Restriction map for
pF1KB3408
(Linear) (Six-base) MAPSORT of: KIBB3408.seq Check: 1004 from: 1 to: 2997
KIBB3408.orf
With 182 enzymes: SgfI *
February 28, 2007 11:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 72 2997
Size: 72 2925
AccI GT'mk_AC
Cuts at: 0 878 1165 2997
Size: 878 287 1832
Fragments arranged by size:
1832 878 287
Acc65I G'GTAC_C
Cuts at: 0 1156 2997
Size: 1156 1841
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 376 2152 2641 2997
Size: 376 1776 489 356
Fragments arranged by size:
1776 489 376 356
AfeI AGC'GCT
Cuts at: 0 103 1570 2997
Size: 103 1467 1427
Fragments arranged by size:
1467 1427 103
AflIII A'CryG_T
Cuts at: 0 1358 2997
Size: 1358 1639
AleI CACnn'nnGTG
Cuts at: 0 1894 2173 2997
Size: 1894 279 824
Fragments arranged by size:
1894 824 279
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 211 424 1768 2997
Size: 211 213 1344 1229
Fragments arranged by size:
1344 1229 213 211
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2059 2091 2997
Size: 2059 32 906
Fragments arranged by size:
2059 906 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1383 1635 2492 2548 2763 2997
Size: 1383 252 857 56 215 234
Fragments arranged by size:
1383 857 252 234 215 56
AvaI C'yCGr_G
Cuts at: 0 123 425 730 922 1152 1171 1388 1463
Size: 123 302 305 192 230 19 217 75
Cuts at: 1463 1697 1987 2471 2997
Size: 234 290 484 526
Fragments arranged by size:
526 484 305 302 290 234 230 217
192 123 75 19
BamHI G'GATC_C
Cuts at: 0 416 2997
Size: 416 2581
BanI G'GyrC_C
Cuts at: 0 631 1156 1405 2891 2997
Size: 631 525 249 1486 106
Fragments arranged by size:
1486 631 525 249 106
BanII G_rGCy'C
Cuts at: 0 76 122 272 731 1126 1551 1660 2042
Size: 76 46 150 459 395 425 109 382
Cuts at: 2042 2164 2470 2997
Size: 122 306 527
Fragments arranged by size:
527 459 425 395 382 306 150 122
109 76 46
BbeI G_GCGC'C
Cuts at: 0 1409 2895 2997
Size: 1409 1486 102
Fragments arranged by size:
1486 1409 102
BbsI GAAGACnn'nnnn_
Cuts at: 0 1943 2639 2997
Size: 1943 696 358
Fragments arranged by size:
1943 696 358
BbvCI CC'TCA_GC
Cuts at: 0 1981 2608 2997
Size: 1981 627 389
Fragments arranged by size:
1981 627 389
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1099 1133 2074 2108 2997
Size: 1099 34 941 34 889
Fragments arranged by size:
1099 941 889 34 34
BclI T'GATC_A
Cuts at: 0 1868 2997
Size: 1868 1129
BglI GCCn_nnn'nGGC
Cuts at: 0 349 844 2185 2545 2997
Size: 349 495 1341 360 452
Fragments arranged by size:
1341 495 452 360 349
Bme1580I G_kGCm'C
Cuts at: 0 426 967 1063 2997
Size: 426 541 96 1934
Fragments arranged by size:
1934 541 426 96
BmgBI CAC'GTC
Cuts at: 0 33 1941 2997
Size: 33 1908 1056
Fragments arranged by size:
1908 1056 33
BmrI ACTGGGnnnn_n'
Cuts at: 0 970 2997
Size: 970 2027
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2497 2997
Size: 2497 500
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1474 1605 2896 2997
Size: 1474 131 1291 101
Fragments arranged by size:
1474 1291 131 101
Bpu10I CC'TnA_GC
Cuts at: 0 70 1659 1981 2163 2608 2997
Size: 70 1589 322 182 445 389
Fragments arranged by size:
1589 445 389 322 182 70
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2935 2997
Size: 2935 62
BsaI GGTCTCn'nnnn_
Cuts at: 0 241 1012 1460 2997
Size: 241 771 448 1537
Fragments arranged by size:
1537 771 448 241
BsaAI yAC'GTr
Cuts at: 0 1163 2529 2997
Size: 1163 1366 468
Fragments arranged by size:
1366 1163 468
BsaBI GATnn'nnATC
Cuts at: 0 2149 2997
Size: 2149 848
BsaHI Gr'CG_yC
Cuts at: 0 739 1406 2576 2892 2997
Size: 739 667 1170 316 105
Fragments arranged by size:
1170 739 667 316 105
BsaWI w'CCGG_w
Cuts at: 0 704 1469 2997
Size: 704 765 1528
Fragments arranged by size:
1528 765 704
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 841 871 2206 2236 2278 2308 2513 2543
Size: 841 30 1335 30 42 30 205 30
Cuts at: 2543 2743 2773 2997
Size: 200 30 224
Fragments arranged by size:
1335 841 224 205 200 42 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 250 259 2257 2286 2997
Size: 250 9 1998 29 711
Fragments arranged by size:
1998 711 250 29 9
BseYI C'CCAG_C
Cuts at: 0 693 2997
Size: 693 2304
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 430 667 1014 1476 1774 2389 2812 2997
Size: 430 237 347 462 298 615 423 185
Fragments arranged by size:
615 462 430 423 347 298 237 185
BsiEI CG_ry'CG
Cuts at: 0 26 257 2174 2997
Size: 26 231 1917 823
Fragments arranged by size:
1917 823 231 26
BsiHKAI G_wGCw'C
Cuts at: 0 122 2629 2711 2912 2997
Size: 122 2507 82 201 85
Fragments arranged by size:
2507 201 122 85 82
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1756 2751 2997
Size: 1756 995 246
Fragments arranged by size:
1756 995 246
Bsp1286I G_dGCh'C
Cuts at: 0 76 122 272 426 731 967 1063 1126
Size: 76 46 150 154 305 236 96 63
Cuts at: 1126 1551 1660 2042 2164 2470 2629 2711 2912
Size: 425 109 382 122 306 159 82 201
Cuts at: 2912 2997
Size: 85
Fragments arranged by size:
425 382 306 305 236 201 159 154
150 122 109 96 85 82 76 63
46
BspEI T'CCGG_A
Cuts at: 0 704 2997
Size: 704 2293
BspHI T'CATG_A
Cuts at: 0 1865 2997
Size: 1865 1132
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 72 1382 2078 2415 2997
Size: 72 1310 696 337 582
Fragments arranged by size:
1310 696 582 337 72
BsrBI CCG'CTC
Cuts at: 0 392 735 2643 2888 2997
Size: 392 343 1908 245 109
Fragments arranged by size:
1908 392 343 245 109
BsrDI GCAATG_nn'
Cuts at: 0 2736 2997
Size: 2736 261
BsrFI r'CCGG_y
Cuts at: 0 88 676 2101 2650 2997
Size: 88 588 1425 549 347
Fragments arranged by size:
1425 588 549 347 88
BssHII G'CGCG_C
Cuts at: 0 92 2997
Size: 92 2905
BssSI C'ACGA_G
Cuts at: 0 292 1138 2710 2845 2997
Size: 292 846 1572 135 152
Fragments arranged by size:
1572 846 292 152 135
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1434 2997
Size: 1434 1563
BstEII G'GTnAC_C
Cuts at: 0 84 2874 2997
Size: 84 2790 123
Fragments arranged by size:
2790 123 84
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2418 2694 2820 2997
Size: 2418 276 126 177
Fragments arranged by size:
2418 276 177 126
BstYI r'GATC_y
Cuts at: 0 416 701 1631 1643 2997
Size: 416 285 930 12 1354
Fragments arranged by size:
1354 930 416 285 12
BstZ17I GTA'TAC
Cuts at: 0 1166 2997
Size: 1166 1831
Bsu36I CC'TnA_GG
Cuts at: 0 1814 2997
Size: 1814 1183
BtgI C'CryG_G
Cuts at: 0 668 849 896 1013 1319 1895 2156 2174
Size: 668 181 47 117 306 576 261 18
Cuts at: 2174 2411 2693 2819 2997
Size: 237 282 126 178
Fragments arranged by size:
668 576 306 282 261 237 181 178
126 117 47 18
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2120 2136 2997
Size: 2120 16 861
Fragments arranged by size:
2120 861 16
BtsI GCAGTG_nn'
Cuts at: 0 467 1889 2090 2997
Size: 467 1422 201 907
Fragments arranged by size:
1422 907 467 201
DraIII CAC_nnn'GTG
Cuts at: 0 2561 2997
Size: 2561 436
EaeI y'GGCC_r
Cuts at: 0 23 296 665 671 806 828 914 1212
Size: 23 273 369 6 135 22 86 298
Cuts at: 1212 1518 1691 1898 2171 2177 2537 2810 2997
Size: 306 173 207 273 6 360 273 187
Fragments arranged by size:
369 360 306 298 273 273 273 207
187 173 135 86 23 22 6 6
EagI C'GGCC_G
Cuts at: 0 23 2171 2997
Size: 23 2148 826
Fragments arranged by size:
2148 826 23
EarI CTCTTCn'nnn_
Cuts at: 0 2503 2997
Size: 2503 494
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 478 2997
Size: 478 2519
EcoICRI GAG'CTC
Cuts at: 0 120 2997
Size: 120 2877
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 376 1474 1605 2152 2641 2896 2997
Size: 376 1098 131 547 489 255 101
Fragments arranged by size:
1098 547 489 376 255 131 101
EcoO109I rG'GnC_Cy
Cuts at: 0 174 351 927 2584 2946 2997
Size: 174 177 576 1657 362 51
Fragments arranged by size:
1657 576 362 177 174 51
EcoRV GAT'ATC
Cuts at: 0 2918 2997
Size: 2918 79
FspI TGC'GCA
Cuts at: 0 187 2997
Size: 187 2810
FspAI rTGC'GCAy
Cuts at: 0 187 2997
Size: 187 2810
HaeII r_GCGC'y
Cuts at: 0 105 875 1409 1572 2001 2895 2997
Size: 105 770 534 163 429 894 102
Fragments arranged by size:
894 770 534 429 163 105 102
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 490 522 1108 1140 1459 1491 1642 1674
Size: 490 32 586 32 319 32 151 32
Cuts at: 1674 2576 2608 2743 2775 2997
Size: 902 32 135 32 222
Fragments arranged by size:
902 586 490 319 222 151 135 32
32 32 32 32 32
KasI G'GCGC_C
Cuts at: 0 1405 2891 2997
Size: 1405 1486 106
Fragments arranged by size:
1486 1405 106
KpnI G_GTAC'C
Cuts at: 0 1160 2997
Size: 1160 1837
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1073 2997
Size: 1073 1924
MscI TGG'CCA
Cuts at: 0 298 673 1520 1693 1900 2179 2539 2812
Size: 298 375 847 173 207 279 360 273
Cuts at: 2812 2997
Size: 185
Fragments arranged by size:
847 375 360 298 279 273 207 185
173
MslI CAynn'nnrTG
Cuts at: 0 793 1483 1858 1894 2095 2155 2173 2692
Size: 793 690 375 36 201 60 18 519
Cuts at: 2692 2722 2818 2997
Size: 30 96 179
Fragments arranged by size:
793 690 519 375 201 179 96 60
36 30 18
MspA1I CmG'CkG
Cuts at: 0 744 777 1431 2021 2373 2607 2997
Size: 744 33 654 590 352 234 390
Fragments arranged by size:
744 654 590 390 352 234 33
NaeI GCC'GGC
Cuts at: 0 2652 2997
Size: 2652 345
NarI GG'CG_CC
Cuts at: 0 1406 2892 2997
Size: 1406 1486 105
Fragments arranged by size:
1486 1406 105
NcoI C'CATG_G
Cuts at: 0 2156 2693 2819 2997
Size: 2156 537 126 178
Fragments arranged by size:
2156 537 178 126
NgoMIV G'CCGG_C
Cuts at: 0 2650 2997
Size: 2650 347
NspI r_CATG'y
Cuts at: 0 621 953 1797 2976 2997
Size: 621 332 844 1179 21
Fragments arranged by size:
1179 844 621 332 21
PasI CC'CwG_GG
Cuts at: 0 439 1023 2997
Size: 439 584 1974
Fragments arranged by size:
1974 584 439
PflMI CCAn_nnn'nTGG
Cuts at: 0 2561 2901 2997
Size: 2561 340 96
Fragments arranged by size:
2561 340 96
PfoI T'CCnGG_A
Cuts at: 0 1646 2962 2997
Size: 1646 1316 35
Fragments arranged by size:
1646 1316 35
PpuMI rG'GwC_Cy
Cuts at: 0 174 2584 2997
Size: 174 2410 413
Fragments arranged by size:
2410 413 174
PstI C_TGCA'G
Cuts at: 0 749 1379 1744 2075 2997
Size: 749 630 365 331 922
Fragments arranged by size:
922 749 630 365 331
PvuII CAG'CTG
Cuts at: 0 777 1431 2373 2607 2997
Size: 777 654 942 234 390
Fragments arranged by size:
942 777 654 390 234
RsrII CG'GwC_CG
Cuts at: 0 396 2997
Size: 396 2601
SacI G_AGCT'C
Cuts at: 0 122 2997
Size: 122 2875
SapI GCTCTTCn'nnn_
Cuts at: 0 2503 2997
Size: 2503 494
SbfI CC_TGCA'GG
Cuts at: 0 1379 2997
Size: 1379 1618
SexAI A'CCwGG_T
Cuts at: 0 2387 2557 2997
Size: 2387 170 440
Fragments arranged by size:
2387 440 170
SfcI C'TryA_G
Cuts at: 0 745 1375 1572 1740 2071 2259 2997
Size: 745 630 197 168 331 188 738
Fragments arranged by size:
745 738 630 331 197 188 168
SfoI GGC'GCC
Cuts at: 0 1407 2893 2997
Size: 1407 1486 104
Fragments arranged by size:
1486 1407 104
SmaI CCC'GGG
Cuts at: 0 427 1390 1699 2997
Size: 427 963 309 1298
Fragments arranged by size:
1298 963 427 309
SmlI C'TyrA_G
Cuts at: 0 2950 2997
Size: 2950 47
SnaBI TAC'GTA
Cuts at: 0 2529 2997
Size: 2529 468
SphI G_CATG'C
Cuts at: 0 1797 2997
Size: 1797 1200
StuI AGG'CCT
Cuts at: 0 1954 2005 2534 2997
Size: 1954 51 529 463
Fragments arranged by size:
1954 529 463 51
StyI C'CwwG_G
Cuts at: 0 1289 1711 2156 2693 2819 2997
Size: 1289 422 445 537 126 178
Fragments arranged by size:
1289 537 445 422 178 126
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 649 2370 2889 2997
Size: 649 1721 519 108
Fragments arranged by size:
1721 649 519 108
TatI w'GTAC_w
Cuts at: 0 1487 2997
Size: 1487 1510
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 841 873 2997
Size: 841 32 2124
Fragments arranged by size:
2124 841 32
Tth111I GACn'n_nGTC
Cuts at: 0 433 2997
Size: 433 2564
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 306 519 2997
Size: 306 213 2478
Fragments arranged by size:
2478 306 213
XmaI C'CCGG_G
Cuts at: 0 425 1388 1697 2997
Size: 425 963 309 1300
Fragments arranged by size:
1300 963 425 309
XmnI GAAnn'nnTTC
Cuts at: 0 1360 1444 2275 2997
Size: 1360 84 831 722
Fragments arranged by size:
1360 831 722 84
Enzymes that do cut:
AarI AccI Acc65I AcuI AfeI AflIII AleI AlfI
AloI AlwNI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BclI BglI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI
BstAPI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI DraIII EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRV FspI FspAI HaeII Hin4I KasI KpnI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PflMI PfoI PpuMI PstI PvuII RsrII
SacI SapI SbfI SexAI SfcI SfoI SmaI SmlI
SnaBI SphI StuI StyI TaqII TatI TstI Tth111I
XcmI XmaI XmnI
Enzymes that do not cut:
AatII AclI AflII AgeI AhdI ApaI ApaLI ApoI
AscI AseI AsiSI AvrII BaeI BciVI BfrBI BglII
BlpI BmtI BsiWI BsmI BsrGI BstBI ClaI CspCI
DraI DrdI EcoNI EcoRI FalI FseI HincII HindIII
HpaI MfeI MluI NdeI NheI NotI NruI NsiI
PacI PciI PmeI PmlI PpiI PshAI PsiI PspOMI
PspXI PsrI PvuI SacII SalI SanDI ScaI SfiI
SgfI SgrAI SpeI SrfI SspI SwaI XbaI XhoI
ZraI