Restriction map for
pF1KB3347
(Linear) (Six-base) MAPSORT of: KIBB3347.seq Check: 3915 from: 1 to: 2211
KIBB3347.orf
With 182 enzymes: SgfI *
July 18, 2007 13:36 ..
AatII G_ACGT'C
Cuts at: 0 1244 2211
Size: 1244 967
AclI AA'CG_TT
Cuts at: 0 118 2211
Size: 118 2093
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 241 845 1828 1970 2211
Size: 241 604 983 142 241
Fragments arranged by size:
983 604 241 241 142
AflII C'TTAA_G
Cuts at: 0 27 2211
Size: 27 2184
AlwNI CAG_nnn'CTG
Cuts at: 0 91 669 726 1065 1224 1389 1973 2211
Size: 91 578 57 339 159 165 584 238
Fragments arranged by size:
584 578 339 238 165 159 91 57
ApaI G_GGCC'C
Cuts at: 0 864 2211
Size: 864 1347
AvaI C'yCGr_G
Cuts at: 0 500 599 932 2211
Size: 500 99 333 1279
Fragments arranged by size:
1279 500 333 99
BanII G_rGCy'C
Cuts at: 0 326 864 933 939 1486 1649 1992 2211
Size: 326 538 69 6 547 163 343 219
Fragments arranged by size:
547 538 343 326 219 163 69 6
BbsI GAAGACnn'nnnn_
Cuts at: 0 833 1137 1816 2211
Size: 833 304 679 395
Fragments arranged by size:
833 679 395 304
BciVI GTATCCnnnnn_n'
Cuts at: 0 1551 2211
Size: 1551 660
BclI T'GATC_A
Cuts at: 0 941 2211
Size: 941 1270
BfrBI ATG'CAT
Cuts at: 0 1844 2211
Size: 1844 367
Bme1580I G_kGCm'C
Cuts at: 0 864 2211
Size: 864 1347
BmgBI CAC'GTC
Cuts at: 0 1430 1918 2211
Size: 1430 488 293
Fragments arranged by size:
1430 488 293
BmrI ACTGGGnnnn_n'
Cuts at: 0 320 533 2211
Size: 320 213 1678
Fragments arranged by size:
1678 320 213
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 748 1141 2211
Size: 748 393 1070
Fragments arranged by size:
1070 748 393
Bpu10I CC'TnA_GC
Cuts at: 0 104 1597 2211
Size: 104 1493 614
Fragments arranged by size:
1493 614 104
BsaI GGTCTCn'nnnn_
Cuts at: 0 349 995 1042 2211
Size: 349 646 47 1169
Fragments arranged by size:
1169 646 349 47
BsaBI GATnn'nnATC
Cuts at: 0 461 2105 2211
Size: 461 1644 106
Fragments arranged by size:
1644 461 106
BsaHI Gr'CG_yC
Cuts at: 0 1241 2211
Size: 1241 970
BsaWI w'CCGG_w
Cuts at: 0 1026 2211
Size: 1026 1185
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 60 715 1329 2211
Size: 60 655 614 882
Fragments arranged by size:
882 655 614 60
BseYI C'CCAG_C
Cuts at: 0 281 641 1630 1792 2211
Size: 281 360 989 162 419
Fragments arranged by size:
989 419 360 281 162
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 196 1300 1375 2211
Size: 196 1104 75 836
Fragments arranged by size:
1104 836 196 75
BsiHKAI G_wGCw'C
Cuts at: 0 482 933 1497 2211
Size: 482 451 564 714
Fragments arranged by size:
714 564 482 451
Bsp1286I G_dGCh'C
Cuts at: 0 326 482 864 933 939 1486 1497 1649
Size: 326 156 382 69 6 547 11 152
Cuts at: 1649 1992 2211
Size: 343 219
Fragments arranged by size:
547 382 343 326 219 156 152 69
11 6
BspHI T'CATG_A
Cuts at: 0 2205 2211
Size: 2205 6
BsrDI GCAATG_nn'
Cuts at: 0 564 1281 1730 2211
Size: 564 717 449 481
Fragments arranged by size:
717 564 481 449
BsrFI r'CCGG_y
Cuts at: 0 32 2211
Size: 32 2179
BsrGI T'GTAC_A
Cuts at: 0 1982 2211
Size: 1982 229
BssHII G'CGCG_C
Cuts at: 0 495 2211
Size: 495 1716
BssSI C'ACGA_G
Cuts at: 0 1563 2211
Size: 1563 648
BstAPI GCAn_nnn'nTGC
Cuts at: 0 100 2211
Size: 100 2111
BstEII G'GTnAC_C
Cuts at: 0 814 1938 2211
Size: 814 1124 273
Fragments arranged by size:
1124 814 273
BstYI r'GATC_y
Cuts at: 0 845 1160 2211
Size: 845 315 1051
Fragments arranged by size:
1051 845 315
Bsu36I CC'TnA_GG
Cuts at: 0 1139 2211
Size: 1139 1072
BtgI C'CryG_G
Cuts at: 0 1005 1415 2211
Size: 1005 410 796
Fragments arranged by size:
1005 796 410
BtsI GCAGTG_nn'
Cuts at: 0 1070 1234 1312 2211
Size: 1070 164 78 899
Fragments arranged by size:
1070 899 164 78
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 294 329 1591 1626 2211
Size: 294 35 1262 35 585
Fragments arranged by size:
1262 585 294 35 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 1099 1581 2211
Size: 1099 482 630
Fragments arranged by size:
1099 630 482
EaeI y'GGCC_r
Cuts at: 0 1115 1286 1374 2211
Size: 1115 171 88 837
Fragments arranged by size:
1115 837 171 88
EarI CTCTTCn'nnn_
Cuts at: 0 45 217 399 1946 2211
Size: 45 172 182 1547 265
Fragments arranged by size:
1547 265 182 172 45
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1429 2211
Size: 1429 782
EcoICRI GAG'CTC
Cuts at: 0 931 2211
Size: 931 1280
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 241 748 845 1141 1828 1970 2211
Size: 241 507 97 296 687 142 241
Fragments arranged by size:
687 507 296 241 241 142 97
EcoNI CCTnn'n_nnAGG
Cuts at: 0 260 2211
Size: 260 1951
EcoO109I rG'GnC_Cy
Cuts at: 0 860 2211
Size: 860 1351
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1833 2211
Size: 1833 378
HaeII r_GCGC'y
Cuts at: 0 708 2211
Size: 708 1503
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 926 958 1166 1198 1455 1487 1513 1545
Size: 926 32 208 32 257 32 26 32
Cuts at: 1545 1572 1604 2211
Size: 27 32 607
Fragments arranged by size:
926 607 257 208 32 32 32 32
32 27 26
HincII GTy'rAC
Cuts at: 0 57 2211
Size: 57 2154
HindIII A'AGCT_T
Cuts at: 0 634 2211
Size: 634 1577
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 855 1847 2211
Size: 855 992 364
Fragments arranged by size:
992 855 364
MscI TGG'CCA
Cuts at: 0 1288 2211
Size: 1288 923
MslI CAynn'nnrTG
Cuts at: 0 64 185 1075 1900 2211
Size: 64 121 890 825 311
Fragments arranged by size:
890 825 311 121 64
MspA1I CmG'CkG
Cuts at: 0 285 341 669 714 726 747 756 1369
Size: 285 56 328 45 12 21 9 613
Cuts at: 1369 2211
Size: 842
Fragments arranged by size:
842 613 328 285 56 45 21 12
9
NaeI GCC'GGC
Cuts at: 0 34 2211
Size: 34 2177
NcoI C'CATG_G
Cuts at: 0 1005 2211
Size: 1005 1206
NgoMIV G'CCGG_C
Cuts at: 0 32 2211
Size: 32 2179
NsiI A_TGCA'T
Cuts at: 0 1846 2211
Size: 1846 365
NspI r_CATG'y
Cuts at: 0 184 738 837 2001 2211
Size: 184 554 99 1164 210
Fragments arranged by size:
1164 554 210 184 99
PflMI CCAn_nnn'nTGG
Cuts at: 0 669 2211
Size: 669 1542
PfoI T'CCnGG_A
Cuts at: 0 261 848 2042 2113 2211
Size: 261 587 1194 71 98
Fragments arranged by size:
1194 587 261 98 71
PspOMI G'GGCC_C
Cuts at: 0 860 2211
Size: 860 1351
PspXI vC'TCGA_Gb
Cuts at: 0 932 2211
Size: 932 1279
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1987 2019 2211
Size: 1987 32 192
Fragments arranged by size:
1987 192 32
PstI C_TGCA'G
Cuts at: 0 761 1229 2211
Size: 761 468 982
Fragments arranged by size:
982 761 468
PvuII CAG'CTG
Cuts at: 0 285 669 714 726 747 756 2211
Size: 285 384 45 12 21 9 1455
Fragments arranged by size:
1455 384 285 45 21 12 9
SacI G_AGCT'C
Cuts at: 0 933 2211
Size: 933 1278
SapI GCTCTTCn'nnn_
Cuts at: 0 1946 2211
Size: 1946 265
ScaI AGT'ACT
Cuts at: 0 1947 2211
Size: 1947 264
SfcI C'TryA_G
Cuts at: 0 757 1225 2211
Size: 757 468 986
Fragments arranged by size:
986 757 468
SmaI CCC'GGG
Cuts at: 0 502 2211
Size: 502 1709
SmlI C'TyrA_G
Cuts at: 0 27 932 2211
Size: 27 905 1279
Fragments arranged by size:
1279 905 27
SphI G_CATG'C
Cuts at: 0 184 837 2211
Size: 184 653 1374
Fragments arranged by size:
1374 653 184
StuI AGG'CCT
Cuts at: 0 438 2211
Size: 438 1773
StyI C'CwwG_G
Cuts at: 0 141 1005 2211
Size: 141 864 1206
Fragments arranged by size:
1206 864 141
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 941 1758 2211
Size: 941 817 453
Fragments arranged by size:
941 817 453
TatI w'GTAC_w
Cuts at: 0 204 352 366 1945 1982 2211
Size: 204 148 14 1579 37 229
Fragments arranged by size:
1579 229 204 148 37 14
Tth111I GACn'n_nGTC
Cuts at: 0 871 2211
Size: 871 1340
XhoI C'TCGA_G
Cuts at: 0 932 2211
Size: 932 1279
XmaI C'CCGG_G
Cuts at: 0 500 2211
Size: 500 1711
ZraI GAC'GTC
Cuts at: 0 1242 2211
Size: 1242 969
Enzymes that do cut:
AatII AclI AcuI AflII AlwNI ApaI AvaI BanII
BbsI BciVI BclI BfrBI Bme1580I BmgBI BmrI BpmI
Bpu10I BsaI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiHKAI Bsp1286I BspHI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstEII BstYI Bsu36I BtgI BtsI CspCI DrdI
EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI
HaeII Hin4I HincII HindIII MmeI MscI MslI MspA1I
NaeI NcoI NgoMIV NsiI NspI PflMI PfoI PspOMI
PspXI PsrI PstI PvuII SacI SapI ScaI SfcI
SmaI SmlI SphI StuI StyI TaqII TatI Tth111I
XhoI XmaI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AfeI AflIII AgeI AhdI AleI
AlfI AloI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BanI BbeI BbvCI BcgI BglI BglII
BlpI BmtI BplI BpuEI BsaAI BsaXI BsiEI BsiWI
BsmI BsmBI BspEI BspMI BsrBI BstBI BstXI BstZ17I
BtgZI ClaI DraI DraIII EagI EcoRI EcoRV FseI
FspI FspAI HpaI KasI KpnI MfeI MluI NarI
NdeI NheI NotI NruI PacI PasI PciI PmeI
PmlI PpiI PpuMI PshAI PsiI PvuI RsrII SacII
SalI SanDI SbfI SexAI SfiI SfoI SgfI SgrAI
SnaBI SpeI SrfI SspI SwaI TstI XbaI XcmI
XmnI