Restriction map for
pF1KB3411
(Linear) (Six-base) MAPSORT of: KIBB3411.seq Check: 969 from: 1 to: 2697
KIBB3411.orf
With 182 enzymes: SgfI *
February 28, 2007 11:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1445 1583 2697
Size: 1445 138 1114
Fragments arranged by size:
1445 1114 138
AccI GT'mk_AC
Cuts at: 0 2411 2490 2697
Size: 2411 79 207
Fragments arranged by size:
2411 207 79
Acc65I G'GTAC_C
Cuts at: 0 2036 2697
Size: 2036 661
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 445 562 646 703 1729 1892 2083 2171
Size: 445 117 84 57 1026 163 191 88
Cuts at: 2171 2697
Size: 526
Fragments arranged by size:
1026 526 445 191 163 117 88 84
57
AflIII A'CryG_T
Cuts at: 0 947 2414 2697
Size: 947 1467 283
Fragments arranged by size:
1467 947 283
AleI CACnn'nnGTG
Cuts at: 0 838 1067 2221 2578 2697
Size: 838 229 1154 357 119
Fragments arranged by size:
1154 838 357 229 119
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2467 2697
Size: 2467 230
AlwNI CAG_nnn'CTG
Cuts at: 0 715 1714 2331 2697
Size: 715 999 617 366
Fragments arranged by size:
999 715 617 366
ApaI G_GGCC'C
Cuts at: 0 377 1180 2641 2697
Size: 377 803 1461 56
Fragments arranged by size:
1461 803 377 56
ApaLI G'TGCA_C
Cuts at: 0 1736 2692 2697
Size: 1736 956 5
Fragments arranged by size:
1736 956 5
AvaI C'yCGr_G
Cuts at: 0 1194 1328 1373 2555 2697
Size: 1194 134 45 1182 142
Fragments arranged by size:
1194 1182 142 134 45
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2020 2053 2697
Size: 2020 33 644
Fragments arranged by size:
2020 644 33
BanI G'GyrC_C
Cuts at: 0 199 1230 2036 2675 2697
Size: 199 1031 806 639 22
Fragments arranged by size:
1031 806 639 199 22
BanII G_rGCy'C
Cuts at: 0 377 1180 1329 1380 1758 1830 2102 2641
Size: 377 803 149 51 378 72 272 539
Cuts at: 2641 2666 2697
Size: 25 31
Fragments arranged by size:
803 539 378 377 272 149 72 51
31 25
BbsI GAAGACnn'nnnn_
Cuts at: 0 995 2233 2594 2697
Size: 995 1238 361 103
Fragments arranged by size:
1238 995 361 103
BbvCI CC'TCA_GC
Cuts at: 0 2539 2697
Size: 2539 158
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1309 1343 2697
Size: 1309 34 1354
Fragments arranged by size:
1354 1309 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1814 2697
Size: 1814 883
BclI T'GATC_A
Cuts at: 0 1604 2697
Size: 1604 1093
BfrBI ATG'CAT
Cuts at: 0 1692 2697
Size: 1692 1005
BglI GCCn_nnn'nGGC
Cuts at: 0 1184 1352 2697
Size: 1184 168 1345
Fragments arranged by size:
1345 1184 168
BglII A'GATC_T
Cuts at: 0 242 2697
Size: 242 2455
Bme1580I G_kGCm'C
Cuts at: 0 377 1180 1235 1740 1769 2110 2641 2678
Size: 377 803 55 505 29 341 531 37
Cuts at: 2678 2696 2697
Size: 18 1
Fragments arranged by size:
803 531 505 377 341 55 37 29
18 1
BmgBI CAC'GTC
Cuts at: 0 948 2697
Size: 948 1749
BmrI ACTGGGnnnn_n'
Cuts at: 0 1284 2041 2432 2697
Size: 1284 757 391 265
Fragments arranged by size:
1284 757 391 265
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1399 1772 2697
Size: 1399 373 925
Fragments arranged by size:
1399 925 373
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 189 541 1130 1263 2353 2515 2697
Size: 189 352 589 133 1090 162 182
Fragments arranged by size:
1090 589 352 189 182 162 133
Bpu10I CC'TnA_GC
Cuts at: 0 137 1724 2539 2648 2697
Size: 137 1587 815 109 49
Fragments arranged by size:
1587 815 137 109 49
BsaAI yAC'GTr
Cuts at: 0 2417 2697
Size: 2417 280
BsaHI Gr'CG_yC
Cuts at: 0 1394 1585 2697
Size: 1394 191 1112
Fragments arranged by size:
1394 1112 191
BsaWI w'CCGG_w
Cuts at: 0 866 2697
Size: 866 1831
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 719 749 932 962 1927 1957 2089 2119
Size: 719 30 183 30 965 30 132 30
Cuts at: 2119 2488 2518 2697
Size: 369 30 179
Fragments arranged by size:
965 719 369 183 179 132 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 54 953 1118 1127 1131 1948 2110 2536
Size: 54 899 165 9 4 817 162 426
Cuts at: 2536 2697
Size: 161
Fragments arranged by size:
899 817 426 165 162 161 54 9
4
BseYI C'CCAG_C
Cuts at: 0 348 641 1040 1234 1656 1769 1938 2281
Size: 348 293 399 194 422 113 169 343
Cuts at: 2281 2286 2369 2569 2634 2681 2697
Size: 5 83 200 65 47 16
Fragments arranged by size:
422 399 348 343 293 200 194 169
113 83 65 47 16 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 778 1024 1106 1459 1561 1576 1670 1999
Size: 778 246 82 353 102 15 94 329
Cuts at: 1999 2697
Size: 698
Fragments arranged by size:
778 698 353 329 246 102 94 82
15
BsiHKAI G_wGCw'C
Cuts at: 0 312 1329 1722 1740 1758 2696 2697
Size: 312 1017 393 18 18 938 1
Fragments arranged by size:
1017 938 393 312 18 18 1
BsiWI C'GTAC_G
Cuts at: 0 2597 2697
Size: 2597 100
Bsp1286I G_dGCh'C
Cuts at: 0 312 377 1180 1235 1329 1380 1722 1740
Size: 312 65 803 55 94 51 342 18
Cuts at: 1740 1758 1769 1830 2102 2110 2641 2666 2678
Size: 18 11 61 272 8 531 25 12
Cuts at: 2678 2696 2697
Size: 18 1
Fragments arranged by size:
803 531 342 312 272 94 65 61
55 51 25 18 18 18 12 11
8 1
BspEI T'CCGG_A
Cuts at: 0 866 2697
Size: 866 1831
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1445 1583 2476 2697
Size: 1445 138 893 221
Fragments arranged by size:
1445 893 221 138
BsrBI CCG'CTC
Cuts at: 0 479 900 1890 2697
Size: 479 421 990 807
Fragments arranged by size:
990 807 479 421
BsrDI GCAATG_nn'
Cuts at: 0 343 2697
Size: 343 2354
BssHII G'CGCG_C
Cuts at: 0 1342 1408 1410 1412 2697
Size: 1342 66 2 2 1285
Fragments arranged by size:
1342 1285 66 2 2
BssSI C'ACGA_G
Cuts at: 0 305 2697
Size: 305 2392
BstAPI GCAn_nnn'nTGC
Cuts at: 0 338 2697
Size: 338 2359
BstEII G'GTnAC_C
Cuts at: 0 1947 1959 2697
Size: 1947 12 738
Fragments arranged by size:
1947 738 12
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1069 1695 1929 2697
Size: 1069 626 234 768
Fragments arranged by size:
1069 768 626 234
BstYI r'GATC_y
Cuts at: 0 242 353 555 1848 2697
Size: 242 111 202 1293 849
Fragments arranged by size:
1293 849 242 202 111
Bsu36I CC'TnA_GG
Cuts at: 0 2687 2697
Size: 2687 10
BtgI C'CryG_G
Cuts at: 0 183 1181 1226 1259 1400 1491 2258 2670
Size: 183 998 45 33 141 91 767 412
Cuts at: 2670 2697
Size: 27
Fragments arranged by size:
998 767 412 183 141 91 45 33
27
BtsI GCAGTG_nn'
Cuts at: 0 1471 1738 2110 2697
Size: 1471 267 372 587
Fragments arranged by size:
1471 587 372 267
DraIII CAC_nnn'GTG
Cuts at: 0 295 307 2311 2440 2697
Size: 295 12 2004 129 257
Fragments arranged by size:
2004 295 257 129 12
DrdI GACnn_nn'nnGTC
Cuts at: 0 1393 2697
Size: 1393 1304
EaeI y'GGCC_r
Cuts at: 0 1220 2697
Size: 1220 1477
EarI CTCTTCn'nnn_
Cuts at: 0 992 2697
Size: 992 1705
EcoICRI GAG'CTC
Cuts at: 0 1327 1756 2697
Size: 1327 429 941
Fragments arranged by size:
1327 941 429
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 189 445 541 562 646 703 1130 1263
Size: 189 256 96 21 84 57 427 133
Cuts at: 1263 1729 1892 2083 2171 2353 2515 2697
Size: 466 163 191 88 182 162 182
Fragments arranged by size:
466 427 256 191 189 182 182 163
162 133 96 88 84 57 21
EcoNI CCTnn'n_nnAGG
Cuts at: 0 499 2062 2521 2697
Size: 499 1563 459 176
Fragments arranged by size:
1563 499 459 176
EcoO109I rG'GnC_Cy
Cuts at: 0 175 373 485 492 1177 1283 1305 1545
Size: 175 198 112 7 685 106 22 240
Cuts at: 1545 2155 2478 2499 2697
Size: 610 323 21 198
Fragments arranged by size:
685 610 323 240 198 198 175 112
106 22 21 7
FspI TGC'GCA
Cuts at: 0 2401 2455 2697
Size: 2401 54 242
Fragments arranged by size:
2401 242 54
HaeII r_GCGC'y
Cuts at: 0 1274 1373 1434 1702 1715 2697
Size: 1274 99 61 268 13 982
Fragments arranged by size:
1274 982 268 99 61 13
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 646 678 2125 2131 2157 2163 2488 2520
Size: 646 32 1447 6 26 6 325 32
Cuts at: 2520 2697
Size: 177
Fragments arranged by size:
1447 646 325 177 32 32 26 6
6
HincII GTy'rAC
Cuts at: 0 1560 2697
Size: 1560 1137
KpnI G_GTAC'C
Cuts at: 0 2040 2697
Size: 2040 657
MslI CAynn'nnrTG
Cuts at: 0 838 1067 1927 2221 2578 2697
Size: 838 229 860 294 357 119
Fragments arranged by size:
860 838 357 294 229 119
MspA1I CmG'CkG
Cuts at: 0 103 471 513 1008 1261 1402 1773 2026
Size: 103 368 42 495 253 141 371 253
Cuts at: 2026 2340 2369 2697
Size: 314 29 328
Fragments arranged by size:
495 371 368 328 314 253 253 141
103 42 29
NcoI C'CATG_G
Cuts at: 0 183 1181 2258 2697
Size: 183 998 1077 439
Fragments arranged by size:
1077 998 439 183
NdeI CA'TA_TG
Cuts at: 0 1781 2697
Size: 1781 916
NsiI A_TGCA'T
Cuts at: 0 1694 2697
Size: 1694 1003
NspI r_CATG'y
Cuts at: 0 421 2697
Size: 421 2276
PasI CC'CwG_GG
Cuts at: 0 489 2361 2697
Size: 489 1872 336
Fragments arranged by size:
1872 489 336
PflMI CCAn_nnn'nTGG
Cuts at: 0 324 2613 2697
Size: 324 2289 84
Fragments arranged by size:
2289 324 84
PfoI T'CCnGG_A
Cuts at: 0 1279 2697
Size: 1279 1418
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 766 798 2697
Size: 766 32 1899
Fragments arranged by size:
1899 766 32
PpuMI rG'GwC_Cy
Cuts at: 0 1283 1545 2478 2697
Size: 1283 262 933 219
Fragments arranged by size:
1283 933 262 219
PshAI GACnn'nnGTC
Cuts at: 0 1504 2697
Size: 1504 1193
PspOMI G'GGCC_C
Cuts at: 0 373 1176 2637 2697
Size: 373 803 1461 60
Fragments arranged by size:
1461 803 373 60
PstI C_TGCA'G
Cuts at: 0 1322 2327 2697
Size: 1322 1005 370
Fragments arranged by size:
1322 1005 370
PvuII CAG'CTG
Cuts at: 0 103 1773 2026 2340 2369 2697
Size: 103 1670 253 314 29 328
Fragments arranged by size:
1670 328 314 253 103 29
RsrII CG'GwC_CG
Cuts at: 0 1821 2697
Size: 1821 876
SacI G_AGCT'C
Cuts at: 0 1329 1758 2697
Size: 1329 429 939
Fragments arranged by size:
1329 939 429
SacII CC_GC'GG
Cuts at: 0 1262 1403 2697
Size: 1262 141 1294
Fragments arranged by size:
1294 1262 141
SexAI A'CCwGG_T
Cuts at: 0 116 281 2697
Size: 116 165 2416
Fragments arranged by size:
2416 165 116
SfcI C'TryA_G
Cuts at: 0 1152 1318 2323 2697
Size: 1152 166 1005 374
Fragments arranged by size:
1152 1005 374 166
SmaI CCC'GGG
Cuts at: 0 1196 2697
Size: 1196 1501
SmlI C'TyrA_G
Cuts at: 0 1328 2697
Size: 1328 1369
StuI AGG'CCT
Cuts at: 0 814 1024 2697
Size: 814 210 1673
Fragments arranged by size:
1673 814 210
StyI C'CwwG_G
Cuts at: 0 80 183 377 1181 2219 2258 2697
Size: 80 103 194 804 1038 39 439
Fragments arranged by size:
1038 804 439 194 103 80 39
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 397 1057 1966 2451 2697
Size: 397 660 909 485 246
Fragments arranged by size:
909 660 485 397 246
TatI w'GTAC_w
Cuts at: 0 1334 1809 2697
Size: 1334 475 888
Fragments arranged by size:
1334 888 475
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 932 964 2089 2121 2697
Size: 932 32 1125 32 576
Fragments arranged by size:
1125 932 576 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 273 2697
Size: 273 2424
XhoI C'TCGA_G
Cuts at: 0 1328 2697
Size: 1328 1369
XmaI C'CCGG_G
Cuts at: 0 1194 2697
Size: 1194 1503
Enzymes that do cut:
AarI AccI Acc65I AcuI AflIII AleI AlfI AlwNI
ApaI ApaLI AvaI BaeI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BsaAI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiHKAI BsiWI Bsp1286I BspEI BspMI
BsrBI BsrDI BssHII BssSI BstAPI BstEII BstXI BstYI
Bsu36I BtgI BtsI DraIII DrdI EaeI EarI EcoICRI
Eco57MI EcoNI EcoO109I FspI HaeII Hin4I HincII KpnI
MslI MspA1I NcoI NdeI NsiI NspI PasI PflMI
PfoI PpiI PpuMI PshAI PspOMI PstI PvuII RsrII
SacI SacII SexAI SfcI SmaI SmlI StuI StyI
TaqII TatI TstI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AclI AfeI AflII AgeI AhdI AloI ApoI
AscI AseI AsiSI AvrII BamHI BbeI BlpI BmtI
BpuEI BsaI BsaBI BsiEI BsmI BsmBI BspHI BsrFI
BsrGI BstBI BstZ17I BtgZI ClaI CspCI DraI EagI
EciI EcoRI EcoRV FalI FseI FspAI HindIII HpaI
KasI MfeI MluI MmeI MscI NaeI NarI NgoMIV
NheI NotI NruI PacI PciI PmeI PmlI PsiI
PspXI PsrI PvuI SalI SanDI SapI SbfI ScaI
SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI
SspI SwaI Tth111I XbaI XmnI ZraI