(Linear) (Six-base) MAPSORT of: KIBB3387.seq  Check: 1304  from: 1  to: 270



KIBB3387.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:56  ..

Acc65I G'GTAC_C

Cuts at:      0     153     270 
   Size:        153     117
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      38     269     270 
   Size:         38     231       1

  Fragments arranged by size: 

                 231      38       1
 
BanI G'GyrC_C

Cuts at:      0     153     270 
   Size:        153     117
 
BanII G_rGCy'C

Cuts at:      0     116     270 
   Size:        116     154
 
Bpu10I CC'TnA_GC

Cuts at:      0      29     270 
   Size:         29     241
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     137     167     270 
   Size:        137      30     103

  Fragments arranged by size: 

                 137     103      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      89     270 
   Size:         89     181
 
Bsp1286I G_dGCh'C

Cuts at:      0     116     270 
   Size:        116     154
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     102     270 
   Size:        102     168
 
BstYI r'GATC_y

Cuts at:      0      70     270 
   Size:         70     200
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      38     269     270 
   Size:         38     231       1

  Fragments arranged by size: 

                 231      38       1
 
EcoO109I rG'GnC_Cy

Cuts at:      0     129     270 
   Size:        129     141
 
KpnI G_GTAC'C

Cuts at:      0     157     270 
   Size:        157     113
 
PstI C_TGCA'G

Cuts at:      0     210     270 
   Size:        210      60
 
SfcI C'TryA_G

Cuts at:      0     206     270 
   Size:        206      64
 

 Enzymes that do cut:

   Acc65I     AcuI     BanI    BanII   Bpu10I    BsaXI    BseRI Bsp1286I
    BspMI    BstYI  Eco57MI EcoO109I     KpnI     PstI     SfcI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BbeI
     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BseYI     BsgI    BsiEI
  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI    EcoNI
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I
   HincII  HindIII     HpaI     KasI     MfeI     MluI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     StyI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     XmnI     ZraI