Restriction map for
pF1KB3372
(Linear) (Six-base) MAPSORT of: KIBB3372.seq Check: 1516 from: 1 to: 1335
KIBB3372.orf
With 182 enzymes: SgfI *
March 3, 2008 10:56 ..
AccI GT'mk_AC
Cuts at: 0 190 677 1335
Size: 190 487 658
Fragments arranged by size:
658 487 190
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 172 891 1335
Size: 172 719 444
Fragments arranged by size:
719 444 172
AflII C'TTAA_G
Cuts at: 0 1056 1335
Size: 1056 279
AflIII A'CryG_T
Cuts at: 0 446 1335
Size: 446 889
AhdI GACnn_n'nnGTC
Cuts at: 0 186 1335
Size: 186 1149
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 599 1335
Size: 599 736
AlwNI CAG_nnn'CTG
Cuts at: 0 465 1335
Size: 465 870
ApoI r'AATT_y
Cuts at: 0 47 1078 1335
Size: 47 1031 257
Fragments arranged by size:
1031 257 47
AvaI C'yCGr_G
Cuts at: 0 516 1335
Size: 516 819
BanI G'GyrC_C
Cuts at: 0 232 1144 1335
Size: 232 912 191
Fragments arranged by size:
912 232 191
BanII G_rGCy'C
Cuts at: 0 356 905 1139 1335
Size: 356 549 234 196
Fragments arranged by size:
549 356 234 196
BbeI G_GCGC'C
Cuts at: 0 236 1335
Size: 236 1099
BciVI GTATCCnnnnn_n'
Cuts at: 0 70 860 1335
Size: 70 790 475
Fragments arranged by size:
790 475 70
BglI GCCn_nnn'nGGC
Cuts at: 0 169 557 1335
Size: 169 388 778
Fragments arranged by size:
778 388 169
BmgBI CAC'GTC
Cuts at: 0 888 1335
Size: 888 447
BmrI ACTGGGnnnn_n'
Cuts at: 0 1228 1335
Size: 1228 107
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 531 1335
Size: 531 804
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 295 1012 1169 1244 1335
Size: 295 717 157 75 91
Fragments arranged by size:
717 295 157 91 75
Bpu10I CC'TnA_GC
Cuts at: 0 840 917 1133 1335
Size: 840 77 216 202
Fragments arranged by size:
840 216 202 77
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 838 1335
Size: 838 497
BsaAI yAC'GTr
Cuts at: 0 449 1335
Size: 449 886
BsaBI GATnn'nnATC
Cuts at: 0 145 1335
Size: 145 1190
BsaHI Gr'CG_yC
Cuts at: 0 233 985 1335
Size: 233 752 350
Fragments arranged by size:
752 350 233
BsaWI w'CCGG_w
Cuts at: 0 437 1335
Size: 437 898
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 700 730 1335
Size: 700 30 605
Fragments arranged by size:
700 605 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 947 1335
Size: 947 388
BseYI C'CCAG_C
Cuts at: 0 546 1335
Size: 546 789
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 73 114 688 936 1335
Size: 73 41 574 248 399
Fragments arranged by size:
574 399 248 73 41
BsiEI CG_ry'CG
Cuts at: 0 123 1335
Size: 123 1212
BsiHKAI G_wGCw'C
Cuts at: 0 356 1139 1322 1335
Size: 356 783 183 13
Fragments arranged by size:
783 356 183 13
BsmI GAATG_Cn'
Cuts at: 0 793 1335
Size: 793 542
BsmBI CGTCTCn'nnnn_
Cuts at: 0 535 720 1335
Size: 535 185 615
Fragments arranged by size:
615 535 185
Bsp1286I G_dGCh'C
Cuts at: 0 356 905 1139 1322 1335
Size: 356 549 234 183 13
Fragments arranged by size:
549 356 234 183 13
BspHI T'CATG_A
Cuts at: 0 74 1335
Size: 74 1261
BsrBI CCG'CTC
Cuts at: 0 523 654 1335
Size: 523 131 681
Fragments arranged by size:
681 523 131
BsrFI r'CCGG_y
Cuts at: 0 346 1335
Size: 346 989
BssSI C'ACGA_G
Cuts at: 0 205 1335
Size: 205 1130
BstAPI GCAn_nnn'nTGC
Cuts at: 0 693 1335
Size: 693 642
BstYI r'GATC_y
Cuts at: 0 500 1019 1335
Size: 500 519 316
Fragments arranged by size:
519 500 316
BtgI C'CryG_G
Cuts at: 0 227 389 534 813 1205 1335
Size: 227 162 145 279 392 130
Fragments arranged by size:
392 279 227 162 145 130
BtsI GCAGTG_nn'
Cuts at: 0 321 463 723 962 1335
Size: 321 142 260 239 373
Fragments arranged by size:
373 321 260 239 142
EaeI y'GGCC_r
Cuts at: 0 120 161 1335
Size: 120 41 1174
Fragments arranged by size:
1174 120 41
EagI C'GGCC_G
Cuts at: 0 120 1335
Size: 120 1215
EarI CTCTTCn'nnn_
Cuts at: 0 389 1335
Size: 389 946
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 52 1012 1151 1335
Size: 52 960 139 184
Fragments arranged by size:
960 184 139 52
EcoICRI GAG'CTC
Cuts at: 0 354 1137 1335
Size: 354 783 198
Fragments arranged by size:
783 354 198
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 172 295 891 1012 1169 1244 1335
Size: 172 123 596 121 157 75 91
Fragments arranged by size:
596 172 157 123 121 91 75
EcoNI CCTnn'n_nnAGG
Cuts at: 0 856 1335
Size: 856 479
EcoO109I rG'GnC_Cy
Cuts at: 0 156 270 288 1335
Size: 156 114 18 1047
Fragments arranged by size:
1047 156 114 18
EcoRI G'AATT_C
Cuts at: 0 1078 1335
Size: 1078 257
EcoRV GAT'ATC
Cuts at: 0 1159 1335
Size: 1159 176
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1098 1335
Size: 1098 237
HaeII r_GCGC'y
Cuts at: 0 112 236 1099 1279 1335
Size: 112 124 863 180 56
Fragments arranged by size:
863 180 124 112 56
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 172 204 599 631 700 732 970 1002
Size: 172 32 395 32 69 32 238 32
Cuts at: 1002 1335
Size: 333
Fragments arranged by size:
395 333 238 172 69 32 32 32
32
HincII GTy'rAC
Cuts at: 0 191 1335
Size: 191 1144
HindIII A'AGCT_T
Cuts at: 0 1053 1335
Size: 1053 282
KasI G'GCGC_C
Cuts at: 0 232 1335
Size: 232 1103
MscI TGG'CCA
Cuts at: 0 163 1335
Size: 163 1172
MslI CAynn'nnrTG
Cuts at: 0 1234 1335
Size: 1234 101
MspA1I CmG'CkG
Cuts at: 0 403 1009 1335
Size: 403 606 326
Fragments arranged by size:
606 403 326
NaeI GCC'GGC
Cuts at: 0 348 1335
Size: 348 987
NarI GG'CG_CC
Cuts at: 0 233 1335
Size: 233 1102
NcoI C'CATG_G
Cuts at: 0 1205 1335
Size: 1205 130
NgoMIV G'CCGG_C
Cuts at: 0 346 1335
Size: 346 989
PasI CC'CwG_GG
Cuts at: 0 660 1335
Size: 660 675
PfoI T'CCnGG_A
Cuts at: 0 1185 1335
Size: 1185 150
PmlI CAC'GTG
Cuts at: 0 449 1335
Size: 449 886
PpuMI rG'GwC_Cy
Cuts at: 0 156 270 1335
Size: 156 114 1065
Fragments arranged by size:
1065 156 114
PstI C_TGCA'G
Cuts at: 0 387 689 1203 1335
Size: 387 302 514 132
Fragments arranged by size:
514 387 302 132
PvuII CAG'CTG
Cuts at: 0 1009 1335
Size: 1009 326
SacI G_AGCT'C
Cuts at: 0 356 1139 1335
Size: 356 783 196
Fragments arranged by size:
783 356 196
SalI G'TCGA_C
Cuts at: 0 189 1335
Size: 189 1146
SbfI CC_TGCA'GG
Cuts at: 0 689 1335
Size: 689 646
SfcI C'TryA_G
Cuts at: 0 383 685 1199 1335
Size: 383 302 514 136
Fragments arranged by size:
514 383 302 136
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 169 1335
Size: 169 1166
SfoI GGC'GCC
Cuts at: 0 234 1335
Size: 234 1101
SmlI C'TyrA_G
Cuts at: 0 853 1056 1335
Size: 853 203 279
Fragments arranged by size:
853 279 203
StuI AGG'CCT
Cuts at: 0 601 1335
Size: 601 734
StyI C'CwwG_G
Cuts at: 0 596 1099 1205 1335
Size: 596 503 106 130
Fragments arranged by size:
596 503 130 106
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1165 1335
Size: 1165 170
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 300 332 350 382 1335
Size: 300 32 18 32 953
Fragments arranged by size:
953 300 32 32 18
Tth111I GACn'n_nGTC
Cuts at: 0 712 1335
Size: 712 623
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 270 1335
Size: 270 1065
Enzymes that do cut:
AccI AcuI AflII AflIII AhdI AlfI AlwNI ApoI
AvaI BanI BanII BbeI BciVI BglI BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspHI BsrBI BsrFI BssSI BstAPI BstYI BtgI
BtsI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI HaeII Hin4I HincII HindIII
KasI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
PasI PfoI PmlI PpuMI PstI PvuII SacI SalI
SbfI SfcI SfiI SfoI SmlI StuI StyI TaqII
TstI Tth111I XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AgeI AleI AloI
ApaI ApaLI AscI AseI AsiSI AvrII BaeI BamHI
BbsI BbvCI BcgI BclI BfrBI BglII BlpI Bme1580I
BmtI BsaI BsiWI BspEI BspMI BsrDI BsrGI BssHII
BstBI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI CspCI
DraI DraIII DrdI FseI FspI FspAI HpaI KpnI
MfeI MluI MmeI NdeI NheI NotI NruI NsiI
NspI PacI PciI PflMI PmeI PpiI PshAI PsiI
PspOMI PspXI PsrI PvuI RsrII SacII SanDI SapI
ScaI SexAI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI SwaI TatI XbaI XhoI XmaI XmnI
ZraI