(Linear) (Six-base) MAPSORT of: KIBB3399.seq  Check: 7707  from: 1  to: 558



KIBB3399.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:56  ..

AclI AA'CG_TT

Cuts at:      0     311     558 
   Size:        311     247
 
AflII C'TTAA_G

Cuts at:      0      81     558 
   Size:         81     477
 
ApaI G_GGCC'C

Cuts at:      0     485     558 
   Size:        485      73
 
ApoI r'AATT_y

Cuts at:      0     232     355     551     558 
   Size:        232     123     196       7

  Fragments arranged by size: 

                 232     196     123       7
 
AseI AT'TA_AT

Cuts at:      0     142     558 
   Size:        142     416
 
BanI G'GyrC_C

Cuts at:      0     238     558 
   Size:        238     320
 
BanII G_rGCy'C

Cuts at:      0     485     558 
   Size:        485      73
 
BlpI GC'TnA_GC

Cuts at:      0     249     558 
   Size:        249     309
 
Bme1580I G_kGCm'C

Cuts at:      0     485     558 
   Size:        485      73
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     227     558 
   Size:        227     331
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      95     125     558 
   Size:         95      30     433

  Fragments arranged by size: 

                 433      95      30
 
Bsp1286I G_dGCh'C

Cuts at:      0     485     558 
   Size:        485      73
 
Bsu36I CC'TnA_GG

Cuts at:      0     186     558 
   Size:        186     372
 
BtsI GCAGTG_nn'

Cuts at:      0     326     558 
   Size:        326     232
 
DraI TTT'AAA

Cuts at:      0      77     558 
   Size:         77     481
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     227     558 
   Size:        227     331
 
EcoO109I rG'GnC_Cy

Cuts at:      0     481     558 
   Size:        481      77
 
EcoRI G'AATT_C

Cuts at:      0     355     558 
   Size:        355     203
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     414     446     558 
   Size:        414      32     112

  Fragments arranged by size: 

                 414     112      32
 
HincII GTy'rAC

Cuts at:      0     309     558 
   Size:        309     249
 
HindIII A'AGCT_T

Cuts at:      0     148     558 
   Size:        148     410
 
HpaI GTT'AAC

Cuts at:      0     309     558 
   Size:        309     249
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     111     558 
   Size:        111     447
 
NspI r_CATG'y

Cuts at:      0     333     558 
   Size:        333     225
 
PsiI TTA'TAA

Cuts at:      0     119     558 
   Size:        119     439
 
PspOMI G'GGCC_C

Cuts at:      0     481     558 
   Size:        481      77
 
PstI C_TGCA'G

Cuts at:      0     266     558 
   Size:        266     292
 
SfcI C'TryA_G

Cuts at:      0     262     558 
   Size:        262     296
 
SmlI C'TyrA_G

Cuts at:      0      81     558 
   Size:         81     477
 
SspI AAT'ATT

Cuts at:      0     287     558 
   Size:        287     271
 

 Enzymes that do cut:

     AclI    AflII     ApaI     ApoI     AseI     BanI    BanII     BlpI
 Bme1580I     BpmI    BsaXI Bsp1286I   Bsu36I     BtsI     DraI  Eco57MI
 EcoO109I    EcoRI    Hin4I   HincII  HindIII     HpaI     MmeI     NspI
     PsiI   PspOMI     PstI     SfcI     SmlI     SspI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AcuI     AfeI   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI    ApaLI     AscI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BbeI     BbsI    BbvCI     BcgI
    BciVI     BclI    BfrBI     BglI    BglII    BmgBI     BmrI     BmtI
     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I     BtgI
    BtgZI     ClaI    CspCI   DraIII     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI    EcoNI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII     KasI     KpnI     MfeI     MluI     MscI     MslI   MspA1I
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI    PspXI     PsrI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI
     SrfI     StuI     StyI     SwaI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI