Restriction map for
pF1KB3349
(Linear) (Six-base) MAPSORT of: KIBB3349.seq Check: 9598 from: 1 to: 4581
KIBB3349.orf
With 182 enzymes: SgfI *
February 28, 2007 11:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1527 2787 3693 4217 4581
Size: 1527 1260 906 524 364
Fragments arranged by size:
1527 1260 906 524 364
AccI GT'mk_AC
Cuts at: 0 605 3009 4581
Size: 605 2404 1572
Fragments arranged by size:
2404 1572 605
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 552 800 919 1522 1552 2098 3427 4581
Size: 552 248 119 603 30 546 1329 1154
Fragments arranged by size:
1329 1154 603 552 546 248 119 30
AfeI AGC'GCT
Cuts at: 0 633 1255 2155 3379 4581
Size: 633 622 900 1224 1202
Fragments arranged by size:
1224 1202 900 633 622
AflII C'TTAA_G
Cuts at: 0 1731 4581
Size: 1731 2850
AflIII A'CryG_T
Cuts at: 0 1661 4214 4581
Size: 1661 2553 367
Fragments arranged by size:
2553 1661 367
AgeI A'CCGG_T
Cuts at: 0 3575 4581
Size: 3575 1006
AhdI GACnn_n'nnGTC
Cuts at: 0 720 2331 4581
Size: 720 1611 2250
Fragments arranged by size:
2250 1611 720
AleI CACnn'nnGTG
Cuts at: 0 2689 3301 3370 4219 4581
Size: 2689 612 69 849 362
Fragments arranged by size:
2689 849 612 362 69
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1513 4581
Size: 1513 3068
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3007 3039 3199 3231 3853 3885 4581
Size: 3007 32 160 32 622 32 696
Fragments arranged by size:
3007 696 622 160 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 765 768 826 944 966 1092 1572 1917
Size: 765 3 58 118 22 126 480 345
Cuts at: 1917 2169 2981 3141 3466 4581
Size: 252 812 160 325 1115
Fragments arranged by size:
1115 812 765 480 345 325 252 160
126 118 58 22 3
ApaI G_GGCC'C
Cuts at: 0 300 1911 2500 4581
Size: 300 1611 589 2081
Fragments arranged by size:
2081 1611 589 300
ApaLI G'TGCA_C
Cuts at: 0 61 2053 3937 4581
Size: 61 1992 1884 644
Fragments arranged by size:
1992 1884 644 61
ApoI r'AATT_y
Cuts at: 0 3056 4507 4581
Size: 3056 1451 74
Fragments arranged by size:
3056 1451 74
AvaI C'yCGr_G
Cuts at: 0 2285 3666 4128 4310 4581
Size: 2285 1381 462 182 271
Fragments arranged by size:
2285 1381 462 271 182
AvrII C'CTAG_G
Cuts at: 0 1341 4581
Size: 1341 3240
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 3515 3548 4581
Size: 3515 33 1033
Fragments arranged by size:
3515 1033 33
BamHI G'GATC_C
Cuts at: 0 1802 2096 4581
Size: 1802 294 2485
Fragments arranged by size:
2485 1802 294
BanI G'GyrC_C
Cuts at: 0 117 170 865 1894 2576 2771 3469 3807
Size: 117 53 695 1029 682 195 698 338
Cuts at: 3807 4581
Size: 774
Fragments arranged by size:
1029 774 698 695 682 338 195 117
53
BanII G_rGCy'C
Cuts at: 0 29 300 1523 1565 1911 2068 2500 2867
Size: 29 271 1223 42 346 157 432 367
Cuts at: 2867 2903 4228 4317 4581
Size: 36 1325 89 264
Fragments arranged by size:
1325 1223 432 367 346 271 264 157
89 42 36 29
BbeI G_GCGC'C
Cuts at: 0 3811 4581
Size: 3811 770
BbsI GAAGACnn'nnnn_
Cuts at: 0 389 2645 3980 4581
Size: 389 2256 1335 601
Fragments arranged by size:
2256 1335 601 389
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 160 194 4581
Size: 160 34 4387
Fragments arranged by size:
4387 160 34
BclI T'GATC_A
Cuts at: 0 1004 1643 4581
Size: 1004 639 2938
Fragments arranged by size:
2938 1004 639
BglI GCCn_nnn'nGGC
Cuts at: 0 910 1699 4581
Size: 910 789 2882
Fragments arranged by size:
2882 910 789
BglII A'GATC_T
Cuts at: 0 491 4581
Size: 491 4090
BlpI GC'TnA_GC
Cuts at: 0 994 3583 4581
Size: 994 2589 998
Fragments arranged by size:
2589 998 994
Bme1580I G_kGCm'C
Cuts at: 0 65 122 300 870 1911 1965 2057 2084
Size: 65 57 178 570 1041 54 92 27
Cuts at: 2084 2500 2579 2727 2826 3941 4581
Size: 416 79 148 99 1115 640
Fragments arranged by size:
1115 1041 640 570 416 178 148 99
92 79 65 57 54 27
BmgBI CAC'GTC
Cuts at: 0 2376 4581
Size: 2376 2205
BmrI ACTGGGnnnn_n'
Cuts at: 0 1139 1157 1752 3983 4404 4581
Size: 1139 18 595 2231 421 177
Fragments arranged by size:
2231 1139 595 421 177 18
BmtI G_CTAG'C
Cuts at: 0 2191 2473 4581
Size: 2191 282 2108
Fragments arranged by size:
2191 2108 282
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 48 4336 4581
Size: 48 4288 245
Fragments arranged by size:
4288 245 48
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 724 790 909 1382 1549 2191 2218 2602
Size: 724 66 119 473 167 642 27 384
Cuts at: 2602 4039 4393 4502 4581
Size: 1437 354 109 79
Fragments arranged by size:
1437 724 642 473 384 354 167 119
109 79 66 27
Bpu10I CC'TnA_GC
Cuts at: 0 1816 4581
Size: 1816 2765
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 199 4581
Size: 199 4382
BsaI GGTCTCn'nnnn_
Cuts at: 0 619 3914 4581
Size: 619 3295 667
Fragments arranged by size:
3295 667 619
BsaAI yAC'GTr
Cuts at: 0 1662 1668 3856 4581
Size: 1662 6 2188 725
Fragments arranged by size:
2188 1662 725 6
BsaBI GATnn'nnATC
Cuts at: 0 679 4581
Size: 679 3902
BsaHI Gr'CG_yC
Cuts at: 0 5 1691 3038 3808 4581
Size: 5 1686 1347 770 773
Fragments arranged by size:
1686 1347 773 770 5
BsaWI w'CCGG_w
Cuts at: 0 3575 3872 4581
Size: 3575 297 709
Fragments arranged by size:
3575 709 297
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 955 985 2951 2981 3199 3229 3277 3307
Size: 955 30 1966 30 218 30 48 30
Cuts at: 3307 3553 3583 3853 3883 4581
Size: 246 30 270 30 698
Fragments arranged by size:
1966 955 698 270 246 218 48 30
30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 721 2851 3666 4189 4581
Size: 721 2130 815 523 392
Fragments arranged by size:
2130 815 721 523 392
BseYI C'CCAG_C
Cuts at: 0 861 1057 1250 1522 2022 2084 2505 3033
Size: 861 196 193 272 500 62 421 528
Cuts at: 3033 4229 4581
Size: 1196 352
Fragments arranged by size:
1196 861 528 500 421 352 272 196
193 62
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 778 1641 1915 2091 2630 2815 3394 3614
Size: 778 863 274 176 539 185 579 220
Cuts at: 3614 4195 4407 4581
Size: 581 212 174
Fragments arranged by size:
863 778 581 579 539 274 220 212
185 176 174
BsiEI CG_ry'CG
Cuts at: 0 3898 4581
Size: 3898 683
BsiHKAI G_wGCw'C
Cuts at: 0 29 65 1078 1565 2057 2867 3362 3623
Size: 29 36 1013 487 492 810 495 261
Cuts at: 3623 3941 4228 4258 4581
Size: 318 287 30 323
Fragments arranged by size:
1013 810 495 492 487 323 318 287
261 36 30 29
BsmBI CGTCTCn'nnnn_
Cuts at: 0 252 4581
Size: 252 4329
Bsp1286I G_dGCh'C
Cuts at: 0 29 65 122 300 870 1078 1523 1565
Size: 29 36 57 178 570 208 445 42
Cuts at: 1565 1911 1965 2057 2068 2084 2500 2579 2727
Size: 346 54 92 11 16 416 79 148
Cuts at: 2727 2826 2867 2903 3362 3623 3941 4228 4258
Size: 99 41 36 459 261 318 287 30
Cuts at: 4258 4317 4581
Size: 59 264
Fragments arranged by size:
570 459 445 416 346 318 287 264
261 208 178 148 99 92 79 59
57 54 42 41 36 36 30 29
16 11
BspEI T'CCGG_A
Cuts at: 0 3872 4581
Size: 3872 709
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 173 974 1527 1925 2787 3693 4091 4217
Size: 173 801 553 398 862 906 398 126
Cuts at: 4217 4429 4581
Size: 212 152
Fragments arranged by size:
906 862 801 553 398 398 212 173
152 126
BsrBI CCG'CTC
Cuts at: 0 378 458 3327 3431 4581
Size: 378 80 2869 104 1150
Fragments arranged by size:
2869 1150 378 104 80
BsrDI GCAATG_nn'
Cuts at: 0 4529 4581
Size: 4529 52
BsrFI r'CCGG_y
Cuts at: 0 692 3575 3893 4233 4445 4581
Size: 692 2883 318 340 212 136
Fragments arranged by size:
2883 692 340 318 212 136
BssHII G'CGCG_C
Cuts at: 0 1419 4581
Size: 1419 3162
BssSI C'ACGA_G
Cuts at: 0 4297 4581
Size: 4297 284
BstAPI GCAn_nnn'nTGC
Cuts at: 0 765 3637 4581
Size: 765 2872 944
Fragments arranged by size:
2872 944 765
BstEII G'GTnAC_C
Cuts at: 0 339 1635 4581
Size: 339 1296 2946
Fragments arranged by size:
2946 1296 339
BstXI CCAn_nnnn'nTGG
Cuts at: 0 181 351 1026 2895 3096 3303 4371 4479
Size: 181 170 675 1869 201 207 1068 108
Cuts at: 4479 4581
Size: 102
Fragments arranged by size:
1869 1068 675 207 201 181 170 108
102
BstYI r'GATC_y
Cuts at: 0 464 491 742 1478 1802 2096 2661 4581
Size: 464 27 251 736 324 294 565 1920
Fragments arranged by size:
1920 736 565 464 324 294 251 27
Bsu36I CC'TnA_GG
Cuts at: 0 3028 4581
Size: 3028 1553
BtgI C'CryG_G
Cuts at: 0 276 288 1025 1973 2069 2336 2894 2996
Size: 276 12 737 948 96 267 558 102
Cuts at: 2996 3089 3095 4581
Size: 93 6 1486
Fragments arranged by size:
1486 948 737 558 276 267 102 96
93 12 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 4422 4581
Size: 4422 159
BtsI GCAGTG_nn'
Cuts at: 0 2626 3139 4581
Size: 2626 513 1442
Fragments arranged by size:
2626 1442 513
DrdI GACnn_nn'nnGTC
Cuts at: 0 1948 3229 4581
Size: 1948 1281 1352
Fragments arranged by size:
1948 1352 1281
EaeI y'GGCC_r
Cuts at: 0 291 353 677 911 1406 1970 2354 2915
Size: 291 62 324 234 495 564 384 561
Cuts at: 2915 3092 3314 3895 4559 4581
Size: 177 222 581 664 22
Fragments arranged by size:
664 581 564 561 495 384 324 291
234 222 177 62 22
EagI C'GGCC_G
Cuts at: 0 3895 4581
Size: 3895 686
EarI CTCTTCn'nnn_
Cuts at: 0 728 2117 3334 4581
Size: 728 1389 1217 1247
Fragments arranged by size:
1389 1247 1217 728
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 271 4581
Size: 271 4310
EcoICRI GAG'CTC
Cuts at: 0 27 1563 2865 4226 4581
Size: 27 1536 1302 1361 355
Fragments arranged by size:
1536 1361 1302 355 27
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 552 724 790 800 909 919 1382 1522
Size: 552 172 66 10 109 10 463 140
Cuts at: 1522 1549 1552 2098 2191 2218 2602 3427 4039
Size: 27 3 546 93 27 384 825 612
Cuts at: 4039 4393 4502 4581
Size: 354 109 79
Fragments arranged by size:
825 612 552 546 463 384 354 172
140 109 109 93 79 66 27 27
10 10 3
EcoNI CCTnn'n_nnAGG
Cuts at: 0 567 1534 1777 3839 4390 4581
Size: 567 967 243 2062 551 191
Fragments arranged by size:
2062 967 567 551 243 191
EcoO109I rG'GnC_Cy
Cuts at: 0 297 405 714 878 903 963 1345 1914
Size: 297 108 309 164 25 60 382 569
Cuts at: 1914 1950 1981 4113 4133 4476 4581
Size: 36 31 2132 20 343 105
Fragments arranged by size:
2132 569 382 343 309 297 164 108
105 60 36 31 25 20
EcoRI G'AATT_C
Cuts at: 0 3056 4581
Size: 3056 1525
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 909 1846 2382 4581
Size: 909 937 536 2199
Fragments arranged by size:
2199 937 909 536
FspI TGC'GCA
Cuts at: 0 1576 4141 4581
Size: 1576 2565 440
Fragments arranged by size:
2565 1576 440
HaeII r_GCGC'y
Cuts at: 0 635 1257 2157 3381 3811 4581
Size: 635 622 900 1224 430 770
Fragments arranged by size:
1224 900 770 635 622 430
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 706 727 738 759 955 987 1727 1759
Size: 706 21 11 21 196 32 740 32
Cuts at: 1759 2317 2349 3699 3731 3736 3768 4072 4104
Size: 558 32 1350 32 5 32 304 32
Cuts at: 4104 4581
Size: 477
Fragments arranged by size:
1350 740 706 558 477 304 196 32
32 32 32 32 32 21 21 11
5
HincII GTy'rAC
Cuts at: 0 606 1230 4581
Size: 606 624 3351
Fragments arranged by size:
3351 624 606
HindIII A'AGCT_T
Cuts at: 0 952 4581
Size: 952 3629
KasI G'GCGC_C
Cuts at: 0 3807 4581
Size: 3807 774
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 74 1043 2582 3596 4581
Size: 74 969 1539 1014 985
Fragments arranged by size:
1539 1014 985 969 74
MscI TGG'CCA
Cuts at: 0 355 679 913 2356 2917 3094 3316 4561
Size: 355 324 234 1443 561 177 222 1245
Cuts at: 4561 4581
Size: 20
Fragments arranged by size:
1443 1245 561 355 324 234 222 177
20
MslI CAynn'nnrTG
Cuts at: 0 179 673 1123 1240 1495 1615 2464 2689
Size: 179 494 450 117 255 120 849 225
Cuts at: 2689 3094 3262 3301 3370 3592 4219 4459 4581
Size: 405 168 39 69 222 627 240 122
Fragments arranged by size:
849 627 494 450 405 255 240 225
222 179 168 122 120 117 69 39
MspA1I CmG'CkG
Cuts at: 0 765 990 1569 2265 2271 2338 2591 2718
Size: 765 225 579 696 6 67 253 127
Cuts at: 2718 3103 4095 4360 4581
Size: 385 992 265 221
Fragments arranged by size:
992 765 696 579 385 265 253 225
221 127 67 6
NaeI GCC'GGC
Cuts at: 0 4235 4581
Size: 4235 346
NarI GG'CG_CC
Cuts at: 0 3808 4581
Size: 3808 773
NcoI C'CATG_G
Cuts at: 0 276 288 1025 2996 3089 3095 4581
Size: 276 12 737 1971 93 6 1486
Fragments arranged by size:
1971 1486 737 276 93 12 6
NgoMIV G'CCGG_C
Cuts at: 0 4233 4581
Size: 4233 348
NheI G'CTAG_C
Cuts at: 0 2187 2469 4581
Size: 2187 282 2112
Fragments arranged by size:
2187 2112 282
NspI r_CATG'y
Cuts at: 0 1752 3937 4581
Size: 1752 2185 644
Fragments arranged by size:
2185 1752 644
PasI CC'CwG_GG
Cuts at: 0 3812 4581
Size: 3812 769
PflMI CCAn_nnn'nTGG
Cuts at: 0 41 282 753 1845 2969 3578 4581
Size: 41 241 471 1092 1124 609 1003
Fragments arranged by size:
1124 1092 1003 609 471 241 41
PfoI T'CCnGG_A
Cuts at: 0 959 4016 4484 4581
Size: 959 3057 468 97
Fragments arranged by size:
3057 959 468 97
PmlI CAC'GTG
Cuts at: 0 3856 4581
Size: 3856 725
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3199 3231 3853 3885 4581
Size: 3199 32 622 32 696
Fragments arranged by size:
3199 696 622 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 405 714 963 1345 1914 1950 4113 4133
Size: 405 309 249 382 569 36 2163 20
Cuts at: 4133 4476 4581
Size: 343 105
Fragments arranged by size:
2163 569 405 382 343 309 249 105
36 20
PshAI GACnn'nnGTC
Cuts at: 0 2484 3926 4581
Size: 2484 1442 655
Fragments arranged by size:
2484 1442 655
PspOMI G'GGCC_C
Cuts at: 0 296 1907 2496 4581
Size: 296 1611 589 2085
Fragments arranged by size:
2085 1611 589 296
PspXI vC'TCGA_Gb
Cuts at: 0 2285 4581
Size: 2285 2296
PstI C_TGCA'G
Cuts at: 0 386 1307 2519 4581
Size: 386 921 1212 2062
Fragments arranged by size:
2062 1212 921 386
PvuII CAG'CTG
Cuts at: 0 765 990 1569 2591 2718 4095 4360 4581
Size: 765 225 579 1022 127 1377 265 221
Fragments arranged by size:
1377 1022 765 579 265 225 221 127
SacI G_AGCT'C
Cuts at: 0 29 1565 2867 4228 4581
Size: 29 1536 1302 1361 353
Fragments arranged by size:
1536 1361 1302 353 29
SacII CC_GC'GG
Cuts at: 0 2339 4581
Size: 2339 2242
SalI G'TCGA_C
Cuts at: 0 604 4581
Size: 604 3977
SanDI GG'GwC_CC
Cuts at: 0 4133 4581
Size: 4133 448
SapI GCTCTTCn'nnn_
Cuts at: 0 3334 4581
Size: 3334 1247
ScaI AGT'ACT
Cuts at: 0 3787 4581
Size: 3787 794
SexAI A'CCwGG_T
Cuts at: 0 3908 4581
Size: 3908 673
SfcI C'TryA_G
Cuts at: 0 382 1167 1303 2515 4581
Size: 382 785 136 1212 2066
Fragments arranged by size:
2066 1212 785 382 136
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 910 4581
Size: 910 3671
SfoI GGC'GCC
Cuts at: 0 3809 4581
Size: 3809 772
SmaI CCC'GGG
Cuts at: 0 3668 4581
Size: 3668 913
SmlI C'TyrA_G
Cuts at: 0 214 1731 2285 4581
Size: 214 1517 554 2296
Fragments arranged by size:
2296 1517 554 214
StuI AGG'CCT
Cuts at: 0 1702 2176 4241 4581
Size: 1702 474 2065 340
Fragments arranged by size:
2065 1702 474 340
StyI C'CwwG_G
Cuts at: 0 276 288 326 476 1025 1341 2571 2888
Size: 276 12 38 150 549 316 1230 317
Cuts at: 2888 2996 3089 3095 3218 4581
Size: 108 93 6 123 1363
Fragments arranged by size:
1363 1230 549 317 316 276 150 123
108 93 38 12 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 105 217 853 1147 1644 3804 4423 4581
Size: 105 112 636 294 497 2160 619 158
Fragments arranged by size:
2160 636 619 497 294 158 112 105
TatI w'GTAC_w
Cuts at: 0 3785 4581
Size: 3785 796
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 27 1143 1175 2951 2983 4171 4203 4237
Size: 27 1116 32 1776 32 1188 32 34
Cuts at: 4237 4269 4581
Size: 32 312
Fragments arranged by size:
1776 1188 1116 312 34 32 32 32
32 27
Tth111I GACn'n_nGTC
Cuts at: 0 2374 2750 4581
Size: 2374 376 1831
Fragments arranged by size:
2374 1831 376
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 216 2856 4581
Size: 216 2640 1725
Fragments arranged by size:
2640 1725 216
XhoI C'TCGA_G
Cuts at: 0 2285 4581
Size: 2285 2296
XmaI C'CCGG_G
Cuts at: 0 3666 4581
Size: 3666 915
XmnI GAAnn'nnTTC
Cuts at: 0 3211 4581
Size: 3211 1370
Enzymes that do cut:
AarI AccI AcuI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AvaI
AvrII BaeI BamHI BanI BanII BbeI BbsI BcgI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BssHII BssSI
BstAPI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NheI NspI PasI PflMI PfoI PmlI PpiI
PpuMI PshAI PspOMI PspXI PstI PvuII SacI SacII
SalI SanDI SapI ScaI SexAI SfcI SfiI SfoI
SmaI SmlI StuI StyI TaqII TatI TstI Tth111I
XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AscI AseI AsiSI BbvCI BciVI
BfrBI BsiWI BsmI BspHI BsrGI BstBI BstZ17I ClaI
CspCI DraI DraIII EcoRV FseI FspAI HpaI KpnI
MfeI MluI NdeI NotI NruI NsiI PacI PciI
PmeI PsiI PsrI PvuI RsrII SbfI SgfI SgrAI
SnaBI SpeI SphI SrfI SspI SwaI XbaI ZraI