Restriction map for pF1KB3440
 (Linear) (Six-base) MAPSORT of: KIBB3440.seq  Check: 1947  from: 1  to: 774



KIBB3440.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:01  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     604     774 
   Size:        604     170
 
AccI GT'mk_AC

Cuts at:      0     590     774 
   Size:        590     184
 
AfeI AGC'GCT

Cuts at:      0     154     774 
   Size:        154     620
 
AflIII A'CryG_T

Cuts at:      0     134     774 
   Size:        134     640
 
AleI CACnn'nnGTG

Cuts at:      0      73     774 
   Size:         73     701
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      35     751     774 
   Size:         35     716      23

  Fragments arranged by size: 

                 716      35      23
 
AlwNI CAG_nnn'CTG

Cuts at:      0     552     774 
   Size:        552     222
 
ApaI G_GGCC'C

Cuts at:      0     344     404     774 
   Size:        344      60     370

  Fragments arranged by size: 

                 370     344      60
 
AvrII C'CTAG_G

Cuts at:      0     370     774 
   Size:        370     404
 
BanII G_rGCy'C

Cuts at:      0     344     404     710     774 
   Size:        344      60     306      64

  Fragments arranged by size: 

                 344     306      64      60
 
Bme1580I G_kGCm'C

Cuts at:      0     344     404     774 
   Size:        344      60     370

  Fragments arranged by size: 

                 370     344      60
 
BmgBI CAC'GTC

Cuts at:      0     210     774 
   Size:        210     564
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      80     181     304     774 
   Size:         80     101     123     470

  Fragments arranged by size: 

                 470     123     101      80
 
BsaAI yAC'GTr

Cuts at:      0     435     774 
   Size:        435     339
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     199     268     301     759     774 
   Size:        199      69      33     458      15

  Fragments arranged by size: 

                 458     199      69      33      15
 
BseYI C'CCAG_C

Cuts at:      0     237     333     499     575     688     702     774 
   Size:        237      96     166      76     113      14      72

  Fragments arranged by size: 

                 237     166     113      96      76      72      14
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     336     774 
   Size:        336     438
 
BsiHKAI G_wGCw'C

Cuts at:      0     611     774 
   Size:        611     163
 
BsmI GAATG_Cn'

Cuts at:      0     133     458     774 
   Size:        133     325     316

  Fragments arranged by size: 

                 325     316     133
 
Bsp1286I G_dGCh'C

Cuts at:      0     344     404     611     710     774 
   Size:        344      60     207      99      64

  Fragments arranged by size: 

                 344     207      99      64      60
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0       3     428     604     774 
   Size:          3     425     176     170

  Fragments arranged by size: 

                 425     176     170       3
 
BstYI r'GATC_y

Cuts at:      0     548     766     774 
   Size:        548     218       8

  Fragments arranged by size: 

                 548     218       8
 
BtgI C'CryG_G

Cuts at:      0     635     774 
   Size:        635     139
 
BtsI GCAGTG_nn'

Cuts at:      0     305     774 
   Size:        305     469
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      80     181     304     774 
   Size:         80     101     123     470

  Fragments arranged by size: 

                 470     123     101      80
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     112     774 
   Size:        112     662
 
EcoO109I rG'GnC_Cy

Cuts at:      0     400     401     774 
   Size:        400       1     373

  Fragments arranged by size: 

                 400     373       1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     420     774 
   Size:        420     354
 
HaeII r_GCGC'y

Cuts at:      0     156     360     774 
   Size:        156     204     414

  Fragments arranged by size: 

                 414     204     156
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      24      56     713     745     774 
   Size:         24      32     657      32      29

  Fragments arranged by size: 

                 657      32      32      29      24
 
MluI A'CGCG_T

Cuts at:      0     134     774 
   Size:        134     640
 
MslI CAynn'nnrTG

Cuts at:      0      73     733     774 
   Size:         73     660      41

  Fragments arranged by size: 

                 660      73      41
 
MspA1I CmG'CkG

Cuts at:      0     499     774 
   Size:        499     275
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     552     774 
   Size:        552     222
 
PfoI T'CCnGG_A

Cuts at:      0     158     281     774 
   Size:        158     123     493

  Fragments arranged by size: 

                 493     158     123
 
PmlI CAC'GTG

Cuts at:      0     435     774 
   Size:        435     339
 
PspOMI G'GGCC_C

Cuts at:      0     340     400     774 
   Size:        340      60     374

  Fragments arranged by size: 

                 374     340      60
 
PstI C_TGCA'G

Cuts at:      0      14     356     774 
   Size:         14     342     418

  Fragments arranged by size: 

                 418     342      14
 
PvuII CAG'CTG

Cuts at:      0     499     774 
   Size:        499     275
 
ScaI AGT'ACT

Cuts at:      0     259     774 
   Size:        259     515
 
SfcI C'TryA_G

Cuts at:      0      10     352     774 
   Size:         10     342     422

  Fragments arranged by size: 

                 422     342      10
 
StyI C'CwwG_G

Cuts at:      0     370     774 
   Size:        370     404
 
TatI w'GTAC_w

Cuts at:      0     257     774 
   Size:        257     517
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     140     172     298     330     774 
   Size:        140      32     126      32     444

  Fragments arranged by size: 

                 444     140     126      32      32
 

 Enzymes that do cut:

     AarI     AccI     AfeI   AflIII     AleI     AlfI    AlwNI     ApaI
    AvrII    BanII Bme1580I    BmgBI     BpmI    BsaAI    BseRI    BseYI
     BsgI  BsiHKAI     BsmI Bsp1286I    BspMI    BstYI     BtgI     BtsI
  Eco57MI    EcoNI EcoO109I     FalI    HaeII    Hin4I     MluI     MslI
   MspA1I    PflMI     PfoI     PmlI   PspOMI     PstI    PvuII     ScaI
     SfcI     StyI     TatI     TstI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AcuI    AflII     AgeI     AhdI     AloI
    ApaLI     ApoI     AscI     AseI    AsiSI     AvaI     BaeI    BamHI
     BanI     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI    BglII     BlpI     BmrI     BmtI     BplI   Bpu10I    BpuEI
     BsaI    BsaBI    BsaHI    BsaWI    BsaXI    BsiEI    BsiWI    BsmBI
    BspEI    BspHI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI     ClaI
    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EarI     EciI
  EcoICRI    EcoRI    EcoRV     FseI     FspI    FspAI   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MmeI     MscI     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI     PmeI     PpiI    PpuMI    PshAI     PsiI
    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI
    TaqII  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI
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