(Linear) (Six-base) MAPSORT of: KIBB3385.seq  Check: 5324  from: 1  to: 564



KIBB3385.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:56  ..

Acc65I G'GTAC_C

Cuts at:      0     282     564 
   Size:        282     282
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     313     564 
   Size:        313     251
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     427     564 
   Size:        427     137
 
ApaI G_GGCC'C

Cuts at:      0     327     564 
   Size:        327     237
 
ApaLI G'TGCA_C

Cuts at:      0     302     564 
   Size:        302     262
 
BanI G'GyrC_C

Cuts at:      0     282     564 
   Size:        282     282
 
BanII G_rGCy'C

Cuts at:      0      53     327     564 
   Size:         53     274     237

  Fragments arranged by size: 

                 274     237      53
 
Bme1580I G_kGCm'C

Cuts at:      0     306     327     564 
   Size:        306      21     237

  Fragments arranged by size: 

                 306     237      21
 
BsaWI w'CCGG_w

Cuts at:      0     200     418     564 
   Size:        200     218     146

  Fragments arranged by size: 

                 218     200     146
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     487     517     564 
   Size:        487      30      47

  Fragments arranged by size: 

                 487      47      30
 
BsiEI CG_ry'CG

Cuts at:      0     175     239     564 
   Size:        175      64     325

  Fragments arranged by size: 

                 325     175      64
 
BsiHKAI G_wGCw'C

Cuts at:      0     306     564 
   Size:        306     258
 
Bsp1286I G_dGCh'C

Cuts at:      0      53     306     327     564 
   Size:         53     253      21     237

  Fragments arranged by size: 

                 253     237      53      21
 
BspEI T'CCGG_A

Cuts at:      0     200     564 
   Size:        200     364
 
BsrBI CCG'CTC

Cuts at:      0     503     564 
   Size:        503      61
 
BsrFI r'CCGG_y

Cuts at:      0     170     446     564 
   Size:        170     276     118

  Fragments arranged by size: 

                 276     170     118
 
BstYI r'GATC_y

Cuts at:      0     356     564 
   Size:        356     208
 
Bsu36I CC'TnA_GG

Cuts at:      0      73     340     392     564 
   Size:         73     267      52     172

  Fragments arranged by size: 

                 267     172      73      52
 
BtgI C'CryG_G

Cuts at:      0     239     564 
   Size:        239     325
 
BtsI GCAGTG_nn'

Cuts at:      0     304     564 
   Size:        304     260
 
EaeI y'GGCC_r

Cuts at:      0     104     172     219     236     564 
   Size:        104      68      47      17     328

  Fragments arranged by size: 

                 328     104      68      47      17
 
EagI C'GGCC_G

Cuts at:      0     172     236     564 
   Size:        172      64     328

  Fragments arranged by size: 

                 328     172      64
 
EarI CTCTTCn'nnn_

Cuts at:      0     139     564 
   Size:        139     425
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0       4     564 
   Size:          4     560
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     313     564 
   Size:        313     251
 
EcoO109I rG'GnC_Cy

Cuts at:      0     462     564 
   Size:        462     102
 
EcoRV GAT'ATC

Cuts at:      0      66     564 
   Size:         66     498
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      28     564 
   Size:         28     536
 
HindIII A'AGCT_T

Cuts at:      0     211     564 
   Size:        211     353
 
KpnI G_GTAC'C

Cuts at:      0     286     564 
   Size:        286     278
 
MscI TGG'CCA

Cuts at:      0     106     564 
   Size:        106     458
 
MspA1I CmG'CkG

Cuts at:      0     375     564 
   Size:        375     189
 
PfoI T'CCnGG_A

Cuts at:      0     190     564 
   Size:        190     374
 
PspOMI G'GGCC_C

Cuts at:      0     323     564 
   Size:        323     241
 
PvuII CAG'CTG

Cuts at:      0     375     564 
   Size:        375     189
 
SexAI A'CCwGG_T

Cuts at:      0     131     564 
   Size:        131     433
 
StyI C'CwwG_G

Cuts at:      0     506     564 
   Size:        506      58
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      44     564 
   Size:         44     520
 

 Enzymes that do cut:

   Acc65I     AcuI     AlfI     ApaI    ApaLI     BanI    BanII Bme1580I
    BsaWI    BsaXI    BsiEI  BsiHKAI Bsp1286I    BspEI    BsrBI    BsrFI
    BstYI   Bsu36I     BtgI     BtsI     EaeI     EagI     EarI     EciI
  Eco57MI EcoO109I    EcoRV    Hin4I  HindIII     KpnI     MscI   MspA1I
     PfoI   PspOMI    PvuII    SexAI     StyI    TaqII

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AloI    AlwNI     ApoI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BbeI     BbsI    BbvCI     BcgI
    BciVI     BclI    BfrBI     BglI    BglII     BlpI    BmgBI     BmrI
     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BseRI    BseYI     BsgI    BsiWI     BsmI    BsmBI    BspHI
    BspMI    BsrDI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI  BstZ17I    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI
  EcoICRI    EcoNI    EcoRI     FalI     FseI     FspI    FspAI    HaeII
   HincII     HpaI     KasI     MfeI     MluI     MmeI     MslI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI    PflMI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI    PspXI     PsrI     PstI     PvuI    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI     SfcI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI     TatI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI