Restriction map for
pF1KB3423
(Linear) (Six-base) MAPSORT of: KIBB3423.seq Check: 8812 from: 1 to: 1002
KIBB3423.orf
With 182 enzymes: SgfI *
July 18, 2007 13:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 588 660 1002
Size: 588 72 342
Fragments arranged by size:
588 342 72
AleI CACnn'nnGTG
Cuts at: 0 568 1002
Size: 568 434
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 746 1002
Size: 746 256
ApoI r'AATT_y
Cuts at: 0 93 319 1002
Size: 93 226 683
Fragments arranged by size:
683 226 93
AvaI C'yCGr_G
Cuts at: 0 71 1002
Size: 71 931
BbvCI CC'TCA_GC
Cuts at: 0 548 1002
Size: 548 454
BclI T'GATC_A
Cuts at: 0 179 324 1002
Size: 179 145 678
Fragments arranged by size:
678 179 145
BglI GCCn_nnn'nGGC
Cuts at: 0 640 1002
Size: 640 362
BlpI GC'TnA_GC
Cuts at: 0 446 617 756 1002
Size: 446 171 139 246
Fragments arranged by size:
446 246 171 139
Bpu10I CC'TnA_GC
Cuts at: 0 548 1002
Size: 548 454
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 764 1002
Size: 764 238
BsaBI GATnn'nnATC
Cuts at: 0 226 535 1002
Size: 226 309 467
Fragments arranged by size:
467 309 226
BsaWI w'CCGG_w
Cuts at: 0 89 1002
Size: 89 913
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 952 982 1002
Size: 952 30 20
Fragments arranged by size:
952 30 20
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 403 1002
Size: 403 599
BseYI C'CCAG_C
Cuts at: 0 44 761 935 1002
Size: 44 717 174 67
Fragments arranged by size:
717 174 67 44
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 139 206 622 742 808 1002
Size: 139 67 416 120 66 194
Fragments arranged by size:
416 194 139 120 67 66
BsmI GAATG_Cn'
Cuts at: 0 735 1002
Size: 735 267
BspEI T'CCGG_A
Cuts at: 0 89 1002
Size: 89 913
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 570 588 660 1002
Size: 570 18 72 342
Fragments arranged by size:
570 342 72 18
BsrFI r'CCGG_y
Cuts at: 0 733 994 1002
Size: 733 261 8
Fragments arranged by size:
733 261 8
BstAPI GCAn_nnn'nTGC
Cuts at: 0 675 1002
Size: 675 327
BstEII G'GTnAC_C
Cuts at: 0 990 1002
Size: 990 12
BstYI r'GATC_y
Cuts at: 0 704 713 797 896 1002
Size: 704 9 84 99 106
Fragments arranged by size:
704 106 99 84 9
BtgI C'CryG_G
Cuts at: 0 305 1002
Size: 305 697
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 522 1002
Size: 522 480
BtsI GCAGTG_nn'
Cuts at: 0 42 1002
Size: 42 960
ClaI AT'CG_AT
Cuts at: 0 344 1002
Size: 344 658
EaeI y'GGCC_r
Cuts at: 0 735 1002
Size: 735 267
EarI CTCTTCn'nnn_
Cuts at: 0 248 1002
Size: 248 754
EcoNI CCTnn'n_nnAGG
Cuts at: 0 770 1002
Size: 770 232
EcoO109I rG'GnC_Cy
Cuts at: 0 927 981 1002
Size: 927 54 21
Fragments arranged by size:
927 54 21
EcoRV GAT'ATC
Cuts at: 0 300 1002
Size: 300 702
HaeII r_GCGC'y
Cuts at: 0 196 279 1002
Size: 196 83 723
Fragments arranged by size:
723 196 83
HincII GTy'rAC
Cuts at: 0 239 1002
Size: 239 763
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 564 995 1002
Size: 564 431 7
Fragments arranged by size:
564 431 7
MslI CAynn'nnrTG
Cuts at: 0 568 670 1002
Size: 568 102 332
Fragments arranged by size:
568 332 102
MspA1I CmG'CkG
Cuts at: 0 460 726 741 1002
Size: 460 266 15 261
Fragments arranged by size:
460 266 261 15
NaeI GCC'GGC
Cuts at: 0 735 1002
Size: 735 267
NcoI C'CATG_G
Cuts at: 0 305 1002
Size: 305 697
NgoMIV G'CCGG_C
Cuts at: 0 733 1002
Size: 733 269
PpuMI rG'GwC_Cy
Cuts at: 0 927 1002
Size: 927 75
PshAI GACnn'nnGTC
Cuts at: 0 841 1002
Size: 841 161
PstI C_TGCA'G
Cuts at: 0 746 1002
Size: 746 256
PvuII CAG'CTG
Cuts at: 0 726 741 1002
Size: 726 15 261
Fragments arranged by size:
726 261 15
ScaI AGT'ACT
Cuts at: 0 430 1002
Size: 430 572
SfcI C'TryA_G
Cuts at: 0 55 286 742 1002
Size: 55 231 456 260
Fragments arranged by size:
456 260 231 55
SmlI C'TyrA_G
Cuts at: 0 779 1002
Size: 779 223
StyI C'CwwG_G
Cuts at: 0 209 305 1002
Size: 209 96 697
Fragments arranged by size:
697 209 96
TatI w'GTAC_w
Cuts at: 0 198 428 1002
Size: 198 230 574
Fragments arranged by size:
574 230 198
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 652 877 1002
Size: 652 225 125
Fragments arranged by size:
652 225 125
XmnI GAAnn'nnTTC
Cuts at: 0 109 1002
Size: 109 893
Enzymes that do cut:
AarI AleI AlfI ApoI AvaI BbvCI BclI BglI
BlpI Bpu10I BpuEI BsaBI BsaWI BsaXI BseRI BseYI
BsgI BsmI BspEI BspMI BsrFI BstAPI BstEII BstYI
BtgI BtgZI BtsI ClaI EaeI EarI EcoNI EcoO109I
EcoRV HaeII HincII MmeI MslI MspA1I NaeI NcoI
NgoMIV PpuMI PshAI PstI PvuII ScaI SfcI SmlI
StyI TatI XcmI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AloI AlwNI ApaI ApaLI AscI AseI
AsiSI AvrII BaeI BamHI BanI BanII BbeI BbsI
BcgI BciVI BfrBI BglII Bme1580I BmgBI BmrI BmtI
BplI BpmI BsaI BsaAI BsaHI BsiEI BsiHKAI BsiWI
BsmBI Bsp1286I BspHI BsrBI BsrDI BsrGI BssHII BssSI
BstBI BstXI BstZ17I Bsu36I CspCI DraI DraIII DrdI
EagI EciI EcoICRI Eco57MI EcoRI FalI FseI FspI
FspAI Hin4I HindIII HpaI KasI KpnI MfeI MluI
MscI NarI NdeI NheI NotI NruI NsiI NspI
PacI PasI PciI PflMI PfoI PmeI PmlI PpiI
PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII
SalI SanDI SapI SbfI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI
SwaI TaqII TstI Tth111I XbaI XhoI XmaI ZraI