(Linear) (Six-base) MAPSORT of: KIBB3391.seq  Check: 9048  from: 1  to: 864



KIBB3391.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:56  ..

Acc65I G'GTAC_C

Cuts at:      0     307     864 
   Size:        307     557
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     374     381     514     864 
   Size:        374       7     133     350

  Fragments arranged by size: 

                 374     350     133       7
 
AhdI GACnn_n'nnGTC

Cuts at:      0     535     864 
   Size:        535     329
 
ApoI r'AATT_y

Cuts at:      0     574     767     839     864 
   Size:        574     193      72      25

  Fragments arranged by size: 

                 574     193      72      25
 
AvaI C'yCGr_G

Cuts at:      0     311     436     864 
   Size:        311     125     428

  Fragments arranged by size: 

                 428     311     125
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     291     299     324     332     864 
   Size:        291       8      25       8     532

  Fragments arranged by size: 

                 532     291      25       8       8
 
BanI G'GyrC_C

Cuts at:      0     307     864 
   Size:        307     557
 
BbvCI CC'TCA_GC

Cuts at:      0     794     864 
   Size:        794      70
 
BclI T'GATC_A

Cuts at:      0     449     744     864 
   Size:        449     295     120

  Fragments arranged by size: 

                 449     295     120
 
BglI GCCn_nnn'nGGC

Cuts at:      0     473     864 
   Size:        473     391
 
BglII A'GATC_T

Cuts at:      0     692     864 
   Size:        692     172
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     784     864 
   Size:        784      80
 
Bpu10I CC'TnA_GC

Cuts at:      0     794     864 
   Size:        794      70
 
BsaAI yAC'GTr

Cuts at:      0     408     864 
   Size:        408     456
 
BsaHI Gr'CG_yC

Cuts at:      0     465     864 
   Size:        465     399
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     155     818     864 
   Size:        155     663      46

  Fragments arranged by size: 

                 663     155      46
 
BsrDI GCAATG_nn'

Cuts at:      0     126     581     618     864 
   Size:        126     455      37     246

  Fragments arranged by size: 

                 455     246     126      37
 
BsrGI T'GTAC_A

Cuts at:      0     203     864 
   Size:        203     661
 
BstEII G'GTnAC_C

Cuts at:      0      88     864 
   Size:         88     776
 
BstYI r'GATC_y

Cuts at:      0     692     864 
   Size:        692     172
 
BtgI C'CryG_G

Cuts at:      0     381     864 
   Size:        381     483
 
ClaI AT'CG_AT

Cuts at:      0     602     864 
   Size:        602     262
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     374     381     514     784     864 
   Size:        374       7     133     270      80

  Fragments arranged by size: 

                 374     270     133      80       7
 
EcoRV GAT'ATC

Cuts at:      0     606     864 
   Size:        606     258
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     516     521     548     553     864 
   Size:        516       5      27       5     311

  Fragments arranged by size: 

                 516     311      27       5       5
 
KpnI G_GTAC'C

Cuts at:      0     311     864 
   Size:        311     553
 
MspA1I CmG'CkG

Cuts at:      0     501     864 
   Size:        501     363
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     289     864 
   Size:        289     575
 
PmlI CAC'GTG

Cuts at:      0     408     864 
   Size:        408     456
 
PvuII CAG'CTG

Cuts at:      0     501     864 
   Size:        501     363
 
ScaI AGT'ACT

Cuts at:      0     217     864 
   Size:        217     647
 
SmaI CCC'GGG

Cuts at:      0     313     438     864 
   Size:        313     125     426

  Fragments arranged by size: 

                 426     313     125
 
StyI C'CwwG_G

Cuts at:      0     233     864 
   Size:        233     631
 
TatI w'GTAC_w

Cuts at:      0     203     215     864 
   Size:        203      12     649

  Fragments arranged by size: 

                 649     203      12
 
XmaI C'CCGG_G

Cuts at:      0     311     436     864 
   Size:        311     125     428

  Fragments arranged by size: 

                 428     311     125
 

 Enzymes that do cut:

   Acc65I     AcuI     AhdI     ApoI     AvaI     BaeI     BanI    BbvCI
     BclI     BglI    BglII     BpmI   Bpu10I    BsaAI    BsaHI    BspMI
    BsrDI    BsrGI   BstEII    BstYI     BtgI     ClaI  Eco57MI    EcoRV
    Hin4I     KpnI   MspA1I    PflMI     PmlI    PvuII     ScaI     SmaI
     StyI     TatI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI    AflII   AflIII     AgeI
     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI
    AsiSI    AvrII    BamHI    BanII     BbeI     BbsI     BcgI    BciVI
    BfrBI     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI    BpuEI
     BsaI    BsaBI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BsrBI
    BsrFI   BssHII    BssSI   BstAPI    BstBI    BstXI  BstZ17I   Bsu36I
    BtgZI     BtsI    CspCI     DraI   DraIII     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI     FalI     FseI
     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI     MfeI
     MluI     MmeI     MscI     MslI     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI     PfoI     PmeI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI    SexAI     SfcI     SfiI     SfoI     SgfI
    SgrAI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI    TaqII     TstI  Tth111I     XbaI     XcmI     XhoI     XmnI
     ZraI