(Linear) (Six-base) MAPSORT of: KIBB3441.seq Check: 5051 from: 1 to: 1020 KIBB3441.orf With 182 enzymes: SgfI * March 3, 2008 10:56 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 754 904 1020 Size: 754 150 116 Fragments arranged by size: 754 150 116 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 602 1020 Size: 602 418 AflII C'TTAA_G Cuts at: 0 40 1020 Size: 40 980 AflIII A'CryG_T Cuts at: 0 493 1020 Size: 493 527 AhdI GACnn_n'nnGTC Cuts at: 0 595 1020 Size: 595 425 BanI G'GyrC_C Cuts at: 0 158 845 1020 Size: 158 687 175 Fragments arranged by size: 687 175 158 BanII G_rGCy'C Cuts at: 0 782 1020 Size: 782 238 BbeI G_GCGC'C Cuts at: 0 849 1020 Size: 849 171 BfrBI ATG'CAT Cuts at: 0 718 1020 Size: 718 302 BglI GCCn_nnn'nGGC Cuts at: 0 910 1020 Size: 910 110 BglII A'GATC_T Cuts at: 0 170 1010 1020 Size: 170 840 10 Fragments arranged by size: 840 170 10 Bme1580I G_kGCm'C Cuts at: 0 161 193 501 1020 Size: 161 32 308 519 Fragments arranged by size: 519 308 161 32 BmrI ACTGGGnnnn_n' Cuts at: 0 109 195 1020 Size: 109 86 825 Fragments arranged by size: 825 109 86 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 587 1020 Size: 587 433 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 183 503 572 1020 Size: 183 320 69 448 Fragments arranged by size: 448 320 183 69 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 991 1020 Size: 991 29 BsaI GGTCTCn'nnnn_ Cuts at: 0 508 1020 Size: 508 512 BsaAI yAC'GTr Cuts at: 0 494 1020 Size: 494 526 BsaBI GATnn'nnATC Cuts at: 0 461 1020 Size: 461 559 BsaHI Gr'CG_yC Cuts at: 0 59 740 846 1020 Size: 59 681 106 174 Fragments arranged by size: 681 174 106 59 BsaWI w'CCGG_w Cuts at: 0 541 1020 Size: 541 479 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 346 376 1020 Size: 346 30 644 Fragments arranged by size: 644 346 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 156 279 841 1020 Size: 156 123 562 179 Fragments arranged by size: 562 179 156 123 BseYI C'CCAG_C Cuts at: 0 859 925 1020 Size: 859 66 95 Fragments arranged by size: 859 95 66 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 95 1020 Size: 95 925 BsiEI CG_ry'CG Cuts at: 0 909 1020 Size: 909 111 BsiHKAI G_wGCw'C Cuts at: 0 782 1020 Size: 782 238 BsmBI CGTCTCn'nnnn_ Cuts at: 0 50 603 1020 Size: 50 553 417 Fragments arranged by size: 553 417 50 Bsp1286I G_dGCh'C Cuts at: 0 161 193 501 782 1020 Size: 161 32 308 281 238 Fragments arranged by size: 308 281 238 161 32 BspHI T'CATG_A Cuts at: 0 299 560 782 1020 Size: 299 261 222 238 Fragments arranged by size: 299 261 238 222 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 754 904 1020 Size: 754 150 116 Fragments arranged by size: 754 150 116 BsrBI CCG'CTC Cuts at: 0 842 1020 Size: 842 178 BsrDI GCAATG_nn' Cuts at: 0 720 1020 Size: 720 300 BsrFI r'CCGG_y Cuts at: 0 517 653 1020 Size: 517 136 367 Fragments arranged by size: 517 367 136 BssSI C'ACGA_G Cuts at: 0 138 383 673 1020 Size: 138 245 290 347 Fragments arranged by size: 347 290 245 138 BstEII G'GTnAC_C Cuts at: 0 430 1020 Size: 430 590 BstYI r'GATC_y Cuts at: 0 170 1010 1020 Size: 170 840 10 Fragments arranged by size: 840 170 10 BtsI GCAGTG_nn' Cuts at: 0 148 1020 Size: 148 872 DraIII CAC_nnn'GTG Cuts at: 0 494 1020 Size: 494 526 DrdI GACnn_nn'nnGTC Cuts at: 0 940 1020 Size: 940 80 EaeI y'GGCC_r Cuts at: 0 164 519 669 1020 Size: 164 355 150 351 Fragments arranged by size: 355 351 164 150 EcoICRI GAG'CTC Cuts at: 0 780 1020 Size: 780 240 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 183 503 572 602 1020 Size: 183 320 69 30 418 Fragments arranged by size: 418 320 183 69 30 EcoO109I rG'GnC_Cy Cuts at: 0 989 1020 Size: 989 31 FspI TGC'GCA Cuts at: 0 136 1020 Size: 136 884 FspAI rTGC'GCAy Cuts at: 0 136 1020 Size: 136 884 HaeII r_GCGC'y Cuts at: 0 849 1020 Size: 849 171 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 581 613 1020 Size: 581 32 407 Fragments arranged by size: 581 407 32 KasI G'GCGC_C Cuts at: 0 845 1020 Size: 845 175 MscI TGG'CCA Cuts at: 0 166 671 1020 Size: 166 505 349 Fragments arranged by size: 505 349 166 MslI CAynn'nnrTG Cuts at: 0 976 1020 Size: 976 44 MspA1I CmG'CkG Cuts at: 0 947 1020 Size: 947 73 NaeI GCC'GGC Cuts at: 0 655 1020 Size: 655 365 NarI GG'CG_CC Cuts at: 0 846 1020 Size: 846 174 NdeI CA'TA_TG Cuts at: 0 312 1020 Size: 312 708 NgoMIV G'CCGG_C Cuts at: 0 653 1020 Size: 653 367 NsiI A_TGCA'T Cuts at: 0 720 1020 Size: 720 300 NspI r_CATG'y Cuts at: 0 74 651 1020 Size: 74 577 369 Fragments arranged by size: 577 369 74 PflMI CCAn_nnn'nTGG Cuts at: 0 185 629 1020 Size: 185 444 391 Fragments arranged by size: 444 391 185 PfoI T'CCnGG_A Cuts at: 0 452 1020 Size: 452 568 PmlI CAC'GTG Cuts at: 0 494 1020 Size: 494 526 PpuMI rG'GwC_Cy Cuts at: 0 989 1020 Size: 989 31 PstI C_TGCA'G Cuts at: 0 751 1020 Size: 751 269 PvuII CAG'CTG Cuts at: 0 947 1020 Size: 947 73 SacI G_AGCT'C Cuts at: 0 782 1020 Size: 782 238 SbfI CC_TGCA'GG Cuts at: 0 751 1020 Size: 751 269 SfcI C'TryA_G Cuts at: 0 747 1020 Size: 747 273 SfoI GGC'GCC Cuts at: 0 847 1020 Size: 847 173 SmlI C'TyrA_G Cuts at: 0 40 1006 1020 Size: 40 966 14 Fragments arranged by size: 966 40 14 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 57 89 1020 Size: 57 32 931 Fragments arranged by size: 931 57 32 Tth111I GACn'n_nGTC Cuts at: 0 987 1020 Size: 987 33 Enzymes that do cut: AarI AcuI AflII AflIII AhdI BanI BanII BbeI BfrBI BglI BglII Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI BstEII BstYI BtsI DraIII DrdI EaeI EcoICRI Eco57MI EcoO109I FspI FspAI HaeII Hin4I KasI MscI MslI MspA1I NaeI NarI NdeI NgoMIV NsiI NspI PflMI PfoI PmlI PpuMI PstI PvuII SacI SbfI SfcI SfoI SmlI TstI Tth111I Enzymes that do not cut: AatII AccI Acc65I AclI AfeI AgeI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI AvrII BaeI BamHI BbsI BbvCI BcgI BciVI BclI BlpI BmgBI BmtI Bpu10I BsiWI BsmI BspEI BsrGI BssHII BstAPI BstBI BstXI BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraI EagI EarI EciI EcoNI EcoRI EcoRV FalI FseI HincII HindIII HpaI KpnI MfeI MluI MmeI NcoI NheI NotI NruI PacI PasI PciI PmeI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI SapI ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI StyI SwaI TaqII TatI XbaI XcmI XhoI XmaI XmnI ZraI