Restriction map for
pF1KB3424
(Linear) (Six-base) MAPSORT of: KIBB3424.seq Check: 8458 from: 1 to: 1320
KIBB3424.orf
With 182 enzymes: SgfI *
March 3, 2008 10:56 ..
AccI GT'mk_AC
Cuts at: 0 370 416 1320
Size: 370 46 904
Fragments arranged by size:
904 370 46
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 208 842 1320
Size: 208 634 478
Fragments arranged by size:
634 478 208
AflII C'TTAA_G
Cuts at: 0 1218 1320
Size: 1218 102
AlwNI CAG_nnn'CTG
Cuts at: 0 133 1204 1320
Size: 133 1071 116
Fragments arranged by size:
1071 133 116
ApaI G_GGCC'C
Cuts at: 0 791 1320
Size: 791 529
ApoI r'AATT_y
Cuts at: 0 229 578 911 1320
Size: 229 349 333 409
Fragments arranged by size:
409 349 333 229
AvrII C'CTAG_G
Cuts at: 0 777 1320
Size: 777 543
BamHI G'GATC_C
Cuts at: 0 501 1011 1320
Size: 501 510 309
Fragments arranged by size:
510 501 309
BanI G'GyrC_C
Cuts at: 0 1297 1320
Size: 1297 23
BanII G_rGCy'C
Cuts at: 0 385 791 1320
Size: 385 406 529
Fragments arranged by size:
529 406 385
BbsI GAAGACnn'nnnn_
Cuts at: 0 1256 1320
Size: 1256 64
BbvCI CC'TCA_GC
Cuts at: 0 729 1320
Size: 729 591
BclI T'GATC_A
Cuts at: 0 1148 1320
Size: 1148 172
Bme1580I G_kGCm'C
Cuts at: 0 791 1320
Size: 791 529
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 53 1320
Size: 53 1267
Bpu10I CC'TnA_GC
Cuts at: 0 729 1320
Size: 729 591
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 762 1320
Size: 762 558
BsaI GGTCTCn'nnnn_
Cuts at: 0 538 1320
Size: 538 782
BsaBI GATnn'nnATC
Cuts at: 0 353 1320
Size: 353 967
BsaHI Gr'CG_yC
Cuts at: 0 863 1320
Size: 863 457
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 292 1320
Size: 292 1028
BsiWI C'GTAC_G
Cuts at: 0 798 1320
Size: 798 522
BsmI GAATG_Cn'
Cuts at: 0 401 1320
Size: 401 919
Bsp1286I G_dGCh'C
Cuts at: 0 385 791 1320
Size: 385 406 529
Fragments arranged by size:
529 406 385
BsrFI r'CCGG_y
Cuts at: 0 173 1320
Size: 173 1147
BsrGI T'GTAC_A
Cuts at: 0 1195 1320
Size: 1195 125
BstXI CCAn_nnnn'nTGG
Cuts at: 0 949 1320
Size: 949 371
BstYI r'GATC_y
Cuts at: 0 423 501 1011 1320
Size: 423 78 510 309
Fragments arranged by size:
510 423 309 78
Bsu36I CC'TnA_GG
Cuts at: 0 656 1304 1320
Size: 656 648 16
Fragments arranged by size:
656 648 16
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 77 112 879 914 1320
Size: 77 35 767 35 406
Fragments arranged by size:
767 406 77 35 35
DraIII CAC_nnn'GTG
Cuts at: 0 170 465 1320
Size: 170 295 855
Fragments arranged by size:
855 295 170
DrdI GACnn_nn'nnGTC
Cuts at: 0 318 1320
Size: 318 1002
EaeI y'GGCC_r
Cuts at: 0 986 1320
Size: 986 334
EarI CTCTTCn'nnn_
Cuts at: 0 332 629 1320
Size: 332 297 691
Fragments arranged by size:
691 332 297
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 53 208 842 1320
Size: 53 155 634 478
Fragments arranged by size:
634 478 155 53
EcoNI CCTnn'n_nnAGG
Cuts at: 0 686 1320
Size: 686 634
EcoO109I rG'GnC_Cy
Cuts at: 0 311 534 1320
Size: 311 223 786
Fragments arranged by size:
786 311 223
EcoRI G'AATT_C
Cuts at: 0 229 1320
Size: 229 1091
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 229 1107 1320
Size: 229 878 213
Fragments arranged by size:
878 229 213
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 6 38 271 303 1153 1185 1320
Size: 6 32 233 32 850 32 135
Fragments arranged by size:
850 233 135 32 32 32 6
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 548 908 926 1320
Size: 548 360 18 394
Fragments arranged by size:
548 394 360 18
MscI TGG'CCA
Cuts at: 0 988 1320
Size: 988 332
MslI CAynn'nnrTG
Cuts at: 0 517 1320
Size: 517 803
NspI r_CATG'y
Cuts at: 0 472 832 1320
Size: 472 360 488
Fragments arranged by size:
488 472 360
PfoI T'CCnGG_A
Cuts at: 0 30 1320
Size: 30 1290
PpuMI rG'GwC_Cy
Cuts at: 0 311 1320
Size: 311 1009
PsiI TTA'TAA
Cuts at: 0 1283 1320
Size: 1283 37
PspOMI G'GGCC_C
Cuts at: 0 787 1320
Size: 787 533
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 68 100 1320
Size: 68 32 1220
Fragments arranged by size:
1220 68 32
SanDI GG'GwC_CC
Cuts at: 0 311 1320
Size: 311 1009
SmlI C'TyrA_G
Cuts at: 0 741 1218 1320
Size: 741 477 102
Fragments arranged by size:
741 477 102
SphI G_CATG'C
Cuts at: 0 472 1320
Size: 472 848
SspI AAT'ATT
Cuts at: 0 628 1320
Size: 628 692
StyI C'CwwG_G
Cuts at: 0 332 777 1320
Size: 332 445 543
Fragments arranged by size:
543 445 332
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 435 631 1320
Size: 435 196 689
Fragments arranged by size:
689 435 196
TatI w'GTAC_w
Cuts at: 0 1195 1320
Size: 1195 125
XmnI GAAnn'nnTTC
Cuts at: 0 764 1274 1320
Size: 764 510 46
Fragments arranged by size:
764 510 46
Enzymes that do cut:
AccI AcuI AflII AlwNI ApaI ApoI AvrII BamHI
BanI BanII BbsI BbvCI BclI Bme1580I BpmI Bpu10I
BpuEI BsaI BsaBI BsaHI BseRI BsiWI BsmI Bsp1286I
BsrFI BsrGI BstXI BstYI Bsu36I CspCI DraIII DrdI
EaeI EarI Eco57MI EcoNI EcoO109I EcoRI FalI Hin4I
MmeI MscI MslI NspI PfoI PpuMI PsiI PspOMI
PsrI SanDI SmlI SphI SspI StyI TaqII TatI
XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflIII AgeI AhdI
AleI AlfI AloI ApaLI AscI AseI AsiSI AvaI
BaeI BbeI BcgI BciVI BfrBI BglI BglII BlpI
BmgBI BmrI BmtI BplI BsaAI BsaWI BsaXI BseYI
BsgI BsiEI BsiHKAI BsmBI BspEI BspHI BspMI BsrBI
BsrDI BssHII BssSI BstAPI BstBI BstEII BstZ17I BtgI
BtgZI BtsI ClaI DraI EagI EciI EcoICRI EcoRV
FseI FspI FspAI HaeII HincII HindIII HpaI KasI
KpnI MfeI MluI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI PacI PasI PciI
PflMI PmeI PmlI PpiI PshAI PspXI PstI PvuI
PvuII RsrII SacI SacII SalI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SrfI StuI SwaI TstI Tth111I XbaI XcmI
XhoI XmaI ZraI