Restriction map for
pF1KB3434
(Linear) (Six-base) MAPSORT of: KIBB3434.seq Check: 5379 from: 1 to: 969
KIBB3434.orf
With 182 enzymes: SgfI *
April 24, 2009 13:01 ..
Acc65I G'GTAC_C
Cuts at: 0 674 969
Size: 674 295
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 163 553 969
Size: 163 390 416
Fragments arranged by size:
416 390 163
AflIII A'CryG_T
Cuts at: 0 389 969
Size: 389 580
AleI CACnn'nnGTG
Cuts at: 0 403 969
Size: 403 566
AlwNI CAG_nnn'CTG
Cuts at: 0 426 969
Size: 426 543
ApaLI G'TGCA_C
Cuts at: 0 609 724 969
Size: 609 115 245
Fragments arranged by size:
609 245 115
AscI GG'CGCG_CC
Cuts at: 0 735 969
Size: 735 234
AvaI C'yCGr_G
Cuts at: 0 65 808 877 969
Size: 65 743 69 92
Fragments arranged by size:
743 92 69 65
AvrII C'CTAG_G
Cuts at: 0 162 969
Size: 162 807
BanI G'GyrC_C
Cuts at: 0 103 674 817 969
Size: 103 571 143 152
Fragments arranged by size:
571 152 143 103
BanII G_rGCy'C
Cuts at: 0 66 815 969
Size: 66 749 154
Fragments arranged by size:
749 154 66
BbeI G_GCGC'C
Cuts at: 0 821 969
Size: 821 148
BglI GCCn_nnn'nGGC
Cuts at: 0 347 714 969
Size: 347 367 255
Fragments arranged by size:
367 347 255
BlpI GC'TnA_GC
Cuts at: 0 847 969
Size: 847 122
Bme1580I G_kGCm'C
Cuts at: 0 108 613 728 969
Size: 108 505 115 241
Fragments arranged by size:
505 241 115 108
BmgBI CAC'GTC
Cuts at: 0 378 969
Size: 378 591
BmrI ACTGGGnnnn_n'
Cuts at: 0 234 969
Size: 234 735
Bpu10I CC'TnA_GC
Cuts at: 0 587 969
Size: 587 382
BsaBI GATnn'nnATC
Cuts at: 0 191 969
Size: 191 778
BsaHI Gr'CG_yC
Cuts at: 0 818 939 969
Size: 818 121 30
Fragments arranged by size:
818 121 30
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 895 925 969
Size: 895 30 44
Fragments arranged by size:
895 44 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 127 130 250 271 274 969
Size: 127 3 120 21 3 695
Fragments arranged by size:
695 127 120 21 3 3
BseYI C'CCAG_C
Cuts at: 0 38 95 175 292 717 821 826 969
Size: 38 57 80 117 425 104 5 143
Fragments arranged by size:
425 143 117 104 80 57 38 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 349 358 370 969
Size: 349 9 12 599
Fragments arranged by size:
599 349 12 9
BsiEI CG_ry'CG
Cuts at: 0 516 732 969
Size: 516 216 237
Fragments arranged by size:
516 237 216
BsiHKAI G_wGCw'C
Cuts at: 0 512 613 728 815 969
Size: 512 101 115 87 154
Fragments arranged by size:
512 154 115 101 87
BsmBI CGTCTCn'nnnn_
Cuts at: 0 946 969
Size: 946 23
Bsp1286I G_dGCh'C
Cuts at: 0 66 108 512 613 728 815 969
Size: 66 42 404 101 115 87 154
Fragments arranged by size:
404 154 115 101 87 66 42
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 686 969
Size: 686 283
BsrBI CCG'CTC
Cuts at: 0 120 846 969
Size: 120 726 123
Fragments arranged by size:
726 123 120
BsrFI r'CCGG_y
Cuts at: 0 346 639 969
Size: 346 293 330
Fragments arranged by size:
346 330 293
BssHII G'CGCG_C
Cuts at: 0 681 735 969
Size: 681 54 234
Fragments arranged by size:
681 234 54
BstAPI GCAn_nnn'nTGC
Cuts at: 0 372 477 969
Size: 372 105 492
Fragments arranged by size:
492 372 105
BstBI TT'CG_AA
Cuts at: 0 581 758 969
Size: 581 177 211
Fragments arranged by size:
581 211 177
Bsu36I CC'TnA_GG
Cuts at: 0 311 969
Size: 311 658
BtgI C'CryG_G
Cuts at: 0 57 969
Size: 57 912
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 476 778 969
Size: 476 302 191
Fragments arranged by size:
476 302 191
EaeI y'GGCC_r
Cuts at: 0 54 344 513 765 969
Size: 54 290 169 252 204
Fragments arranged by size:
290 252 204 169 54
EagI C'GGCC_G
Cuts at: 0 513 969
Size: 513 456
EarI CTCTTCn'nnn_
Cuts at: 0 248 760 969
Size: 248 512 209
Fragments arranged by size:
512 248 209
EcoICRI GAG'CTC
Cuts at: 0 813 969
Size: 813 156
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 163 553 969
Size: 163 390 416
Fragments arranged by size:
416 390 163
EcoNI CCTnn'n_nnAGG
Cuts at: 0 481 969
Size: 481 488
EcoO109I rG'GnC_Cy
Cuts at: 0 132 744 969
Size: 132 612 225
Fragments arranged by size:
612 225 132
FseI GG_CCGG'CC
Cuts at: 0 350 969
Size: 350 619
HaeII r_GCGC'y
Cuts at: 0 667 691 821 833 969
Size: 667 24 130 12 136
Fragments arranged by size:
667 136 130 24 12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 895 927 969
Size: 895 32 42
Fragments arranged by size:
895 42 32
KasI G'GCGC_C
Cuts at: 0 817 969
Size: 817 152
KpnI G_GTAC'C
Cuts at: 0 678 969
Size: 678 291
MluI A'CGCG_T
Cuts at: 0 389 969
Size: 389 580
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 374 969
Size: 374 595
MscI TGG'CCA
Cuts at: 0 56 969
Size: 56 913
MslI CAynn'nnrTG
Cuts at: 0 403 969
Size: 403 566
MspA1I CmG'CkG
Cuts at: 0 372 435 936 969
Size: 372 63 501 33
Fragments arranged by size:
501 372 63 33
NaeI GCC'GGC
Cuts at: 0 348 641 969
Size: 348 293 328
Fragments arranged by size:
348 328 293
NarI GG'CG_CC
Cuts at: 0 818 969
Size: 818 151
NcoI C'CATG_G
Cuts at: 0 57 969
Size: 57 912
NgoMIV G'CCGG_C
Cuts at: 0 346 639 969
Size: 346 293 330
Fragments arranged by size:
346 330 293
PasI CC'CwG_GG
Cuts at: 0 420 969
Size: 420 549
PflMI CCAn_nnn'nTGG
Cuts at: 0 45 657 969
Size: 45 612 312
Fragments arranged by size:
612 312 45
PstI C_TGCA'G
Cuts at: 0 155 482 969
Size: 155 327 487
Fragments arranged by size:
487 327 155
PvuI CG_AT'CG
Cuts at: 0 732 969
Size: 732 237
PvuII CAG'CTG
Cuts at: 0 372 435 969
Size: 372 63 534
Fragments arranged by size:
534 372 63
SacI G_AGCT'C
Cuts at: 0 815 969
Size: 815 154
SbfI CC_TGCA'GG
Cuts at: 0 482 969
Size: 482 487
SfcI C'TryA_G
Cuts at: 0 151 478 969
Size: 151 327 491
Fragments arranged by size:
491 327 151
SfoI GGC'GCC
Cuts at: 0 819 969
Size: 819 150
SmlI C'TyrA_G
Cuts at: 0 877 969
Size: 877 92
StuI AGG'CCT
Cuts at: 0 362 898 969
Size: 362 536 71
Fragments arranged by size:
536 362 71
StyI C'CwwG_G
Cuts at: 0 57 162 969
Size: 57 105 807
Fragments arranged by size:
807 105 57
Tth111I GACn'n_nGTC
Cuts at: 0 319 969
Size: 319 650
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 865 969
Size: 865 104
XhoI C'TCGA_G
Cuts at: 0 877 969
Size: 877 92
Enzymes that do cut:
Acc65I AcuI AflIII AleI AlwNI ApaLI AscI AvaI
AvrII BanI BanII BbeI BglI BlpI Bme1580I BmgBI
BmrI Bpu10I BsaBI BsaHI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrFI BssHII
BstAPI BstBI Bsu36I BtgI BtgZI EaeI EagI EarI
EcoICRI Eco57MI EcoNI EcoO109I FseI HaeII Hin4I KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV PasI PflMI PstI PvuI PvuII SacI
SbfI SfcI SfoI SmlI StuI StyI Tth111I XcmI
XhoI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AgeI AhdI
AlfI AloI ApaI ApoI AseI AsiSI BaeI BamHI
BbsI BbvCI BcgI BciVI BclI BfrBI BglII BmtI
BplI BpmI BpuEI BsaI BsaAI BsaWI BsiWI BsmI
BspEI BspHI BsrDI BsrGI BssSI BstEII BstXI BstYI
BstZ17I BtsI ClaI CspCI DraI DraIII DrdI EciI
EcoRI EcoRV FalI FspI FspAI HincII HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI NspI PacI
PciI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PspXI PsrI RsrII SacII SalI SanDI SapI
ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI SwaI TaqII TatI TstI XbaI
XmaI XmnI ZraI