Restriction map for
pF1KB3401
(Linear) (Six-base) MAPSORT of: KIBB3401.seq Check: 9946 from: 1 to: 747
KIBB3401.orf
With 182 enzymes: SgfI *
April 24, 2009 13:01 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 601 747
Size: 601 146
AflIII A'CryG_T
Cuts at: 0 546 747
Size: 546 201
AlwNI CAG_nnn'CTG
Cuts at: 0 28 747
Size: 28 719
ApaI G_GGCC'C
Cuts at: 0 403 747
Size: 403 344
AvrII C'CTAG_G
Cuts at: 0 505 747
Size: 505 242
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 368 401 747
Size: 368 33 346
Fragments arranged by size:
368 346 33
BanII G_rGCy'C
Cuts at: 0 403 747
Size: 403 344
BglI GCCn_nnn'nGGC
Cuts at: 0 398 747
Size: 398 349
Bme1580I G_kGCm'C
Cuts at: 0 403 747
Size: 403 344
BmtI G_CTAG'C
Cuts at: 0 416 747
Size: 416 331
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 287 747
Size: 287 460
Bpu10I CC'TnA_GC
Cuts at: 0 659 747
Size: 659 88
BsaAI yAC'GTr
Cuts at: 0 547 747
Size: 547 200
BsaBI GATnn'nnATC
Cuts at: 0 311 747
Size: 311 436
BseYI C'CCAG_C
Cuts at: 0 21 747
Size: 21 726
BsmI GAATG_Cn'
Cuts at: 0 240 747
Size: 240 507
BsmBI CGTCTCn'nnnn_
Cuts at: 0 684 747
Size: 684 63
Bsp1286I G_dGCh'C
Cuts at: 0 403 747
Size: 403 344
BstAPI GCAn_nnn'nTGC
Cuts at: 0 625 747
Size: 625 122
BtgI C'CryG_G
Cuts at: 0 207 747
Size: 207 540
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 87 122 747
Size: 87 35 625
Fragments arranged by size:
625 87 35
EaeI y'GGCC_r
Cuts at: 0 95 210 638 747
Size: 95 115 428 109
Fragments arranged by size:
428 115 109 95
EarI CTCTTCn'nnn_
Cuts at: 0 605 682 747
Size: 605 77 65
Fragments arranged by size:
605 77 65
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 208 448 747
Size: 208 240 299
Fragments arranged by size:
299 240 208
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 287 601 747
Size: 287 314 146
Fragments arranged by size:
314 287 146
EcoO109I rG'GnC_Cy
Cuts at: 0 400 747
Size: 400 347
HaeII r_GCGC'y
Cuts at: 0 672 747
Size: 672 75
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 516 548 747
Size: 516 32 199
Fragments arranged by size:
516 199 32
MscI TGG'CCA
Cuts at: 0 97 640 747
Size: 97 543 107
Fragments arranged by size:
543 107 97
MslI CAynn'nnrTG
Cuts at: 0 49 212 747
Size: 49 163 535
Fragments arranged by size:
535 163 49
NcoI C'CATG_G
Cuts at: 0 207 747
Size: 207 540
NheI G'CTAG_C
Cuts at: 0 412 747
Size: 412 335
PflMI CCAn_nnn'nTGG
Cuts at: 0 28 747
Size: 28 719
PfoI T'CCnGG_A
Cuts at: 0 363 747
Size: 363 384
PmlI CAC'GTG
Cuts at: 0 547 747
Size: 547 200
PspOMI G'GGCC_C
Cuts at: 0 399 747
Size: 399 348
PstI C_TGCA'G
Cuts at: 0 557 621 747
Size: 557 64 126
Fragments arranged by size:
557 126 64
SfcI C'TryA_G
Cuts at: 0 380 553 617 747
Size: 380 173 64 130
Fragments arranged by size:
380 173 130 64
StyI C'CwwG_G
Cuts at: 0 207 256 505 747
Size: 207 49 249 242
Fragments arranged by size:
249 242 207 49
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 112 534 747
Size: 112 422 213
Fragments arranged by size:
422 213 112
Enzymes that do cut:
AcuI AflIII AlwNI ApaI AvrII BaeI BanII BglI
Bme1580I BmtI BpmI Bpu10I BsaAI BsaBI BseYI BsmI
BsmBI Bsp1286I BstAPI BtgI CspCI EaeI EarI EciI
Eco57MI EcoO109I HaeII Hin4I MscI MslI NcoI NheI
PflMI PfoI PmlI PspOMI PstI SfcI StyI TaqII
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI AloI ApaLI ApoI AscI AseI
AsiSI AvaI BamHI BanI BbeI BbsI BbvCI BcgI
BciVI BclI BfrBI BglII BlpI BmgBI BmrI BplI
BpuEI BsaI BsaHI BsaWI BsaXI BseRI BsgI BsiEI
BsiHKAI BsiWI BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstBI BstEII BstXI BstYI BstZ17I
Bsu36I BtgZI BtsI ClaI DraI DraIII DrdI EagI
EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MspA1I NaeI NarI NdeI NgoMIV NotI NruI NsiI
NspI PacI PasI PciI PmeI PpiI PpuMI PshAI
PsiI PspXI PsrI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI SwaI TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI