Restriction map for
pF1KB3384
(Linear) (Six-base) MAPSORT of: KIBB3384.seq Check: 9161 from: 1 to: 2097
KIBB3384.orf
With 182 enzymes: SgfI *
October 14, 2008 16:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 748 2097
Size: 748 1349
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 758 1960 2097
Size: 758 1202 137
Fragments arranged by size:
1202 758 137
AflIII A'CryG_T
Cuts at: 0 513 1672 2097
Size: 513 1159 425
Fragments arranged by size:
1159 513 425
AlwNI CAG_nnn'CTG
Cuts at: 0 781 1045 1224 2097
Size: 781 264 179 873
Fragments arranged by size:
873 781 264 179
ApoI r'AATT_y
Cuts at: 0 1364 1897 2091 2097
Size: 1364 533 194 6
Fragments arranged by size:
1364 533 194 6
AvaI C'yCGr_G
Cuts at: 0 1369 1550 1892 2097
Size: 1369 181 342 205
Fragments arranged by size:
1369 342 205 181
BamHI G'GATC_C
Cuts at: 0 1411 1581 2097
Size: 1411 170 516
Fragments arranged by size:
1411 516 170
BanII G_rGCy'C
Cuts at: 0 7 2097
Size: 7 2090
BbsI GAAGACnn'nnnn_
Cuts at: 0 519 1256 1667 1808 1826 2097
Size: 519 737 411 141 18 271
Fragments arranged by size:
737 519 411 271 141 18
BbvCI CC'TCA_GC
Cuts at: 0 734 954 1034 1067 2058 2097
Size: 734 220 80 33 991 39
Fragments arranged by size:
991 734 220 80 39 33
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 510 544 2097
Size: 510 34 1553
Fragments arranged by size:
1553 510 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 503 2097
Size: 503 1594
BfrBI ATG'CAT
Cuts at: 0 712 2097
Size: 712 1385
BglI GCCn_nnn'nGGC
Cuts at: 0 28 2097
Size: 28 2069
Bme1580I G_kGCm'C
Cuts at: 0 305 378 2097
Size: 305 73 1719
Fragments arranged by size:
1719 305 73
BmrI ACTGGGnnnn_n'
Cuts at: 0 437 583 1535 2097
Size: 437 146 952 562
Fragments arranged by size:
952 562 437 146
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 812 2097
Size: 812 1285
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1234 1503 1755 1968 2097
Size: 1234 269 252 213 129
Fragments arranged by size:
1234 269 252 213 129
Bpu10I CC'TnA_GC
Cuts at: 0 734 954 1034 1067 2058 2097
Size: 734 220 80 33 991 39
Fragments arranged by size:
991 734 220 80 39 33
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 538 2097
Size: 538 1559
BsaI GGTCTCn'nnnn_
Cuts at: 0 248 2097
Size: 248 1849
BsaAI yAC'GTr
Cuts at: 0 1160 2097
Size: 1160 937
BsaBI GATnn'nnATC
Cuts at: 0 1130 2097
Size: 1130 967
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 95 98 184 187 211 541 755 758
Size: 95 3 86 3 24 330 214 3
Cuts at: 758 1039 1489 1928 2097
Size: 281 450 439 169
Fragments arranged by size:
450 439 330 281 214 169 95 86
24 3 3 3
BseYI C'CCAG_C
Cuts at: 0 326 377 2097
Size: 326 51 1720
Fragments arranged by size:
1720 326 51
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 944 1985 2097
Size: 944 1041 112
Fragments arranged by size:
1041 944 112
BsiHKAI G_wGCw'C
Cuts at: 0 7 765 1741 2097
Size: 7 758 976 356
Fragments arranged by size:
976 758 356 7
BsmI GAATG_Cn'
Cuts at: 0 1596 2074 2097
Size: 1596 478 23
Fragments arranged by size:
1596 478 23
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1808 2097
Size: 1808 289
Bsp1286I G_dGCh'C
Cuts at: 0 7 305 378 765 1741 2097
Size: 7 298 73 387 976 356
Fragments arranged by size:
976 387 356 298 73 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 748 2097
Size: 748 1349
BsrBI CCG'CTC
Cuts at: 0 1685 1708 2097
Size: 1685 23 389
Fragments arranged by size:
1685 389 23
BsrDI GCAATG_nn'
Cuts at: 0 362 2097
Size: 362 1735
BsrFI r'CCGG_y
Cuts at: 0 269 2097
Size: 269 1828
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1045 2097
Size: 1045 1052
BstBI TT'CG_AA
Cuts at: 0 530 2097
Size: 530 1567
BstYI r'GATC_y
Cuts at: 0 176 1411 1581 2097
Size: 176 1235 170 516
Fragments arranged by size:
1235 516 176 170
Bsu36I CC'TnA_GG
Cuts at: 0 1265 2097
Size: 1265 832
BtgI C'CryG_G
Cuts at: 0 1585 2097
Size: 1585 512
BtsI GCAGTG_nn'
Cuts at: 0 791 2097
Size: 791 1306
DraIII CAC_nnn'GTG
Cuts at: 0 746 1745 2097
Size: 746 999 352
Fragments arranged by size:
999 746 352
DrdI GACnn_nn'nnGTC
Cuts at: 0 466 2097
Size: 466 1631
EaeI y'GGCC_r
Cuts at: 0 20 267 540 1185 2097
Size: 20 247 273 645 912
Fragments arranged by size:
912 645 273 247 20
EarI CTCTTCn'nnn_
Cuts at: 0 265 598 856 1073 2097
Size: 265 333 258 217 1024
Fragments arranged by size:
1024 333 265 258 217
EcoICRI GAG'CTC
Cuts at: 0 5 2097
Size: 5 2092
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 758 1234 1503 1755 1960 1968 2097
Size: 758 476 269 252 205 8 129
Fragments arranged by size:
758 476 269 252 205 129 8
EcoNI CCTnn'n_nnAGG
Cuts at: 0 773 2097
Size: 773 1324
EcoO109I rG'GnC_Cy
Cuts at: 0 207 621 2097
Size: 207 414 1476
Fragments arranged by size:
1476 414 207
EcoRI G'AATT_C
Cuts at: 0 1897 2091 2097
Size: 1897 194 6
Fragments arranged by size:
1897 194 6
EcoRV GAT'ATC
Cuts at: 0 235 2097
Size: 235 1862
HaeII r_GCGC'y
Cuts at: 0 891 2097
Size: 891 1206
HindIII A'AGCT_T
Cuts at: 0 289 2097
Size: 289 1808
MluI A'CGCG_T
Cuts at: 0 1672 2097
Size: 1672 425
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 596 2097
Size: 596 1501
MscI TGG'CCA
Cuts at: 0 22 542 1187 2097
Size: 22 520 645 910
Fragments arranged by size:
910 645 520 22
MslI CAynn'nnrTG
Cuts at: 0 1994 2097
Size: 1994 103
MspA1I CmG'CkG
Cuts at: 0 733 958 1558 1587 2062 2097
Size: 733 225 600 29 475 35
Fragments arranged by size:
733 600 475 225 35 29
NdeI CA'TA_TG
Cuts at: 0 714 2097
Size: 714 1383
NsiI A_TGCA'T
Cuts at: 0 714 2097
Size: 714 1383
NspI r_CATG'y
Cuts at: 0 517 2097
Size: 517 1580
PciI A'CATG_T
Cuts at: 0 513 2097
Size: 513 1584
PflMI CCAn_nnn'nTGG
Cuts at: 0 746 1795 2097
Size: 746 1049 302
Fragments arranged by size:
1049 746 302
PfoI T'CCnGG_A
Cuts at: 0 7 2097
Size: 7 2090
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1363 1395 2097
Size: 1363 32 702
Fragments arranged by size:
1363 702 32
PpuMI rG'GwC_Cy
Cuts at: 0 207 621 2097
Size: 207 414 1476
Fragments arranged by size:
1476 414 207
PstI C_TGCA'G
Cuts at: 0 786 896 2097
Size: 786 110 1201
Fragments arranged by size:
1201 786 110
PvuII CAG'CTG
Cuts at: 0 733 958 1558 2062 2097
Size: 733 225 600 504 35
Fragments arranged by size:
733 600 504 225 35
SacI G_AGCT'C
Cuts at: 0 7 2097
Size: 7 2090
SacII CC_GC'GG
Cuts at: 0 1588 2097
Size: 1588 509
SbfI CC_TGCA'GG
Cuts at: 0 896 2097
Size: 896 1201
ScaI AGT'ACT
Cuts at: 0 536 1524 2097
Size: 536 988 573
Fragments arranged by size:
988 573 536
SfcI C'TryA_G
Cuts at: 0 782 812 892 2097
Size: 782 30 80 1205
Fragments arranged by size:
1205 782 80 30
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 28 2097
Size: 28 2069
SmaI CCC'GGG
Cuts at: 0 1552 2097
Size: 1552 545
SmlI C'TyrA_G
Cuts at: 0 553 2097
Size: 553 1544
SspI AAT'ATT
Cuts at: 0 1888 2097
Size: 1888 209
StuI AGG'CCT
Cuts at: 0 584 2097
Size: 584 1513
StyI C'CwwG_G
Cuts at: 0 398 1861 2083 2097
Size: 398 1463 222 14
Fragments arranged by size:
1463 398 222 14
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1629 2097
Size: 1629 468
TatI w'GTAC_w
Cuts at: 0 534 1522 1962 2097
Size: 534 988 440 135
Fragments arranged by size:
988 534 440 135
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1195 2097
Size: 1195 902
XmaI C'CCGG_G
Cuts at: 0 1550 2097
Size: 1550 547
XmnI GAAnn'nnTTC
Cuts at: 0 1509 1887 2039 2072 2097
Size: 1509 378 152 33 25
Fragments arranged by size:
1509 378 152 33 25
Enzymes that do cut:
AarI AcuI AflIII AlwNI ApoI AvaI BamHI BanII
BbsI BbvCI BcgI BciVI BfrBI BglI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BseRI
BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrBI
BsrDI BsrFI BstAPI BstBI BstYI Bsu36I BtgI BtsI
DraIII DrdI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV HaeII HindIII MluI MmeI MscI MslI
MspA1I NdeI NsiI NspI PciI PflMI PfoI PpiI
PpuMI PstI PvuII SacI SacII SbfI ScaI SfcI
SfiI SmaI SmlI SspI StuI StyI TaqII TatI
XcmI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI
AvrII BaeI BanI BbeI BclI BglII BlpI BmgBI
BmtI BsaHI BsaWI BsaXI BsiEI BsiWI BspEI BspHI
BsrGI BssHII BssSI BstEII BstXI BstZ17I BtgZI ClaI
CspCI DraI EagI EciI FalI FseI FspI FspAI
Hin4I HincII HpaI KasI KpnI MfeI NaeI NarI
NcoI NgoMIV NheI NotI NruI PacI PasI PmeI
PmlI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII
SalI SanDI SapI SexAI SfoI SgfI SgrAI SnaBI
SpeI SphI SrfI SwaI TstI Tth111I XbaI XhoI
ZraI