(Linear) (Six-base) MAPSORT of: KIBB3432.seq  Check: 446  from: 1  to: 342



KIBB3432.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:01  ..

AlwNI CAG_nnn'CTG

Cuts at:      0     214     342 
   Size:        214     128
 
BanII G_rGCy'C

Cuts at:      0      20     342 
   Size:         20     322
 
BbvCI CC'TCA_GC

Cuts at:      0     266     342 
   Size:        266      76
 
BglI GCCn_nnn'nGGC

Cuts at:      0     126     342 
   Size:        126     216
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     191     245     342 
   Size:        191      54      97

  Fragments arranged by size: 

                 191      97      54
 
Bpu10I CC'TnA_GC

Cuts at:      0     266     342 
   Size:        266      76
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     283     319     342 
   Size:        283      36      23

  Fragments arranged by size: 

                 283      36      23
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      31     206     342 
   Size:         31     175     136

  Fragments arranged by size: 

                 175     136      31
 
BsiHKAI G_wGCw'C

Cuts at:      0      20     342 
   Size:         20     322
 
Bsp1286I G_dGCh'C

Cuts at:      0      20     342 
   Size:         20     322
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     210     342 
   Size:        210     132
 
BsrDI GCAATG_nn'

Cuts at:      0     189     342 
   Size:        189     153
 
BsrFI r'CCGG_y

Cuts at:      0     103     193     342 
   Size:        103      90     149

  Fragments arranged by size: 

                 149     103      90
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     217     342 
   Size:        217     125
 
BtsI GCAGTG_nn'

Cuts at:      0     248     342 
   Size:        248      94
 
EaeI y'GGCC_r

Cuts at:      0     152     342 
   Size:        152     190
 
EcoICRI GAG'CTC

Cuts at:      0      18     342 
   Size:         18     324
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     191     245     342 
   Size:        191      54      97

  Fragments arranged by size: 

                 191      97      54
 
EcoO109I rG'GnC_Cy

Cuts at:      0     147     235     342 
   Size:        147      88     107

  Fragments arranged by size: 

                 147     107      88
 
HincII GTy'rAC

Cuts at:      0     162     342 
   Size:        162     180
 
MscI TGG'CCA

Cuts at:      0     154     342 
   Size:        154     188
 
MslI CAynn'nnrTG

Cuts at:      0      53     342 
   Size:         53     289
 
MspA1I CmG'CkG

Cuts at:      0     214     265     342 
   Size:        214      51      77

  Fragments arranged by size: 

                 214      77      51
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     214     342 
   Size:        214     128
 
PpuMI rG'GwC_Cy

Cuts at:      0     235     342 
   Size:        235     107
 
PvuII CAG'CTG

Cuts at:      0     214     265     342 
   Size:        214      51      77

  Fragments arranged by size: 

                 214      77      51
 
SacI G_AGCT'C

Cuts at:      0      20     342 
   Size:         20     322
 

 Enzymes that do cut:

    AlwNI    BanII    BbvCI     BglI     BpmI   Bpu10I    BseRI     BsgI
  BsiHKAI Bsp1286I    BspMI    BsrDI    BsrFI   BstAPI     BtsI     EaeI
  EcoICRI  Eco57MI EcoO109I   HincII     MscI     MslI   MspA1I    PflMI
    PpuMI    PvuII     SacI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BanI     BbeI     BbsI     BcgI    BciVI     BclI    BfrBI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseYI    BsiEI    BsiWI
     BsmI    BsmBI    BspEI    BspHI    BsrBI    BsrGI   BssHII    BssSI
    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI
     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EarI     EciI
    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII
    Hin4I  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI     PfoI     PmeI     PmlI
     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    RsrII    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI