 69 68 67 52 47
Bpu10I CC'TnA_GC
Cuts at: 0 401 877 1556 2691
Size: 401 476 679 1135
Fragments arranged by size:
1135 679 476 401
BsaI GGTCTCn'nnnn_
Cuts at: 0 819 1159 2691
Size: 819 340 1532
Fragments arranged by size:
1532 819 340
BsaAI yAC'GTr
Cuts at: 0 624 1145 2691
Size: 624 521 1546
Fragments arranged by size:
1546 624 521
BsaHI Gr'CG_yC
Cuts at: 0 672 2691
Size: 672 2019
BsaWI w'CCGG_w
Cuts at: 0 2504 2691
Size: 2504 187
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 202 232 847 877 1555 1585 1783 1813
Size: 202 30 615 30 678 30 198 30
Cuts at: 1813 1870 1900 2691
Size: 57 30 791
Fragments arranged by size:
791 678 615 202 198 57 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 553 1438 2127 2691
Size: 553 885 689 564
Fragments arranged by size:
885 689 564 553
BseYI C'CCAG_C
Cuts at: 0 47 158 383 610 676 701 710 728
Size: 47 111 225 227 66 25 9 18
Cuts at: 728 838 1070 1441 1699 1706 1897 2224 2691
Size: 110 232 371 258 7 191 327 467
Fragments arranged by size:
467 371 327 258 232 227 225 191
111 110 66 47 25 18 9 7
BsiHKAI G_wGCw'C
Cuts at: 0 602 773 868 1003 1370 2095 2306 2691
Size: 602 171 95 135 367 725 211 385
Fragments arranged by size:
725 602 385 367 211 171 135 95
BsmBI CGTCTCn'nnnn_
Cuts at: 0 966 2691
Size: 966 1725
Bsp1286I G_dGCh'C
Cuts at: 0 278 361 407 602 691 773 868 890
Size: 278 83 46 195 89 82 95 22
Cuts at: 890 932 1003 1211 1370 1775 1832 2095 2265
Size: 42 71 208 159 405 57 263 170
Cuts at: 2265 2306 2489 2597 2606 2691
Size: 41 183 108 9 85
Fragments arranged by size:
405 278 263 208 195 183 170 159
108 95 89 85 83 82 71 57
46 42 41 22 9
BspEI T'CCGG_A
Cuts at: 0 2504 2691
Size: 2504 187
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 125 139 422 1896 2691
Size: 125 14 283 1474 795
Fragments arranged by size:
1474 795 283 125 14
BsrBI CCG'CTC
Cuts at: 0 1799 2691
Size: 1799 892
BsrFI r'CCGG_y
Cuts at: 0 1255 2321 2691
Size: 1255 1066 370
Fragments arranged by size:
1255 1066 370
BssHII G'CGCG_C
Cuts at: 0 1291 2691
Size: 1291 1400
BstAPI GCAn_nnn'nTGC
Cuts at: 0 96 606 2691
Size: 96 510 2085
Fragments arranged by size:
2085 510 96
BstEII G'GTnAC_C
Cuts at: 0 2333 2691
Size: 2333 358
BstYI r'GATC_y
Cuts at: 0 501 1118 1268 1430 1677 2691
Size: 501 617 150 162 247 1014
Fragments arranged by size:
1014 617 501 247 162 150
Bsu36I CC'TnA_GG
Cuts at: 0 2360 2691
Size: 2360 331
BtgI C'CryG_G
Cuts at: 0 26 945 1537 1952 2691
Size: 26 919 592 415 739
Fragments arranged by size:
919 739 592 415 26
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1344 1948 2691
Size: 1344 604 743
Fragments arranged by size:
1344 743 604
BtsI GCAGTG_nn'
Cuts at: 0 912 2691
Size: 912 1779
EaeI y'GGCC_r
Cuts at: 0 803 948 1139 2243 2691
Size: 803 145 191 1104 448
Fragments arranged by size:
1104 803 448 191 145
EarI CTCTTCn'nnn_
Cuts at: 0 854 2623 2691
Size: 854 1769 68
Fragments arranged by size:
1769 854 68
EcoICRI GAG'CTC
Cuts at: 0 1001 2093 2304 2691
Size: 1001 1092 211 387
Fragments arranged by size:
1092 1001 387 211
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 229 474 517 526 763 1156 1225 1293
Size: 229 245 43 9 237 393 69 68
Cuts at: 1293 1582 1649 1850 1897 2023 2135 2279 2650
Size: 289 67 201 47 126 112 144 371
Cuts at: 2650 2691
Size: 41
Fragments arranged by size:
393 371 289 245 237 229 201 144
126 112 69 68 67 47 43 41
9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1215 2554 2691
Size: 1215 1339 137
Fragments arranged by size:
1339 1215 137
EcoO109I rG'GnC_Cy
Cuts at: 0 201 411 1244 1620 1772 1871 2017 2109
Size: 201 210 833 376 152 99 146 92
Cuts at: 2109 2171 2269 2433 2485 2527 2691
Size: 62 98 164 52 42 164
Fragments arranged by size:
833 376 210 201 164 164 152 146
99 98 92 62 52 42
FspI TGC'GCA
Cuts at: 0 1411 2691
Size: 1411 1280
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 202 234 316 348 1008 1040 1294 1326
Size: 202 32 82 32 660 32 254 32
Cuts at: 1326 1630 1662 2308 2340 2632 2664 2691
Size: 304 32 646 32 292 32 27
Fragments arranged by size:
660 646 304 292 254 202 82 32
32 32 32 32 32 32 27
HincII GTy'rAC
Cuts at: 0 171 324 2670 2691
Size: 171 153 2346 21
Fragments arranged by size:
2346 171 153 21
MfeI C'AATT_G
Cuts at: 0 2312 2691
Size: 2312 379
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 363 593 2691
Size: 363 230 2098
Fragments arranged by size:
2098 363 230
MscI TGG'CCA
Cuts at: 0 805 2245 2691
Size: 805 1440 446
Fragments arranged by size:
1440 805 446
MslI CAynn'nnrTG
Cuts at: 0 622 1015 1363 1634 2691
Size: 622 393 348 271 1057
Fragments arranged by size:
1057 622 393 348 271
MspA1I CmG'CkG
Cuts at: 0 87 162 269 776 1669 2691
Size: 87 75 107 507 893 1022
Fragments arranged by size:
1022 893 507 107 87 75
NcoI C'CATG_G
Cuts at: 0 26 2691
Size: 26 2665
NspI r_CATG'y
Cuts at: 0 243 391 1500 1633 2691
Size: 243 148 1109 133 1058
Fragments arranged by size:
1109 1058 243 148 133
PasI CC'CwG_GG
Cuts at: 0 12 407 496 588 2386 2691
Size: 12 395 89 92 1798 305
Fragments arranged by size:
1798 395 305 92 89 12
PflMI CCAn_nnn'nTGG
Cuts at: 0 683 741 1188 1223 1648 1935 2691
Size: 683 58 447 35 425 287 756
Fragments arranged by size:
756 683 447 425 287 58 35
PfoI T'CCnGG_A
Cuts at: 0 206 2691
Size: 206 2485
PmlI CAC'GTG
Cuts at: 0 1145 2691
Size: 1145 1546
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1555 1587 2691
Size: 1555 32 1104
Fragments arranged by size:
1555 1104 32
PpuMI rG'GwC_Cy
Cuts at: 0 201 411 1244 1620 1871 2109 2527 2691
Size: 201 210 833 376 251 238 418 164
Fragments arranged by size:
833 418 376 251 238 210 201 164
PspOMI G'GGCC_C
Cuts at: 0 1771 2485 2691
Size: 1771 714 206
Fragments arranged by size:
1771 714 206
PstI C_TGCA'G
Cuts at: 0 92 136 419 1989 2145 2450 2691
Size: 92 44 283 1570 156 305 241
Fragments arranged by size:
1570 305 283 241 156 92 44
PvuII CAG'CTG
Cuts at: 0 776 1669 2691
Size: 776 893 1022
Fragments arranged by size:
1022 893 776
SacI G_AGCT'C
Cuts at: 0 1003 2095 2306 2691
Size: 1003 1092 211 385
Fragments arranged by size:
1092 1003 385 211
SanDI GG'GwC_CC
Cuts at: 0 1871 2527 2691
Size: 1871 656 164
Fragments arranged by size:
1871 656 164
SapI GCTCTTCn'nnn_
Cuts at: 0 2623 2691
Size: 2623 68
SbfI CC_TGCA'GG
Cuts at: 0 136 2691
Size: 136 2555
SexAI A'CCwGG_T
Cuts at: 0 1216 2147 2691
Size: 1216 931 544
Fragments arranged by size:
1216 931 544
SfcI C'TryA_G
Cuts at: 0 88 132 415 1059 1577 1985 2141 2446
Size: 88 44 283 644 518 408 156 305
Cuts at: 2446 2691
Size: 245
Fragments arranged by size:
644 518 408 305 283 245 156 88
44
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 37 2691
Size: 37 2654
SmaI CCC'GGG
Cuts at: 0 2525 2691
Size: 2525 166
StuI AGG'CCT
Cuts at: 0 351 2691
Size: 351 2340
StyI C'CwwG_G
Cuts at: 0 26 465 973 1043 2041 2338 2659 2691
Size: 26 439 508 70 998 297 321 32
Fragments arranged by size:
998 508 439 321 297 70 32 26
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 163 801 1177 1317 2691
Size: 163 638 376 140 1374
Fragments arranged by size:
1374 638 376 163 140
TatI w'GTAC_w
Cuts at: 0 1124 2691
Size: 1124 1567
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 475 507 847 879 1260 1292 1783 1815
Size: 475 32 340 32 381 32 491 32
Cuts at: 1815 1870 1902 1937 1969 2691
Size: 55 32 35 32 722
Fragments arranged by size:
722 491 475 381 340 55 35 32
32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 2531 2691
Size: 2531 160
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 33 698 2691
Size: 33 665 1993
Fragments arranged by size:
1993 665 33
XmaI C'CCGG_G
Cuts at: 0 2523 2691
Size: 2523 168
ZraI GAC'GTC
Cuts at: 0 673 2691
Size: 673 2018
Enzymes that do cut:
AarI AatII AccI AcuI AhdI AleI AlfI AloI
AlwNI ApaI AvaI BanI BanII BbvCI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BsaI BsaAI
BsaHI BsaWI BsaXI BseRI BseYI BsiHKAI BsmBI Bsp1286I
BspEI BspMI BsrBI BsrFI BssHII BstAPI BstEII BstYI
Bsu36I BtgI BtgZI BtsI EaeI EarI EcoICRI Eco57MI
EcoNI EcoO109I FspI Hin4I HincII MfeI MmeI MscI
MslI MspA1I NcoI NspI PasI PflMI PfoI PmlI
PpiI PpuMI PspOMI PstI PvuII SacI SanDI SapI
SbfI SexAI SfcI SfiI SmaI StuI StyI TaqII
TatI TstI Tth111I XcmI XmaI ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AflII AflIII AgeI ApaLI ApoI
AscI AseI AsiSI AvrII BaeI BamHI BbeI BbsI
BcgI BciVI BclI BfrBI BmgBI BmtI BpuEI BsaBI
BsgI BsiEI BsiWI BsmI BspHI BsrDI BsrGI BssSI
BstBI BstXI BstZ17I ClaI CspCI DraI DraIII DrdI
EagI EciI EcoRI EcoRV FalI FseI FspAI HaeII
HindIII HpaI KasI KpnI MluI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI PacI PciI PmeI
PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI
ScaI SfoI SgfI SgrAI SmlI SnaBI SpeI SphI
SrfI SspI SwaI XbaI XhoI XmnI