Restriction map for
pF1KB3419
(Linear) (Six-base) MAPSORT of: KIBB3419.seq Check: 4198 from: 1 to: 2400
KIBB3419.orf
With 182 enzymes: SgfI *
July 1, 2014 11:00 ..
AclI AA'CG_TT
Cuts at: 0 413 2400
Size: 413 1987
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 460 1055 1304 1985 2400
Size: 460 595 249 681 415
Fragments arranged by size:
681 595 460 415 249
AflIII A'CryG_T
Cuts at: 0 488 1581 2400
Size: 488 1093 819
Fragments arranged by size:
1093 819 488
AhdI GACnn_n'nnGTC
Cuts at: 0 402 2400
Size: 402 1998
AlwNI CAG_nnn'CTG
Cuts at: 0 1348 2400
Size: 1348 1052
ApaLI G'TGCA_C
Cuts at: 0 1533 2400
Size: 1533 867
ApoI r'AATT_y
Cuts at: 0 34 1236 2400
Size: 34 1202 1164
Fragments arranged by size:
1202 1164 34
BanI G'GyrC_C
Cuts at: 0 13 457 1857 2400
Size: 13 444 1400 543
Fragments arranged by size:
1400 543 444 13
BbsI GAAGACnn'nnnn_
Cuts at: 0 1028 1157 2400
Size: 1028 129 1243
Fragments arranged by size:
1243 1028 129
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1684 1718 2400
Size: 1684 34 682
Fragments arranged by size:
1684 682 34
BfrBI ATG'CAT
Cuts at: 0 354 2400
Size: 354 2046
BglI GCCn_nnn'nGGC
Cuts at: 0 2334 2400
Size: 2334 66
BglII A'GATC_T
Cuts at: 0 443 2400
Size: 443 1957
Bme1580I G_kGCm'C
Cuts at: 0 1537 1862 2400
Size: 1537 325 538
Fragments arranged by size:
1537 538 325
BmgBI CAC'GTC
Cuts at: 0 2047 2400
Size: 2047 353
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2274 2400
Size: 2274 126
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 506 799 1162 2400
Size: 506 293 363 1238
Fragments arranged by size:
1238 506 363 293
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 385 892 1683 2244 2400
Size: 385 507 791 561 156
Fragments arranged by size:
791 561 507 385 156
BsaI GGTCTCn'nnnn_
Cuts at: 0 389 2400
Size: 389 2011
BsaBI GATnn'nnATC
Cuts at: 0 311 2400
Size: 311 2089
BsaWI w'CCGG_w
Cuts at: 0 2318 2400
Size: 2318 82
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 772 802 835 865 2400
Size: 772 30 33 30 1535
Fragments arranged by size:
1535 772 33 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 130 313 2400
Size: 130 183 2087
Fragments arranged by size:
2087 183 130
BsiHKAI G_wGCw'C
Cuts at: 0 1537 2400
Size: 1537 863
BsmI GAATG_Cn'
Cuts at: 0 356 2103 2400
Size: 356 1747 297
Fragments arranged by size:
1747 356 297
BsmBI CGTCTCn'nnnn_
Cuts at: 0 11 1839 1913 2400
Size: 11 1828 74 487
Fragments arranged by size:
1828 487 74 11
Bsp1286I G_dGCh'C
Cuts at: 0 1537 1862 2400
Size: 1537 325 538
Fragments arranged by size:
1537 538 325
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1679 2400
Size: 1679 721
BssSI C'ACGA_G
Cuts at: 0 798 2400
Size: 798 1602
BstEII G'GTnAC_C
Cuts at: 0 1793 2400
Size: 1793 607
BstXI CCAn_nnnn'nTGG
Cuts at: 0 205 2400
Size: 205 2195
BstYI r'GATC_y
Cuts at: 0 443 2400
Size: 443 1957
Bsu36I CC'TnA_GG
Cuts at: 0 1616 2400
Size: 1616 784
BtgI C'CryG_G
Cuts at: 0 511 2382 2400
Size: 511 1871 18
Fragments arranged by size:
1871 511 18
DraI TTT'AAA
Cuts at: 0 618 944 1104 1463 2400
Size: 618 326 160 359 937
Fragments arranged by size:
937 618 359 326 160
DrdI GACnn_nn'nnGTC
Cuts at: 0 972 2284 2400
Size: 972 1312 116
Fragments arranged by size:
1312 972 116
EaeI y'GGCC_r
Cuts at: 0 748 1818 2400
Size: 748 1070 582
Fragments arranged by size:
1070 748 582
EarI CTCTTCn'nnn_
Cuts at: 0 1074 1947 2004 2267 2400
Size: 1074 873 57 263 133
Fragments arranged by size:
1074 873 263 133 57
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 856 2400
Size: 856 1544
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 460 506 799 1055 1162 1304 1985 2400
Size: 460 46 293 256 107 142 681 415
Fragments arranged by size:
681 460 415 293 256 142 107 46
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2117 2400
Size: 2117 283
EcoO109I rG'GnC_Cy
Cuts at: 0 609 2205 2400
Size: 609 1596 195
Fragments arranged by size:
1596 609 195
EcoRV GAT'ATC
Cuts at: 0 107 2400
Size: 107 2293
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 75 107 388 420 772 804 2400
Size: 75 32 281 32 352 32 1596
Fragments arranged by size:
1596 352 281 75 32 32 32
HindIII A'AGCT_T
Cuts at: 0 1095 2400
Size: 1095 1305
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 480 1001 1662 1718 1775 2400
Size: 480 521 661 56 57 625
Fragments arranged by size:
661 625 521 480 57 56
MscI TGG'CCA
Cuts at: 0 1820 2400
Size: 1820 580
MslI CAynn'nnrTG
Cuts at: 0 1814 2400
Size: 1814 586
MspA1I CmG'CkG
Cuts at: 0 987 2400
Size: 987 1413
NcoI C'CATG_G
Cuts at: 0 511 2400
Size: 511 1889
NdeI CA'TA_TG
Cuts at: 0 365 1545 2400
Size: 365 1180 855
Fragments arranged by size:
1180 855 365
NsiI A_TGCA'T
Cuts at: 0 356 2400
Size: 356 2044
NspI r_CATG'y
Cuts at: 0 492 1585 2400
Size: 492 1093 815
Fragments arranged by size:
1093 815 492
PasI CC'CwG_GG
Cuts at: 0 2201 2400
Size: 2201 199
PciI A'CATG_T
Cuts at: 0 488 1581 2400
Size: 488 1093 819
Fragments arranged by size:
1093 819 488
PflMI CCAn_nnn'nTGG
Cuts at: 0 1348 2400
Size: 1348 1052
PfoI T'CCnGG_A
Cuts at: 0 776 2400
Size: 776 1624
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1599 1631 2400
Size: 1599 32 769
Fragments arranged by size:
1599 769 32
PpuMI rG'GwC_Cy
Cuts at: 0 609 2205 2400
Size: 609 1596 195
Fragments arranged by size:
1596 609 195
ScaI AGT'ACT
Cuts at: 0 437 2400
Size: 437 1963
SfcI C'TryA_G
Cuts at: 0 2142 2400
Size: 2142 258
SmlI C'TyrA_G
Cuts at: 0 400 871 1698 2259 2400
Size: 400 471 827 561 141
Fragments arranged by size:
827 561 471 400 141
StyI C'CwwG_G
Cuts at: 0 494 511 1178 2400
Size: 494 17 667 1222
Fragments arranged by size:
1222 667 494 17
TatI w'GTAC_w
Cuts at: 0 435 2400
Size: 435 1965
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 789 821 835 867 1351 1383 2400
Size: 789 32 14 32 484 32 1017
Fragments arranged by size:
1017 789 484 32 32 32 14
Enzymes that do cut:
AclI AcuI AflIII AhdI AlwNI ApaLI ApoI BanI
BbsI BcgI BfrBI BglI BglII Bme1580I BmgBI BplI
BpmI BpuEI BsaI BsaBI BsaWI BsaXI BseRI BsiHKAI
BsmI BsmBI Bsp1286I BspMI BssSI BstEII BstXI BstYI
Bsu36I BtgI DraI DrdI EaeI EarI EciI Eco57MI
EcoNI EcoO109I EcoRV Hin4I HindIII MmeI MscI MslI
MspA1I NcoI NdeI NsiI NspI PasI PciI PflMI
PfoI PpiI PpuMI ScaI SfcI SmlI StyI TatI
TstI
Enzymes that do not cut:
AarI AatII AccI Acc65I AfeI AflII AgeI AleI
AlfI AloI ApaI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BanII BbeI BbvCI BciVI BclI BlpI
BmrI BmtI Bpu10I BsaAI BsaHI BseYI BsgI BsiEI
BsiWI BspEI BspHI BsrBI BsrDI BsrFI BsrGI BssHII
BstAPI BstBI BstZ17I BtgZI BtsI ClaI CspCI DraIII
EagI EcoICRI EcoRI FalI FseI FspI FspAI HaeII
HincII HpaI KasI KpnI MfeI MluI NaeI NarI
NgoMIV NheI NotI NruI PacI PmeI PmlI PshAI
PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI StuI SwaI TaqII Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI