(Linear) (Six-base) MAPSORT of: KIBB3421.seq  Check: 6796  from: 1  to: 729



KIBB3421.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:56  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     110     729 
   Size:        110     619
 
AflIII A'CryG_T

Cuts at:      0     521     729 
   Size:        521     208
 
AleI CACnn'nnGTG

Cuts at:      0     511     729 
   Size:        511     218
 
AvaI C'yCGr_G

Cuts at:      0      77     729 
   Size:         77     652
 
AvrII C'CTAG_G

Cuts at:      0     327     729 
   Size:        327     402
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     125     729 
   Size:        125     604
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     476     729 
   Size:        476     253
 
BsaAI yAC'GTr

Cuts at:      0     522     621     729 
   Size:        522      99     108

  Fragments arranged by size: 

                 522     108      99
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      98     128     473     503     729 
   Size:         98      30     345      30     226

  Fragments arranged by size: 

                 345     226      98      30      30
 
BseYI C'CCAG_C

Cuts at:      0     543     710     729 
   Size:        543     167      19

  Fragments arranged by size: 

                 543     167      19
 
BsrDI GCAATG_nn'

Cuts at:      0     293     729 
   Size:        293     436
 
BsrFI r'CCGG_y

Cuts at:      0     188     729 
   Size:        188     541
 
BtsI GCAGTG_nn'

Cuts at:      0      11     252     506     729 
   Size:         11     241     254     223

  Fragments arranged by size: 

                 254     241     223      11
 
EaeI y'GGCC_r

Cuts at:      0     272     729 
   Size:        272     457
 
EarI CTCTTCn'nnn_

Cuts at:      0      16     217     729 
   Size:         16     201     512

  Fragments arranged by size: 

                 512     201      16
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     207     729 
   Size:        207     522
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     110     125     729 
   Size:        110      15     604

  Fragments arranged by size: 

                 604     110      15
 
EcoO109I rG'GnC_Cy

Cuts at:      0     350     576     729 
   Size:        350     226     153

  Fragments arranged by size: 

                 350     226     153
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      85     117     729 
   Size:         85      32     612

  Fragments arranged by size: 

                 612      85      32
 
HincII GTy'rAC

Cuts at:      0     492     504     729 
   Size:        492      12     225

  Fragments arranged by size: 

                 492     225      12
 
HpaI GTT'AAC

Cuts at:      0     492     729 
   Size:        492     237
 
MscI TGG'CCA

Cuts at:      0     274     729 
   Size:        274     455
 
MslI CAynn'nnrTG

Cuts at:      0     511     553     729 
   Size:        511      42     176

  Fragments arranged by size: 

                 511     176      42
 
MspA1I CmG'CkG

Cuts at:      0     479     729 
   Size:        479     250
 
NaeI GCC'GGC

Cuts at:      0     190     729 
   Size:        190     539
 
NgoMIV G'CCGG_C

Cuts at:      0     188     729 
   Size:        188     541
 
PasI CC'CwG_GG

Cuts at:      0     572     729 
   Size:        572     157
 
PmlI CAC'GTG

Cuts at:      0     522     621     729 
   Size:        522      99     108

  Fragments arranged by size: 

                 522     108      99
 
PpuMI rG'GwC_Cy

Cuts at:      0     576     729 
   Size:        576     153
 
SanDI GG'GwC_CC

Cuts at:      0     576     729 
   Size:        576     153
 
SfcI C'TryA_G

Cuts at:      0     212     729 
   Size:        212     517
 
StyI C'CwwG_G

Cuts at:      0     327     729 
   Size:        327     402
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      98     130     729 
   Size:         98      32     599

  Fragments arranged by size: 

                 599      98      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     453     729 
   Size:        453     276
 

 Enzymes that do cut:

     AcuI   AflIII     AleI     AvaI    AvrII     BpmI     BsaI    BsaAI
    BsaXI    BseYI    BsrDI    BsrFI     BtsI     EaeI     EarI     EciI
  Eco57MI EcoO109I    Hin4I   HincII     HpaI     MscI     MslI   MspA1I
     NaeI   NgoMIV     PasI     PmlI    PpuMI    SanDI     SfcI     StyI
     TstI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI     BaeI    BamHI     BanI    BanII     BbeI     BbsI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI    BsaBI
    BsaHI    BsaWI    BseRI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI
    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I
     BtgI    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI     EagI
  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII  HindIII     KasI     KpnI     MfeI     MluI     MmeI     NarI
     NcoI     NdeI     NheI     NotI     NruI     NsiI     NspI     PacI
     PciI    PflMI     PfoI     PmeI     PpiI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII
     SalI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI    TaqII     TatI  Tth111I     XbaI     XhoI     XmaI
     XmnI     ZraI