Restriction map for
pF1KB3373
(Linear) (Six-base) MAPSORT of: KIBB3373.seq Check: 9541 from: 1 to: 1857
KIBB3373.orf
With 182 enzymes: SgfI *
July 18, 2007 13:36 ..
AccI GT'mk_AC
Cuts at: 0 63 1109 1857
Size: 63 1046 748
Fragments arranged by size:
1046 748 63
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 154 1507 1610 1799 1857
Size: 154 1353 103 189 58
Fragments arranged by size:
1353 189 154 103 58
AflII C'TTAA_G
Cuts at: 0 921 1830 1857
Size: 921 909 27
Fragments arranged by size:
921 909 27
AflIII A'CryG_T
Cuts at: 0 1838 1857
Size: 1838 19
AhdI GACnn_n'nnGTC
Cuts at: 0 1532 1857
Size: 1532 325
AleI CACnn'nnGTG
Cuts at: 0 1209 1857
Size: 1209 648
AlwNI CAG_nnn'CTG
Cuts at: 0 1227 1401 1857
Size: 1227 174 456
Fragments arranged by size:
1227 456 174
ApoI r'AATT_y
Cuts at: 0 139 630 736 769 1217 1687 1857
Size: 139 491 106 33 448 470 170
Fragments arranged by size:
491 470 448 170 139 106 33
AvaI C'yCGr_G
Cuts at: 0 1049 1769 1857
Size: 1049 720 88
Fragments arranged by size:
1049 720 88
AvrII C'CTAG_G
Cuts at: 0 703 1857
Size: 703 1154
BamHI G'GATC_C
Cuts at: 0 603 1278 1857
Size: 603 675 579
Fragments arranged by size:
675 603 579
BanII G_rGCy'C
Cuts at: 0 711 1056 1588 1857
Size: 711 345 532 269
Fragments arranged by size:
711 532 345 269
BclI T'GATC_A
Cuts at: 0 6 912 1857
Size: 6 906 945
Fragments arranged by size:
945 906 6
BglII A'GATC_T
Cuts at: 0 198 205 1857
Size: 198 7 1652
Fragments arranged by size:
1652 198 7
Bme1580I G_kGCm'C
Cuts at: 0 1570 1857
Size: 1570 287
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1795 1857
Size: 1795 62
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1086 1423 1653 1857
Size: 1086 337 230 204
Fragments arranged by size:
1086 337 230 204
Bpu10I CC'TnA_GC
Cuts at: 0 639 1857
Size: 639 1218
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 269 328 1228 1857
Size: 269 59 900 629
Fragments arranged by size:
900 629 269 59
BsaI GGTCTCn'nnnn_
Cuts at: 0 893 1177 1803 1857
Size: 893 284 626 54
Fragments arranged by size:
893 626 284 54
BsaBI GATnn'nnATC
Cuts at: 0 728 1857
Size: 728 1129
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 167 482 1857
Size: 167 315 1375
Fragments arranged by size:
1375 315 167
BseYI C'CCAG_C
Cuts at: 0 1036 1857
Size: 1036 821
BsiHKAI G_wGCw'C
Cuts at: 0 1056 1857
Size: 1056 801
BsmI GAATG_Cn'
Cuts at: 0 704 1857
Size: 704 1153
Bsp1286I G_dGCh'C
Cuts at: 0 711 1056 1570 1588 1857
Size: 711 345 514 18 269
Fragments arranged by size:
711 514 345 269 18
BsrDI GCAATG_nn'
Cuts at: 0 394 1703 1857
Size: 394 1309 154
Fragments arranged by size:
1309 394 154
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1702 1857
Size: 1702 155
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1308 1857
Size: 1308 549
BstYI r'GATC_y
Cuts at: 0 198 205 240 603 1278 1857
Size: 198 7 35 363 675 579
Fragments arranged by size:
675 579 363 198 35 7
DraI TTT'AAA
Cuts at: 0 1713 1857
Size: 1713 144
EaeI y'GGCC_r
Cuts at: 0 1086 1857
Size: 1086 771
EarI CTCTTCn'nnn_
Cuts at: 0 331 1131 1857
Size: 331 800 726
Fragments arranged by size:
800 726 331
EcoICRI GAG'CTC
Cuts at: 0 1054 1857
Size: 1054 803
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 154 1086 1423 1507 1610 1653 1799 1857
Size: 154 932 337 84 103 43 146 58
Fragments arranged by size:
932 337 154 146 103 84 58 43
EcoNI CCTnn'n_nnAGG
Cuts at: 0 52 497 1101 1857
Size: 52 445 604 756
Fragments arranged by size:
756 604 445 52
EcoO109I rG'GnC_Cy
Cuts at: 0 466 866 1857
Size: 466 400 991
Fragments arranged by size:
991 466 400
EcoRV GAT'ATC
Cuts at: 0 1449 1857
Size: 1449 408
HaeII r_GCGC'y
Cuts at: 0 1361 1857
Size: 1361 496
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 190 222 595 627 888 920 1518 1550
Size: 190 32 373 32 261 32 598 32
Cuts at: 1550 1857
Size: 307
Fragments arranged by size:
598 373 307 261 190 32 32 32
32
HincII GTy'rAC
Cuts at: 0 64 1857
Size: 64 1793
HindIII A'AGCT_T
Cuts at: 0 14 1857
Size: 14 1843
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1081 1857
Size: 1081 776
MscI TGG'CCA
Cuts at: 0 1088 1857
Size: 1088 769
MslI CAynn'nnrTG
Cuts at: 0 1209 1306 1843 1857
Size: 1209 97 537 14
Fragments arranged by size:
1209 537 97 14
MspA1I CmG'CkG
Cuts at: 0 1819 1857
Size: 1819 38
NdeI CA'TA_TG
Cuts at: 0 332 1353 1857
Size: 332 1021 504
Fragments arranged by size:
1021 504 332
NspI r_CATG'y
Cuts at: 0 1646 1842 1857
Size: 1646 196 15
Fragments arranged by size:
1646 196 15
PasI CC'CwG_GG
Cuts at: 0 870 1857
Size: 870 987
PciI A'CATG_T
Cuts at: 0 1838 1857
Size: 1838 19
PflMI CCAn_nnn'nTGG
Cuts at: 0 600 1857
Size: 600 1257
PpuMI rG'GwC_Cy
Cuts at: 0 466 866 1857
Size: 466 400 991
Fragments arranged by size:
991 466 400
PspXI vC'TCGA_Gb
Cuts at: 0 1049 1857
Size: 1049 808
PvuII CAG'CTG
Cuts at: 0 1819 1857
Size: 1819 38
SacI G_AGCT'C
Cuts at: 0 1056 1857
Size: 1056 801
SalI G'TCGA_C
Cuts at: 0 62 1857
Size: 62 1795
SapI GCTCTTCn'nnn_
Cuts at: 0 331 1857
Size: 331 1526
SexAI A'CCwGG_T
Cuts at: 0 524 1857
Size: 524 1333
SmlI C'TyrA_G
Cuts at: 0 284 343 921 1049 1207 1769 1830 1857
Size: 284 59 578 128 158 562 61 27
Fragments arranged by size:
578 562 284 158 128 61 59 27
SpeI A'CTAG_T
Cuts at: 0 472 550 928 1857
Size: 472 78 378 929
Fragments arranged by size:
929 472 378 78
StyI C'CwwG_G
Cuts at: 0 703 1089 1678 1857
Size: 703 386 589 179
Fragments arranged by size:
703 589 386 179
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1050 1857
Size: 1050 807
TatI w'GTAC_w
Cuts at: 0 370 1645 1857
Size: 370 1275 212
Fragments arranged by size:
1275 370 212
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 261 1068 1096 1857
Size: 261 807 28 761
Fragments arranged by size:
807 761 261 28
XhoI C'TCGA_G
Cuts at: 0 1049 1769 1857
Size: 1049 720 88
Fragments arranged by size:
1049 720 88
Enzymes that do cut:
AccI AcuI AflII AflIII AhdI AleI AlwNI ApoI
AvaI AvrII BamHI BanII BclI BglII Bme1580I BplI
BpmI Bpu10I BpuEI BsaI BsaBI BseRI BseYI BsiHKAI
BsmI Bsp1286I BsrDI BstAPI BstXI BstYI DraI EaeI
EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRV HaeII Hin4I
HincII HindIII MmeI MscI MslI MspA1I NdeI NspI
PasI PciI PflMI PpuMI PspXI PvuII SacI SalI
SapI SexAI SmlI SpeI StyI TaqII TatI XcmI
XhoI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AgeI AlfI AloI
ApaI ApaLI AscI AseI AsiSI BaeI BanI BbeI
BbsI BbvCI BcgI BciVI BfrBI BglI BlpI BmgBI
BmrI BmtI BsaAI BsaHI BsaWI BsaXI BsgI BsiEI
BsiWI BsmBI BspEI BspHI BspMI BsrBI BsrFI BsrGI
BssHII BssSI BstBI BstEII BstZ17I Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraIII DrdI EagI EciI EcoRI
FalI FseI FspI FspAI HpaI KasI KpnI MfeI
MluI NaeI NarI NcoI NgoMIV NheI NotI NruI
NsiI PacI PfoI PmeI PmlI PpiI PshAI PsiI
PspOMI PsrI PstI PvuI RsrII SacII SanDI SbfI
ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SnaBI
SphI SrfI SspI StuI SwaI TstI Tth111I XbaI
XmaI XmnI ZraI