(Linear) (Six-base) MAPSORT of: KIBB3400.seq Check: 9415 from: 1 to: 315 KIBB3400.orf With 182 enzymes: SgfI * January 9, 2009 11:10 .. BbsI GAAGACnn'nnnn_ Cuts at: 0 124 315 Size: 124 191 BciVI GTATCCnnnnn_n' Cuts at: 0 130 180 315 Size: 130 50 135 Fragments arranged by size: 135 130 50 BmrI ACTGGGnnnn_n' Cuts at: 0 42 95 315 Size: 42 53 220 Fragments arranged by size: 220 53 42 BsaI GGTCTCn'nnnn_ Cuts at: 0 109 315 Size: 109 206 BtgI C'CryG_G Cuts at: 0 99 315 Size: 99 216 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 129 315 Size: 129 186 EcoO109I rG'GnC_Cy Cuts at: 0 129 315 Size: 129 186 NcoI C'CATG_G Cuts at: 0 99 315 Size: 99 216 StyI C'CwwG_G Cuts at: 0 99 315 Size: 99 216 TatI w'GTAC_w Cuts at: 0 221 315 Size: 221 94 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 106 315 Size: 106 209 Enzymes that do cut: BbsI BciVI BmrI BsaI BtgI EciI EcoO109I NcoI StyI TatI XcmI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI BbvCI BcgI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmtI BplI BpmI Bpu10I BpuEI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EcoICRI Eco57MI EcoNI EcoRI EcoRV FalI FseI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII TstI Tth111I XbaI XhoI XmaI XmnI ZraI