Restriction map for
pF1KB3433
(Linear) (Six-base) MAPSORT of: KIBB3433.seq Check: 9090 from: 1 to: 1281
KIBB3433.orf
With 182 enzymes: SgfI *
March 3, 2008 10:56 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 645 1281
Size: 645 636
AflII C'TTAA_G
Cuts at: 0 511 1281
Size: 511 770
AflIII A'CryG_T
Cuts at: 0 281 1281
Size: 281 1000
AlwNI CAG_nnn'CTG
Cuts at: 0 1066 1225 1281
Size: 1066 159 56
Fragments arranged by size:
1066 159 56
ApaI G_GGCC'C
Cuts at: 0 619 1281
Size: 619 662
ApoI r'AATT_y
Cuts at: 0 224 1281
Size: 224 1057
AseI AT'TA_AT
Cuts at: 0 854 1281
Size: 854 427
BanI G'GyrC_C
Cuts at: 0 323 1281
Size: 323 958
BanII G_rGCy'C
Cuts at: 0 619 903 1281
Size: 619 284 378
Fragments arranged by size:
619 378 284
BbeI G_GCGC'C
Cuts at: 0 327 1281
Size: 327 954
BbsI GAAGACnn'nnnn_
Cuts at: 0 493 1281
Size: 493 788
BbvCI CC'TCA_GC
Cuts at: 0 390 1281
Size: 390 891
BciVI GTATCCnnnnn_n'
Cuts at: 0 613 1281
Size: 613 668
BglII A'GATC_T
Cuts at: 0 861 1281
Size: 861 420
Bme1580I G_kGCm'C
Cuts at: 0 619 1281
Size: 619 662
Bpu10I CC'TnA_GC
Cuts at: 0 390 1281
Size: 390 891
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 802 984 1281
Size: 802 182 297
Fragments arranged by size:
802 297 182
BsaHI Gr'CG_yC
Cuts at: 0 324 1281
Size: 324 957
BsaWI w'CCGG_w
Cuts at: 0 770 1281
Size: 770 511
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1276 1281
Size: 1276 5
BsiHKAI G_wGCw'C
Cuts at: 0 12 912 1281
Size: 12 900 369
Fragments arranged by size:
900 369 12
Bsp1286I G_dGCh'C
Cuts at: 0 12 619 903 912 1281
Size: 12 607 284 9 369
Fragments arranged by size:
607 369 284 12 9
BspEI T'CCGG_A
Cuts at: 0 770 1281
Size: 770 511
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 163 1281
Size: 163 1118
BsrBI CCG'CTC
Cuts at: 0 257 1281
Size: 257 1024
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1066 1281
Size: 1066 215
BstYI r'GATC_y
Cuts at: 0 861 890 923 1281
Size: 861 29 33 358
Fragments arranged by size:
861 358 33 29
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 913 1281
Size: 913 368
EaeI y'GGCC_r
Cuts at: 0 261 1281
Size: 261 1020
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 645 1281
Size: 645 636
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1130 1281
Size: 1130 151
EcoO109I rG'GnC_Cy
Cuts at: 0 615 1281
Size: 615 666
EcoRI G'AATT_C
Cuts at: 0 224 1281
Size: 224 1057
EcoRV GAT'ATC
Cuts at: 0 23 537 1281
Size: 23 514 744
Fragments arranged by size:
744 514 23
FspI TGC'GCA
Cuts at: 0 133 1281
Size: 133 1148
HaeII r_GCGC'y
Cuts at: 0 327 1281
Size: 327 954
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 353 385 579 611 1281
Size: 353 32 194 32 670
Fragments arranged by size:
670 353 194 32 32
HindIII A'AGCT_T
Cuts at: 0 715 1281
Size: 715 566
KasI G'GCGC_C
Cuts at: 0 323 1281
Size: 323 958
MscI TGG'CCA
Cuts at: 0 263 1281
Size: 263 1018
MspA1I CmG'CkG
Cuts at: 0 1063 1075 1281
Size: 1063 12 206
Fragments arranged by size:
1063 206 12
NarI GG'CG_CC
Cuts at: 0 324 1281
Size: 324 957
NdeI CA'TA_TG
Cuts at: 0 990 1281
Size: 990 291
NspI r_CATG'y
Cuts at: 0 285 736 1281
Size: 285 451 545
Fragments arranged by size:
545 451 285
PciI A'CATG_T
Cuts at: 0 281 1281
Size: 281 1000
PflMI CCAn_nnn'nTGG
Cuts at: 0 320 1281
Size: 320 961
PspOMI G'GGCC_C
Cuts at: 0 615 1281
Size: 615 666
PstI C_TGCA'G
Cuts at: 0 1071 1242 1281
Size: 1071 171 39
Fragments arranged by size:
1071 171 39
PvuII CAG'CTG
Cuts at: 0 1063 1281
Size: 1063 218
SfcI C'TryA_G
Cuts at: 0 1067 1238 1256 1281
Size: 1067 171 18 25
Fragments arranged by size:
1067 171 25 18
SfoI GGC'GCC
Cuts at: 0 325 1281
Size: 325 956
SmlI C'TyrA_G
Cuts at: 0 511 817 963 1281
Size: 511 306 146 318
Fragments arranged by size:
511 318 306 146
SpeI A'CTAG_T
Cuts at: 0 184 1281
Size: 184 1097
StuI AGG'CCT
Cuts at: 0 658 1281
Size: 658 623
StyI C'CwwG_G
Cuts at: 0 491 1281
Size: 491 790
TatI w'GTAC_w
Cuts at: 0 29 1281
Size: 29 1252
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 747 779 1281
Size: 747 32 502
Fragments arranged by size:
747 502 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 271 317 1281
Size: 271 46 964
Fragments arranged by size:
964 271 46
Enzymes that do cut:
AcuI AflII AflIII AlwNI ApaI ApoI AseI BanI
BanII BbeI BbsI BbvCI BciVI BglII Bme1580I Bpu10I
BpuEI BsaHI BsaWI BseRI BsiHKAI Bsp1286I BspEI BspMI
BsrBI BstAPI BstYI BtgZI EaeI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FspI HaeII Hin4I HindIII KasI MscI
MspA1I NarI NdeI NspI PciI PflMI PspOMI PstI
PvuII SfcI SfoI SmlI SpeI StuI StyI TatI
TstI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AgeI AhdI
AleI AlfI AloI ApaLI AscI AsiSI AvaI AvrII
BaeI BamHI BcgI BclI BfrBI BglI BlpI BmgBI
BmrI BmtI BplI BpmI BsaI BsaAI BsaBI BsaXI
BseYI BsgI BsiEI BsiWI BsmI BsmBI BspHI BsrDI
BsrFI BsrGI BssHII BssSI BstBI BstEII BstXI BstZ17I
Bsu36I BtgI BtsI ClaI CspCI DraI DraIII DrdI
EagI EarI EciI EcoICRI FalI FseI FspAI HincII
HpaI KpnI MfeI MluI MmeI MslI NaeI NcoI
NgoMIV NheI NotI NruI NsiI PacI PasI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI
PvuI RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI SphI
SrfI SspI SwaI TaqII Tth111I XbaI XhoI XmaI
XmnI ZraI